Restriction map for pF1KB1389
##################################
# NO CUT ENZYMES (  103 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AgeI        AhdI        AocI        
Aor51HI     ApoI        AseI        AsnI        Asp718I     
AspEI       AspI        AvrII       BamHI       BbrPI       
BciVI       BclI        BfrI        BglII       BlnI        
BsaBI       BsaI        BsaWI       BseAI       BsiWI       
BsmBI       BsmI        BspDI       BspEI       BspLU11I    
BsrDI       Bst1107I    BstBI       BstEII      BstPI       
BstZ17I     Bsu36I      ClaI        CpoI        DraI        
Eam1105I    Eco47III    Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
HindIII     HinfI       HpaI        KpnI        KspI        
MamI        MfeI        MluI        MroI        MunI        
NcoI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshBI       Psp1406I    PstI        PvuI        
RsrII       SacII       SalI        SbfI        ScaI        
SfiI        SfuI        SpeI        SphI        Sse8387I    
SspI        SwaI        TfiI        Tsp45I      Tsp509I     
Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AflIII        695  ACACGT               A^C[GA][CT]GT
AscI          153  GGCGCGCC             GG^CGCGCC
Asp700I       609  GAACAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII        1607  TGCGCA               TGC^GCA
BcgI          374  GCACGGACATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI        1020  TACGTA               [CT]AC^GT[GA]
BsiEI        1091  CGGCCG               CG[GA][CT]^CG
BsmAI        1933  GAGAC                GTCTC(1/5)
Bsp1407I     1631  TGTACA               T^GTACA
BspMI         410  ACCTGC               ACCTGC(4/8)
BsrGI        1631  TGTACA               T^GTACA
BstAPI        546  GCAGGCGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI        1862  GGATCT               [GA]^GATC[CT]
DraII         243  GGGGCCC              [GA]G^G[ACGT]CC[CT]
DraIII        896  CACCTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1798  GACTTCACGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         1091  CGGCCG               C^GGCCG
EclXI        1091  CGGCCG               C^GGCCG
Eco52I       1091  CGGCCG               C^GGCCG
EcoO109I      243  GGGGCCC              [GA]G^G[ACGT]CC[CT]
EcoT14I       911  CCAAGG               C^C[AT][AT]GG
FspI         1607  TGCGCA               TGC^GCA
HincII        136  GTTGAC               GT[CT]^[GA]AC
HindII        136  GTTGAC               GT[CT]^[GA]AC
MaeIII       1085  GTTAC                ^GT[ACGT]AC
MflI         1862  GGATCT               [GA]^GATC[CT]
NspI         1034  ACATGC               [GA]CATG^[CT]
PshAI        1935  GACTACAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI          493  GAGCTC               GAGCT^C
SfcI         1937  CTACAG               C^T[GA][CT]AG
SnaBI        1020  TACGTA               TAC^GTA
SspBI        1631  TGTACA               T^GTACA
StuI         1679  AGGCCT               AGG^CCT
StyI          911  CCAAGG               C^C[AT][AT]GG
TaqI         1322  TCGA                 T^CGA
TthHB8I      1322  TCGA                 T^CGA
XhoII        1862  GGATCT               [GA]^GATC[CT]
XmnI          609  GAACAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AlwI         1266  GATCC                GGATC(4/5)
             1862  GGATC               
AlwNI         160  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              547  CAGGCGCTG           
BalI          628  TGGCCA               TGG^CCA
             1244  TGGCCA              
BfaI          469  CTAG                 C^TAG
              541  CTAG                
BlpI          490  GCTGAGC              GC^T[ACGT]AGC
             1419  GCTGAGC             
Bpu1102I      490  GCTGAGC              GC^T[ACGT]AGC
             1419  GCTGAGC             
BsgI         1206  CTGCAC               GTGCAG(16/14)
              478  GTGCAG              
BssSI        1312  CACGAG               CACGAG(-5/-1)
             1905  CACGAG              
BstXI         407  CCAACCTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              733  CCAGAGTTCTGG        
CelII         490  GCTGAGC              GC^T[ACGT]AGC
             1419  GCTGAGC             
MaeI          469  CTAG                 C^TAG
              541  CTAG                
MluNI         628  TGGCCA               TGG^CCA
             1244  TGGCCA              
MscI          628  TGGCCA               TGG^CCA
             1244  TGGCCA              
MseI         1445  TTAA                 T^TAA
             1817  TTAA                
MslI         1089  CACGGCCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1554  CACCATCATG          
PflMI         867  CCACTTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1253  CCATCTGGTGG         
PvuII         160  CAGCTG               CAG^CTG
             1704  CAGCTG              
SapI         1005  GCTCTTC              GCTCTTC(1/4)
             1539  GCTCTTC             
SexAI        1025  ACCTGGT              A^CC[AT]GGT
             1279  ACCTGGT             
Tru9I        1445  TTAA                 T^TAA
             1817  TTAA                
Van91I        867  CCACTTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1253  CCATCTGGTGG         
XcmI         1379  CCATCACCATCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1385  CCATCCTGGCGCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   95 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
Alw44I      ApaI        ApaLI       AspHI       AvaI        
AvaII       BanI        BanII       BbeI        BbsI        
BbvCI       BbvI        BcnI        BglI        BmyI        
BpmI        BpuAI       BsaHI       BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmFI       BsoBI       
Bsp1286I    BspHI       BsrBI       BsrFI       BsrI        
BssHII      BssKI       BstF5I      BstNI       BstUI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DsaI        EaeI        EarI        EcoRII      
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HpaII       HphI        ItaI        KasI        Ksp632I     
MaeII       MboI        MboII       MnlI        MspA1I      
MspI        MvaI        MvnI        MwoI        NaeI        
NarI        NciI        NdeII       NgoMIV      NlaIII      
NlaIV       RcaI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfoI        SgrAI       SmaI        
SmlI        TseI        TspRI       XmaCI       XmaI        
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