Restriction map for pF1KB1389
 (Linear) (Six-base) MAPSORT of: KIBB1389.seq  Check: 287  from: 1  to: 1944



KIBB1389.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:36  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1029    1221    1605    1944 
   Size:       1029     192     384     339

  Fragments arranged by size: 

                1029     384     339     192
 
AflIII A'CryG_T

Cuts at:      0     695    1944 
   Size:        695    1249
 
AleI CACnn'nnGTG

Cuts at:      0    1093    1944 
   Size:       1093     851
 
AlwNI CAG_nnn'CTG

Cuts at:      0     165     552    1944 
   Size:        165     387    1392

  Fragments arranged by size: 

                1392     387     165
 
ApaI G_GGCC'C

Cuts at:      0     216     248     941    1944 
   Size:        216      32     693    1003

  Fragments arranged by size: 

                1003     693     216      32
 
ApaLI G'TGCA_C

Cuts at:      0     372     451     531     661    1944 
   Size:        372      79      80     130    1283

  Fragments arranged by size: 

                1283     372     130      80      79
 
AscI GG'CGCG_CC

Cuts at:      0     154    1944 
   Size:        154    1790
 
AvaI C'yCGr_G

Cuts at:      0      29     247     789     940    1944 
   Size:         29     218     542     151    1004

  Fragments arranged by size: 

                1004     542     218     151      29
 
BanI G'GyrC_C

Cuts at:      0      15     667     709     773     796     846    1189    1479 
   Size:         15     652      42      64      23      50     343     290

Cuts at:   1479    1573    1768    1944 
   Size:         94     195     176

  Fragments arranged by size: 

                 652     343     290     195     176      94      64      50
                  42      23      15
 
BanII G_rGCy'C

Cuts at:      0     216     248     497     926     941    1795    1944 
   Size:        216      32     249     429      15     854     149

  Fragments arranged by size: 

                 854     429     249     216     149      32      15
 
BbeI G_GCGC'C

Cuts at:      0      19     671     800    1483    1772    1944 
   Size:         19     652     129     683     289     172

  Fragments arranged by size: 

                 683     652     289     172     129      19
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1136    1611    1800    1944 
   Size:       1136     475     189     144

  Fragments arranged by size: 

                1136     475     189     144
 
BbvCI CC'TCA_GC

Cuts at:      0       5     281     755    1944 
   Size:          5     276     474    1189

  Fragments arranged by size: 

                1189     474     276       5
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     363     397    1944 
   Size:        363      34    1547

  Fragments arranged by size: 

                1547     363      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0      88     220    1357    1944 
   Size:         88     132    1137     587

  Fragments arranged by size: 

                1137     587     132      88
 
BlpI GC'TnA_GC

Cuts at:      0     491    1420    1944 
   Size:        491     929     524

  Fragments arranged by size: 

                 929     524     491
 
Bme1580I G_kGCm'C

Cuts at:      0     216     248     376     455     535     665     712     776 
   Size:        216      32     128      79      80     130      47      64

Cuts at:    776     851     941    1173    1192    1944 
   Size:         75      90     232      19     752

  Fragments arranged by size: 

                 752     232     216     130     128      90      80      79
                  75      64      47      32      19
 
BmgBI CAC'GTC

Cuts at:      0     698    1511    1850    1944 
   Size:        698     813     339      94

  Fragments arranged by size: 

                 813     698     339      94
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     205     466    1612    1738    1911    1944 
   Size:        205     261    1146     126     173      33

  Fragments arranged by size: 

                1146     261     205     173     126      33
 
Bpu10I CC'TnA_GC

Cuts at:      0       5     281     755    1944 
   Size:          5     276     474    1189

  Fragments arranged by size: 

                1189     474     276       5
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     481     625     826    1944 
   Size:        481     144     201    1118

  Fragments arranged by size: 

                1118     481     201     144
 
BsaAI yAC'GTr

Cuts at:      0    1022    1944 
   Size:       1022     922
 
BsaHI Gr'CG_yC

Cuts at:      0      16     110     668     718     797    1480    1616    1769 
   Size:         16      94     558      50      79     683     136     153

Cuts at:   1769    1944 
   Size:        175

  Fragments arranged by size: 

                 683     558     175     153     136      94      79      50
                  16
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      22      25     109     958    1944 
   Size:         22       3      84     849     986

  Fragments arranged by size: 

                 986     849      84      22       3
 
BseYI C'CCAG_C

Cuts at:      0      53     201     414    1395    1706    1944 
   Size:         53     148     213     981     311     238

  Fragments arranged by size: 

                 981     311     238     213     148      53
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     499    1191    1944 
   Size:        499     692     753

  Fragments arranged by size: 

                 753     692     499
 
BsiEI CG_ry'CG

Cuts at:      0    1094    1944 
   Size:       1094     850
 
BsiHKAI G_wGCw'C

Cuts at:      0     376     455     497     514     535     665    1944 
   Size:        376      79      42      17      21     130    1279

  Fragments arranged by size: 

                1279     376     130      79      42      21      17
 
Bsp1286I G_dGCh'C

Cuts at:      0     216     248     376     455     497     514     535     665 
   Size:        216      32     128      79      42      17      21     130

Cuts at:    665     712     776     851     926     941    1173    1192    1795 
   Size:         47      64      75      75      15     232      19     603

Cuts at:   1795    1944 
   Size:        149

  Fragments arranged by size: 

                 603     232     216     149     130     128      79      75
                  75      64      47      42      32      21      19      17
                  15
 
BspHI T'CATG_A

Cuts at:      0     605    1220    1559    1811    1944 
   Size:        605     615     339     252     133

  Fragments arranged by size: 

                 615     605     339     252     133
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     419    1944 
   Size:        419    1525
 
BsrBI CCG'CTC

Cuts at:      0     296     318     566    1059    1424    1944 
   Size:        296      22     248     493     365     520

  Fragments arranged by size: 

                 520     493     365     296     248      22
 
BsrFI r'CCGG_y

Cuts at:      0      37     112     670     799    1114    1365    1525    1944 
   Size:         37      75     558     129     315     251     160     419

  Fragments arranged by size: 

                 558     419     315     251     160     129      75      37
            
 
BsrGI T'GTAC_A

Cuts at:      0    1631    1944 
   Size:       1631     313
 
BssHII G'CGCG_C

Cuts at:      0     154     579     581     583     834    1944 
   Size:        154     425       2       2     251    1110

  Fragments arranged by size: 

                1110     425     251     154       2       2
 
BssSI C'ACGA_G

Cuts at:      0    1312    1905    1944 
   Size:       1312     593      39

  Fragments arranged by size: 

                1312     593      39
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     552    1944 
   Size:        552    1392
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     414     740    1944 
   Size:        414     326    1204

  Fragments arranged by size: 

                1204     414     326
 
BstYI r'GATC_y

Cuts at:      0    1862    1944 
   Size:       1862      82
 
BtgI C'CryG_G

Cuts at:      0     339     777    1094    1100    1944 
   Size:        339     438     317       6     844

  Fragments arranged by size: 

                 844     438     339     317       6
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     366    1095    1427    1641    1944 
   Size:        366     729     332     214     303

  Fragments arranged by size: 

                 729     366     332     303     214
 
BtsI GCAGTG_nn'

Cuts at:      0     487    1944 
   Size:        487    1457
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     328     363    1944 
   Size:        328      35    1581

  Fragments arranged by size: 

                1581     328      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     901    1944 
   Size:        901    1043
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1804    1944 
   Size:       1804     140
 
EaeI y'GGCC_r

Cuts at:      0     117     628    1091    1097    1244    1349    1367    1523 
   Size:        117     511     463       6     147     105      18     156

Cuts at:   1523    1944 
   Size:        421

  Fragments arranged by size: 

                 511     463     421     156     147     117     105      18
                   6
 
EagI C'GGCC_G

Cuts at:      0    1091    1944 
   Size:       1091     853
 
EarI CTCTTCn'nnn_

Cuts at:      0    1012    1222    1492    1546    1944 
   Size:       1012     210     270      54     398

  Fragments arranged by size: 

                1012     398     270     210      54
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     106     782    1778    1944 
   Size:        106     676     996     166

  Fragments arranged by size: 

                 996     676     166     106
 
EcoICRI GAG'CTC

Cuts at:      0     495    1944 
   Size:        495    1449
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     205     466    1029    1221    1605    1612    1738    1911 
   Size:        205     261     563     192     384       7     126     173

Cuts at:   1911    1944 
   Size:         33

  Fragments arranged by size: 

                 563     384     261     205     192     173     126      33
                   7
 
EcoO109I rG'GnC_Cy

Cuts at:      0     244    1944 
   Size:        244    1700
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     636    1944 
   Size:        636    1308
 
FspI TGC'GCA

Cuts at:      0    1609    1944 
   Size:       1609     335
 
FspAI rTGC'GCAy

Cuts at:      0    1609    1944 
   Size:       1609     335
 
HaeII r_GCGC'y

Cuts at:      0      19      76     553     604     671     800    1044    1396 
   Size:         19      57     477      51      67     129     244     352

Cuts at:   1396    1483    1772    1944 
   Size:         87     289     172

  Fragments arranged by size: 

                 477     352     289     244     172     129      87      67
                  57      51      19
 
HincII GTy'rAC

Cuts at:      0     138    1944 
   Size:        138    1806
 
KasI G'GCGC_C

Cuts at:      0      15     667     796    1479    1768    1944 
   Size:         15     652     129     683     289     176

  Fragments arranged by size: 

                 683     652     289     176     129      15
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     725     788    1944 
   Size:        725      63    1156

  Fragments arranged by size: 

                1156     725      63
 
MscI TGG'CCA

Cuts at:      0     630    1246    1944 
   Size:        630     616     698

  Fragments arranged by size: 

                 698     630     616
 
MslI CAynn'nnrTG

Cuts at:      0    1093    1558    1944 
   Size:       1093     465     386

  Fragments arranged by size: 

                1093     465     386
 
MspA1I CmG'CkG

Cuts at:      0     104     162     195     369     490    1706    1944 
   Size:        104      58      33     174     121    1216     238

  Fragments arranged by size: 

                1216     238     174     121     104      58      33
 
NaeI GCC'GGC

Cuts at:      0      39     672     801    1116    1367    1527    1944 
   Size:         39     633     129     315     251     160     417

  Fragments arranged by size: 

                 633     417     315     251     160     129      39
 
NarI GG'CG_CC

Cuts at:      0      16     668     797    1480    1769    1944 
   Size:         16     652     129     683     289     175

  Fragments arranged by size: 

                 683     652     289     175     129      16
 
NgoMIV G'CCGG_C

Cuts at:      0      37     670     799    1114    1365    1525    1944 
   Size:         37     633     129     315     251     160     419

  Fragments arranged by size: 

                 633     419     315     251     160     129      37
 
NspI r_CATG'y

Cuts at:      0    1038    1944 
   Size:       1038     906
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     873    1259    1944 
   Size:        873     386     685

  Fragments arranged by size: 

                 873     685     386
 
PfoI T'CCnGG_A

Cuts at:      0     332    1124    1685    1888    1944 
   Size:        332     792     561     203      56

  Fragments arranged by size: 

                 792     561     332     203      56
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     484     516    1944 
   Size:        484      32    1428

  Fragments arranged by size: 

                1428     484      32
 
PshAI GACnn'nnGTC

Cuts at:      0    1939    1944 
   Size:       1939       5
 
PspOMI G'GGCC_C

Cuts at:      0     212     244     937    1944 
   Size:        212      32     693    1007

  Fragments arranged by size: 

                1007     693     212      32
 
PvuII CAG'CTG

Cuts at:      0     162    1706    1944 
   Size:        162    1544     238

  Fragments arranged by size: 

                1544     238     162
 
SacI G_AGCT'C

Cuts at:      0     497    1944 
   Size:        497    1447
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1012    1546    1944 
   Size:       1012     534     398

  Fragments arranged by size: 

                1012     534     398
 
SexAI A'CCwGG_T

Cuts at:      0    1025    1279    1944 
   Size:       1025     254     665

  Fragments arranged by size: 

                1025     665     254
 
SfcI C'TryA_G

Cuts at:      0    1937    1944 
   Size:       1937       7
 
SfoI GGC'GCC

Cuts at:      0      17     669     798    1481    1770    1944 
   Size:         17     652     129     683     289     174

  Fragments arranged by size: 

                 683     652     289     174     129      17
 
SgrAI Cr'CCGG_yG

Cuts at:      0      37     112     670     799    1944 
   Size:         37      75     558     129    1145

  Fragments arranged by size: 

                1145     558     129      75      37
 
SmaI CCC'GGG

Cuts at:      0      31     249     791    1944 
   Size:         31     218     542    1153

  Fragments arranged by size: 

                1153     542     218      31
 
SmlI C'TyrA_G

Cuts at:      0     496     640     841    1944 
   Size:        496     144     201    1103

  Fragments arranged by size: 

                1103     496     201     144
 
SnaBI TAC'GTA

Cuts at:      0    1022    1944 
   Size:       1022     922
 
SrfI GCCC'GGGC

Cuts at:      0     249    1944 
   Size:        249    1695
 
StuI AGG'CCT

Cuts at:      0    1681    1944 
   Size:       1681     263
 
StyI C'CwwG_G

Cuts at:      0     911    1944 
   Size:        911    1033
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1148    1251    1597    1698    1944 
   Size:       1148     103     346     101     246

  Fragments arranged by size: 

                1148     346     246     103     101
 
TatI w'GTAC_w

Cuts at:      0     305     929    1631    1944 
   Size:        305     624     702     313

  Fragments arranged by size: 

                 702     624     313     305
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1386    1392    1944 
   Size:       1386       6     552

  Fragments arranged by size: 

                1386     552       6
 
XmaI C'CCGG_G

Cuts at:      0      29     247     789    1944 
   Size:         29     218     542    1155

  Fragments arranged by size: 

                1155     542     218      29
 
XmnI GAAnn'nnTTC

Cuts at:      0     613    1944 
   Size:        613    1331
 

 Enzymes that do cut:

     AcuI   AflIII     AleI    AlwNI     ApaI    ApaLI     AscI     AvaI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI     BglI     BlpI
 Bme1580I    BmgBI     BpmI   Bpu10I    BpuEI    BsaAI    BsaHI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspHI    BspMI    BsrBI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstXI    BstYI     BtgI
    BtgZI     BtsI    CspCI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I     FalI     FspI    FspAI    HaeII
   HincII     KasI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
   NgoMIV     NspI    PflMI     PfoI     PpiI    PshAI   PspOMI    PvuII
     SacI     SapI    SexAI     SfcI     SfoI    SgrAI     SmaI     SmlI
    SnaBI     SrfI     StuI     StyI    TaqII     TatI     XcmI     XmaI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI     ApoI     AseI    AsiSI    AvrII     BaeI
    BamHI    BciVI     BclI    BfrBI    BglII     BmrI     BmtI     BplI
     BsaI    BsaBI    BsaWI    BsaXI    BsiWI     BsmI    BsmBI    BspEI
    BsrDI    BstBI   BstEII  BstZ17I   Bsu36I     ClaI     DraI    EcoNI
    EcoRI    EcoRV     FseI    Hin4I  HindIII     HpaI     KpnI     MfeI
     MluI     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI     PmeI     PmlI    PpuMI     PsiI    PspXI     PsrI
     PstI     PvuI    RsrII    SacII     SalI    SanDI     SbfI     ScaI
     SfiI     SgfI     SpeI     SphI     SspI     SwaI     TstI  Tth111I
     XbaI     XhoI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com