Restriction map for pF1KB1338
 (Circular) (Six-base) MAPSORT of: pF1KB1338.seq  Check: 1541  from: 1  to: 4928



pF1KB1338  4928 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:22  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    483     483 
   Size:       4928
 
AatII G_ACGT'C

Cuts at:    727     727 
   Size:       4928
 
AccI GT'mk_AC

Cuts at:   1966    1966 
   Size:       4928
 
Acc65I G'GTAC_C

Cuts at:   1944    1944 
   Size:       4928
 
AclI AA'CG_TT

Cuts at:   4768    4768 
   Size:       4928
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    594    2753    3185    3538    4200     594 
   Size:       2159     432     353     662    1322

  Fragments arranged by size: 

                2159    1322     662     432     353
 
AfeI AGC'GCT

Cuts at:   1620    1620 
   Size:       4928
 
AflIII A'CryG_T

Cuts at:    326    2280    4080    4361     326 
   Size:       1954    1800     281     893

  Fragments arranged by size: 

                1954    1800     893     281
 
AhdI GACnn_n'nnGTC

Cuts at:   1062    1062 
   Size:       4928
 
AlwNI CAG_nnn'CTG

Cuts at:   1350    1854    2233    3671    1350 
   Size:        504     379    1438    2607

  Fragments arranged by size: 

                2607    1438     504     379
 
ApaI G_GGCC'C

Cuts at:    211    1175    1197     211 
   Size:        964      22    3942

  Fragments arranged by size: 

                3942     964      22
 
ApaLI G'TGCA_C

Cuts at:    148    3766     148 
   Size:       3618    1310
 
ApoI r'AATT_y

Cuts at:   1932    4281    4537    1932 
   Size:       2349     256    2323

  Fragments arranged by size: 

                2349    2323     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4928
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4928
 
AvaI C'yCGr_G

Cuts at:    644    1496    1513    1948     644 
   Size:        852      17     435    3624

  Fragments arranged by size: 

                3624     852     435      17
 
BamHI G'GATC_C

Cuts at:   1033    1953    1033 
   Size:        920    4008
 
BanI G'GyrC_C

Cuts at:    128    1279    1572    1728    1906    1944    2358    2606    2641 
   Size:       1151     293     156     178      38     414     248      35

Cuts at:   2641     128 
   Size:       2415

  Fragments arranged by size: 

                2415    1151     414     293     248     178     156      38
                  35
 
BanII G_rGCy'C

Cuts at:    211     373     652    1142    1175    1197    1741    1873    1942 
   Size:        162     279     490      33      22     544     132      69

Cuts at:   1942    2972     211 
   Size:       1030    2167

  Fragments arranged by size: 

                2167    1030     544     490     279     162     132      69
                  33      22
 
BbeI G_GCGC'C

Cuts at:    132    1732    2362    2610     132 
   Size:       1600     630     248    2450

  Fragments arranged by size: 

                2450    1600     630     248
 
BbsI GAAGACnn'nnnn_

Cuts at:   1812    1812 
   Size:       4928
 
BbvCI CC'TCA_GC

Cuts at:   1533    1533 
   Size:       4928
 
BciVI GTATCCnnnnn_n'

Cuts at:   2826    3882    4928    2826 
   Size:       1056    1046    2826

  Fragments arranged by size: 

                2826    1056    1046
 
BclI T'GATC_A

Cuts at:   2448    2448 
   Size:       4928
 
BglI GCCn_nnn'nGGC

Cuts at:    623    1170    1712     623 
   Size:        547     542    3839

  Fragments arranged by size: 

                3839     547     542
 
BglII A'GATC_T

Cuts at:   2443    4480    2443 
   Size:       2037    2891
 
BlpI GC'TnA_GC

Cuts at:   1774    1872    2040    1774 
   Size:         98     168    4662

  Fragments arranged by size: 

                4662     168      98
 
Bme1580I G_kGCm'C

Cuts at:    152     211    1175    1197    2553    2646    3770     152 
   Size:         59     964      22    1356      93    1124    1310

  Fragments arranged by size: 

                1356    1310    1124     964      93      59      22
 
BmrI ACTGGGnnnn_n'

Cuts at:   1150    1238    1403    2140    2221    2556    4700    1150 
   Size:         88     165     737      81     335    2144    1378

  Fragments arranged by size: 

                2144    1378     737     335     165      88      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3295    3295 
   Size:       4928
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    595     724    1276    1295    1360     595 
   Size:        129     552      19      65    4163

  Fragments arranged by size: 

                4163     552     129      65      19
 
Bpu10I CC'TnA_GC

Cuts at:   1533    1533 
   Size:       4928
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    346     409     493     535    2104    2983    3697    3995     346 
   Size:         63      84      42    1569     879     714     298    1279

  Fragments arranged by size: 

                1569    1279     879     714     298      84      63      42
            
 
BsaI GGTCTCn'nnnn_

Cuts at:   1070    1252    1318    1391    1618    4632    1070 
   Size:        182      66      73     227    3014    1366

  Fragments arranged by size: 

                3014    1366     227     182      73      66
 
BsaAI yAC'GTr

Cuts at:    327    2281     327 
   Size:       1954    2974
 
BsaBI GATnn'nnATC

Cuts at:      6    2442    4479       6 
   Size:       2436    2037     455

  Fragments arranged by size: 

                2436    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    129     467     724    1729    2359    2607    3309    4805     129 
   Size:        338     257    1005     630     248     702    1496     252

  Fragments arranged by size: 

                1496    1005     702     630     338     257     252     248
            
 
BsaWI w'CCGG_w

Cuts at:   2117    2638    3316    3727    3874    4148    2117 
   Size:        521     678     411     147     274    2897

  Fragments arranged by size: 

                2897     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    142     172     625     655     685     781     811     142 
   Size:         30     453      30      30      96      30    4259

  Fragments arranged by size: 

                4259     453      96      30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    532     660     663     790     907     910    1119    1122     532 
   Size:        128       3     127     117       3     209       3    4338

  Fragments arranged by size: 

                4338     209     128     127     117       3       3       3
            
 
BseYI C'CCAG_C

Cuts at:    603    1168    1222    1366    1402    1478    1701    2353    3111 
   Size:        565      54     144      36      76     223     652     758

Cuts at:   3111    3776    4184     603 
   Size:        665     408    1347

  Fragments arranged by size: 

                1347     758     665     652     565     408     223     144
                  76      54      36
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    854     854 
   Size:       4928
 
BsiEI CG_ry'CG

Cuts at:    104     447    2470    2516    3746     104 
   Size:        343    2023      46    1230    1286

  Fragments arranged by size: 

                2023    1286    1230     343      46
 
BsiHKAI G_wGCw'C

Cuts at:    152    1873    1942    2720    2910    3770     152 
   Size:       1721      69     778     190     860    1310

  Fragments arranged by size: 

                1721    1310     860     778     190      69
 
BsmBI CGTCTCn'nnnn_

Cuts at:   1648    1648 
   Size:       4928
 
Bsp1286I G_dGCh'C

Cuts at:    152     211     373     652    1142    1175    1197    1741    1873 
   Size:         59     162     279     490      33      22     544     132

Cuts at:   1873    1942    2553    2646    2720    2910    2972    3770     152 
   Size:         69     611      93      74     190      62     798    1310

  Fragments arranged by size: 

                1310     798     611     544     490     279     190     162
                 132      93      74      69      62      59      33      22
            
 
BspHI T'CATG_A

Cuts at:   3360    3360 
   Size:       4928
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    365     483     960    1978    2192    2494    2875     365 
   Size:        118     477    1018     214     302     381    2418

  Fragments arranged by size: 

                2418    1018     477     381     302     214     118
 
BsrBI CCG'CTC

Cuts at:     50     378     522     641    1773    3220    3274    4758    4923 
   Size:        328     144     119    1132    1447      54    1484     165

Cuts at:   4923      50 
   Size:         55

  Fragments arranged by size: 

                1484    1447    1132     328     165     144     119      55
                  54
 
BsrDI GCAATG_nn'

Cuts at:    201     343    2840     201 
   Size:        142    2497    2289

  Fragments arranged by size: 

                2497    2289     142
 
BsrFI r'CCGG_y

Cuts at:   1687    1695    1903    2926    3107    1687 
   Size:          8     208    1023     181    3508

  Fragments arranged by size: 

                3508    1023     208     181       8
 
BsrGI T'GTAC_A

Cuts at:    329     329 
   Size:       4928
 
BssSI C'ACGA_G

Cuts at:   3199    3907    3199 
   Size:        708    4220
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1350    1854    2305    1350 
   Size:        504     451    3973

  Fragments arranged by size: 

                3973     504     451
 
BstBI TT'CG_AA

Cuts at:   3289    3289 
   Size:       4928
 
BstYI r'GATC_y

Cuts at:      1    1033    1110    1953    2420    2443    2778    3024    3330 
   Size:       1032      77     843     467      23     335     246     306

Cuts at:   3330    3342    3428    4480       1 
   Size:         12      86    1052     449

  Fragments arranged by size: 

                1052    1032     843     467     449     335     306     246
                  86      77      23      12
 
Bsu36I CC'TnA_GG

Cuts at:   1077    1077 
   Size:       4928
 
BtgI C'CryG_G

Cuts at:    821     842     991    1882     821 
   Size:         21     149     891    3867

  Fragments arranged by size: 

                3867     891     149      21
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2878    3059    3222    2878 
   Size:        181     163    4584

  Fragments arranged by size: 

                4584     181     163
 
BtsI GCAGTG_nn'

Cuts at:    600     975    2171     600 
   Size:        375    1196    3357

  Fragments arranged by size: 

                3357    1196     375
 
DraI TTT'AAA

Cuts at:   1927    1927 
   Size:       4928
 
DraIII CAC_nnn'GTG

Cuts at:   1276    1276 
   Size:       4928
 
DrdI GACnn_nn'nnGTC

Cuts at:    466    1515    1564    2634    3978     466 
   Size:       1049      49    1070    1344    1416

  Fragments arranged by size: 

                1416    1344    1070    1049      49
 
EaeI y'GGCC_r

Cuts at:    824    2513    2687    3078    3105    4450     824 
   Size:       1689     174     391      27    1345    1302

  Fragments arranged by size: 

                1689    1345    1302     391     174      27
 
EagI C'GGCC_G

Cuts at:   2513    2513 
   Size:       4928
 
EarI CTCTTCn'nnn_

Cuts at:    681     728     875    2951    3161     681 
   Size:         47     147    2076     210    2448

  Fragments arranged by size: 

                2448    2076     210     147      47
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   3136    3878    4024    4738    3136 
   Size:        742     146     714    3326

  Fragments arranged by size: 

                3326     742     714     146
 
EcoICRI GAG'CTC

Cuts at:   1871    1940    1871 
   Size:         69    4859
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    594     595     724    1276    1295    1360    2753    3185    3538 
   Size:          1     129     552      19      65    1393     432     353

Cuts at:   3538    4200     594 
   Size:        662    1322

  Fragments arranged by size: 

                1393    1322     662     552     432     353     129      65
                  19       1
 
EcoNI CCTnn'n_nnAGG

Cuts at:    508     706    1146     508 
   Size:        198     440    4290

  Fragments arranged by size: 

                4290     440     198
 
EcoO109I rG'GnC_Cy

Cuts at:    207     208     797    1045    1151    1172    1194    1347    1528 
   Size:          1     589     248     106      21      22     153     181

Cuts at:   1528    2067     207 
   Size:        539    3068

  Fragments arranged by size: 

                3068     589     539     248     181     153     106      22
                  21       1
 
EcoRI G'AATT_C

Cuts at:   1932    1932 
   Size:       4928
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2947    2947 
   Size:       4928
 
FspI TGC'GCA

Cuts at:   1684    2709    1684 
   Size:       1025    3903
 
FspAI rTGC'GCAy

Cuts at:   1684    1684 
   Size:       4928
 
HaeII r_GCGC'y

Cuts at:    132    1622    1702    1732    1757    2362    2610    3840    4611 
   Size:       1490      80      30      25     605     248    1230     771

Cuts at:   4611     132 
   Size:        449

  Fragments arranged by size: 

                1490    1230     771     605     449     248      80      30
                  25
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    781     813    1048    1080    2770    2800    2802    2832     781 
   Size:         32     235      32    1690      30       2      30    2877

  Fragments arranged by size: 

                2877    1690     235      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:    471    1551    1967     471 
   Size:       1080     416    3432

  Fragments arranged by size: 

                3432    1080     416
 
KasI G'GCGC_C

Cuts at:    128    1728    2358    2606     128 
   Size:       1600     630     248    2450

  Fragments arranged by size: 

                2450    1600     630     248
 
KpnI G_GTAC'C

Cuts at:   1948    1948 
   Size:       4928
 
MluI A'CGCG_T

Cuts at:   4361    4361 
   Size:       4928
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    722    1289    3687    3871     722 
   Size:        567    2398     184    1779

  Fragments arranged by size: 

                2398    1779     567     184
 
MscI TGG'CCA

Cuts at:    826    2689     826 
   Size:       1863    3065
 
MslI CAynn'nnrTG

Cuts at:    280    2917    3044     280 
   Size:       2637     127    2164

  Fragments arranged by size: 

                2637    2164     127
 
MspA1I CmG'CkG

Cuts at:    308     603     747     753     858    1789    1854    1884    2039 
   Size:        295     144       6     105     931      65      30     155

Cuts at:   2039    2353    2713    3497    3742     308 
   Size:        314     360     784     245    1494

  Fragments arranged by size: 

                1494     931     784     360     314     295     245     155
                 144     105      65      30       6
 
NaeI GCC'GGC

Cuts at:   1697    3109    1697 
   Size:       1412    3516
 
NarI GG'CG_CC

Cuts at:    129    1729    2359    2607     129 
   Size:       1600     630     248    2450

  Fragments arranged by size: 

                2450    1600     630     248
 
NcoI C'CATG_G

Cuts at:    821     991     821 
   Size:        170    4758
 
NgoMIV G'CCGG_C

Cuts at:   1695    3107    1695 
   Size:       1412    3516
 
NspI r_CATG'y

Cuts at:    285    1981    2481    4084     285 
   Size:       1696     500    1603    1129

  Fragments arranged by size: 

                1696    1603    1129     500
 
PasI CC'CwG_GG

Cuts at:    203    1106    1142    1190    1734     203 
   Size:        903      36      48     544    3397

  Fragments arranged by size: 

                3397     903     544      48      36
 
PciI A'CATG_T

Cuts at:   4080    4080 
   Size:       4928
 
PfoI T'CCnGG_A

Cuts at:    926     926 
   Size:       4928
 
PmeI GTTT'AAAC

Cuts at:   1927    1927 
   Size:       4928
 
PmlI CAC'GTG

Cuts at:    327     327 
   Size:       4928
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3374    3406    3374 
   Size:         32    4896
 
PpuMI rG'GwC_Cy

Cuts at:    797    1151    1528     797 
   Size:        354     377    4197

  Fragments arranged by size: 

                4197     377     354
 
PspOMI G'GGCC_C

Cuts at:    207    1171    1193     207 
   Size:        964      22    3942

  Fragments arranged by size: 

                3942     964      22
 
PstI C_TGCA'G

Cuts at:    746     752    1056    1975    2660     746 
   Size:          6     304     919     685    3014

  Fragments arranged by size: 

                3014     919     685     304       6
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4928
 
PvuII CAG'CTG

Cuts at:    603     747     753    1789    2353    2713     603 
   Size:        144       6    1036     564     360    2818

  Fragments arranged by size: 

                2818    1036     564     360     144       6
 
RsrII CG'GwC_CG

Cuts at:   3123    3123 
   Size:       4928
 
SacI G_AGCT'C

Cuts at:   1873    1942    1873 
   Size:         69    4859
 
SacII CC_GC'GG

Cuts at:   1885    1885 
   Size:       4928
 
SalI G'TCGA_C

Cuts at:   1965    1965 
   Size:       4928
 
SanDI GG'GwC_CC

Cuts at:   1528    1528 
   Size:       4928
 
SapI GCTCTTCn'nnn_

Cuts at:   2951    3161    2951 
   Size:        210    4718
 
SbfI CC_TGCA'GG

Cuts at:   1975    1975 
   Size:       4928
 
ScaI AGT'ACT

Cuts at:   4445    4445 
   Size:       4928
 
SexAI A'CCwGG_T

Cuts at:   1272    1272 
   Size:       4928
 
SfcI C'TryA_G

Cuts at:     33     633     742     748    1052    1749    1971    2656    3624 
   Size:        600     109       6     304     697     222     685     968

Cuts at:   3624    3815      33 
   Size:        191    1146

  Fragments arranged by size: 

                1146     968     697     685     600     304     222     191
                 109       6
 
SfoI GGC'GCC

Cuts at:    130    1730    2360    2608     130 
   Size:       1600     630     248    2450

  Fragments arranged by size: 

                2450    1600     630     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4928
 
SgrAI Cr'CCGG_yG

Cuts at:   1695    1695 
   Size:       4928
 
SmaI CCC'GGG

Cuts at:    646    1950     646 
   Size:       1304    3624
 
SmlI C'TyrA_G

Cuts at:    361     424     508     550    2083    2998    3712    3974     361 
   Size:         63      84      42    1533     915     714     262    1315

  Fragments arranged by size: 

                1533    1315     915     714     262      84      63      42
            
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4928
 
SphI G_CATG'C

Cuts at:    285    1981    2481     285 
   Size:       1696     500    2732

  Fragments arranged by size: 

                2732    1696     500
 
StyI C'CwwG_G

Cuts at:    557     821     991    1240    1328    1544    2062    2416     557 
   Size:        264     170     249      88     216     518     354    3069

  Fragments arranged by size: 

                3069     518     354     264     249     216     170      88
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   2511    2644    3103    3312    2511 
   Size:        133     459     209    4127

  Fragments arranged by size: 

                4127     459     209     133
 
TatI w'GTAC_w

Cuts at:    329     437    4443     329 
   Size:        108    4006     814

  Fragments arranged by size: 

                4006     814     108
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    142     174    4445    4477     142 
   Size:         32    4271      32     593

  Fragments arranged by size: 

                4271     593      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2725    2725 
   Size:       4928
 
XbaI T'CTAG_A

Cuts at:   1959    1959 
   Size:       4928
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    564     930    1335    1374    2423     564 
   Size:        366     405      39    1049    3069

  Fragments arranged by size: 

                3069    1049     405     366      39
 
XmaI C'CCGG_G

Cuts at:    644    1948     644 
   Size:       1304    3624
 
XmnI GAAnn'nnTTC

Cuts at:   1600    1600 
   Size:       4928
 
ZraI GAC'GTC

Cuts at:    725     725 
   Size:       4928
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII
     AhdI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
     FalI     FspI    FspAI    HaeII    Hin4I   HincII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NspI     PasI     PciI     PfoI     PmeI     PmlI     PpiI
    PpuMI   PspOMI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfoI
     SgfI    SgrAI     SmaI     SmlI     SpeI     SphI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

    AflII     AgeI     AleI     AlfI     AloI     AscI    AvrII     BaeI
     BcgI    BfrBI    BmgBI     BmtI    BsiWI     BsmI    BspEI   BssHII
   BstEII    BstXI  BstZ17I     ClaI    CspCI    EcoRV     FseI  HindIII
     HpaI     MfeI     NdeI     NheI     NotI     NruI     NsiI     PacI
    PflMI    PshAI     PsiI    PspXI     PsrI     SfiI    SnaBI     SrfI
     SspI     StuI     SwaI     XhoI
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