Restriction map for pF1KB1292
##################################
# NO CUT ENZYMES (   95 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AflII       
AflIII      AgeI        AlwNI       AscI        AseI        
AsnI        AspI        AviII       AvrII       BbrPI       
BcgI        BciVI       BclI        BfrI        BglI        
BlnI        BlpI        BpmI        Bpu1102I    BsaAI       
BsaBI       BsaI        BsaWI       BseAI       BsiWI       
BsmI        BspDI       BspEI       BspHI       BspLU11I    
BssHII      Bst1107I    BstAPI      BstBI       BstXI       
BstZ17I     CelII       ClaI        CpoI        DrdI        
EagI        EclXI       Eco52I      EcoRI       EcoRV       
FbaI        FseI        FspI        HincII      HindII      
HindIII     HpaI        KspI        MamI        MfeI        
MluI        MroI        MunI        NaeI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    RcaI        RsrII       
SacI        SacII       SalI        ScaI        SfiI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SwaI        Tth111I     XbaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

Acc65I        391  GGTACC               G^GTACC
AcsI          497  GAATTT               [GA]^AATT[CT]
AhdI         1255  GACACCGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI          321  CCTGAGG              CC^T[ACGT]AGG
Aor51HI      1909  AGCGCT               AGC^GCT
ApaI         1095  GGGCCC               GGGCC^C
ApoI          497  GAATTT               [GA]^AATT[CT]
Asp718I       391  GGTACC               G^GTACC
AspEI        1255  GACACCGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI            2  TGGCCA               TGG^CCA
BbsI          517  GAAGAC               GAAGAC(2/6)
BbvCI        1730  CCTCAGC              CCTCAGC(-5/-2)
BcnI          540  CCGGG                CC^[GC]GG
BfaI          184  CTAG                 C^TAG
BglII        1065  AGATCT               A^GATCT
BpuAI         517  GAAGAC               GAAGAC(2/6)
BsiEI        1718  CGATCG               CG[GA][CT]^CG
BsmBI         244  GAGACG               CGTCTC(1/5)
BsrDI        1511  GCAATG               GCAATG(2/0)
BssSI         758  CACGAG               CACGAG(-5/-1)
BstEII        123  GGTGACC              G^GT[ACGT]ACC
BstPI         123  GGTGACC              G^GT[ACGT]ACC
Bsu36I        321  CCTGAGG              CC^T[ACGT]AGG
DraI          811  TTTAAA               TTT^AAA
DraIII       1033  CACACCGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I     1255  GACACCGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     1909  AGCGCT               AGC^GCT
Eco81I        321  CCTGAGG              CC^T[ACGT]AGG
EcoO65I       123  GGTGACC              G^GT[ACGT]ACC
EcoT22I       141  ATGCAT               ATGCA^T
KpnI          391  GGTACC               GGTAC^C
MaeI          184  CTAG                 C^TAG
MluNI           2  TGGCCA               TGG^CCA
MscI            2  TGGCCA               TGG^CCA
NciI          540  CCGGG                CC^[GC]GG
NdeI         1949  CATATG               CA^TATG
NsiI          141  ATGCAT               ATGCA^T
NspI          139  GCATGC               [GA]CATG^[CT]
PaeR7I       1299  CTCGAG               C^TCGAG
PflMI        1668  CCAGACCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuI         1718  CGATCG               CGAT^CG
PvuII         304  CAGCTG               CAG^CTG
SbfI         1467  CCTGCAGG             CCTGCA^GG
SexAI         822  ACCAGGT              A^CC[AT]GGT
SfaNI         711  GATGC                GCATC(5/9)
SphI          139  GCATGC               GCATG^C
Sse8387I     1467  CCTGCAGG             CCTGCA^GG
SspI          156  AATATT               AAT^ATT
StuI         1638  AGGCCT               AGG^CCT
Van91I       1668  CCAGACCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         1529  CCATCGGTCCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         1299  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AccII        1314  CGCG                 CG^CG
             1397  CGCG                
Alw44I       1039  GTGCAC               G^TGCAC
             1192  GTGCAC              
ApaLI        1039  GTGCAC               G^TGCAC
             1192  GTGCAC              
Asp700I       202  GAAACCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1860  GAACATCTTC          
BamHI        1820  GGATCC               G^GATCC
             1940  GGATCC              
BbeI          187  GGCGCC               GGCGC^C
             1847  GGCGCC              
Bsp1407I      236  TGTACA               T^GTACA
              596  TGTACA              
BspMI        1159  ACCTGC               ACCTGC(4/8)
             1470  GCAGGT              
BsrBI         856  CCGCTC               CCGCTC(-3/-3)
             1824  CCGCTC              
BsrFI         169  ACCGGC               [GA]^CCGG[CT]
             1130  ACCGGC              
BsrGI         236  TGTACA               T^GTACA
              596  TGTACA              
BstUI        1314  CGCG                 CG^CG
             1397  CGCG                
Cfr10I        169  ACCGGC               [GA]^CCGG[CT]
             1130  ACCGGC              
EcoNI         317  CCTTCCTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              777  CCTCAGTAAGG         
HgaI         1435  GACGC                GACGC(5/10)
             1369  GCGTC               
KasI          187  GGCGCC               G^GCGCC
             1847  GGCGCC              
MaeII         227  ACGT                 A^CGT
             1517  ACGT                
MseI          508  TTAA                 T^TAA
              812  TTAA                
MvnI         1314  CGCG                 CG^CG
             1397  CGCG                
NarI          187  GGCGCC               GG^CGCC
             1847  GGCGCC              
PstI         1409  CTGCAG               CTGCA^G
             1468  CTGCAG              
SfoI          187  GGCGCC               GGC^GCC
             1847  GGCGCC              
SmlI          129  CTTGAG               C^T[CT][GA]AG
             1299  CTCGAG              
SspBI         236  TGTACA               T^GTACA
              596  TGTACA              
TfiI          371  GATTC                G^A[AT]TC
             1628  GAATC               
Tru9I         508  TTAA                 T^TAA
              812  TTAA                
Tsp45I        124  GTGAC                ^GT[GC]AC
             1882  GTGAC               
Tsp509I       498  AATT                 ^AATT
              734  AATT                
XmnI          202  GAAACCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1860  GAACATCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   82 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AlwI        
AspHI       AvaI        AvaII       BanI        BanII       
BbvI        BmyI        BsaHI       BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeIII      
MboI        MboII       MflI        MnlI        MslI        
MspA1I      MspI        MvaI        MwoI        NcoI        
NdeII       NlaIII      NlaIV       PleI        PpuMI       
RsaI        SapI        Sau3AI      Sau96I      ScrFI       
SfcI        StyI        TaqI        TseI        TspRI       
TthHB8I     XhoII       
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