Restriction map for pF1KB1292
 (Linear) (Six-base) MAPSORT of: KIBB1292.seq  Check: 311  from: 1  to: 1956



KIBB1292.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:36  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1168    1956 
   Size:       1168     788
 
Acc65I G'GTAC_C

Cuts at:      0     391    1956 
   Size:        391    1565
 
AfeI AGC'GCT

Cuts at:      0    1911    1956 
   Size:       1911      45
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1260    1956 
   Size:       1260     696
 
AleI CACnn'nnGTG

Cuts at:      0    1744    1956 
   Size:       1744     212
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     326    1956 
   Size:        326    1630
 
ApaI G_GGCC'C

Cuts at:      0    1099    1956 
   Size:       1099     857
 
ApaLI G'TGCA_C

Cuts at:      0    1039    1192    1956 
   Size:       1039     153     764

  Fragments arranged by size: 

                1039     764     153
 
ApoI r'AATT_y

Cuts at:      0     497    1956 
   Size:        497    1459
 
AvaI C'yCGr_G

Cuts at:      0     920    1050    1099    1299    1441    1956 
   Size:        920     130      49     200     142     515

  Fragments arranged by size: 

                 920     515     200     142     130      49
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0      92     125    1956 
   Size:         92      33    1831

  Fragments arranged by size: 

                1831      92      33
 
BamHI G'GATC_C

Cuts at:      0    1820    1940    1956 
   Size:       1820     120      16

  Fragments arranged by size: 

                1820     120      16
 
BanI G'GyrC_C

Cuts at:      0     187     391     826     985    1847    1956 
   Size:        187     204     435     159     862     109

  Fragments arranged by size: 

                 862     435     204     187     159     109
 
BanII G_rGCy'C

Cuts at:      0     115    1058    1099    1801    1956 
   Size:        115     943      41     702     155

  Fragments arranged by size: 

                 943     702     155     115      41
 
BbeI G_GCGC'C

Cuts at:      0     191    1851    1956 
   Size:        191    1660     105

  Fragments arranged by size: 

                1660     191     105
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     524    1956 
   Size:        524    1432
 
BbvCI CC'TCA_GC

Cuts at:      0    1731    1956 
   Size:       1731     225
 
BfrBI ATG'CAT

Cuts at:      0     143    1956 
   Size:        143    1813
 
BglII A'GATC_T

Cuts at:      0    1065    1956 
   Size:       1065     891
 
Bme1580I G_kGCm'C

Cuts at:      0     546    1043    1099    1196    1956 
   Size:        546     497      56      97     760

  Fragments arranged by size: 

                 760     546     497      97      56
 
BmgBI CAC'GTC

Cuts at:      0     228    1518    1956 
   Size:        228    1290     438

  Fragments arranged by size: 

                1290     438     228
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     544     799     892    1956 
   Size:        544     255      93    1064

  Fragments arranged by size: 

                1064     544     255      93
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1746    1956 
   Size:       1746     210
 
Bpu10I CC'TnA_GC

Cuts at:      0    1731    1956 
   Size:       1731     225
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     150    1956 
   Size:        150    1806
 
BsaHI Gr'CG_yC

Cuts at:      0     188    1369    1848    1956 
   Size:        188    1181     479     108

  Fragments arranged by size: 

                1181     479     188     108
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     596     626    1005    1035    1956 
   Size:        596      30     379      30     921

  Fragments arranged by size: 

                 921     596     379      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1117    1357    1429    1432    1441    1634    1956 
   Size:       1117     240      72       3       9     193     322

  Fragments arranged by size: 

                1117     322     240     193      72       9       3
 
BseYI C'CCAG_C

Cuts at:      0     265    1897    1956 
   Size:        265    1632      59

  Fragments arranged by size: 

                1632     265      59
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     424    1057    1617    1956 
   Size:        424     633     560     339

  Fragments arranged by size: 

                 633     560     424     339
 
BsiEI CG_ry'CG

Cuts at:      0    1721    1956 
   Size:       1721     235
 
BsiHKAI G_wGCw'C

Cuts at:      0     609     617     942    1043    1196    1956 
   Size:        609       8     325     101     153     760

  Fragments arranged by size: 

                 760     609     325     153     101       8
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     238    1956 
   Size:        238    1718
 
Bsp1286I G_dGCh'C

Cuts at:      0     115     546     609     617     942    1043    1058    1099 
   Size:        115     431      63       8     325     101      15      41

Cuts at:   1099    1196    1801    1956 
   Size:         97     605     155

  Fragments arranged by size: 

                 605     431     325     155     115     101      97      63
                  41      15       8
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1168    1461    1956 
   Size:       1168     293     495

  Fragments arranged by size: 

                1168     495     293
 
BsrBI CCG'CTC

Cuts at:      0     858    1826    1956 
   Size:        858     968     130

  Fragments arranged by size: 

                 968     858     130
 
BsrDI GCAATG_nn'

Cuts at:      0    1518    1956 
   Size:       1518     438
 
BsrFI r'CCGG_y

Cuts at:      0     169    1130    1956 
   Size:        169     961     826

  Fragments arranged by size: 

                 961     826     169
 
BsrGI T'GTAC_A

Cuts at:      0     236     596    1956 
   Size:        236     360    1360

  Fragments arranged by size: 

                1360     360     236
 
BssSI C'ACGA_G

Cuts at:      0     758    1956 
   Size:        758    1198
 
BstEII G'GTnAC_C

Cuts at:      0     123    1956 
   Size:        123    1833
 
BstYI r'GATC_y

Cuts at:      0    1065    1820    1940    1956 
   Size:       1065     755     120      16

  Fragments arranged by size: 

                1065     755     120      16
 
Bsu36I CC'TnA_GG

Cuts at:      0     322    1956 
   Size:        322    1634
 
BtgI C'CryG_G

Cuts at:      0       5     107     281     706     954    1673    1811    1956 
   Size:          5     102     174     425     248     719     138     145

  Fragments arranged by size: 

                 719     425     248     174     145     138     102       5
            
 
BtsI GCAGTG_nn'

Cuts at:      0     345     412    1956 
   Size:        345      67    1544

  Fragments arranged by size: 

                1544     345      67
 
DraI TTT'AAA

Cuts at:      0     813    1956 
   Size:        813    1143
 
DraIII CAC_nnn'GTG

Cuts at:      0    1038    1956 
   Size:       1038     918
 
EaeI y'GGCC_r

Cuts at:      0       2    1002    1379    1808    1956 
   Size:          2    1000     377     429     148

  Fragments arranged by size: 

                1000     429     377     148       2
 
EarI CTCTTCn'nnn_

Cuts at:      0     700     930    1270    1653    1931    1956 
   Size:        700     230     340     383     278      25

  Fragments arranged by size: 

                 700     383     340     278     230      25
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1342    1354    1956 
   Size:       1342      12     602

  Fragments arranged by size: 

                1342     602      12
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     321     781    1956 
   Size:        321     460    1175

  Fragments arranged by size: 

                1175     460     321
 
EcoO109I rG'GnC_Cy

Cuts at:      0     887    1095    1096    1404    1473    1493    1683    1956 
   Size:        887     208       1     308      69      20     190     273

  Fragments arranged by size: 

                 887     308     273     208     190      69      20       1
            
 
HaeII r_GCGC'y

Cuts at:      0     191    1851    1913    1956 
   Size:        191    1660      62      43

  Fragments arranged by size: 

                1660     191      62      43
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1005    1037    1246    1278    1282    1314    1613    1645 
   Size:       1005      32     209      32       4      32     299      32

Cuts at:   1645    1956 
   Size:        311

  Fragments arranged by size: 

                1005     311     299     209      32      32      32      32
                   4
 
KasI G'GCGC_C

Cuts at:      0     187    1847    1956 
   Size:        187    1660     109

  Fragments arranged by size: 

                1660     187     109
 
KpnI G_GTAC'C

Cuts at:      0     395    1956 
   Size:        395    1561
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1004    1352    1605    1781    1956 
   Size:       1004     348     253     176     175

  Fragments arranged by size: 

                1004     348     253     176     175
 
MscI TGG'CCA

Cuts at:      0       4    1956 
   Size:          4    1952
 
MslI CAynn'nnrTG

Cuts at:      0     226    1744    1867    1956 
   Size:        226    1518     123      89

  Fragments arranged by size: 

                1518     226     123      89
 
MspA1I CmG'CkG

Cuts at:      0     295     306    1709    1956 
   Size:        295      11    1403     247

  Fragments arranged by size: 

                1403     295     247      11
 
NarI GG'CG_CC

Cuts at:      0     188    1848    1956 
   Size:        188    1660     108

  Fragments arranged by size: 

                1660     188     108
 
NcoI C'CATG_G

Cuts at:      0       5     281     954    1673    1956 
   Size:          5     276     673     719     283

  Fragments arranged by size: 

                 719     673     283     276       5
 
NdeI CA'TA_TG

Cuts at:      0    1950    1956 
   Size:       1950       6
 
NsiI A_TGCA'T

Cuts at:      0     145    1956 
   Size:        145    1811
 
NspI r_CATG'y

Cuts at:      0     143    1956 
   Size:        143    1813
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1674    1956 
   Size:       1674     282
 
PpuMI rG'GwC_Cy

Cuts at:      0     887    1404    1473    1493    1683    1956 
   Size:        887     517      69      20     190     273

  Fragments arranged by size: 

                 887     517     273     190      69      20
 
PspOMI G'GGCC_C

Cuts at:      0    1095    1956 
   Size:       1095     861
 
PspXI vC'TCGA_Gb

Cuts at:      0    1299    1956 
   Size:       1299     657
 
PstI C_TGCA'G

Cuts at:      0    1413    1472    1956 
   Size:       1413      59     484

  Fragments arranged by size: 

                1413     484      59
 
PvuI CG_AT'CG

Cuts at:      0    1721    1956 
   Size:       1721     235
 
PvuII CAG'CTG

Cuts at:      0     306    1956 
   Size:        306    1650
 
SapI GCTCTTCn'nnn_

Cuts at:      0     700     930    1931    1956 
   Size:        700     230    1001      25

  Fragments arranged by size: 

                1001     700     230      25
 
SbfI CC_TGCA'GG

Cuts at:      0    1472    1956 
   Size:       1472     484
 
SexAI A'CCwGG_T

Cuts at:      0     822    1956 
   Size:        822    1134
 
SfcI C'TryA_G

Cuts at:      0     365    1409    1468    1956 
   Size:        365    1044      59     488

  Fragments arranged by size: 

                1044     488     365      59
 
SfoI GGC'GCC

Cuts at:      0     189    1849    1956 
   Size:        189    1660     107

  Fragments arranged by size: 

                1660     189     107
 
SmlI C'TyrA_G

Cuts at:      0     129    1299    1956 
   Size:        129    1170     657

  Fragments arranged by size: 

                1170     657     129
 
SphI G_CATG'C

Cuts at:      0     143    1956 
   Size:        143    1813
 
SspI AAT'ATT

Cuts at:      0     158    1956 
   Size:        158    1798
 
StuI AGG'CCT

Cuts at:      0    1640    1956 
   Size:       1640     316
 
StyI C'CwwG_G

Cuts at:      0       5     281     830     954    1673    1956 
   Size:          5     276     549     124     719     283

  Fragments arranged by size: 

                 719     549     283     276     124       5
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1522    1956 
   Size:       1522     434
 
TatI w'GTAC_w

Cuts at:      0     236     486     596    1956 
   Size:        236     250     110    1360

  Fragments arranged by size: 

                1360     250     236     110
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     596     628    1956 
   Size:        596      32    1328

  Fragments arranged by size: 

                1328     596      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1536    1956 
   Size:       1536     420
 
XhoI C'TCGA_G

Cuts at:      0    1299    1956 
   Size:       1299     657
 
XmnI GAAnn'nnTTC

Cuts at:      0     206    1864    1956 
   Size:        206    1658      92

  Fragments arranged by size: 

                1658     206      92
 

 Enzymes that do cut:

     AarI   Acc65I     AfeI     AhdI     AleI     AlfI     ApaI    ApaLI
     ApoI     AvaI     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI    BfrBI    BglII Bme1580I    BmgBI     BmrI     BplI   Bpu10I
    BpuEI    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI
   BstEII    BstYI   Bsu36I     BtgI     BtsI     DraI   DraIII     EaeI
     EarI     EciI    EcoNI EcoO109I    HaeII    Hin4I     KasI     KpnI
     MmeI     MscI     MslI   MspA1I     NarI     NcoI     NdeI     NsiI
     NspI    PflMI    PpuMI   PspOMI    PspXI     PstI     PvuI    PvuII
     SapI     SbfI    SexAI     SfcI     SfoI     SmlI     SphI     SspI
     StuI     StyI    TaqII     TatI     TstI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AcuI    AflII   AflIII     AgeI     AloI
    AlwNI     AscI     AseI    AsiSI    AvrII     BcgI    BciVI     BclI
     BglI     BlpI     BmtI     BpmI     BsaI    BsaAI    BsaBI    BsaWI
    BsiWI     BsmI    BspEI    BspHI   BssHII   BstAPI    BstBI    BstXI
  BstZ17I    BtgZI     ClaI    CspCI     DrdI     EagI  EcoICRI  Eco57MI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII
     HpaI     MfeI     MluI     NaeI   NgoMIV     NheI     NotI     NruI
     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI     PsrI    RsrII     SacI    SacII     SalI    SanDI     ScaI
     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SwaI
  Tth111I     XbaI     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com