Restriction map for pF1KB0039
##################################
# NO CUT ENZYMES (  120 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AcyI        
AflII       AflIII      AgeI        AhdI        Aor51HI     
ApaI        AscI        AseI        AsnI        Asp700I     
AspEI       AviII       AvrII       BalI        BamHI       
BbeI        BbrPI       BcgI        BfrI        BglI        
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsiEI       
BsiWI       BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrFI       BssHII      BssSI       Bst1107I    
BstEII      BstPI       BstUI       BstZ17I     CelII       
Cfr10I      ClaI        CpoI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoO65I     
EcoRV       FseI        FspI        HaeII       HgaI        
Hin1I       HindIII     HpaI        KasI        KspI        
MamI        MfeI        MluI        MluNI       MroI        
MscI        MspA1I      MunI        MvnI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NspI        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SbfI        ScaI        SfiI        
SfoI        SgrAI       SmaI        SmlI        SnaBI       
SphI        Sse8387I    SspI        SwaI        XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

Acc65I        916  GGTACC               G^GTACC
AclI         1508  AACGTT               AA^CGTT
Alw44I        432  GTGCAC               G^TGCAC
AlwI           42  GGATC                GGATC(4/5)
AocI          222  CCTAAGG              CC^T[ACGT]AGG
ApaLI         432  GTGCAC               G^TGCAC
Asp718I       916  GGTACC               G^GTACC
AspI          156  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         1167  CTCGGG               C^[CT]CG[GA]G
BanII        1025  GAGCCC               G[GA]GC[CT]^C
BbsI          394  GAAGAC               GAAGAC(2/6)
BbvCI         948  CCTCAGC              CCTCAGC(-5/-2)
BciVI        1172  GTATCC               GTATCC(6/5)
BclI         1317  TGATCA               T^GATCA
BcnI         1133  CCGGG                CC^[GC]GG
BglII         114  AGATCT               A^GATCT
BpuAI         394  GAAGAC               GAAGAC(2/6)
BseRI         795  CTCCTC               GAGGAG(10/8)
BsmAI          70  GTCTC                GTCTC(1/5)
BsmBI          69  CGTCTC               CGTCTC(1/5)
BsoBI        1167  CTCGGG               C^[CT]CG[GA]G
Bsp1407I     1208  TGTACA               T^GTACA
BsrGI        1208  TGTACA               T^GTACA
BstYI         114  AGATCT               [GA]^GATC[CT]
Bsu36I        222  CCTAAGG              CC^T[ACGT]AGG
DraI         1092  TTTAAA               TTT^AAA
DraIII        908  CACCAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          445  GACATCCGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        222  CCTAAGG              CC^T[ACGT]AGG
EcoRI          57  GAATTC               G^AATTC
EcoT22I       343  ATGCAT               ATGCA^T
FbaI         1317  TGATCA               T^GATCA
HincII       1003  GTCAAC               GT[CT]^[GA]AC
HindII       1003  GTCAAC               GT[CT]^[GA]AC
KpnI          916  GGTACC               GGTAC^C
MflI          114  AGATCT               [GA]^GATC[CT]
MslI          710  CATCTGTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI         1133  CCGGG                CC^[GC]GG
NsiI          343  ATGCAT               ATGCA^T
PflFI         156  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         828  CCACCCAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          452  GAGTC                GAGTC(4/5)
Psp1406I     1508  AACGTT               AA^CGTT
SapI          313  GCTCTTC              GCTCTTC(1/4)
SexAI         157  ACCTGGT              A^CC[AT]GGT
SpeI          961  ACTAGT               A^CTAGT
SspBI        1208  TGTACA               T^GTACA
StuI         1160  AGGCCT               AGG^CCT
TfiI         1218  GAATC                G^A[AT]TC
Tsp45I        535  GTGAC                ^GT[GC]AC
Tth111I       156  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        828  CCACCCAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         114  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AfaI          917  GTAC                 GT^AC
             1209  GTAC                
AlwNI         707  CAGCATCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1321  CAGGACCTG           
BpmI          679  CTCCAG               CTGGAG(16/14)
             1043  CTGGAG              
BstBI         187  TTCGAA               TT^CGAA
             1512  TTCGAA              
BstXI         151  CCAAGGACCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1305  CCAGGAGTTTGG        
CfoI          704  GCGC                 GCG^C
             1195  GCGC                
EcoNI        1152  CCTCACTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1299  CCTGCCCCAGG         
HapII         489  CCGG                 C^CGG
             1133  CCGG                
HhaI          704  GCGC                 GCG^C
             1195  GCGC                
HinP1I        704  GCGC                 G^CGC
             1195  GCGC                
HinfI         452  GAGTC                G^A[ACGT]TC
             1218  GAATC               
HpaII         489  CCGG                 C^CGG
             1133  CCGG                
MaeII         218  ACGT                 A^CGT
             1509  ACGT                
MspI          489  CCGG                 C^CGG
             1133  CCGG                
NspV          187  TTCGAA               TT^CGAA
             1512  TTCGAA              
RsaI          917  GTAC                 GT^AC
             1209  GTAC                
SfuI          187  TTCGAA               TT^CGAA
             1512  TTCGAA              
TaqI          188  TCGA                 T^CGA
             1513  TCGA                
TthHB8I       188  TCGA                 T^CGA
             1513  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   66 enzymes) #
#########################################

AciI        AcsI        AluI        ApoI        AspHI       
AvaII       BanI        BbvI        BfaI        BmyI        
BsaJI       BsgI        BsiHKAI     BsiYI       BslI        
BsmFI       BsmI        Bsp1286I    BspMI       BsrDI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
Cac8I       Cfr13I      DdeI        DpnII       DraII       
DsaI        EarI        EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HphI        
ItaI        Ksp632I     MaeI        MaeIII      MboI        
MboII       MnlI        MseI        MvaI        MwoI        
NcoI        NdeII       NlaIII      NlaIV       PpuMI       
PstI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StyI        Tru9I       TseI        Tsp509I     
TspRI       
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