Restriction map for pF1KB0016
 (Linear) (Six-base) MAPSORT of: KIBB0016.seq  Check: 7571  from: 1  to: 5070



KIBB0016.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:36  ..

AatII G_ACGT'C

Cuts at:      0    1788    5070 
   Size:       1788    3282
 
AccI GT'mk_AC

Cuts at:      0    1081    4868    5070 
   Size:       1081    3787     202

  Fragments arranged by size: 

                3787    1081     202
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     334     700     727    1072    1763    1949    2701    3682 
   Size:        334     366      27     345     691     186     752     981

Cuts at:   3682    3743    4448    4487    5070 
   Size:         61     705      39     583

  Fragments arranged by size: 

                 981     752     705     691     583     366     345     334
                 186      61      39      27
 
AflII C'TTAA_G

Cuts at:      0    3184    5070 
   Size:       3184    1886
 
AflIII A'CryG_T

Cuts at:      0     657    2044    2065    4166    5070 
   Size:        657    1387      21    2101     904

  Fragments arranged by size: 

                2101    1387     904     657      21
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    4857    5070 
   Size:       4857     213
 
AlwNI CAG_nnn'CTG

Cuts at:      0     228     679    2854    3219    4033    5070 
   Size:        228     451    2175     365     814    1037

  Fragments arranged by size: 

                2175    1037     814     451     365     228
 
ApaI G_GGCC'C

Cuts at:      0     737    5070 
   Size:        737    4333
 
ApoI r'AATT_y

Cuts at:      0     149     286     491    1177    1329    3192    3484    3625 
   Size:        149     137     205     686     152    1863     292     141

Cuts at:   3625    5070 
   Size:       1445

  Fragments arranged by size: 

                1863    1445     686     292     205     152     149     141
                 137
 
AseI AT'TA_AT

Cuts at:      0    1376    5070 
   Size:       1376    3694
 
AvaI C'yCGr_G

Cuts at:      0    1043    1567    3002    3863    5070 
   Size:       1043     524    1435     861    1207

  Fragments arranged by size: 

                1435    1207    1043     861     524
 
BamHI G'GATC_C

Cuts at:      0    3598    5070 
   Size:       3598    1472
 
BanI G'GyrC_C

Cuts at:      0    1817    2796    4275    5070 
   Size:       1817     979    1479     795

  Fragments arranged by size: 

                1817    1479     979     795
 
BanII G_rGCy'C

Cuts at:      0     737    2434    2644    2843    3226    3536    4270    5070 
   Size:        737    1697     210     199     383     310     734     800

  Fragments arranged by size: 

                1697     800     737     734     383     310     210     199
            
 
BbeI G_GCGC'C

Cuts at:      0    1821    5070 
   Size:       1821    3249
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     670     798    2174    2497    4046    4436    5070 
   Size:        670     128    1376     323    1549     390     634

  Fragments arranged by size: 

                1549    1376     670     634     390     323     128
 
BbvCI CC'TCA_GC

Cuts at:      0    2309    5070 
   Size:       2309    2761
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    3702    5070 
   Size:       3702    1368
 
BfrBI ATG'CAT

Cuts at:      0    3423    5070 
   Size:       3423    1647
 
BglII A'GATC_T

Cuts at:      0     558    4356    5070 
   Size:        558    3798     714

  Fragments arranged by size: 

                3798     714     558
 
BlpI GC'TnA_GC

Cuts at:      0      97    2393    3220    5070 
   Size:         97    2296     827    1850

  Fragments arranged by size: 

                2296    1850     827      97
 
Bme1580I G_kGCm'C

Cuts at:      0      58     737     992    2122    2208    4280    5070 
   Size:         58     679     255    1130      86    2072     790

  Fragments arranged by size: 

                2072    1130     790     679     255      86      58
 
BmgBI CAC'GTC

Cuts at:      0     658    5070 
   Size:        658    4412
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     470    1497    3179    4881    5070 
   Size:        470    1027    1682    1702     189

  Fragments arranged by size: 

                1702    1682    1027     470     189
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    4244    5070 
   Size:       4244     826
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     701    1023    1482    1561    2605    3340    3358    4280 
   Size:        701     322     459      79    1044     735      18     922

Cuts at:   4280    4324    4337    4916    5070 
   Size:         44      13     579     154

  Fragments arranged by size: 

                1044     922     735     701     579     459     322     154
                  79      44      18      13
 
Bpu10I CC'TnA_GC

Cuts at:      0    2081    2309    2579    2876    4322    5070 
   Size:       2081     228     270     297    1446     748

  Fragments arranged by size: 

                2081    1446     748     297     270     228
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     259    3088    4214    5070 
   Size:        259    2829    1126     856

  Fragments arranged by size: 

                2829    1126     856     259
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     470    2368    3656    5056    5070 
   Size:        470    1898    1288    1400      14

  Fragments arranged by size: 

                1898    1400    1288     470      14
 
BsaBI GATnn'nnATC

Cuts at:      0    4138    5070 
   Size:       4138     932
 
BsaHI Gr'CG_yC

Cuts at:      0    1785    1809    1818    3090    5070 
   Size:       1785      24       9    1272    1980

  Fragments arranged by size: 

                1980    1785    1272      24       9
 
BsaWI w'CCGG_w

Cuts at:      0    1266    5070 
   Size:       1266    3804
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2825    2855    5070 
   Size:       2825      30    2215

  Fragments arranged by size: 

                2825    2215      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      94    1230    1558    3983    4165    4816    5070 
   Size:         94    1136     328    2425     182     651     254

  Fragments arranged by size: 

                2425    1136     651     328     254     182      94
 
BseYI C'CCAG_C

Cuts at:      0     730    1583    5070 
   Size:        730     853    3487

  Fragments arranged by size: 

                3487     853     730
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     562    3911    4211    4643    5070 
   Size:        562    3349     300     432     427

  Fragments arranged by size: 

                3349     562     432     427     300
 
BsiEI CG_ry'CG

Cuts at:      0     111     157    5070 
   Size:        111      46    4913

  Fragments arranged by size: 

                4913     111      46
 
BsiHKAI G_wGCw'C

Cuts at:      0    2580    2843    3536    4738    5070 
   Size:       2580     263     693    1202     332

  Fragments arranged by size: 

                2580    1202     693     332     263
 
BsiWI C'GTAC_G

Cuts at:      0    3711    5070 
   Size:       3711    1359
 
BsmI GAATG_Cn'

Cuts at:      0    1879    3423    4824    5070 
   Size:       1879    1544    1401     246

  Fragments arranged by size: 

                1879    1544    1401     246
 
Bsp1286I G_dGCh'C

Cuts at:      0      58     737     992    2122    2208    2434    2580    2644 
   Size:         58     679     255    1130      86     226     146      64

Cuts at:   2644    2843    3226    3536    4270    4280    4738    5070 
   Size:        199     383     310     734      10     458     332

  Fragments arranged by size: 

                1130     734     679     458     383     332     310     255
                 226     199     146      86      64      58      10
 
BspHI T'CATG_A

Cuts at:      0    1616    1922    2568    2762    5070 
   Size:       1616     306     646     194    2308

  Fragments arranged by size: 

                2308    1616     646     306     194
 
BsrBI CCG'CTC

Cuts at:      0      96    2019    2085    5070 
   Size:         96    1923      66    2985

  Fragments arranged by size: 

                2985    1923      96      66
 
BsrDI GCAATG_nn'

Cuts at:      0     251     761    2828    5070 
   Size:        251     510    2067    2242

  Fragments arranged by size: 

                2242    2067     510     251
 
BsrFI r'CCGG_y

Cuts at:      0     136    5070 
   Size:        136    4934
 
BsrGI T'GTAC_A

Cuts at:      0    1655    1959    5070 
   Size:       1655     304    3111

  Fragments arranged by size: 

                3111    1655     304
 
BssSI C'ACGA_G

Cuts at:      0    1700    1837    5070 
   Size:       1700     137    3233

  Fragments arranged by size: 

                3233    1700     137
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1647    2251    4198    5070 
   Size:       1647     604    1947     872

  Fragments arranged by size: 

                1947    1647     872     604
 
BstBI TT'CG_AA

Cuts at:      0    2343    5070 
   Size:       2343    2727
 
BstEII G'GTnAC_C

Cuts at:      0     396     783    4158    5070 
   Size:        396     387    3375     912

  Fragments arranged by size: 

                3375     912     396     387
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     744    1505    3378    5070 
   Size:        744     761    1873    1692

  Fragments arranged by size: 

                1873    1692     761     744
 
BstYI r'GATC_y

Cuts at:      0      90     304     317     345     558    1866    2736    3309 
   Size:         90     214      13      28     213    1308     870     573

Cuts at:   3309    3598    4356    5052    5070 
   Size:        289     758     696      18

  Fragments arranged by size: 

                1308     870     758     696     573     289     214     213
                  90      28      18      13
 
BstZ17I GTA'TAC

Cuts at:      0    1082    5070 
   Size:       1082    3988
 
Bsu36I CC'TnA_GG

Cuts at:      0     581    1813    5070 
   Size:        581    1232    3257

  Fragments arranged by size: 

                3257    1232     581
 
BtgI C'CryG_G

Cuts at:      0    1405    1898    3371    5070 
   Size:       1405     493    1473    1699

  Fragments arranged by size: 

                1699    1473    1405     493
 
BtsI GCAGTG_nn'

Cuts at:      0    2029    3115    3745    3984    5070 
   Size:       2029    1086     630     239    1086

  Fragments arranged by size: 

                2029    1086    1086     630     239
 
DraIII CAC_nnn'GTG

Cuts at:      0    2910    5070 
   Size:       2910    2160
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2224    3678    5070 
   Size:       2224    1454    1392

  Fragments arranged by size: 

                2224    1454    1392
 
EaeI y'GGCC_r

Cuts at:      0     108     154    3818    4652    5070 
   Size:        108      46    3664     834     418

  Fragments arranged by size: 

                3664     834     418     108      46
 
EagI C'GGCC_G

Cuts at:      0     108     154    5070 
   Size:        108      46    4916

  Fragments arranged by size: 

                4916     108      46
 
EarI CTCTTCn'nnn_

Cuts at:      0     640    1298    1437    2555    3575    4124    4258    4463 
   Size:        640     658     139    1118    1020     549     134     205

Cuts at:   4463    4506    4670    5070 
   Size:         43     164     400

  Fragments arranged by size: 

                1118    1020     658     640     549     400     205     164
                 139     134      43
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      46     142    1296    3189    4413    5070 
   Size:         46      96    1154    1893    1224     657

  Fragments arranged by size: 

                1893    1224    1154     657      96      46
 
EcoICRI GAG'CTC

Cuts at:      0    2841    3534    5070 
   Size:       2841     693    1536

  Fragments arranged by size: 

                2841    1536     693
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     334     700     701     727    1023    1072    1482    1561 
   Size:        334     366       1      26     296      49     410      79

Cuts at:   1561    1763    1949    2605    2701    3340    3358    3682    3743 
   Size:        202     186     656      96     639      18     324      61

Cuts at:   3743    4280    4324    4337    4448    4487    4916    5070 
   Size:        537      44      13     111      39     429     154

  Fragments arranged by size: 

                 656     639     537     429     410     366     334     324
                 296     202     186     154     111      96      79      61
                  49      44      39      26      18      13       1
 
EcoO109I rG'GnC_Cy

Cuts at:      0    3732    4850    5070 
   Size:       3732    1118     220

  Fragments arranged by size: 

                3732    1118     220
 
EcoRI G'AATT_C

Cuts at:      0     491    3192    5070 
   Size:        491    2701    1878

  Fragments arranged by size: 

                2701    1878     491
 
EcoRV GAT'ATC

Cuts at:      0     442    1236    2152    5070 
   Size:        442     794     916    2918

  Fragments arranged by size: 

                2918     916     794     442
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    4738    5070 
   Size:       4738     332
 
FspI TGC'GCA

Cuts at:      0    4830    5070 
   Size:       4830     240
 
HaeII r_GCGC'y

Cuts at:      0     228    1821    5070 
   Size:        228    1593    3249

  Fragments arranged by size: 

                3249    1593     228
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1220    1225    1252    1257    1683    1715    2626    2658 
   Size:       1220       5      27       5     426      32     911      32

Cuts at:   2658    3061    3093    3790    3822    4759    4791    5070 
   Size:        403      32     697      32     937      32     279

  Fragments arranged by size: 

                1220     937     911     697     426     403     279      32
                  32      32      32      32      27       5       5
 
HincII GTy'rAC

Cuts at:      0     864    5070 
   Size:        864    4206
 
HindIII A'AGCT_T

Cuts at:      0     198    5070 
   Size:        198    4872
 
HpaI GTT'AAC

Cuts at:      0     864    5070 
   Size:        864    4206
 
KasI G'GCGC_C

Cuts at:      0    1817    5070 
   Size:       1817    3253
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1306    2131    2514    2690    4332    5070 
   Size:       1306     825     383     176    1642     738

  Fragments arranged by size: 

                1642    1306     825     738     383     176
 
MscI TGG'CCA

Cuts at:      0    3820    4654    5070 
   Size:       3820     834     416

  Fragments arranged by size: 

                3820     834     416
 
MslI CAynn'nnrTG

Cuts at:      0     628     832    1376    2573    3153    3376    5070 
   Size:        628     204     544    1197     580     223    1694

  Fragments arranged by size: 

                1694    1197     628     580     544     223     204
 
MspA1I CmG'CkG

Cuts at:      0    2397    3560    3605    3910    5070 
   Size:       2397    1163      45     305    1160

  Fragments arranged by size: 

                2397    1163    1160     305      45
 
NarI GG'CG_CC

Cuts at:      0    1818    5070 
   Size:       1818    3252
 
NcoI C'CATG_G

Cuts at:      0    1898    3371    5070 
   Size:       1898    1473    1699

  Fragments arranged by size: 

                1898    1699    1473
 
NdeI CA'TA_TG

Cuts at:      0     504    1148    4056    4393    5070 
   Size:        504     644    2908     337     677

  Fragments arranged by size: 

                2908     677     644     504     337
 
NsiI A_TGCA'T

Cuts at:      0    3425    5070 
   Size:       3425    1645
 
NspI r_CATG'y

Cuts at:      0     831    1344    2036    2048    2069    4170    4201    5070 
   Size:        831     513     692      12      21    2101      31     869

  Fragments arranged by size: 

                2101     869     831     692     513      31      21      12
            
 
PciI A'CATG_T

Cuts at:      0    2044    2065    4166    5070 
   Size:       2044      21    2101     904

  Fragments arranged by size: 

                2101    2044     904      21
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     167    2440    2811    3846    5070 
   Size:        167    2273     371    1035    1224

  Fragments arranged by size: 

                2273    1224    1035     371     167
 
PfoI T'CCnGG_A

Cuts at:      0     341    1349    4361    5070 
   Size:        341    1008    3012     709

  Fragments arranged by size: 

                3012    1008     709     341
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     450     482    5070 
   Size:        450      32    4588

  Fragments arranged by size: 

                4588     450      32
 
PspOMI G'GGCC_C

Cuts at:      0     733    5070 
   Size:        733    4337
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1055    1087    5070 
   Size:       1055      32    3983

  Fragments arranged by size: 

                3983    1055      32
 
PstI C_TGCA'G

Cuts at:      0    1533    3740    4710    5070 
   Size:       1533    2207     970     360

  Fragments arranged by size: 

                2207    1533     970     360
 
PvuII CAG'CTG

Cuts at:      0    2397    3560    3605    3910    5070 
   Size:       2397    1163      45     305    1160

  Fragments arranged by size: 

                2397    1163    1160     305      45
 
RsrII CG'GwC_CG

Cuts at:      0    3230    5070 
   Size:       3230    1840
 
SacI G_AGCT'C

Cuts at:      0    2843    3536    5070 
   Size:       2843     693    1534

  Fragments arranged by size: 

                2843    1534     693
 
SapI GCTCTTCn'nnn_

Cuts at:      0    4258    5070 
   Size:       4258     812
 
SfcI C'TryA_G

Cuts at:      0    1529    2833    2887    3523    3736    4706    5070 
   Size:       1529    1304      54     636     213     970     364

  Fragments arranged by size: 

                1529    1304     970     636     364     213      54
 
SfoI GGC'GCC

Cuts at:      0    1819    5070 
   Size:       1819    3251
 
SmlI C'TyrA_G

Cuts at:      0     238    1043    3002    3067    3184    3863    4229    5070 
   Size:        238     805    1959      65     117     679     366     841

  Fragments arranged by size: 

                1959     841     805     679     366     238     117      65
            
 
SpeI A'CTAG_T

Cuts at:      0    1164    5070 
   Size:       1164    3906
 
SphI G_CATG'C

Cuts at:      0    2036    4201    5070 
   Size:       2036    2165     869

  Fragments arranged by size: 

                2165    2036     869
 
StuI AGG'CCT

Cuts at:      0    2482    5070 
   Size:       2482    2588
 
StyI C'CwwG_G

Cuts at:      0     161     737    1898    3371    4246    4571    5070 
   Size:        161     576    1161    1473     875     325     499

  Fragments arranged by size: 

                1473    1161     875     576     499     325     161
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     526    1957    2913    3247    4433    4912    5070 
   Size:        526    1431     956     334    1186     479     158

  Fragments arranged by size: 

                1431    1186     956     526     479     334     158
 
TatI w'GTAC_w

Cuts at:      0    1655    1759    1959    5070 
   Size:       1655     104     200    3111

  Fragments arranged by size: 

                3111    1655     200     104
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1253    1285    5070 
   Size:       1253      32    3785

  Fragments arranged by size: 

                3785    1253      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     669    5070 
   Size:        669    4401
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     258    1632    2463    3456    5070 
   Size:        258    1374     831     993    1614

  Fragments arranged by size: 

                1614    1374     993     831     258
 
XhoI C'TCGA_G

Cuts at:      0    1043    3002    3863    5070 
   Size:       1043    1959     861    1207

  Fragments arranged by size: 

                1959    1207    1043     861
 
XmnI GAAnn'nnTTC

Cuts at:      0     491    2003    2564    3029    4043    4133    5070 
   Size:        491    1512     561     465    1014      90     937

  Fragments arranged by size: 

                1512    1014     937     561     491     465      90
 
ZraI GAC'GTC

Cuts at:      0    1786    5070 
   Size:       1786    3284
 

 Enzymes that do cut:

    AatII     AccI     AcuI    AflII   AflIII     AlfI    AlwNI     ApaI
     ApoI     AseI     AvaI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI    BciVI    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
 Bsp1286I    BspHI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI     BtsI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     MmeI     MscI     MslI   MspA1I     NarI
     NcoI     NdeI     NsiI     NspI     PciI    PflMI     PfoI     PpiI
   PspOMI     PsrI     PstI    PvuII    RsrII     SacI     SapI     SfcI
     SfoI     SmlI     SpeI     SphI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XcmI     XhoI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AfeI     AgeI     AhdI     AleI     AloI
    ApaLI     AscI    AsiSI    AvrII     BaeI     BcgI     BclI     BglI
     BmtI    BsaAI    BsmBI    BspEI    BspMI   BssHII    BtgZI     ClaI
    CspCI     DraI    EcoNI     FseI    FspAI     KpnI     MfeI     MluI
     NaeI   NgoMIV     NheI     NotI     NruI     PacI     PasI     PmeI
     PmlI    PpuMI    PshAI     PsiI    PspXI     PvuI    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI
    SnaBI     SrfI     SspI     SwaI     XbaI     XmaI
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