Restriction map for pF1KB0011
 (Linear) (Six-base) MAPSORT of: KIBB0011.seq  Check: 500  from: 1  to: 1440



KIBB0011.orf

 With 182 enzymes: SgfI * 

                        September 18, 2009 17:00  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1185    1440 
   Size:       1185     255
 
AflII C'TTAA_G

Cuts at:      0    1222    1440 
   Size:       1222     218
 
AhdI GACnn_n'nnGTC

Cuts at:      0      49    1440 
   Size:         49    1391
 
AlwNI CAG_nnn'CTG

Cuts at:      0     160     294     313    1440 
   Size:        160     134      19    1127

  Fragments arranged by size: 

                1127     160     134      19
 
ApaI G_GGCC'C

Cuts at:      0     462     583     599     647     845    1440 
   Size:        462     121      16      48     198     595

  Fragments arranged by size: 

                 595     462     198     121      48      16
 
AvaI C'yCGr_G

Cuts at:      0      21      30      54      75     302     413     632     701 
   Size:         21       9      24      21     227     111     219      69

Cuts at:    701     877    1440 
   Size:        176     563

  Fragments arranged by size: 

                 563     227     219     176     111      69      24      21
                  21       9
 
AvrII C'CTAG_G

Cuts at:      0     714    1440 
   Size:        714     726
 
BanI G'GyrC_C

Cuts at:      0     186     814    1440 
   Size:        186     628     626

  Fragments arranged by size: 

                 628     626     186
 
BanII G_rGCy'C

Cuts at:      0     153     462     583     599     639     647     658     845 
   Size:        153     309     121      16      40       8      11     187

Cuts at:    845     890    1028    1345    1440 
   Size:         45     138     317      95

  Fragments arranged by size: 

                 317     309     187     153     138     121      95      45
                  40      16      11       8
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1043    1440 
   Size:       1043     397
 
BglI GCCn_nnn'nGGC

Cuts at:      0     368     805     981    1440 
   Size:        368     437     176     459

  Fragments arranged by size: 

                 459     437     368     176
 
Bme1580I G_kGCm'C

Cuts at:      0     191     462     583     599     647     819     845    1440 
   Size:        191     271     121      16      48     172      26     595

  Fragments arranged by size: 

                 595     271     191     172     121      48      26      16
            
 
BmgBI CAC'GTC

Cuts at:      0    1395    1440 
   Size:       1395      45
 
BmtI G_CTAG'C

Cuts at:      0     788    1440 
   Size:        788     652
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     695    1440 
   Size:        695     745
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     137     316     623     796    1141    1440 
   Size:        137     179     307     173     345     299

  Fragments arranged by size: 

                 345     307     299     179     173     137
 
Bpu10I CC'TnA_GC

Cuts at:      0     884    1440 
   Size:        884     556
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1033    1440 
   Size:       1033     407
 
BsaAI yAC'GTr

Cuts at:      0     492    1305    1440 
   Size:        492     813     135

  Fragments arranged by size: 

                 813     492     135
 
BsaWI w'CCGG_w

Cuts at:      0    1142    1382    1440 
   Size:       1142     240      58

  Fragments arranged by size: 

                1142     240      58
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      32      62    1440 
   Size:         32      30    1378

  Fragments arranged by size: 

                1378      32      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     510     793    1440 
   Size:        510     283     647

  Fragments arranged by size: 

                 647     510     283
 
BseYI C'CCAG_C

Cuts at:      0       4     231     651     708     769     819     844    1100 
   Size:          4     227     420      57      61      50      25     256

Cuts at:   1100    1111    1294    1428    1440 
   Size:         11     183     134      12

  Fragments arranged by size: 

                 420     256     227     183     134      61      57      50
                  25      12      11       4
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      56     650    1440 
   Size:         56     594     790

  Fragments arranged by size: 

                 790     594      56
 
BsmI GAATG_Cn'

Cuts at:      0     938    1076    1440 
   Size:        938     138     364

  Fragments arranged by size: 

                 938     364     138
 
Bsp1286I G_dGCh'C

Cuts at:      0     153     191     462     583     599     639     647     658 
   Size:        153      38     271     121      16      40       8      11

Cuts at:    658     819     845     890    1028    1345    1440 
   Size:        161      26      45     138     317      95

  Fragments arranged by size: 

                 317     271     161     153     138     121      95      45
                  40      38      26      16      11       8
 
BspEI T'CCGG_A

Cuts at:      0    1142    1440 
   Size:       1142     298
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     216     270    1440 
   Size:        216      54    1170

  Fragments arranged by size: 

                1170     216      54
 
BsrBI CCG'CTC

Cuts at:      0     514    1440 
   Size:        514     926
 
BsrDI GCAATG_nn'

Cuts at:      0     871    1173    1440 
   Size:        871     302     267

  Fragments arranged by size: 

                 871     302     267
 
BsrFI r'CCGG_y

Cuts at:      0     532    1440 
   Size:        532     908
 
BssHII G'CGCG_C

Cuts at:      0    1308    1440 
   Size:       1308     132
 
BssSI C'ACGA_G

Cuts at:      0     574    1440 
   Size:        574     866
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     313     814    1440 
   Size:        313     501     626

  Fragments arranged by size: 

                 626     501     313
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1119    1440 
   Size:       1119     321
 
BstYI r'GATC_y

Cuts at:      0    1145    1158    1440 
   Size:       1145      13     282

  Fragments arranged by size: 

                1145     282      13
 
BtgI C'CryG_G

Cuts at:      0      47     200     335     659     800     915    1440 
   Size:         47     153     135     324     141     115     525

  Fragments arranged by size: 

                 525     324     153     141     135     115      47
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     235    1440 
   Size:        235    1205
 
BtsI GCAGTG_nn'

Cuts at:      0     758    1440 
   Size:        758     682
 
EaeI y'GGCC_r

Cuts at:      0     797     863     982    1440 
   Size:        797      66     119     458

  Fragments arranged by size: 

                 797     458     119      66
 
EarI CTCTTCn'nnn_

Cuts at:      0     680     740    1440 
   Size:        680      60     700

  Fragments arranged by size: 

                 700     680      60
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     130     238    1440 
   Size:        130     108    1202

  Fragments arranged by size: 

                1202     130     108
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     137     316     623     796    1141    1185    1440 
   Size:        137     179     307     173     345      44     255

  Fragments arranged by size: 

                 345     307     255     179     173     137      44
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     356    1440 
   Size:        356    1084
 
EcoO109I rG'GnC_Cy

Cuts at:      0      43      58     228     361     458     459     570     580 
   Size:         43      15     170     133      97       1     111      10

Cuts at:    580     596     643     644     674    1017    1107    1290    1440 
   Size:         16      47       1      30     343      90     183     150

  Fragments arranged by size: 

                 343     183     170     150     133     111      97      90
                  47      43      30      16      15      10       1       1
            
 
FspI TGC'GCA

Cuts at:      0     937    1440 
   Size:        937     503
 
HaeII r_GCGC'y

Cuts at:      0      68    1440 
   Size:         68    1372
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      35      67    1440 
   Size:         35      32    1373

  Fragments arranged by size: 

                1373      35      32
 
HindIII A'AGCT_T

Cuts at:      0    1357    1440 
   Size:       1357      83
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     884    1440 
   Size:        884     556
 
MscI TGG'CCA

Cuts at:      0     865    1440 
   Size:        865     575
 
MspA1I CmG'CkG

Cuts at:      0     235     310     442     651     823     855     917     933 
   Size:        235      75     132     209     172      32      62      16

Cuts at:    933    1087    1100    1440 
   Size:        154      13     340

  Fragments arranged by size: 

                 340     235     209     172     154     132      75      62
                  32      16      13
 
NaeI GCC'GGC

Cuts at:      0     534    1440 
   Size:        534     906
 
NcoI C'CATG_G

Cuts at:      0     335     659    1440 
   Size:        335     324     781

  Fragments arranged by size: 

                 781     335     324
 
NgoMIV G'CCGG_C

Cuts at:      0     532    1440 
   Size:        532     908
 
NheI G'CTAG_C

Cuts at:      0     784    1440 
   Size:        784     656
 
NruI TCG'CGA

Cuts at:      0     262    1440 
   Size:        262    1178
 
NspI r_CATG'y

Cuts at:      0    1374    1440 
   Size:       1374      66
 
PasI CC'CwG_GG

Cuts at:      0    1029    1440 
   Size:       1029     411
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     160    1440 
   Size:        160    1280
 
PmlI CAC'GTG

Cuts at:      0     492    1440 
   Size:        492     948
 
PpuMI rG'GwC_Cy

Cuts at:      0      43      58     228    1017    1107    1290    1440 
   Size:         43      15     170     789      90     183     150

  Fragments arranged by size: 

                 789     183     170     150      90      43      15
 
PspOMI G'GGCC_C

Cuts at:      0     458     579     595     643     841    1440 
   Size:        458     121      16      48     198     599

  Fragments arranged by size: 

                 599     458     198     121      48      16
 
PstI C_TGCA'G

Cuts at:      0     318     528     834    1440 
   Size:        318     210     306     606

  Fragments arranged by size: 

                 606     318     306     210
 
PvuII CAG'CTG

Cuts at:      0     310     442     823    1100    1440 
   Size:        310     132     381     277     340

  Fragments arranged by size: 

                 381     340     310     277     132
 
SacII CC_GC'GG

Cuts at:      0     918    1440 
   Size:        918     522
 
SapI GCTCTTCn'nnn_

Cuts at:      0     680    1440 
   Size:        680     760
 
SfcI C'TryA_G

Cuts at:      0     314     496     524     830    1440 
   Size:        314     182      28     306     610

  Fragments arranged by size: 

                 610     314     306     182      28
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     981    1440 
   Size:        981     459
 
SmaI CCC'GGG

Cuts at:      0      23      32      56      77    1440 
   Size:         23       9      24      21    1363

  Fragments arranged by size: 

                1363      24      23      21       9
 
SmlI C'TyrA_G

Cuts at:      0     413    1012    1222    1440 
   Size:        413     599     210     218

  Fragments arranged by size: 

                 599     413     218     210
 
StuI AGG'CCT

Cuts at:      0     719    1001    1440 
   Size:        719     282     439

  Fragments arranged by size: 

                 719     439     282
 
StyI C'CwwG_G

Cuts at:      0     335     659     714    1229    1262    1440 
   Size:        335     324      55     515      33     178

  Fragments arranged by size: 

                 515     335     324     178      55      33
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     331     624    1021    1440 
   Size:        331     293     397     419

  Fragments arranged by size: 

                 419     397     331     293
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      32      64     312     344    1137    1169    1440 
   Size:         32      32     248      32     793      32     271

  Fragments arranged by size: 

                 793     271     248      32      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1396    1440 
   Size:       1396      44
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     852    1440 
   Size:        852     588
 
XhoI C'TCGA_G

Cuts at:      0     413    1440 
   Size:        413    1027
 
XmaI C'CCGG_G

Cuts at:      0      21      30      54      75    1440 
   Size:         21       9      24      21    1365

  Fragments arranged by size: 

                1365      24      21      21       9
 

 Enzymes that do cut:

     AcuI    AflII     AhdI    AlwNI     ApaI     AvaI    AvrII     BanI
    BanII     BbsI     BglI Bme1580I    BmgBI     BmtI     BplI     BpmI
   Bpu10I    BpuEI    BsaAI    BsaWI    BsaXI    BseRI    BseYI     BsgI
     BsmI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI   BssHII
    BssSI   BstAPI    BstXI    BstYI     BtgI    BtgZI     BtsI     EaeI
     EarI     EciI  Eco57MI    EcoNI EcoO109I     FspI    HaeII    Hin4I
  HindIII     MmeI     MscI   MspA1I     NaeI     NcoI   NgoMIV     NheI
     NruI     NspI     PasI    PflMI     PmlI    PpuMI   PspOMI     PstI
    PvuII    SacII     SapI     SfcI     SfiI     SmaI     SmlI     StuI
     StyI    TaqII     TstI  Tth111I     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AleI     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI
     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI
    BglII     BlpI     BmrI     BsaI    BsaBI    BsaHI    BsiEI  BsiHKAI
    BsiWI    BsmBI    BspHI    BsrGI    BstBI   BstEII  BstZ17I   Bsu36I
     ClaI    CspCI     DraI   DraIII     DrdI     EagI  EcoICRI    EcoRI
    EcoRV     FalI     FseI    FspAI   HincII     HpaI     KasI     KpnI
     MfeI     MluI     MslI     NarI     NdeI     NotI     NsiI     PacI
     PciI     PfoI     PmeI     PpiI    PshAI     PsiI    PspXI     PsrI
     PvuI    RsrII     SacI     SalI    SanDI     SbfI     ScaI    SexAI
     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI     TatI     XbaI     XmnI     ZraI
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