Restriction map for
pF1KA2004
(Linear) (Six-base) MAPSORT of: KIAA2004.seq Check: 6245 from: 1 to: 4767
KIAA2004.orf
With 182 enzymes: SgfI *
July 18, 2007 13:36 ..
AccI GT'mk_AC
Cuts at: 0 540 3377 4767
Size: 540 2837 1390
Fragments arranged by size:
2837 1390 540
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 653 1312 4767
Size: 653 659 3455
Fragments arranged by size:
3455 659 653
AflIII A'CryG_T
Cuts at: 0 478 4767
Size: 478 4289
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 861 3267 4448 4767
Size: 861 2406 1181 319
Fragments arranged by size:
2406 1181 861 319
ApaLI G'TGCA_C
Cuts at: 0 1732 4767
Size: 1732 3035
ApoI r'AATT_y
Cuts at: 0 101 502 586 640 681 1012 1135 1799
Size: 101 401 84 54 41 331 123 664
Cuts at: 1799 2011 2020 2269 2818 2863 3042 3076 3626
Size: 212 9 249 549 45 179 34 550
Cuts at: 3626 3781 4158 4383 4767
Size: 155 377 225 384
Fragments arranged by size:
664 550 549 401 384 377 331 249
225 212 179 155 123 101 84 54
45 41 34 9
AseI AT'TA_AT
Cuts at: 0 1780 4523 4767
Size: 1780 2743 244
Fragments arranged by size:
2743 1780 244
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 467 500 4767
Size: 467 33 4267
Fragments arranged by size:
4267 467 33
BamHI G'GATC_C
Cuts at: 0 2537 3720 4767
Size: 2537 1183 1047
Fragments arranged by size:
2537 1183 1047
BanI G'GyrC_C
Cuts at: 0 2881 4767
Size: 2881 1886
BanII G_rGCy'C
Cuts at: 0 425 4040 4767
Size: 425 3615 727
Fragments arranged by size:
3615 727 425
BbsI GAAGACnn'nnnn_
Cuts at: 0 824 1598 2048 2876 4516 4767
Size: 824 774 450 828 1640 251
Fragments arranged by size:
1640 828 824 774 450 251
BciVI GTATCCnnnnn_n'
Cuts at: 0 3232 3554 4201 4767
Size: 3232 322 647 566
Fragments arranged by size:
3232 647 566 322
BclI T'GATC_A
Cuts at: 0 2657 4767
Size: 2657 2110
BfrBI ATG'CAT
Cuts at: 0 3244 4413 4767
Size: 3244 1169 354
Fragments arranged by size:
3244 1169 354
BglII A'GATC_T
Cuts at: 0 1761 4767
Size: 1761 3006
Bme1580I G_kGCm'C
Cuts at: 0 1736 2884 4767
Size: 1736 1148 1883
Fragments arranged by size:
1883 1736 1148
BmgBI CAC'GTC
Cuts at: 0 120 4767
Size: 120 4647
BmrI ACTGGGnnnn_n'
Cuts at: 0 266 3321 3376 4767
Size: 266 3055 55 1391
Fragments arranged by size:
3055 1391 266 55
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1681 4767
Size: 1681 3086
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3616 4188 4767
Size: 3616 572 579
Fragments arranged by size:
3616 579 572
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3462 4355 4767
Size: 3462 893 412
Fragments arranged by size:
3462 893 412
BsaAI yAC'GTr
Cuts at: 0 688 2373 4767
Size: 688 1685 2394
Fragments arranged by size:
2394 1685 688
BsaBI GATnn'nnATC
Cuts at: 0 56 4767
Size: 56 4711
BsaWI w'CCGG_w
Cuts at: 0 2702 4767
Size: 2702 2065
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2312 2342 4767
Size: 2312 30 2425
Fragments arranged by size:
2425 2312 30
BseYI C'CCAG_C
Cuts at: 0 1465 4767
Size: 1465 3302
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2183 4767
Size: 2183 2584
BsiHKAI G_wGCw'C
Cuts at: 0 425 1736 4767
Size: 425 1311 3031
Fragments arranged by size:
3031 1311 425
BsmI GAATG_Cn'
Cuts at: 0 848 949 3244 3484 4767
Size: 848 101 2295 240 1283
Fragments arranged by size:
2295 1283 848 240 101
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1426 4767
Size: 1426 3341
Bsp1286I G_dGCh'C
Cuts at: 0 425 1736 2884 4040 4767
Size: 425 1311 1148 1156 727
Fragments arranged by size:
1311 1156 1148 727 425
BspEI T'CCGG_A
Cuts at: 0 2702 4767
Size: 2702 2065
BspHI T'CATG_A
Cuts at: 0 1943 2660 4767
Size: 1943 717 2107
Fragments arranged by size:
2107 1943 717
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1478 4767
Size: 1478 3289
BsrDI GCAATG_nn'
Cuts at: 0 386 654 1488 2153 2360 3306 4767
Size: 386 268 834 665 207 946 1461
Fragments arranged by size:
1461 946 834 665 386 268 207
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3090 3472 4767
Size: 3090 382 1295
Fragments arranged by size:
3090 1295 382
BstEII G'GTnAC_C
Cuts at: 0 2328 4767
Size: 2328 2439
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1535 4767
Size: 1535 3232
BstYI r'GATC_y
Cuts at: 0 1659 1695 1761 2537 3720 4767
Size: 1659 36 66 776 1183 1047
Fragments arranged by size:
1659 1183 1047 776 66 36
BtgI C'CryG_G
Cuts at: 0 729 4767
Size: 729 4038
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3134 4767
Size: 3134 1633
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 848 883 4767
Size: 848 35 3884
Fragments arranged by size:
3884 848 35
DraI TTT'AAA
Cuts at: 0 1086 1098 4016 4767
Size: 1086 12 2918 751
Fragments arranged by size:
2918 1086 751 12
DraIII CAC_nnn'GTG
Cuts at: 0 2812 4767
Size: 2812 1955
DrdI GACnn_nn'nnGTC
Cuts at: 0 826 4767
Size: 826 3941
EaeI y'GGCC_r
Cuts at: 0 4169 4318 4767
Size: 4169 149 449
Fragments arranged by size:
4169 449 149
EarI CTCTTCn'nnn_
Cuts at: 0 617 1829 2094 2771 3857 4767
Size: 617 1212 265 677 1086 910
Fragments arranged by size:
1212 1086 910 677 617 265
EcoICRI GAG'CTC
Cuts at: 0 423 4767
Size: 423 4344
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 653 1312 3616 4188 4767
Size: 653 659 2304 572 579
Fragments arranged by size:
2304 659 653 579 572
EcoNI CCTnn'n_nnAGG
Cuts at: 0 551 4767
Size: 551 4216
EcoRI G'AATT_C
Cuts at: 0 502 586 681 2020 4767
Size: 502 84 95 1339 2747
Fragments arranged by size:
2747 1339 502 95 84
EcoRV GAT'ATC
Cuts at: 0 1941 4767
Size: 1941 2826
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 175 1783 2120 3309 3628 4258 4767
Size: 175 1608 337 1189 319 630 509
Fragments arranged by size:
1608 1189 630 509 337 319 175
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1185 1217 1318 1350 1428 1460 2930 2962
Size: 1185 32 101 32 78 32 1470 32
Cuts at: 2962 3668 3700 4693 4725 4767
Size: 706 32 993 32 42
Fragments arranged by size:
1470 1185 993 706 101 78 42 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 4678 4767
Size: 4678 89
HindIII A'AGCT_T
Cuts at: 0 1358 4767
Size: 1358 3409
MfeI C'AATT_G
Cuts at: 0 4430 4608 4767
Size: 4430 178 159
Fragments arranged by size:
4430 178 159
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 509 855 3005 4218 4767
Size: 509 346 2150 1213 549
Fragments arranged by size:
2150 1213 549 509 346
MscI TGG'CCA
Cuts at: 0 4171 4320 4767
Size: 4171 149 447
Fragments arranged by size:
4171 447 149
MslI CAynn'nnrTG
Cuts at: 0 1793 3119 3131 3179 4767
Size: 1793 1326 12 48 1588
Fragments arranged by size:
1793 1588 1326 48 12
MspA1I CmG'CkG
Cuts at: 0 1469 4767
Size: 1469 3298
NcoI C'CATG_G
Cuts at: 0 729 4767
Size: 729 4038
NdeI CA'TA_TG
Cuts at: 0 2778 4409 4767
Size: 2778 1631 358
Fragments arranged by size:
2778 1631 358
NsiI A_TGCA'T
Cuts at: 0 3246 4415 4767
Size: 3246 1169 352
Fragments arranged by size:
3246 1169 352
NspI r_CATG'y
Cuts at: 0 482 3475 4767
Size: 482 2993 1292
Fragments arranged by size:
2993 1292 482
PciI A'CATG_T
Cuts at: 0 478 4767
Size: 478 4289
PflMI CCAn_nnn'nTGG
Cuts at: 0 1903 2221 3261 4767
Size: 1903 318 1040 1506
Fragments arranged by size:
1903 1506 1040 318
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3031 3063 4767
Size: 3031 32 1704
Fragments arranged by size:
3031 1704 32
PstI C_TGCA'G
Cuts at: 0 2432 4767
Size: 2432 2335
PvuII CAG'CTG
Cuts at: 0 1469 4767
Size: 1469 3298
SacI G_AGCT'C
Cuts at: 0 425 4767
Size: 425 4342
SapI GCTCTTCn'nnn_
Cuts at: 0 2771 4767
Size: 2771 1996
ScaI AGT'ACT
Cuts at: 0 891 2434 4767
Size: 891 1543 2333
Fragments arranged by size:
2333 1543 891
SfcI C'TryA_G
Cuts at: 0 541 2428 2450 4767
Size: 541 1887 22 2317
Fragments arranged by size:
2317 1887 541 22
SmlI C'TyrA_G
Cuts at: 0 3477 4370 4767
Size: 3477 893 397
Fragments arranged by size:
3477 893 397
SnaBI TAC'GTA
Cuts at: 0 2373 4767
Size: 2373 2394
SspI AAT'ATT
Cuts at: 0 2727 3849 4767
Size: 2727 1122 918
Fragments arranged by size:
2727 1122 918
StyI C'CwwG_G
Cuts at: 0 729 767 2234 2735 4767
Size: 729 38 1467 501 2032
Fragments arranged by size:
2032 1467 729 501 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1277 1751 4767
Size: 1277 474 3016
Fragments arranged by size:
3016 1277 474
TatI w'GTAC_w
Cuts at: 0 385 889 1235 2379 2432 2462 4767
Size: 385 504 346 1144 53 30 2305
Fragments arranged by size:
2305 1144 504 385 346 53 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2919 2951 4767
Size: 2919 32 1816
Fragments arranged by size:
2919 1816 32
XmnI GAAnn'nnTTC
Cuts at: 0 2269 2728 2845 3046 4767
Size: 2269 459 117 201 1721
Fragments arranged by size:
2269 1721 459 201 117
Enzymes that do cut:
AccI AcuI AflIII AlfI ApaLI ApoI AseI BaeI
BamHI BanI BanII BbsI BciVI BclI BfrBI BglII
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaAI BsaBI
BsaWI BsaXI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrDI BstAPI BstEII BstXI BstYI
BtgI BtgZI CspCI DraI DraIII DrdI EaeI EarI
EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI Hin4I HincII
HindIII MfeI MmeI MscI MslI MspA1I NcoI NdeI
NsiI NspI PciI PflMI PpiI PstI PvuII SacI
SapI ScaI SfcI SmlI SnaBI SspI StyI TaqII
TatI TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI AlwNI ApaI AscI AsiSI AvaI AvrII
BbeI BbvCI BcgI BglI BlpI BmtI Bpu10I BsaI
BsaHI BseRI BsiEI BsiWI BsrBI BsrFI BsrGI BssHII
BssSI BstBI BstZ17I Bsu36I BtsI ClaI EagI EciI
EcoO109I FseI FspI FspAI HaeII HpaI KasI KpnI
MluI NaeI NarI NgoMIV NheI NotI NruI PacI
PasI PfoI PmeI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SpeI SphI
SrfI StuI SwaI Tth111I XbaI XcmI XhoI XmaI
ZraI