Restriction map for pF1KA1964
##################################
# NO CUT ENZYMES (   99 enzymes) #
##################################

AatII       AccI        AclI        AcsI        AflII       
AgeI        AhdI        Alw44I      AocI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
AspEI       AvrII       BbsI        BcgI        BciVI       
BclI        BfrI        BlnI        BlpI        Bpu1102I    
BpuAI       BsaBI       BsaI        BsiWI       BsmBI       
Bsp1407I    BspDI       BsrDI       BsrGI       BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstXI       
BstZ17I     Bsu36I      CelII       ClaI        DraI        
DraIII      DrdI        Eam1105I    Eco81I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
HindIII     HpaI        KspI        MamI        MfeI        
MluI        MseI        MunI        NaeI        NdeI        
NgoMIV      NheI        NruI        NsiI        NspV        
PacI        PaeR7I      PflMI       PinAI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        SacII       
SalI        SbfI        SexAI       SfiI        SfuI        
SgrAI       SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        Tru9I       
Tsp509I     Van91I      XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

Acc65I       2282  GGTACC               G^GTACC
AccIII       2178  TCCGGA               T^CCGGA
ApaI         1087  GGGCCC               GGGCC^C
Asp718I      2282  GGTACC               G^GTACC
AspI         1225  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        2063  TGCGCA               TGC^GCA
BamHI         557  GGATCC               G^GATCC
BglI          859  GCCACACTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseAI        2178  TCCGGA               T^CCGGA
BsmI         2025  GAATGC               GAATGC(1/-1)
BspEI        2178  TCCGGA               T^CCGGA
BspHI         947  TCATGA               T^CATGA
BspLU11I      659  ACATGT               A^CATGT
CpoI          347  CGGTCCG              CG^G[AT]CCG
FspI         2063  TGCGCA               TGC^GCA
HincII        640  GTCAAC               GT[CT]^[GA]AC
HindII        640  GTCAAC               GT[CT]^[GA]AC
KpnI         2282  GGTACC               GGTAC^C
MroI         2178  TCCGGA               T^CCGGA
NotI            8  GCGGCCGC             GC^GGCCGC
PflFI        1225  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
RcaI          947  TCATGA               T^CATGA
RsrII         347  CGGTCCG              CG^G[AT]CCG
SacI          898  GAGCTC               GAGCT^C
SapI         2337  GCTCTTC              GCTCTTC(1/4)
ScaI          785  AGTACT               AGT^ACT
SmaI          136  CCCGGG               CCC^GGG
StuI         1103  AGGCCT               AGG^CCT
TfiI         1212  GATTC                G^A[AT]TC
Tth111I      1225  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI          711  TCTAGA               T^CTAGA
XcmI         2231  CCAATGACTTTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         136  CCCGGG               C^CCGGG
XmaI          136  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

Aor51HI       482  AGCGCT               AGC^GCT
             2360  AGCGCT              
AspHI         898  GAGCTC               G[AT]GC[AT]^C
             2119  GTGCTC              
BalI         1191  TGGCCA               TGG^CCA
             1559  TGGCCA              
BbrPI         231  CACGTG               CAC^GTG
              996  CACGTG              
BbvCI        1671  CCTCAGC              CCTCAGC(-5/-2)
              469  GCTGAGG             
BglII         773  AGATCT               A^GATCT
             1568  AGATCT              
BsaWI        2178  TCCGGA               [AT]^CCGG[AT]
             2195  TCCGGT              
BsiHKAI       898  GAGCTC               G[AT]GC[AT]^C
             2119  GTGCTC              
BsmAI         907  GAGAC                GTCTC(1/5)
             2107  GAGAC               
BspMI        1917  ACCTGC               ACCTGC(4/8)
             2297  ACCTGC              
BsrFI          84  GCCGGT               [GA]^CCGG[CT]
              254  ACCGGC              
BstAPI       2089  GCAGACTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2151  GCAGACCCTGC         
Cfr10I         84  GCCGGT               [GA]^CCGG[CT]
              254  ACCGGC              
EagI            9  CGGCCG               C^GGCCG
              757  CGGCCG              
EclXI           9  CGGCCG               C^GGCCG
              757  CGGCCG              
Eco47III      482  AGCGCT               AGC^GCT
             2360  AGCGCT              
Eco52I          9  CGGCCG               C^GGCCG
              757  CGGCCG              
MluNI        1191  TGGCCA               TGG^CCA
             1559  TGGCCA              
MscI         1191  TGGCCA               TGG^CCA
             1559  TGGCCA              
NcoI         1322  CCATGG               C^CATGG
             2076  CCATGG              
NspI          659  ACATGT               [GA]CATG^[CT]
             1355  ACATGC              
PmaCI         231  CACGTG               CAC^GTG
              996  CACGTG              
PmlI          231  CACGTG               CAC^GTG
              996  CACGTG              
PpuMI        1427  GGGTCCT              [GA]G^G[AT]CC[CT]
             1965  AGGACCT             


#########################################
# 3 OR MORE CUT ENZYMES (  101 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AflIII      
AluI        AlwI        AlwNI       AvaI        AvaII       
BanI        BanII       BbeI        BbvI        BcnI        
BfaI        BmyI        BpmI        BsaAI       BsaHI       
BsaJI       BseRI       BsgI        BsiEI       BsiYI       
BslI        BsmFI       BsoBI       Bsp1286I    BsrBI       
BsrI        BssHII      BssKI       BstF5I      BstNI       
BstUI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        KasI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NarI        
NciI        NdeII       NlaIII      NlaIV       PleI        
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SfoI        StyI        
TaqI        TseI        Tsp45I      TspRI       TthHB8I     
XhoII       
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