Restriction map for pF1KA1964
 (Linear) (Six-base) MAPSORT of: KIAA1964.seq  Check: 9806  from: 1  to: 2367



KIAA1964.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:36  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2306    2367 
   Size:       2306      61
 
Acc65I G'GTAC_C

Cuts at:      0    2282    2367 
   Size:       2282      85
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     172     772    1438    2367 
   Size:        172     600     666     929

  Fragments arranged by size: 

                 929     666     600     172
 
AfeI AGC'GCT

Cuts at:      0     484    2362    2367 
   Size:        484    1878       5

  Fragments arranged by size: 

                1878     484       5
 
AflIII A'CryG_T

Cuts at:      0     659     995     997    2367 
   Size:        659     336       2    1370

  Fragments arranged by size: 

                1370     659     336       2
 
AleI CACnn'nnGTG

Cuts at:      0     998    1000    2367 
   Size:        998       2    1367

  Fragments arranged by size: 

                1367     998       2
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1334    2011    2367 
   Size:       1334     677     356

  Fragments arranged by size: 

                1334     677     356
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1374    1998    2013    2157    2367 
   Size:       1374     624      15     144     210

  Fragments arranged by size: 

                1374     624     210     144      15
 
ApaI G_GGCC'C

Cuts at:      0    1091    2367 
   Size:       1091    1276
 
AvaI C'yCGr_G

Cuts at:      0      37     136     789    1390    1812    1954    2367 
   Size:         37      99     653     601     422     142     413

  Fragments arranged by size: 

                 653     601     422     413     142      99      37
 
BamHI G'GATC_C

Cuts at:      0     557    2367 
   Size:        557    1810
 
BanI G'GyrC_C

Cuts at:      0      81     124     391     459     856    2082    2282    2367 
   Size:         81      43     267      68     397    1226     200      85

  Fragments arranged by size: 

                1226     397     267     200      85      81      68      43
            
 
BanII G_rGCy'C

Cuts at:      0      47     205     902    1091    1178    1482    2178    2367 
   Size:         47     158     697     189      87     304     696     189

  Fragments arranged by size: 

                 697     696     304     189     189     158      87      47
            
 
BbeI G_GCGC'C

Cuts at:      0      85     463     860    2367 
   Size:         85     378     397    1507

  Fragments arranged by size: 

                1507     397     378      85
 
BbvCI CC'TCA_GC

Cuts at:      0     470    1672    2367 
   Size:        470    1202     695

  Fragments arranged by size: 

                1202     695     470
 
BglI GCCn_nnn'nGGC

Cuts at:      0     865    2367 
   Size:        865    1502
 
BglII A'GATC_T

Cuts at:      0     773    1568    2367 
   Size:        773     795     799

  Fragments arranged by size: 

                 799     795     773
 
Bme1580I G_kGCm'C

Cuts at:      0     816    1091    2087    2100    2367 
   Size:        816     275     996      13     267

  Fragments arranged by size: 

                 996     816     275     267      13
 
BmgBI CAC'GTC

Cuts at:      0     183    1227    2367 
   Size:        183    1044    1140

  Fragments arranged by size: 

                1140    1044     183
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      36     787     847     856    1309    1327    2367 
   Size:         36     751      60       9     453      18    1040

  Fragments arranged by size: 

                1040     751     453      60      36      18       9
 
Bpu10I CC'TnA_GC

Cuts at:      0     274     470     818    1672    2367 
   Size:        274     196     348     854     695

  Fragments arranged by size: 

                 854     695     348     274     196
 
BsaAI yAC'GTr

Cuts at:      0     233     998    2118    2367 
   Size:        233     765    1120     249

  Fragments arranged by size: 

                1120     765     249     233
 
BsaHI Gr'CG_yC

Cuts at:      0      82     460     524     857    1846    2219    2367 
   Size:         82     378      64     333     989     373     148

  Fragments arranged by size: 

                 989     378     373     333     148      82      64
 
BsaWI w'CCGG_w

Cuts at:      0    2178    2195    2367 
   Size:       2178      17     172

  Fragments arranged by size: 

                2178     172      17
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     235     265    2209    2239    2367 
   Size:        235      30    1944      30     128

  Fragments arranged by size: 

                1944     235     128      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      55     664     745     784     865    1408    1510    1513 
   Size:         55     609      81      39      81     543     102       3

Cuts at:   1513    1516    1519    1531    1543    2367 
   Size:          3       3      12      12     824

  Fragments arranged by size: 

                 824     609     543     102      81      81      55      39
                  12      12       3       3       3
 
BseYI C'CCAG_C

Cuts at:      0     485     875    1090    1154    1761    2144    2367 
   Size:        485     390     215      64     607     383     223

  Fragments arranged by size: 

                 607     485     390     383     223     215      64
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     352     556    1323    1612    1988    2367 
   Size:        352     204     767     289     376     379

  Fragments arranged by size: 

                 767     379     376     352     289     204
 
BsiEI CG_ry'CG

Cuts at:      0      12      89     708     760    2367 
   Size:         12      77     619      52    1607

  Fragments arranged by size: 

                1607     619      77      52      12
 
BsiHKAI G_wGCw'C

Cuts at:      0     902    2123    2367 
   Size:        902    1221     244

  Fragments arranged by size: 

                1221     902     244
 
BsmI GAATG_Cn'

Cuts at:      0    2031    2367 
   Size:       2031     336
 
Bsp1286I G_dGCh'C

Cuts at:      0      47     205     816     902    1091    1178    1482    2087 
   Size:         47     158     611      86     189      87     304     605

Cuts at:   2087    2100    2123    2178    2367 
   Size:         13      23      55     189

  Fragments arranged by size: 

                 611     605     304     189     189     158      87      86
                  55      47      23      13
 
BspEI T'CCGG_A

Cuts at:      0    2178    2367 
   Size:       2178     189
 
BspHI T'CATG_A

Cuts at:      0     947    2367 
   Size:        947    1420
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1926    2306    2367 
   Size:       1926     380      61

  Fragments arranged by size: 

                1926     380      61
 
BsrBI CCG'CTC

Cuts at:      0     224     246     543    2367 
   Size:        224      22     297    1824

  Fragments arranged by size: 

                1824     297     224      22
 
BsrFI r'CCGG_y

Cuts at:      0      84     254    2367 
   Size:         84     170    2113

  Fragments arranged by size: 

                2113     170      84
 
BssHII G'CGCG_C

Cuts at:      0     186     406     512     514    2367 
   Size:        186     220     106       2    1853

  Fragments arranged by size: 

                1853     220     186     106       2
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2095    2157    2367 
   Size:       2095      62     210

  Fragments arranged by size: 

                2095     210      62
 
BstYI r'GATC_y

Cuts at:      0     218     557     773    1568    2072    2367 
   Size:        218     339     216     795     504     295

  Fragments arranged by size: 

                 795     504     339     295     218     216
 
BtgI C'CryG_G

Cuts at:      0     464    1322    2076    2367 
   Size:        464     858     754     291

  Fragments arranged by size: 

                 858     754     464     291
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     296     405    2367 
   Size:        296     109    1962

  Fragments arranged by size: 

                1962     296     109
 
BtsI GCAGTG_nn'

Cuts at:      0    1296    1903    2254    2367 
   Size:       1296     607     351     113

  Fragments arranged by size: 

                1296     607     351     113
 
EaeI y'GGCC_r

Cuts at:      0       9      53     757    1191    1473    1559    2367 
   Size:          9      44     704     434     282      86     808

  Fragments arranged by size: 

                 808     704     434     282      86      44       9
 
EagI C'GGCC_G

Cuts at:      0       9     757    2367 
   Size:          9     748    1610

  Fragments arranged by size: 

                1610     748       9
 
EarI CTCTTCn'nnn_

Cuts at:      0     682    1050    1222    1520    2344    2367 
   Size:        682     368     172     298     824      23

  Fragments arranged by size: 

                 824     682     368     298     172      23
 
EcoICRI GAG'CTC

Cuts at:      0     900    2367 
   Size:        900    1467
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      36     172     772     787     847     856    1309    1327 
   Size:         36     136     600      15      60       9     453      18

Cuts at:   1327    1438    2367 
   Size:        111     929

  Fragments arranged by size: 

                 929     600     453     136     111      60      36      18
                  15       9
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     169     679    1110    1435    2367 
   Size:        169     510     431     325     932

  Fragments arranged by size: 

                 932     510     431     325     169
 
EcoO109I rG'GnC_Cy

Cuts at:      0     372    1087    1115    1428    1966    2315    2367 
   Size:        372     715      28     313     538     349      52

  Fragments arranged by size: 

                 715     538     372     349     313      52      28
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     624    1233    2367 
   Size:        624     609    1134

  Fragments arranged by size: 

                1134     624     609
 
FspI TGC'GCA

Cuts at:      0    2065    2367 
   Size:       2065     302
 
FspAI rTGC'GCAy

Cuts at:      0    2065    2367 
   Size:       2065     302
 
HaeII r_GCGC'y

Cuts at:      0      85     151     384     463     486     860    1375    1881 
   Size:         85      66     233      79      23     374     515     506

Cuts at:   1881    2364    2367 
   Size:        483       3

  Fragments arranged by size: 

                 515     506     483     374     233      85      79      66
                  23       3
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     765     797    1387    1419    2209    2241    2302    2334 
   Size:        765      32     590      32     790      32      61      32

Cuts at:   2334    2367 
   Size:         33

  Fragments arranged by size: 

                 790     765     590      61      33      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     642    2367 
   Size:        642    1725
 
KasI G'GCGC_C

Cuts at:      0      81     459     856    2367 
   Size:         81     378     397    1511

  Fragments arranged by size: 

                1511     397     378      81
 
KpnI G_GTAC'C

Cuts at:      0    2286    2367 
   Size:       2286      81
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     611     722    1466    2367 
   Size:        611     111     744     901

  Fragments arranged by size: 

                 901     744     611     111
 
MscI TGG'CCA

Cuts at:      0    1193    1561    2367 
   Size:       1193     368     806

  Fragments arranged by size: 

                1193     806     368
 
MslI CAynn'nnrTG

Cuts at:      0     998    1000    1360    1993    2367 
   Size:        998       2     360     633     374

  Fragments arranged by size: 

                 998     633     374     360       2
 
MspA1I CmG'CkG

Cuts at:      0      14     291    1136    1416    1456    1743    1761    1824 
   Size:         14     277     845     280      40     287      18      63

Cuts at:   1824    2013    2144    2169    2367 
   Size:        189     131      25     198

  Fragments arranged by size: 

                 845     287     280     277     198     189     131      63
                  40      25      18      14
 
NarI GG'CG_CC

Cuts at:      0      82     460     857    2367 
   Size:         82     378     397    1510

  Fragments arranged by size: 

                1510     397     378      82
 
NcoI C'CATG_G

Cuts at:      0    1322    2076    2367 
   Size:       1322     754     291

  Fragments arranged by size: 

                1322     754     291
 
NotI GC'GGCC_GC

Cuts at:      0       9    2367 
   Size:          9    2358
 
NspI r_CATG'y

Cuts at:      0     663    1359    2367 
   Size:        663     696    1008

  Fragments arranged by size: 

                1008     696     663
 
PasI CC'CwG_GG

Cuts at:      0    1125    2367 
   Size:       1125    1242
 
PciI A'CATG_T

Cuts at:      0     659    2367 
   Size:        659    1708
 
PfoI T'CCnGG_A

Cuts at:      0     833    1007    1220    2367 
   Size:        833     174     213    1147

  Fragments arranged by size: 

                1147     833     213     174
 
PmlI CAC'GTG

Cuts at:      0     233     998    2367 
   Size:        233     765    1369

  Fragments arranged by size: 

                1369     765     233
 
PpuMI rG'GwC_Cy

Cuts at:      0    1428    1966    2367 
   Size:       1428     538     401

  Fragments arranged by size: 

                1428     538     401
 
PspOMI G'GGCC_C

Cuts at:      0    1087    2367 
   Size:       1087    1280
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     646     678    2367 
   Size:        646      32    1689

  Fragments arranged by size: 

                1689     646      32
 
PstI C_TGCA'G

Cuts at:      0     263    1545    2162    2367 
   Size:        263    1282     617     205

  Fragments arranged by size: 

                1282     617     263     205
 
PvuII CAG'CTG

Cuts at:      0    1416    1743    1824    2013    2169    2367 
   Size:       1416     327      81     189     156     198

  Fragments arranged by size: 

                1416     327     198     189     156      81
 
RsrII CG'GwC_CG

Cuts at:      0     348    2367 
   Size:        348    2019
 
SacI G_AGCT'C

Cuts at:      0     902    2367 
   Size:        902    1465
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2344    2367 
   Size:       2344      23
 
ScaI AGT'ACT

Cuts at:      0     787    2367 
   Size:        787    1580
 
SfcI C'TryA_G

Cuts at:      0     259    1541    1788    2158    2367 
   Size:        259    1282     247     370     209

  Fragments arranged by size: 

                1282     370     259     247     209
 
SfoI GGC'GCC

Cuts at:      0      83     461     858    2367 
   Size:         83     378     397    1509

  Fragments arranged by size: 

                1509     397     378      83
 
SmaI CCC'GGG

Cuts at:      0     138    2367 
   Size:        138    2229
 
SrfI GCCC'GGGC

Cuts at:      0     138    2367 
   Size:        138    2229
 
StuI AGG'CCT

Cuts at:      0    1105    2367 
   Size:       1105    1262
 
StyI C'CwwG_G

Cuts at:      0     980    1322    2076    2306    2367 
   Size:        980     342     754     230      61

  Fragments arranged by size: 

                 980     754     342     230      61
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     477    1006    2367 
   Size:        477     529    1361

  Fragments arranged by size: 

                1361     529     477
 
TatI w'GTAC_w

Cuts at:      0     785    1598    2367 
   Size:        785     813     769

  Fragments arranged by size: 

                 813     785     769
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2287    2319    2367 
   Size:       2287      32      48

  Fragments arranged by size: 

                2287      48      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1228    2367 
   Size:       1228    1139
 
XbaI T'CTAG_A

Cuts at:      0     711    2367 
   Size:        711    1656
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2238    2367 
   Size:       2238     129
 
XmaI C'CCGG_G

Cuts at:      0     136    2367 
   Size:        136    2231
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI     AfeI   AflIII     AleI     AlfI    AlwNI
     ApaI     AvaI    BamHI     BanI    BanII     BbeI    BbvCI     BglI
    BglII Bme1580I    BmgBI     BpmI   Bpu10I    BsaAI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrFI   BssHII   BstAPI    BstYI
     BtgI    BtgZI     BtsI     EaeI     EagI     EarI  EcoICRI  Eco57MI
    EcoNI EcoO109I     FalI     FspI    FspAI    HaeII    Hin4I   HincII
     KasI     KpnI     MmeI     MscI     MslI   MspA1I     NarI     NcoI
     NotI     NspI     PasI     PciI     PfoI     PmlI    PpuMI   PspOMI
     PsrI     PstI    PvuII    RsrII     SacI     SapI     ScaI     SfcI
     SfoI     SmaI     SrfI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AccI     AclI    AflII     AgeI     AhdI     AloI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI     BbsI     BcgI
    BciVI     BclI    BfrBI     BlpI     BmrI     BmtI     BplI    BpuEI
     BsaI    BsaBI    BsiWI    BsmBI    BsrDI    BsrGI    BssSI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI     DraI   DraIII
     DrdI     EciI    EcoRI    EcoRV     FseI  HindIII     HpaI     MfeI
     MluI     NaeI     NdeI   NgoMIV     NheI     NruI     NsiI     PacI
    PflMI     PmeI     PpiI    PshAI     PsiI    PspXI     PvuI    SacII
     SalI    SanDI     SbfI    SexAI     SfiI     SgfI    SgrAI     SmlI
    SnaBI     SpeI     SphI     SspI     SwaI     XhoI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com