(Circular) (Six-base) MAPSORT of: pF1KB7824.seq Check: 333 from: 1 to: 4925 pF1KB7824 4925 bp With 182 enzymes: SgfI * October 7, 2009 14:24 .. AccI GT'mk_AC Cuts at: 1012 1655 1963 1012 Size: 643 308 3974 Fragments arranged by size: 3974 643 308 Acc65I G'GTAC_C Cuts at: 1941 1941 Size: 4925 AclI AA'CG_TT Cuts at: 4765 4765 Size: 4925 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 588 713 990 1242 2750 3182 3929 4197 588 Size: 125 277 252 1508 432 747 268 1316 Fragments arranged by size: 1508 1316 747 432 277 268 252 125 AflIII A'CryG_T Cuts at: 2277 3381 4358 2277 Size: 1104 977 2844 Fragments arranged by size: 2844 1104 977 AgeI A'CCGG_T Cuts at: 3313 3313 Size: 4925 AlwNI CAG_nnn'CTG Cuts at: 736 2230 3797 736 Size: 1494 1567 1864 Fragments arranged by size: 1864 1567 1494 ApaLI G'TGCA_C Cuts at: 3695 3695 Size: 4925 ApoI r'AATT_y Cuts at: 379 587 1929 4278 4534 379 Size: 208 1342 2349 256 770 Fragments arranged by size: 2349 1342 770 256 208 AseI AT'TA_AT Cuts at: 20 20 Size: 4925 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4925 AvaI C'yCGr_G Cuts at: 963 1945 963 Size: 982 3943 BamHI G'GATC_C Cuts at: 1950 1950 Size: 4925 BanI G'GyrC_C Cuts at: 1367 1941 2355 2603 2638 1367 Size: 574 414 248 35 3654 Fragments arranged by size: 3654 574 414 248 35 BanII G_rGCy'C Cuts at: 1210 1939 2969 1210 Size: 729 1030 3166 Fragments arranged by size: 3166 1030 729 BbeI G_GCGC'C Cuts at: 2359 2607 2359 Size: 248 4677 BbsI GAAGACnn'nnnn_ Cuts at: 1908 1908 Size: 4925 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1324 1358 1324 Size: 34 4891 BciVI GTATCCnnnnn_n' Cuts at: 2823 3584 4925 2823 Size: 761 1341 2823 Fragments arranged by size: 2823 1341 761 BclI T'GATC_A Cuts at: 2445 2445 Size: 4925 BglII A'GATC_T Cuts at: 464 2440 4477 464 Size: 1976 2037 912 Fragments arranged by size: 2037 1976 912 BlpI GC'TnA_GC Cuts at: 2037 2037 Size: 4925 Bme1580I G_kGCm'C Cuts at: 820 1337 1408 1492 1576 2550 2643 3699 820 Size: 517 71 84 84 974 93 1056 2046 Fragments arranged by size: 2046 1056 974 517 93 84 84 71 BmrI ACTGGGnnnn_n' Cuts at: 619 775 1404 2137 2218 2553 4697 619 Size: 156 629 733 81 335 2144 847 Fragments arranged by size: 2144 847 733 629 335 156 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 224 3292 224 Size: 3068 1857 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 259 325 758 1180 259 Size: 66 433 422 4004 Fragments arranged by size: 4004 433 422 66 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1261 2101 2980 3472 3770 1261 Size: 840 879 492 298 2416 Fragments arranged by size: 2416 879 840 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 264 4629 264 Size: 4365 560 BsaAI yAC'GTr Cuts at: 751 1653 2278 751 Size: 902 625 3398 Fragments arranged by size: 3398 902 625 BsaBI GATnn'nnATC Cuts at: 6 2439 4476 6 Size: 2433 2037 455 Fragments arranged by size: 2433 2037 455 BsaHI Gr'CG_yC Cuts at: 915 2356 2604 3306 4802 915 Size: 1441 248 702 1496 1038 Fragments arranged by size: 1496 1441 1038 702 248 BsaWI w'CCGG_w Cuts at: 2114 2635 3313 3587 3734 4145 2114 Size: 521 678 274 147 411 2894 Fragments arranged by size: 2894 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 104 134 961 991 1801 1831 104 Size: 30 827 30 810 30 3198 Fragments arranged by size: 3198 827 810 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 346 418 1150 1183 1264 1600 1654 1657 346 Size: 72 732 33 81 336 54 3 3614 Fragments arranged by size: 3614 732 336 81 72 54 33 3 BseYI C'CCAG_C Cuts at: 2350 3108 3685 4181 2350 Size: 758 577 496 3094 Fragments arranged by size: 3094 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1005 1005 Size: 4925 BsiEI CG_ry'CG Cuts at: 104 2467 2513 3721 104 Size: 2363 46 1208 1308 Fragments arranged by size: 2363 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 1210 1939 2717 2907 3699 1210 Size: 729 778 190 792 2436 Fragments arranged by size: 2436 792 778 729 190 BsmI GAATG_Cn' Cuts at: 829 829 Size: 4925 Bsp1286I G_dGCh'C Cuts at: 820 1210 1337 1408 1492 1576 1939 2550 2643 Size: 390 127 71 84 84 363 611 93 Cuts at: 2643 2717 2907 2969 3699 820 Size: 74 190 62 730 2046 Fragments arranged by size: 2046 730 611 390 363 190 127 93 84 84 74 71 62 BspEI T'CCGG_A Cuts at: 4145 4145 Size: 4925 BspHI T'CATG_A Cuts at: 4101 4101 Size: 4925 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1975 2189 2491 2872 1975 Size: 214 302 381 4028 Fragments arranged by size: 4028 381 302 214 BsrBI CCG'CTC Cuts at: 50 3217 3271 4755 4920 50 Size: 3167 54 1484 165 55 Fragments arranged by size: 3167 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 2837 2837 Size: 4925 BsrFI r'CCGG_y Cuts at: 2923 3104 3313 2923 Size: 181 209 4535 Fragments arranged by size: 4535 209 181 BsrGI T'GTAC_A Cuts at: 221 221 Size: 4925 BssSI C'ACGA_G Cuts at: 3196 3554 3196 Size: 358 4567 BstAPI GCAn_nnn'nTGC Cuts at: 2302 2302 Size: 4925 BstBI TT'CG_AA Cuts at: 3286 3286 Size: 4925 BstEII G'GTnAC_C Cuts at: 619 1437 1521 619 Size: 818 84 4023 Fragments arranged by size: 4023 818 84 BstXI CCAn_nnnn'nTGG Cuts at: 632 632 Size: 4925 BstYI r'GATC_y Cuts at: 1 464 1950 2417 2440 2775 3021 4033 4119 Size: 463 1486 467 23 335 246 1012 86 Cuts at: 4119 4131 4477 1 Size: 12 346 449 Fragments arranged by size: 1486 1012 467 463 449 346 335 246 86 23 12 BstZ17I GTA'TAC Cuts at: 1013 1013 Size: 4925 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1757 2875 3056 3219 1757 Size: 1118 181 163 3463 Fragments arranged by size: 3463 1118 181 163 BtsI GCAGTG_nn' Cuts at: 2168 2168 Size: 4925 DraI TTT'AAA Cuts at: 1924 1924 Size: 4925 DraIII CAC_nnn'GTG Cuts at: 405 405 Size: 4925 DrdI GACnn_nn'nnGTC Cuts at: 2631 3489 2631 Size: 858 4067 EaeI y'GGCC_r Cuts at: 450 1680 2510 2684 3075 3102 4447 450 Size: 1230 830 174 391 27 1345 928 Fragments arranged by size: 1345 1230 928 830 391 174 27 EagI C'GGCC_G Cuts at: 2510 2510 Size: 4925 EarI CTCTTCn'nnn_ Cuts at: 2948 3158 2948 Size: 210 4715 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 3133 3443 3589 4735 3133 Size: 310 146 1146 3323 Fragments arranged by size: 3323 1146 310 146 EcoICRI GAG'CTC Cuts at: 1208 1937 1208 Size: 729 4196 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 259 325 588 713 758 990 1180 1242 2750 Size: 66 263 125 45 232 190 62 1508 Cuts at: 2750 3182 3929 4197 259 Size: 432 747 268 987 Fragments arranged by size: 1508 987 747 432 268 263 232 190 125 66 62 45 EcoO109I rG'GnC_Cy Cuts at: 215 553 1058 1226 2064 215 Size: 338 505 168 838 3076 Fragments arranged by size: 3076 838 505 338 168 EcoRI G'AATT_C Cuts at: 379 1929 379 Size: 1550 3375 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1878 2944 1878 Size: 1066 3859 FspI TGC'GCA Cuts at: 2706 2706 Size: 4925 HaeII r_GCGC'y Cuts at: 2359 2607 3629 4608 2359 Size: 248 1022 979 2676 Fragments arranged by size: 2676 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 2767 2797 2799 2829 2767 Size: 30 2 30 4863 Fragments arranged by size: 4863 30 30 2 HincII GTy'rAC Cuts at: 1964 1964 Size: 4925 KasI G'GCGC_C Cuts at: 2355 2603 2355 Size: 248 4677 KpnI G_GTAC'C Cuts at: 1945 1945 Size: 4925 MluI A'CGCG_T Cuts at: 4358 4358 Size: 4925 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 522 1809 3596 3780 522 Size: 1287 1787 184 1667 Fragments arranged by size: 1787 1667 1287 184 MscI TGG'CCA Cuts at: 452 1682 2686 452 Size: 1230 1004 2691 Fragments arranged by size: 2691 1230 1004 MslI CAynn'nnrTG Cuts at: 617 897 2914 3041 617 Size: 280 2017 127 2501 Fragments arranged by size: 2501 2017 280 127 MspA1I CmG'CkG Cuts at: 303 1794 2036 2350 2710 3723 3968 303 Size: 1491 242 314 360 1013 245 1260 Fragments arranged by size: 1491 1260 1013 360 314 245 242 NaeI GCC'GGC Cuts at: 3106 3106 Size: 4925 NarI GG'CG_CC Cuts at: 2356 2604 2356 Size: 248 4677 NgoMIV G'CCGG_C Cuts at: 3104 3104 Size: 4925 NspI r_CATG'y Cuts at: 1978 2478 3385 1978 Size: 500 907 3518 Fragments arranged by size: 3518 907 500 PasI CC'CwG_GG Cuts at: 264 264 Size: 4925 PciI A'CATG_T Cuts at: 3381 3381 Size: 4925 PflMI CCAn_nnn'nTGG Cuts at: 405 405 Size: 4925 PfoI T'CCnGG_A Cuts at: 155 155 Size: 4925 PmeI GTTT'AAAC Cuts at: 1924 1924 Size: 4925 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4064 4096 4064 Size: 32 4893 PpuMI rG'GwC_Cy Cuts at: 215 215 Size: 4925 PshAI GACnn'nnGTC Cuts at: 356 356 Size: 4925 PstI C_TGCA'G Cuts at: 486 1828 1972 2657 486 Size: 1342 144 685 2754 Fragments arranged by size: 2754 1342 685 144 PvuI CG_AT'CG Cuts at: 104 104 Size: 4925 PvuII CAG'CTG Cuts at: 303 2350 2710 303 Size: 2047 360 2518 Fragments arranged by size: 2518 2047 360 RsrII CG'GwC_CG Cuts at: 3120 3120 Size: 4925 SacI G_AGCT'C Cuts at: 1210 1939 1210 Size: 729 4196 SalI G'TCGA_C Cuts at: 1962 1962 Size: 4925 SapI GCTCTTCn'nnn_ Cuts at: 2948 3158 2948 Size: 210 4715 SbfI CC_TGCA'GG Cuts at: 1972 1972 Size: 4925 ScaI AGT'ACT Cuts at: 4442 4442 Size: 4925 SexAI A'CCwGG_T Cuts at: 401 1286 401 Size: 885 4040 SfcI C'TryA_G Cuts at: 33 246 482 759 1824 1968 2653 3646 3837 Size: 213 236 277 1065 144 685 993 191 Cuts at: 3837 33 Size: 1121 Fragments arranged by size: 1121 1065 993 685 277 236 213 191 144 SfoI GGC'GCC Cuts at: 2357 2605 2357 Size: 248 4677 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4925 SmaI CCC'GGG Cuts at: 1947 1947 Size: 4925 SmlI C'TyrA_G Cuts at: 1240 2080 2995 3487 3749 1240 Size: 840 915 492 262 2416 Fragments arranged by size: 2416 915 840 492 262 SnaBI TAC'GTA Cuts at: 1653 1653 Size: 4925 SpeI A'CTAG_T Cuts at: 67 67 Size: 4925 SphI G_CATG'C Cuts at: 1978 2478 1978 Size: 500 4425 StuI AGG'CCT Cuts at: 934 934 Size: 4925 StyI C'CwwG_G Cuts at: 322 2059 2413 322 Size: 1737 354 2834 Fragments arranged by size: 2834 1737 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 193 2508 2641 3100 3309 193 Size: 2315 133 459 209 1809 Fragments arranged by size: 2315 1809 459 209 133 TatI w'GTAC_w Cuts at: 221 4440 221 Size: 4219 706 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 104 136 452 484 4442 4474 104 Size: 32 316 32 3958 32 555 Fragments arranged by size: 3958 555 316 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1884 2722 1884 Size: 838 4087 XbaI T'CTAG_A Cuts at: 1956 1956 Size: 4925 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2420 2420 Size: 4925 XmaI C'CCGG_G Cuts at: 1945 1945 Size: 4925 XmnI GAAnn'nnTTC Cuts at: 344 1144 344 Size: 800 4125 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI Bpu10I BsiWI BsmBI BssHII Bsu36I BtgI ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI NruI NsiI PacI PmlI PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SrfI SspI SwaI XhoI ZraI