Restriction map for pF1KA1555
##################################
# NO CUT ENZYMES (   55 enzymes) #
##################################

AccI        AccIII      AgeI        AhdI        Aor51HI     
AscI        AseI        AsnI        AspEI       AspI        
AviII       AvrII       BciVI       BclI        BlnI        
BseAI       BsiWI       BsmBI       BspEI       BspHI       
BssHII      Bst1107I    BstZ17I     DrdI        Eam1105I    
Eco47III    EcoRV       FbaI        FseI        FspI        
HpaI        MfeI        MluI        MroI        MunI        
NheI        NotI        NruI        PacI        PflFI       
PinAI       PmeI        PshBI       PvuI        RcaI        
SalI        SbfI        SexAI       SgrAI       SnaBI       
Sse8387I    SspI        SwaI        Tth111I     XbaI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AatII        5332  GACGTC               GACGT^C
Acc65I       6169  GGTACC               G^GTACC
AclI         2992  AACGTT               AA^CGTT
AflII        1101  CTTAAG               C^TTAAG
Alw44I       7004  GTGCAC               G^TGCAC
ApaLI        7004  GTGCAC               G^TGCAC
Asp700I      6519  GAACATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      6169  GGTACC               G^GTACC
BalI         4991  TGGCCA               TGG^CCA
BbeI         5640  GGCGCC               GGCGC^C
BfrI         1101  CTTAAG               C^TTAAG
BsaWI        2344  TCCGGT               [AT]^CCGG[AT]
BsmI          307  GCATTC               GAATGC(1/-1)
BspDI        1123  ATCGAT               AT^CGAT
BstBI        5197  TTCGAA               TT^CGAA
ClaI         1123  ATCGAT               AT^CGAT
CpoI         1282  CGGACCG              CG^G[AT]CCG
DraIII       3281  CACATGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoT22I      2095  ATGCAT               ATGCA^T
HindIII      4586  AAGCTT               A^AGCTT
KasI         5640  GGCGCC               G^GCGCC
KpnI         6169  GGTACC               GGTAC^C
KspI         5244  CCGCGG               CCGC^GG
MluNI        4991  TGGCCA               TGG^CCA
MscI         4991  TGGCCA               TGG^CCA
NarI         5640  GGCGCC               GG^CGCC
NdeI         1938  CATATG               CA^TATG
NsiI         2095  ATGCAT               ATGCA^T
NspV         5197  TTCGAA               TT^CGAA
PshAI        5900  GACCGTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     2992  AACGTT               AA^CGTT
RsrII        1282  CGGACCG              CG^G[AT]CCG
SacII        5244  CCGCGG               CCGC^GG
SfiI         6699  GGCCCGGGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         5640  GGCGCC               GGC^GCC
SfuI         5197  TTCGAA               TT^CGAA
SpeI          862  ACTAGT               A^CTAGT
XmnI         6519  GAACATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

BbrPI        1732  CACGTG               CAC^GTG
             6040  CACGTG              
BglII        4019  AGATCT               A^GATCT
             5100  AGATCT              
BsaAI        1732  CACGTG               [CT]AC^GT[GA]
             6040  CACGTG              
BsaBI        5187  GATCAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             6579  GATTCCCATC          
Bsp1407I     4001  TGTACA               T^GTACA
             4901  TGTACA              
BspLU11I     2921  ACATGT               A^CATGT
             6147  ACATGT              
BsrBI        2832  CCGCTC               CCGCTC(-3/-3)
              796  GAGCGG              
BsrDI        2807  GCAATG               GCAATG(2/0)
             3997  GCAATG              
BsrGI        4001  TGTACA               T^GTACA
             4901  TGTACA              
BssSI        5738  CACGAG               CACGAG(-5/-1)
             3451  CTCGTG              
BstAPI       3447  GCATCTCGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             5420  GCAAAAAGTGC         
BstEII        877  GGTCACC              G^GT[ACGT]ACC
             4026  GGTCACC             
BstPI         877  GGTCACC              G^GT[ACGT]ACC
             4026  GGTCACC             
DraI         4824  TTTAAA               TTT^AAA
             5371  TTTAAA              
EcoO65I       877  GGTCACC              G^GT[ACGT]ACC
             4026  GGTCACC             
EcoRI        3481  GAATTC               G^AATTC
             5279  GAATTC              
MamI         5187  GATCAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             6579  GATTCCCATC          
PmaCI        1732  CACGTG               CAC^GTG
             6040  CACGTG              
PmlI         1732  CACGTG               CAC^GTG
             6040  CACGTG              
ScaI          584  AGTACT               AGT^ACT
             1162  AGTACT              
SspBI        4001  TGTACA               T^GTACA
             4901  TGTACA              


#########################################
# 3 OR MORE CUT ENZYMES (  144 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AflIII      AluI        AlwI        AlwNI       AocI        
ApaI        ApoI        AspHI       AvaI        AvaII       
BamHI       BanI        BanII       BbsI        BbvCI       
BbvI        BcgI        BcnI        BfaI        BglI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaHI       BsaI        BsaJI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrFI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstXI       BstYI       Bsu36I      Cac8I       CelII       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EagI        EarI        
EclXI       Eco52I      Eco81I      EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NaeI        NciI        
NcoI        NdeII       NgoMIV      NlaIII      NlaIV       
NspI        PaeR7I      PflMI       PleI        PpuMI       
PstI        PvuII       RsaI        SacI        SapI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmaI        SmlI        SphI        StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XcmI        
XhoI        XhoII       XmaCI       XmaI        
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