Restriction map for pF1KA1503
##################################
# NO CUT ENZYMES (   90 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AflIII      
AgeI        AocI        Aor51HI     AscI        AseI        
AsnI        Asp700I     Asp718I     AviII       AvrII       
BamHI       BbrPI       BcgI        BfrI        BlnI        
BsaBI       BsiEI       BsiWI       BsmBI       BspDI       
BspLU11I    BsrBI       BsrFI       BssSI       Bst1107I    
BstEII      BstPI       BstZ17I     Bsu36I      Cfr10I      
ClaI        CpoI        DraI        EagI        EclXI       
Eco47III    Eco52I      Eco81I      EcoO65I     EcoRI       
EcoRV       FseI        FspI        HindIII     HpaI        
KpnI        KspI        MamI        MfeI        MluI        
MunI        NaeI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        PacI        PaeR7I      
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SgrAI       SnaBI       SpeI        Sse8387I    SspI        
StuI        SwaI        XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AccI          415  GTCTAC               GT^[AC][GT]AC
AccIII       1633  TCCGGA               T^CCGGA
AhdI         1806  GACATCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI          492  GGGCCC               GGGCC^C
AspEI        1806  GACATCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1675  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbeI          945  GGCGCC               GGCGC^C
BbsI         1363  GAAGAC               GAAGAC(2/6)
BbvCI        1427  CCTCAGC              CCTCAGC(-5/-2)
BglII         545  AGATCT               A^GATCT
BlpI          142  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      142  GCTGAGC              GC^T[ACGT]AGC
BpuAI        1363  GAAGAC               GAAGAC(2/6)
BsaAI        1618  TACGTG               [CT]AC^GT[GA]
BsaWI        1633  TCCGGA               [AT]^CCGG[AT]
BseAI        1633  TCCGGA               T^CCGGA
Bsp1407I      393  TGTACA               T^GTACA
BspEI        1633  TCCGGA               T^CCGGA
BspMI        1687  ACCTGC               ACCTGC(4/8)
BsrDI        1850  CATTGC               GCAATG(2/0)
BsrGI         393  TGTACA               T^GTACA
BssHII       1642  GCGCGC               G^CGCGC
BstBI        1784  TTCGAA               TT^CGAA
BstXI        2176  CCAGCACTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         142  GCTGAGC              GC^T[ACGT]AGC
DrdI         1008  GACCTTTTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          169  CCACGG               C^C[GA][CT]GG
Eam1105I     1806  GACATCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI        1481  CCTTCCACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I       852  ATGCAT               ATGCA^T
HaeII         945  GGCGCC               [GA]GCGC^[CT]
HgaI         1665  GCGTC                GACGC(5/10)
KasI          945  GGCGCC               G^GCGCC
MaeIII       1175  GTGAC                ^GT[ACGT]AC
MroI         1633  TCCGGA               T^CCGGA
MslI          681  CATGAACATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI          945  GGCGCC               GG^CGCC
NsiI          852  ATGCAT               ATGCA^T
NspV         1784  TTCGAA               TT^CGAA
PflFI        1675  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PvuII         880  CAGCTG               CAG^CTG
SacI          145  GAGCTC               GAGCT^C
SapI         1158  GCTCTTC              GCTCTTC(1/4)
SfoI          945  GGCGCC               GGC^GCC
SfuI         1784  TTCGAA               TT^CGAA
SmaI         2350  CCCGGG               CCC^GGG
SspBI         393  TGTACA               T^GTACA
Tsp45I       1175  GTGAC                ^GT[GC]AC
Tth111I      1675  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         1769  CCATTGATGAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI        2350  CCCGGG               C^CCGGG
XmaI         2350  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AcsI          810  AAATTT               [GA]^AATT[CT]
             1960  GAATTT              
AcyI          945  GGCGCC               G[GA]^CG[CT]C
             1664  GGCGTC              
Alw44I       1651  GTGCAC               G^TGCAC
             1741  GTGCAC              
AlwNI        1414  CAGAACCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2255  CAGTCTCTG           
ApaLI        1651  GTGCAC               G^TGCAC
             1741  GTGCAC              
ApoI          810  AAATTT               [GA]^AATT[CT]
             1960  GAATTT              
BalI           89  TGGCCA               TGG^CCA
             1600  TGGCCA              
BanI          945  GGCGCC               G^G[CT][GA]CC
             1240  GGTGCC              
BciVI        1842  GGATAC               GTATCC(6/5)
             1331  GTATCC              
BclI         1091  TGATCA               T^GATCA
             1421  TGATCA              
BglI           73  GCCACTCGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2349  GCCCGGGAGGC         
BsaHI         945  GGCGCC               G[GA]^CG[CT]C
             1664  GGCGTC              
BsaI          736  GAGACC               GGTCTC(1/5)
             2307  GGTCTC              
BsmI          108  GAATGC               GAATGC(1/-1)
             1829  GCATTC              
BspHI        1052  TCATGA               T^CATGA
             1682  TCATGA              
DraIII        191  CACAGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1459  CACGGCGTG           
EcoT14I       152  CCAAGG               C^C[AT][AT]GG
             1061  CCAAGG              
FbaI         1091  TGATCA               T^GATCA
             1421  TGATCA              
Hin1I         945  GGCGCC               G[GA]^CG[CT]C
             1664  GGCGTC              
HincII       1117  GTCAAC               GT[CT]^[GA]AC
             1891  GTCAAC              
HindII       1117  GTCAAC               GT[CT]^[GA]AC
             1891  GTCAAC              
MluNI          89  TGGCCA               TGG^CCA
             1600  TGGCCA              
MscI           89  TGGCCA               TGG^CCA
             1600  TGGCCA              
MseI         2099  TTAA                 T^TAA
             2117  TTAA                
MspA1I        880  CAGCTG               C[AC]G^C[GT]G
             1197  CCGCTG              
PflMI         443  CCATCACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1038  CCAGGAGTTGG         
RcaI         1052  TCATGA               T^CATGA
             1682  TCATGA              
SmlI          185  CTCAAG               C^T[CT][GA]AG
             2209  CTTGAG              
SphI          386  GCATGC               GCATG^C
             1880  GCATGC              
StyI          152  CCAAGG               C^C[AT][AT]GG
             1061  CCAAGG              
Tru9I        2099  TTAA                 T^TAA
             2117  TTAA                
Van91I        443  CCATCACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1038  CCAGGAGTTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   84 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AspHI       AvaI        AvaII       BanII       BbvI        
BcnI        BfaI        BmyI        BpmI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstUI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       EaeI        EarI        EcoO109I    
EcoRII      FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MboI        MboII       MflI        MnlI        MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PleI        PpuMI       
PstI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        TaqI        TfiI        TseI        
Tsp509I     TspRI       TthHB8I     XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com