Restriction map for pF1KA1503
 (Linear) (Six-base) MAPSORT of: KIAA1503.seq  Check: 6584  from: 1  to: 2370



KIAA1503.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:36  ..

AccI GT'mk_AC

Cuts at:      0     416    2370 
   Size:        416    1954
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     391    1045    2370 
   Size:        391     654    1325

  Fragments arranged by size: 

                1325     654     391
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1811    2370 
   Size:       1811     559
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     125    2370 
   Size:        125    2245
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     820     852    2370 
   Size:        820      32    1518

  Fragments arranged by size: 

                1518     820      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1419    2260    2370 
   Size:       1419     841     110

  Fragments arranged by size: 

                1419     841     110
 
ApaI G_GGCC'C

Cuts at:      0     496    2370 
   Size:        496    1874
 
ApaLI G'TGCA_C

Cuts at:      0    1651    1741    2370 
   Size:       1651      90     629

  Fragments arranged by size: 

                1651     629      90
 
ApoI r'AATT_y

Cuts at:      0     810    1960    2370 
   Size:        810    1150     410

  Fragments arranged by size: 

                1150     810     410
 
AvaI C'yCGr_G

Cuts at:      0      77     214     245    1139    2350    2370 
   Size:         77     137      31     894    1211      20

  Fragments arranged by size: 

                1211     894     137      77      31      20
 
BanI G'GyrC_C

Cuts at:      0     945    1240    2370 
   Size:        945     295    1130

  Fragments arranged by size: 

                1130     945     295
 
BanII G_rGCy'C

Cuts at:      0     149     215     496    1616    2216    2351    2370 
   Size:        149      66     281    1120     600     135      19

  Fragments arranged by size: 

                1120     600     281     149     135      66      19
 
BbeI G_GCGC'C

Cuts at:      0     949    2370 
   Size:        949    1421
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1370    2370 
   Size:       1370    1000
 
BbvCI CC'TCA_GC

Cuts at:      0    1428    2370 
   Size:       1428     942
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1342    1836    2370 
   Size:       1342     494     534

  Fragments arranged by size: 

                1342     534     494
 
BclI T'GATC_A

Cuts at:      0    1091    1421    2370 
   Size:       1091     330     949

  Fragments arranged by size: 

                1091     949     330
 
BfrBI ATG'CAT

Cuts at:      0     854    2370 
   Size:        854    1516
 
BglI GCCn_nnn'nGGC

Cuts at:      0      79    2355    2370 
   Size:         79    2276      15

  Fragments arranged by size: 

                2276      79      15
 
BglII A'GATC_T

Cuts at:      0     545    2370 
   Size:        545    1825
 
BlpI GC'TnA_GC

Cuts at:      0     143    2370 
   Size:        143    2227
 
Bme1580I G_kGCm'C

Cuts at:      0     496     653     786    1245    1655    1745    2370 
   Size:        496     157     133     459     410      90     625

  Fragments arranged by size: 

                 625     496     459     410     157     133      90
 
BmgBI CAC'GTC

Cuts at:      0    1452    2370 
   Size:       1452     918
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1620    2248    2370 
   Size:       1620     628     122

  Fragments arranged by size: 

                1620     628     122
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     664     844    1294    1420    1630    2370 
   Size:        664     180     450     126     210     740

  Fragments arranged by size: 

                 740     664     450     210     180     126
 
Bpu10I CC'TnA_GC

Cuts at:      0     164    1085    1428    2345    2370 
   Size:        164     921     343     917      25

  Fragments arranged by size: 

                 921     917     343     164      25
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     170    2230    2370 
   Size:        170    2060     140

  Fragments arranged by size: 

                2060     170     140
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     730    2313    2370 
   Size:        730    1583      57

  Fragments arranged by size: 

                1583     730      57
 
BsaAI yAC'GTr

Cuts at:      0    1620    2370 
   Size:       1620     750
 
BsaHI Gr'CG_yC

Cuts at:      0     946    1665    2370 
   Size:        946     719     705

  Fragments arranged by size: 

                 946     719     705
 
BsaWI w'CCGG_w

Cuts at:      0    1633    2370 
   Size:       1633     737
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     820     850    2370 
   Size:        820      30    1520

  Fragments arranged by size: 

                1520     820      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     172     841    1973    2370 
   Size:        172     669    1132     397

  Fragments arranged by size: 

                1132     669     397     172
 
BseYI C'CCAG_C

Cuts at:      0     381    1199    1646    2175    2191    2282    2370 
   Size:        381     818     447     529      16      91      88

  Fragments arranged by size: 

                 818     529     447     381      91      88      16
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     493     586     904    1288    2370 
   Size:        493      93     318     384    1082

  Fragments arranged by size: 

                1082     493     384     318      93
 
BsiHKAI G_wGCw'C

Cuts at:      0     149    1655    1704    1745    2370 
   Size:        149    1506      49      41     625

  Fragments arranged by size: 

                1506     625     149      49      41
 
BsmI GAATG_Cn'

Cuts at:      0     114    1829    2370 
   Size:        114    1715     541

  Fragments arranged by size: 

                1715     541     114
 
Bsp1286I G_dGCh'C

Cuts at:      0     149     215     496     653     786    1245    1616    1655 
   Size:        149      66     281     157     133     459     371      39

Cuts at:   1655    1704    1745    2216    2351    2370 
   Size:         49      41     471     135      19

  Fragments arranged by size: 

                 471     459     371     281     157     149     135     133
                  66      49      41      39      19
 
BspEI T'CCGG_A

Cuts at:      0    1633    2370 
   Size:       1633     737
 
BspHI T'CATG_A

Cuts at:      0    1052    1682    2370 
   Size:       1052     630     688

  Fragments arranged by size: 

                1052     688     630
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1696    2370 
   Size:       1696     674
 
BsrDI GCAATG_nn'

Cuts at:      0    1849    2370 
   Size:       1849     521
 
BsrGI T'GTAC_A

Cuts at:      0     393    2370 
   Size:        393    1977
 
BssHII G'CGCG_C

Cuts at:      0    1642    2370 
   Size:       1642     728
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     851    1311    1464    2370 
   Size:        851     460     153     906

  Fragments arranged by size: 

                 906     851     460     153
 
BstBI TT'CG_AA

Cuts at:      0    1785    2370 
   Size:       1785     585
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2183    2370 
   Size:       2183     187
 
BstYI r'GATC_y

Cuts at:      0     545     971    1539    2370 
   Size:        545     426     568     831

  Fragments arranged by size: 

                 831     568     545     426
 
BtgI C'CryG_G

Cuts at:      0     169    2370 
   Size:        169    2201
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2189    2370 
   Size:       2189     181
 
BtsI GCAGTG_nn'

Cuts at:      0     281    2370 
   Size:        281    2089
 
DraIII CAC_nnn'GTG

Cuts at:      0     196    1464    2370 
   Size:        196    1268     906

  Fragments arranged by size: 

                1268     906     196
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1014    2370 
   Size:       1014    1356
 
EaeI y'GGCC_r

Cuts at:      0      36      89    1600    2145    2370 
   Size:         36      53    1511     545     225

  Fragments arranged by size: 

                1511     545     225      53      36
 
EarI CTCTTCn'nnn_

Cuts at:      0     241    1165    1993    2370 
   Size:        241     924     828     377

  Fragments arranged by size: 

                 924     828     377     241
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     283    2370 
   Size:        283    2087
 
EcoICRI GAG'CTC

Cuts at:      0     147    2370 
   Size:        147    2223
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     391     664     844    1045    1294    1420    1630    2370 
   Size:        391     273     180     201     249     126     210     740

  Fragments arranged by size: 

                 740     391     273     249     210     201     180     126
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1485    2370 
   Size:       1485     885
 
EcoO109I rG'GnC_Cy

Cuts at:      0     181     321     492     493     817    1219    1395    2370 
   Size:        181     140     171       1     324     402     176     975

  Fragments arranged by size: 

                 975     402     324     181     176     171     140       1
            
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     252    2370 
   Size:        252    2118
 
HaeII r_GCGC'y

Cuts at:      0     949    2370 
   Size:        949    1421
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     615     647    1792    1797    1824    1829    2370 
   Size:        615      32    1145       5      27       5     541

  Fragments arranged by size: 

                1145     615     541      32      27       5       5
 
HincII GTy'rAC

Cuts at:      0    1119    1893    2370 
   Size:       1119     774     477

  Fragments arranged by size: 

                1119     774     477
 
KasI G'GCGC_C

Cuts at:      0     945    2370 
   Size:        945    1425
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     155    1820    2370 
   Size:        155    1665     550

  Fragments arranged by size: 

                1665     550     155
 
MscI TGG'CCA

Cuts at:      0      91    1602    2370 
   Size:         91    1511     768

  Fragments arranged by size: 

                1511     768      91
 
MslI CAynn'nnrTG

Cuts at:      0     685    2370 
   Size:        685    1685
 
MspA1I CmG'CkG

Cuts at:      0     882    1199    2370 
   Size:        882     317    1171

  Fragments arranged by size: 

                1171     882     317
 
NarI GG'CG_CC

Cuts at:      0     946    2370 
   Size:        946    1424
 
NsiI A_TGCA'T

Cuts at:      0     856    2370 
   Size:        856    1514
 
NspI r_CATG'y

Cuts at:      0     390    1551    1884    2370 
   Size:        390    1161     333     486

  Fragments arranged by size: 

                1161     486     390     333
 
PasI CC'CwG_GG

Cuts at:      0    1245    2370 
   Size:       1245    1125
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     449    1044    2370 
   Size:        449     595    1326

  Fragments arranged by size: 

                1326     595     449
 
PfoI T'CCnGG_A

Cuts at:      0     974    1334    2370 
   Size:        974     360    1036

  Fragments arranged by size: 

                1036     974     360
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     820     852    2370 
   Size:        820      32    1518

  Fragments arranged by size: 

                1518     820      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     181     321     817    1219    1395    2370 
   Size:        181     140     496     402     176     975

  Fragments arranged by size: 

                 975     496     402     181     176     140
 
PspOMI G'GGCC_C

Cuts at:      0     492    2370 
   Size:        492    1878
 
PstI C_TGCA'G

Cuts at:      0     140     675    1949    2369    2370 
   Size:        140     535    1274     420       1

  Fragments arranged by size: 

                1274     535     420     140       1
 
PvuII CAG'CTG

Cuts at:      0     882    2370 
   Size:        882    1488
 
SacI G_AGCT'C

Cuts at:      0     149    2370 
   Size:        149    2221
 
SanDI GG'GwC_CC

Cuts at:      0    1219    2370 
   Size:       1219    1151
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1165    2370 
   Size:       1165    1205
 
SfcI C'TryA_G

Cuts at:      0     136     326     671    1945    2365    2370 
   Size:        136     190     345    1274     420       5

  Fragments arranged by size: 

                1274     420     345     190     136       5
 
SfoI GGC'GCC

Cuts at:      0     947    2370 
   Size:        947    1423
 
SmaI CCC'GGG

Cuts at:      0    2352    2370 
   Size:       2352      18
 
SmlI C'TyrA_G

Cuts at:      0     185    2209    2370 
   Size:        185    2024     161

  Fragments arranged by size: 

                2024     185     161
 
SphI G_CATG'C

Cuts at:      0     390    1884    2370 
   Size:        390    1494     486

  Fragments arranged by size: 

                1494     486     390
 
StyI C'CwwG_G

Cuts at:      0     152    1061    2370 
   Size:        152     909    1309

  Fragments arranged by size: 

                1309     909     152
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1638    2370 
   Size:       1638     732
 
TatI w'GTAC_w

Cuts at:      0     393    1904    2370 
   Size:        393    1511     466

  Fragments arranged by size: 

                1511     466     393
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     262     294     895     927     956     988    2156    2188 
   Size:        262      32     601      32      29      32    1168      32

Cuts at:   2188    2370 
   Size:        182

  Fragments arranged by size: 

                1168     601     262     182      32      32      32      32
                  29
 
Tth111I GACn'n_nGTC

Cuts at:      0    1678    2370 
   Size:       1678     692
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1776    2370 
   Size:       1776     594
 
XmaI C'CCGG_G

Cuts at:      0    2350    2370 
   Size:       2350      20
 

 Enzymes that do cut:

     AccI     AcuI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AvaI     BanI    BanII     BbeI     BbsI    BbvCI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrDI    BsrGI   BssHII   BstAPI    BstBI    BstXI    BstYI
     BtgI    BtgZI     BtsI   DraIII     DrdI     EaeI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI    HaeII    Hin4I   HincII
     KasI     MmeI     MscI     MslI   MspA1I     NarI     NsiI     NspI
     PasI    PflMI     PfoI     PpiI    PpuMI   PspOMI     PstI    PvuII
     SacI    SanDI     SapI     SfcI     SfoI     SmaI     SmlI     SphI
     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AleI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BcgI
     BmtI     BplI    BsaBI    BsiEI    BsiWI    BsmBI    BsrBI    BsrFI
    BssSI   BstEII  BstZ17I   Bsu36I     ClaI    CspCI     DraI     EagI
    EcoRI    EcoRV     FseI     FspI    FspAI  HindIII     HpaI     KpnI
     MfeI     MluI     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     PacI     PciI     PmeI     PmlI    PshAI     PsiI    PspXI
     PsrI     PvuI    RsrII    SacII     SalI     SbfI     ScaI    SexAI
     SfiI     SgfI    SgrAI    SnaBI     SpeI     SrfI     SspI     StuI
     SwaI     XbaI     XhoI     XmnI     ZraI
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