################################## # NO CUT ENZYMES ( 102 enzymes) # ################################## AatII Acc65I AccI AccIII AcyI AflIII AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BamHI BbeI BbrPI BcgI BciVI BglI BlnI BsaAI BsaHI BsaWI BseAI BsiWI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrFI BsrGI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I Cfr10I ClaI CpoI DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT22I FseI FspI Hin1I KasI KpnI KspI MluI MroI NaeI NarI NgoMIV NotI NruI NsiI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SwaI Tth111I Van91I XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AclI 1421 AACGTT AA^CGTT AhdI 555 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 988 GATCC GGATC(4/5) AspEI 555 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BanI 472 GGTGCC G^G[CT][GA]CC BbsI 2094 GAAGAC GAAGAC(2/6) BbvCI 2569 CCTCAGC CCTCAGC(-5/-2) BpuAI 2094 GAAGAC GAAGAC(2/6) BsaBI 982 GATCTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1890 GGTCTC GGTCTC(1/5) BsmI 1590 GAATGC GAATGC(1/-1) BspHI 957 TCATGA T^CATGA BspMI 2172 ACCTGC ACCTGC(4/8) BsrDI 2136 CATTGC GCAATG(2/0) BssHII 188 GCGCGC G^CGCGC BssSI 181 CACGAG CACGAG(-5/-1) DraI 600 TTTAAA TTT^AAA Eam1105I 555 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 100 GAAGAG CTCTTC(1/4) EcoRV 2423 GATATC GAT^ATC FauI 95 GCGGG CCCGC(4/6) HaeII 2226 AGCGCC [GA]GCGC^[CT] HgaI 198 GACGC GACGC(5/10) HindIII 342 AAGCTT A^AGCTT HpaI 1132 GTTAAC GTT^AAC Ksp632I 100 GAAGAG CTCTTC(1/4) MaeII 1422 ACGT A^CGT MamI 982 GATCTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NcoI 2453 CCATGG C^CATGG NdeI 1976 CATATG CA^TATG PaeR7I 193 CTCGAG C^TCGAG Psp1406I 1421 AACGTT AA^CGTT PvuII 22 CAGCTG CAG^CTG RcaI 957 TCATGA T^CATGA SapI 100 GAAGAGC GCTCTTC(1/4) ScaI 113 AGTACT AGT^ACT SmaI 2538 CCCGGG CCC^GGG SphI 834 GCATGC GCATG^C SspI 1882 AATATT AAT^ATT StuI 2269 AGGCCT AGG^CCT TfiI 1849 GAATC G^A[AT]TC Tsp45I 1550 GTGAC ^GT[GC]AC XbaI 984 TCTAGA T^CTAGA XcmI 459 CCAGTTTGCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 193 CTCGAG C^TCGAG XmaCI 2538 CCCGGG C^CCGGG XmaI 2538 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AflII 510 CTTAAG C^TTAAG 1941 CTTAAG AvaI 193 CTCGAG C^[CT]CG[GA]G 2538 CCCGGG BalI 50 TGGCCA TGG^CCA 2478 TGGCCA BanII 133 GAGCTC G[GA]GC[CT]^C 2300 GAGCTC BclI 216 TGATCA T^GATCA 1299 TGATCA BfrI 510 CTTAAG C^TTAAG 1941 CTTAAG BlpI 135 GCTCAGC GC^T[ACGT]AGC 2372 GCTCAGC Bpu1102I 135 GCTCAGC GC^T[ACGT]AGC 2372 GCTCAGC BsiEI 162 CGGTCG CG[GA][CT]^CG 524 CGACCG BsmBI 196 GAGACG CGTCTC(1/5) 493 GAGACG BsoBI 193 CTCGAG C^[CT]CG[GA]G 2538 CCCGGG CelII 135 GCTCAGC GC^T[ACGT]AGC 2372 GCTCAGC DraII 553 GGGACCC [GA]G^G[ACGT]CC[CT] 2541 GGGACCC DsaI 527 CCGTGG C^C[GA][CT]GG 2453 CCATGG EaeI 50 TGGCCA [CT]^GGCC[GA] 2478 TGGCCA EcoO109I 553 GGGACCC [GA]G^G[ACGT]CC[CT] 2541 GGGACCC EcoT14I 713 CCAAGG C^C[AT][AT]GG 2453 CCATGG FbaI 216 TGATCA T^GATCA 1299 TGATCA HapII 1024 CCGG C^CGG 2539 CCGG HincII 1132 GTTAAC GT[CT]^[GA]AC 2037 GTTGAC HindII 1132 GTTAAC GT[CT]^[GA]AC 2037 GTTGAC HpaII 1024 CCGG C^CGG 2539 CCGG MluNI 50 TGGCCA TGG^CCA 2478 TGGCCA MscI 50 TGGCCA TGG^CCA 2478 TGGCCA MspI 1024 CCGG C^CGG 2539 CCGG NheI 391 GCTAGC G^CTAGC 1185 GCTAGC NspI 834 GCATGC [GA]CATG^[CT] 2202 ACATGC PpuMI 553 GGGACCC [GA]G^G[AT]CC[CT] 2541 GGGACCC PstI 659 CTGCAG CTGCA^G 926 CTGCAG SacI 133 GAGCTC GAGCT^C 2300 GAGCTC SfcI 659 CTGCAG C^T[GA][CT]AG 926 CTGCAG StyI 713 CCAAGG C^C[AT][AT]GG 2453 CCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 77 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwNI ApoI AspHI AvaII BbvI BcnI BfaI BglII BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HhaI HinP1I HinfI HphI ItaI MaeI MaeIII MboI MboII MfeI MflI MnlI MseI MslI MspA1I MunI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SmlI TaqI Tru9I TseI Tsp509I TspRI TthHB8I XhoII