(Circular) (Six-base) MAPSORT of: pF1KA1375.seq Check: 3160 from: 1 to: 5717 pF1KA1375 5717 bp With 182 enzymes: SgfI * June 20, 2008 10:21 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2289 2289 Size: 5717 AccI GT'mk_AC Cuts at: 2755 2755 Size: 5717 Acc65I G'GTAC_C Cuts at: 2733 2733 Size: 5717 AclI AA'CG_TT Cuts at: 1530 5557 1530 Size: 4027 1690 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1575 3542 3974 4327 4989 1575 Size: 1967 432 353 662 2303 Fragments arranged by size: 2303 1967 662 432 353 AflII C'TTAA_G Cuts at: 618 2049 618 Size: 1431 4286 AflIII A'CryG_T Cuts at: 3069 4869 5150 3069 Size: 1800 281 3636 Fragments arranged by size: 3636 1800 281 AhdI GACnn_n'nnGTC Cuts at: 668 668 Size: 5717 AleI CACnn'nnGTG Cuts at: 1117 1666 1117 Size: 549 5168 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 887 887 Size: 5717 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 335 367 335 Size: 32 5685 AlwNI CAG_nnn'CTG Cuts at: 525 655 1690 3022 4460 525 Size: 130 1035 1332 1438 1782 Fragments arranged by size: 1782 1438 1332 1035 130 ApaLI G'TGCA_C Cuts at: 4555 4555 Size: 5717 ApoI r'AATT_y Cuts at: 349 913 1156 1519 1836 2011 2070 2721 5070 Size: 564 243 363 317 175 59 651 2349 Cuts at: 5070 5326 349 Size: 256 740 Fragments arranged by size: 2349 740 651 564 363 317 256 243 175 59 AseI AT'TA_AT Cuts at: 20 20 Size: 5717 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5717 AvaI C'yCGr_G Cuts at: 301 2646 2737 301 Size: 2345 91 3281 Fragments arranged by size: 3281 2345 91 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2269 2302 2599 2632 2269 Size: 33 297 33 5354 Fragments arranged by size: 5354 297 33 33 BamHI G'GATC_C Cuts at: 2742 2742 Size: 5717 BanI G'GyrC_C Cuts at: 580 2733 3147 3395 3430 580 Size: 2153 414 248 35 2867 Fragments arranged by size: 2867 2153 414 248 35 BanII G_rGCy'C Cuts at: 245 2412 2731 3761 245 Size: 2167 319 1030 2201 Fragments arranged by size: 2201 2167 1030 319 BbeI G_GCGC'C Cuts at: 3151 3399 3151 Size: 248 5469 BbsI GAAGACnn'nnnn_ Cuts at: 2209 2209 Size: 5717 BbvCI CC'TCA_GC Cuts at: 2678 2678 Size: 5717 BciVI GTATCCnnnnn_n' Cuts at: 3615 4671 5717 3615 Size: 1056 1046 3615 Fragments arranged by size: 3615 1056 1046 BclI T'GATC_A Cuts at: 324 1407 3237 324 Size: 1083 1830 2804 Fragments arranged by size: 2804 1830 1083 BglII A'GATC_T Cuts at: 371 1089 1372 3232 5269 371 Size: 718 283 1860 2037 819 Fragments arranged by size: 2037 1860 819 718 283 BlpI GC'TnA_GC Cuts at: 244 2481 2829 244 Size: 2237 348 3132 Fragments arranged by size: 3132 2237 348 Bme1580I G_kGCm'C Cuts at: 1029 3342 3435 4559 1029 Size: 2313 93 1124 2187 Fragments arranged by size: 2313 2187 1124 93 BmrI ACTGGGnnnn_n' Cuts at: 213 2929 3010 3345 5489 213 Size: 2716 81 335 2144 441 Fragments arranged by size: 2716 2144 441 335 81 BmtI G_CTAG'C Cuts at: 503 1297 503 Size: 794 4923 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2236 4084 2236 Size: 1848 3869 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 632 1355 1834 2315 2417 632 Size: 723 479 481 102 3932 Fragments arranged by size: 3932 723 481 479 102 Bpu10I CC'TnA_GC Cuts at: 453 495 1691 2678 453 Size: 42 1196 987 3492 Fragments arranged by size: 3492 1196 987 42 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 677 1859 2893 3772 4486 4784 677 Size: 1182 1034 879 714 298 1610 Fragments arranged by size: 1610 1182 1034 879 714 298 BsaI GGTCTCn'nnnn_ Cuts at: 2004 5421 2004 Size: 3417 2300 BsaAI yAC'GTr Cuts at: 3070 3070 Size: 5717 BsaBI GATnn'nnATC Cuts at: 6 1094 3231 5268 6 Size: 1088 2137 2037 455 Fragments arranged by size: 2137 2037 1088 455 BsaHI Gr'CG_yC Cuts at: 3148 3396 4098 5594 3148 Size: 248 702 1496 3271 Fragments arranged by size: 3271 1496 702 248 BsaWI w'CCGG_w Cuts at: 2906 3427 4105 4516 4663 4937 2906 Size: 521 678 411 147 274 3686 Fragments arranged by size: 3686 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 416 446 1328 1358 416 Size: 30 882 30 4775 Fragments arranged by size: 4775 882 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 253 2312 2477 2480 253 Size: 2059 165 3 3490 Fragments arranged by size: 3490 2059 165 3 BseYI C'CCAG_C Cuts at: 2363 2370 2443 2654 3142 3900 4565 4973 2363 Size: 7 73 211 488 758 665 408 3107 Fragments arranged by size: 3107 758 665 488 408 211 73 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 148 509 1636 148 Size: 361 1127 4229 Fragments arranged by size: 4229 1127 361 BsiEI CG_ry'CG Cuts at: 104 273 635 3259 3305 4535 104 Size: 169 362 2624 46 1230 1286 Fragments arranged by size: 2624 1286 1230 362 169 46 BsiHKAI G_wGCw'C Cuts at: 245 2265 2412 2731 3509 3699 4559 245 Size: 2020 147 319 778 190 860 1403 Fragments arranged by size: 2020 1403 860 778 319 190 147 BsmI GAATG_Cn' Cuts at: 1704 1704 Size: 5717 BsmBI CGTCTCn'nnnn_ Cuts at: 298 595 298 Size: 297 5420 Bsp1286I G_dGCh'C Cuts at: 245 1029 2265 2412 2731 3342 3435 3509 3699 Size: 784 1236 147 319 611 93 74 190 Cuts at: 3699 3761 4559 245 Size: 62 798 1403 Fragments arranged by size: 1403 1236 798 784 611 319 190 147 93 74 62 BspHI T'CATG_A Cuts at: 1065 4149 1065 Size: 3084 2633 BspMI ACCTGCnnnn'nnnn_ Cuts at: 2289 2700 2767 2981 3283 3664 2289 Size: 411 67 214 302 381 4342 Fragments arranged by size: 4342 411 381 302 214 67 BsrBI CCG'CTC Cuts at: 50 4009 4063 5547 5712 50 Size: 3959 54 1484 165 55 Fragments arranged by size: 3959 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 2243 3629 2243 Size: 1386 4331 BsrFI r'CCGG_y Cuts at: 3715 3896 3715 Size: 181 5536 BssHII G'CGCG_C Cuts at: 296 296 Size: 5717 BssSI C'ACGA_G Cuts at: 289 3988 4696 289 Size: 3699 708 1310 Fragments arranged by size: 3699 1310 708 BstAPI GCAn_nnn'nTGC Cuts at: 3094 3094 Size: 5717 BstBI TT'CG_AA Cuts at: 4078 4078 Size: 5717 BstXI CCAn_nnnn'nTGG Cuts at: 168 1278 2562 2629 168 Size: 1110 1284 67 3256 Fragments arranged by size: 3256 1284 1110 67 BstYI r'GATC_y Cuts at: 1 371 1089 1095 1372 2742 3209 3232 3567 Size: 370 718 6 277 1370 467 23 335 Cuts at: 3567 3813 4119 4131 4217 5269 1 Size: 246 306 12 86 1052 449 Fragments arranged by size: 1370 1052 718 467 449 370 335 306 277 246 86 23 12 6 BtgI C'CryG_G Cuts at: 107 635 2561 107 Size: 528 1926 3263 Fragments arranged by size: 3263 1926 528 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 3667 3848 4011 3667 Size: 181 163 5373 Fragments arranged by size: 5373 181 163 BtsI GCAGTG_nn' Cuts at: 1749 2187 2960 1749 Size: 438 773 4506 Fragments arranged by size: 4506 773 438 DraI TTT'AAA Cuts at: 710 2716 710 Size: 2006 3711 DrdI GACnn_nn'nnGTC Cuts at: 3423 4767 3423 Size: 1344 4373 EaeI y'GGCC_r Cuts at: 158 2586 3302 3476 3867 3894 5239 158 Size: 2428 716 174 391 27 1345 636 Fragments arranged by size: 2428 1345 716 636 391 174 27 EagI C'GGCC_G Cuts at: 3302 3302 Size: 5717 EarI CTCTTCn'nnn_ Cuts at: 203 3740 3950 203 Size: 3537 210 1970 Fragments arranged by size: 3537 1970 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 250 3925 4667 4813 5527 250 Size: 3675 742 146 714 440 Fragments arranged by size: 3675 742 714 440 146 EcoICRI GAG'CTC Cuts at: 243 2410 2729 243 Size: 2167 319 3231 Fragments arranged by size: 3231 2167 319 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 632 1355 1575 1834 2315 2417 3542 3974 4327 Size: 723 220 259 481 102 1125 432 353 Cuts at: 4327 4989 632 Size: 662 1360 Fragments arranged by size: 1360 1125 723 662 481 432 353 259 220 102 EcoO109I rG'GnC_Cy Cuts at: 662 2650 2856 662 Size: 1988 206 3523 Fragments arranged by size: 3523 1988 206 EcoRI G'AATT_C Cuts at: 2721 2721 Size: 5717 EcoRV GAT'ATC Cuts at: 2533 2533 Size: 5717 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 433 1132 1918 3736 433 Size: 699 786 1818 2414 Fragments arranged by size: 2414 1818 786 699 FspI TGC'GCA Cuts at: 3498 3498 Size: 5717 HaeII r_GCGC'y Cuts at: 2338 3151 3399 4629 5400 2338 Size: 813 248 1230 771 2655 Fragments arranged by size: 2655 1230 813 771 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 533 565 654 686 1392 1424 1813 1845 3559 Size: 32 89 32 706 32 389 32 1714 Cuts at: 3559 3589 3591 3621 533 Size: 30 2 30 2629 Fragments arranged by size: 2629 1714 706 389 89 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 1242 2147 2756 1242 Size: 905 609 4203 Fragments arranged by size: 4203 905 609 HindIII A'AGCT_T Cuts at: 450 450 Size: 5717 HpaI GTT'AAC Cuts at: 1242 1242 Size: 5717 KasI G'GCGC_C Cuts at: 3147 3395 3147 Size: 248 5469 KpnI G_GTAC'C Cuts at: 2737 2737 Size: 5717 MfeI C'AATT_G Cuts at: 483 1250 1319 483 Size: 767 69 4881 Fragments arranged by size: 4881 767 69 MluI A'CGCG_T Cuts at: 5150 5150 Size: 5717 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1460 2350 4476 4660 1460 Size: 890 2126 184 2517 Fragments arranged by size: 2517 2126 890 184 MscI TGG'CCA Cuts at: 160 2588 3478 160 Size: 2428 890 2399 Fragments arranged by size: 2428 2399 890 MslI CAynn'nnrTG Cuts at: 124 1117 1666 2560 3706 3833 124 Size: 993 549 894 1146 127 2008 Fragments arranged by size: 2008 1146 993 894 549 127 MspA1I CmG'CkG Cuts at: 132 168 279 2828 3142 3502 4286 4531 132 Size: 36 111 2549 314 360 784 245 1318 Fragments arranged by size: 2549 1318 784 360 314 245 111 36 NaeI GCC'GGC Cuts at: 3898 3898 Size: 5717 NarI GG'CG_CC Cuts at: 3148 3396 3148 Size: 248 5469 NcoI C'CATG_G Cuts at: 107 2561 107 Size: 2454 3263 NdeI CA'TA_TG Cuts at: 2085 2085 Size: 5717 NgoMIV G'CCGG_C Cuts at: 3896 3896 Size: 5717 NheI G'CTAG_C Cuts at: 499 1293 499 Size: 794 4923 NspI r_CATG'y Cuts at: 946 2314 2770 3270 4873 946 Size: 1368 456 500 1603 1790 Fragments arranged by size: 1790 1603 1368 500 456 PciI A'CATG_T Cuts at: 4869 4869 Size: 5717 PfoI T'CCnGG_A Cuts at: 2526 2628 2526 Size: 102 5615 PmeI GTTT'AAAC Cuts at: 2716 2716 Size: 5717 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4163 4195 4163 Size: 32 5685 PpuMI rG'GwC_Cy Cuts at: 662 2650 662 Size: 1988 3729 PstI C_TGCA'G Cuts at: 771 1038 2764 3449 771 Size: 267 1726 685 3039 Fragments arranged by size: 3039 1726 685 267 PvuI CG_AT'CG Cuts at: 104 104 Size: 5717 PvuII CAG'CTG Cuts at: 132 3142 3502 132 Size: 3010 360 2347 Fragments arranged by size: 3010 2347 360 RsrII CG'GwC_CG Cuts at: 3912 3912 Size: 5717 SacI G_AGCT'C Cuts at: 245 2412 2731 245 Size: 2167 319 3231 Fragments arranged by size: 3231 2167 319 SalI G'TCGA_C Cuts at: 2754 2754 Size: 5717 SanDI GG'GwC_CC Cuts at: 662 2650 662 Size: 1988 3729 SapI GCTCTTCn'nnn_ Cuts at: 203 3740 3950 203 Size: 3537 210 1970 Fragments arranged by size: 3537 1970 210 SbfI CC_TGCA'GG Cuts at: 2764 2764 Size: 5717 ScaI AGT'ACT Cuts at: 223 5234 223 Size: 5011 706 SfcI C'TryA_G Cuts at: 33 767 1034 2760 3445 4413 4604 33 Size: 734 267 1726 685 968 191 1146 Fragments arranged by size: 1726 1146 968 734 685 267 191 SfoI GGC'GCC Cuts at: 3149 3397 3149 Size: 248 5469 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5717 SmaI CCC'GGG Cuts at: 2648 2739 2648 Size: 91 5626 SmlI C'TyrA_G Cuts at: 301 618 692 1874 2049 2872 3787 4501 4763 Size: 317 74 1182 175 823 915 714 262 Cuts at: 4763 301 Size: 1255 Fragments arranged by size: 1255 1182 915 823 714 317 262 175 74 SpeI A'CTAG_T Cuts at: 67 67 Size: 5717 SphI G_CATG'C Cuts at: 946 2770 3270 946 Size: 1824 500 3393 Fragments arranged by size: 3393 1824 500 SspI AAT'ATT Cuts at: 1992 1992 Size: 5717 StuI AGG'CCT Cuts at: 2379 2379 Size: 5717 StyI C'CwwG_G Cuts at: 107 821 2561 2851 3205 107 Size: 714 1740 290 354 2619 Fragments arranged by size: 2619 1740 714 354 290 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 259 3300 3433 3892 4101 259 Size: 3041 133 459 209 1875 Fragments arranged by size: 3041 1875 459 209 133 TatI w'GTAC_w Cuts at: 221 1194 5232 221 Size: 973 4038 706 Fragments arranged by size: 4038 973 706 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5234 5266 5234 Size: 32 5685 Tth111I GACn'n_nGTC Cuts at: 3514 3514 Size: 5717 XbaI T'CTAG_A Cuts at: 1092 2748 1092 Size: 1656 4061 XcmI CCAnnnn_n'nnnnTGG Cuts at: 574 3212 574 Size: 2638 3079 XhoI C'TCGA_G Cuts at: 301 301 Size: 5717 XmaI C'CCGG_G Cuts at: 2646 2737 2646 Size: 91 5626 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflII AflIII AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NspI PciI PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI Enzymes that do not cut: AatII AfeI AgeI ApaI AscI AvrII BcgI BfrBI BglI BmgBI BsiWI BspEI BsrGI BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI FseI FspAI NotI NruI NsiI PacI PasI PflMI PmlI PshAI PsiI PspOMI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SwaI XmnI ZraI