(Linear) (Six-base) MAPSORT of: KIAA1315.seq Check: 1044 from: 1 to: 5943 KIAA1315.orf With 182 enzymes: SgfI * July 18, 2007 13:35 .. AccI GT'mk_AC Cuts at: 0 1004 4234 5943 Size: 1004 3230 1709 Fragments arranged by size: 3230 1709 1004 AclI AA'CG_TT Cuts at: 0 1888 5943 Size: 1888 4055 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 209 1427 1610 2249 2431 4157 4369 4427 Size: 209 1218 183 639 182 1726 212 58 Cuts at: 4427 4883 5633 5943 Size: 456 750 310 Fragments arranged by size: 1726 1218 750 639 456 310 212 209 183 182 58 AfeI AGC'GCT Cuts at: 0 129 3754 5943 Size: 129 3625 2189 Fragments arranged by size: 3625 2189 129 AflII C'TTAA_G Cuts at: 0 64 331 795 3319 3436 4045 5943 Size: 64 267 464 2524 117 609 1898 Fragments arranged by size: 2524 1898 609 464 267 117 64 AflIII A'CryG_T Cuts at: 0 2770 2958 3119 3141 5943 Size: 2770 188 161 22 2802 Fragments arranged by size: 2802 2770 188 161 22 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 598 5943 Size: 598 5345 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 3727 3759 5943 Size: 3727 32 2184 Fragments arranged by size: 3727 2184 32 AlwNI CAG_nnn'CTG Cuts at: 0 931 1924 2131 5074 5943 Size: 931 993 207 2943 869 Fragments arranged by size: 2943 993 931 869 207 ApoI r'AATT_y Cuts at: 0 300 2353 2676 2778 2859 3163 3175 3705 Size: 300 2053 323 102 81 304 12 530 Cuts at: 3705 4882 5095 5804 5943 Size: 1177 213 709 139 Fragments arranged by size: 2053 1177 709 530 323 304 300 213 139 102 81 12 AseI AT'TA_AT Cuts at: 0 2108 3184 5943 Size: 2108 1076 2759 Fragments arranged by size: 2759 2108 1076 BanII G_rGCy'C Cuts at: 0 2018 4849 5943 Size: 2018 2831 1094 Fragments arranged by size: 2831 2018 1094 BbsI GAAGACnn'nnnn_ Cuts at: 0 1501 1778 1967 3605 4163 5282 5943 Size: 1501 277 189 1638 558 1119 661 Fragments arranged by size: 1638 1501 1119 661 558 277 189 BciVI GTATCCnnnnn_n' Cuts at: 0 2181 5943 Size: 2181 3762 BclI T'GATC_A Cuts at: 0 531 1104 4614 5124 5943 Size: 531 573 3510 510 819 Fragments arranged by size: 3510 819 573 531 510 BfrBI ATG'CAT Cuts at: 0 1362 2004 4462 4587 5360 5943 Size: 1362 642 2458 125 773 583 Fragments arranged by size: 2458 1362 773 642 583 125 BglII A'GATC_T Cuts at: 0 486 499 1150 3016 4314 5052 5943 Size: 486 13 651 1866 1298 738 891 Fragments arranged by size: 1866 1298 891 738 651 486 13 BmgBI CAC'GTC Cuts at: 0 2914 4639 5943 Size: 2914 1725 1304 Fragments arranged by size: 2914 1725 1304 BmrI ACTGGGnnnn_n' Cuts at: 0 5396 5943 Size: 5396 547 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 729 5943 Size: 729 5214 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1001 1164 2428 3654 4004 4510 4538 5162 Size: 1001 163 1264 1226 350 506 28 624 Cuts at: 5162 5943 Size: 781 Fragments arranged by size: 1264 1226 1001 781 624 506 350 163 28 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2615 4468 5943 Size: 2615 1853 1475 Fragments arranged by size: 2615 1853 1475 BsaI GGTCTCn'nnnn_ Cuts at: 0 967 2544 2586 5895 5943 Size: 967 1577 42 3309 48 Fragments arranged by size: 3309 1577 967 48 42 BsaAI yAC'GTr Cuts at: 0 5049 5943 Size: 5049 894 BsaBI GATnn'nnATC Cuts at: 0 566 1299 1939 4361 5522 5876 5943 Size: 566 733 640 2422 1161 354 67 Fragments arranged by size: 2422 1161 733 640 566 354 67 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1683 1713 5382 5412 5943 Size: 1683 30 3669 30 531 Fragments arranged by size: 3669 1683 531 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 50 5943 Size: 50 5893 BseYI C'CCAG_C Cuts at: 0 100 3833 5943 Size: 100 3733 2110 Fragments arranged by size: 3733 2110 100 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 648 5943 Size: 648 5295 BsiHKAI G_wGCw'C Cuts at: 0 3055 4425 4849 5166 5943 Size: 3055 1370 424 317 777 Fragments arranged by size: 3055 1370 777 424 317 BsmI GAATG_Cn' Cuts at: 0 3522 5943 Size: 3522 2421 Bsp1286I G_dGCh'C Cuts at: 0 2018 3055 4425 4849 5166 5943 Size: 2018 1037 1370 424 317 777 Fragments arranged by size: 2018 1370 1037 777 424 317 BspHI T'CATG_A Cuts at: 0 2505 4833 5943 Size: 2505 2328 1110 Fragments arranged by size: 2505 2328 1110 BsrDI GCAATG_nn' Cuts at: 0 1622 4183 5943 Size: 1622 2561 1760 Fragments arranged by size: 2561 1760 1622 BsrFI r'CCGG_y Cuts at: 0 4188 5943 Size: 4188 1755 BsrGI T'GTAC_A Cuts at: 0 564 2755 4069 5943 Size: 564 2191 1314 1874 Fragments arranged by size: 2191 1874 1314 564 BstAPI GCAn_nnn'nTGC Cuts at: 0 4289 5716 5943 Size: 4289 1427 227 Fragments arranged by size: 4289 1427 227 BstBI TT'CG_AA Cuts at: 0 438 907 5943 Size: 438 469 5036 Fragments arranged by size: 5036 469 438 BstXI CCAn_nnnn'nTGG Cuts at: 0 5088 5943 Size: 5088 855 BstYI r'GATC_y Cuts at: 0 179 486 499 1150 3016 4314 5052 5064 Size: 179 307 13 651 1866 1298 738 12 Cuts at: 5064 5664 5943 Size: 600 279 Fragments arranged by size: 1866 1298 738 651 600 307 279 179 13 12 BtgI C'CryG_G Cuts at: 0 1868 5943 Size: 1868 4075 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 4325 5943 Size: 4325 1618 BtsI GCAGTG_nn' Cuts at: 0 580 660 1925 3984 5943 Size: 580 80 1265 2059 1959 Fragments arranged by size: 2059 1959 1265 580 80 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1746 1781 5943 Size: 1746 35 4162 Fragments arranged by size: 4162 1746 35 DraI TTT'AAA Cuts at: 0 282 915 1385 1532 3942 4376 4776 5153 Size: 282 633 470 147 2410 434 400 377 Cuts at: 5153 5354 5841 5943 Size: 201 487 102 Fragments arranged by size: 2410 633 487 470 434 400 377 282 201 147 102 DraIII CAC_nnn'GTG Cuts at: 0 2914 5943 Size: 2914 3029 EarI CTCTTCn'nnn_ Cuts at: 0 170 726 963 1220 1227 1916 2006 3102 Size: 170 556 237 257 7 689 90 1096 Cuts at: 3102 3468 3743 4073 4448 4662 5943 Size: 366 275 330 375 214 1281 Fragments arranged by size: 1281 1096 689 556 375 366 330 275 257 237 214 170 90 7 EcoICRI GAG'CTC Cuts at: 0 4847 5943 Size: 4847 1096 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 209 1001 1164 1427 1610 2249 2428 2431 Size: 209 792 163 263 183 639 179 3 Cuts at: 2431 3654 4004 4157 4369 4427 4510 4538 4883 Size: 1223 350 153 212 58 83 28 345 Cuts at: 4883 5162 5633 5943 Size: 279 471 310 Fragments arranged by size: 1223 792 639 471 350 345 310 279 263 212 209 183 179 163 153 83 58 28 3 EcoO109I rG'GnC_Cy Cuts at: 0 1656 5646 5943 Size: 1656 3990 297 Fragments arranged by size: 3990 1656 297 EcoRI G'AATT_C Cuts at: 0 2778 5095 5943 Size: 2778 2317 848 Fragments arranged by size: 2778 2317 848 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 647 3462 3511 4541 5943 Size: 647 2815 49 1030 1402 Fragments arranged by size: 2815 1402 1030 647 49 HaeII r_GCGC'y Cuts at: 0 131 3756 5943 Size: 131 3625 2187 Fragments arranged by size: 3625 2187 131 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 67 99 478 510 1768 1800 2380 2412 Size: 67 32 379 32 1258 32 580 32 Cuts at: 2412 2503 2535 4120 4152 4375 4407 4447 4479 Size: 91 32 1585 32 223 32 40 32 Cuts at: 4479 5419 5431 5451 5463 5649 5656 5681 5688 Size: 940 12 20 12 186 7 25 7 Cuts at: 5688 5756 5788 5943 Size: 68 32 155 Fragments arranged by size: 1585 1258 940 580 379 223 186 155 91 68 67 40 32 32 32 32 32 32 32 32 32 25 20 12 12 7 7 HincII GTy'rAC Cuts at: 0 250 1886 1992 3984 4829 5943 Size: 250 1636 106 1992 845 1114 Fragments arranged by size: 1992 1636 1114 845 250 106 HindIII A'AGCT_T Cuts at: 0 3433 3465 5943 Size: 3433 32 2478 Fragments arranged by size: 3433 2478 32 HpaI GTT'AAC Cuts at: 0 3984 5943 Size: 3984 1959 MfeI C'AATT_G Cuts at: 0 2300 5943 Size: 2300 3643 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 86 3584 4187 4826 5849 5943 Size: 86 3498 603 639 1023 94 Fragments arranged by size: 3498 1023 639 603 94 86 MslI CAynn'nnrTG Cuts at: 0 512 2510 2660 3118 4199 4430 4919 5943 Size: 512 1998 150 458 1081 231 489 1024 Fragments arranged by size: 1998 1081 1024 512 489 458 231 150 MspA1I CmG'CkG Cuts at: 0 1852 2881 4032 5943 Size: 1852 1029 1151 1911 Fragments arranged by size: 1911 1852 1151 1029 NcoI C'CATG_G Cuts at: 0 1868 5943 Size: 1868 4075 NdeI CA'TA_TG Cuts at: 0 1812 5943 Size: 1812 4131 NsiI A_TGCA'T Cuts at: 0 1364 2006 4464 4589 5362 5943 Size: 1364 642 2458 125 773 581 Fragments arranged by size: 2458 1364 773 642 581 125 NspI r_CATG'y Cuts at: 0 2774 2962 3123 3145 3841 4591 5943 Size: 2774 188 161 22 696 750 1352 Fragments arranged by size: 2774 1352 750 696 188 161 22 PacI TTA_AT'TAA Cuts at: 0 3239 5943 Size: 3239 2704 PasI CC'CwG_GG Cuts at: 0 5689 5943 Size: 5689 254 PciI A'CATG_T Cuts at: 0 2770 2958 3119 3141 5943 Size: 2770 188 161 22 2802 Fragments arranged by size: 2802 2770 188 161 22 PflMI CCAn_nnn'nTGG Cuts at: 0 2475 2914 5943 Size: 2475 439 3029 Fragments arranged by size: 3029 2475 439 PfoI T'CCnGG_A Cuts at: 0 4531 5943 Size: 4531 1412 PpuMI rG'GwC_Cy Cuts at: 0 1656 5943 Size: 1656 4287 PsiI TTA'TAA Cuts at: 0 3614 5943 Size: 3614 2329 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 2591 2623 3353 3385 5943 Size: 2591 32 730 32 2558 Fragments arranged by size: 2591 2558 730 32 32 PvuII CAG'CTG Cuts at: 0 1852 2881 4032 5943 Size: 1852 1029 1151 1911 Fragments arranged by size: 1911 1852 1151 1029 SacI G_AGCT'C Cuts at: 0 4849 5943 Size: 4849 1094 SapI GCTCTTCn'nnn_ Cuts at: 0 1916 2006 3743 4073 5943 Size: 1916 90 1737 330 1870 Fragments arranged by size: 1916 1870 1737 330 90 ScaI AGT'ACT Cuts at: 0 630 801 2925 5943 Size: 630 171 2124 3018 Fragments arranged by size: 3018 2124 630 171 SfcI C'TryA_G Cuts at: 0 1478 2597 5198 5943 Size: 1478 1119 2601 745 Fragments arranged by size: 2601 1478 1119 745 SmlI C'TyrA_G Cuts at: 0 64 331 795 2630 3319 3436 4045 4447 Size: 64 267 464 1835 689 117 609 402 Cuts at: 4447 5943 Size: 1496 Fragments arranged by size: 1835 1496 689 609 464 402 267 117 64 SnaBI TAC'GTA Cuts at: 0 5049 5943 Size: 5049 894 SphI G_CATG'C Cuts at: 0 3841 4591 5943 Size: 3841 750 1352 Fragments arranged by size: 3841 1352 750 SspI AAT'ATT Cuts at: 0 342 3938 5171 5943 Size: 342 3596 1233 772 Fragments arranged by size: 3596 1233 772 342 StuI AGG'CCT Cuts at: 0 5349 5943 Size: 5349 594 StyI C'CwwG_G Cuts at: 0 823 1868 3758 5943 Size: 823 1045 1890 2185 Fragments arranged by size: 2185 1890 1045 823 SwaI ATTT'AAAT Cuts at: 0 5153 5943 Size: 5153 790 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 3919 5943 Size: 3919 2024 TatI w'GTAC_w Cuts at: 0 564 628 799 2755 2923 3765 3988 4069 Size: 564 64 171 1956 168 842 223 81 Cuts at: 4069 5943 Size: 1874 Fragments arranged by size: 1956 1874 842 564 223 171 168 81 64 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 5276 5308 5382 5414 5943 Size: 5276 32 74 32 529 Fragments arranged by size: 5276 529 74 32 32 XbaI T'CTAG_A Cuts at: 0 496 1221 2433 5943 Size: 496 725 1212 3510 Fragments arranged by size: 3510 1212 725 496 XmnI GAAnn'nnTTC Cuts at: 0 5033 5511 5943 Size: 5033 478 432 Fragments arranged by size: 5033 478 432 Enzymes that do cut: AccI AclI AcuI AfeI AflII AflIII AlfI AloI AlwNI ApoI AseI BanII BbsI BciVI BclI BfrBI BglII BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BsrDI BsrFI BsrGI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI CspCI DraI DraIII EarI EcoICRI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII HpaI MfeI MmeI MslI MspA1I NcoI NdeI NsiI NspI PacI PasI PciI PflMI PfoI PpuMI PsiI PsrI PvuII SacI SapI ScaI SfcI SmlI SnaBI SphI SspI StuI StyI SwaI TaqII TatI TstI XbaI XmnI Enzymes that do not cut: AarI AatII Acc65I AgeI AhdI AleI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI BglI BlpI Bme1580I BmtI Bpu10I BsaHI BsaWI BsiEI BsiWI BsmBI BspEI BspMI BsrBI BssHII BssSI BstEII BstZ17I Bsu36I ClaI DrdI EaeI EagI EciI EcoNI EcoRV FseI FspI FspAI KasI KpnI MluI MscI NaeI NarI NgoMIV NheI NotI NruI PmeI PmlI PpiI PshAI PspOMI PspXI PstI PvuI RsrII SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SpeI SrfI Tth111I XcmI XhoI XmaI ZraI