Restriction map for pF1KA1313
##################################
# NO CUT ENZYMES (   61 enzymes) #
##################################

AflII       AgeI        AhdI        Alw44I      AocI        
ApaLI       AscI        AspEI       AviII       AvrII       
BamHI       BcgI        BciVI       BfrI        BlnI        
BsiWI       Bsp1407I    BspDI       BspLU11I    BsrGI       
Bst1107I    BstZ17I     Bsu36I      ClaI        CpoI        
DraI        EagI        Eam1105I    EclXI       Eco52I      
Eco81I      EcoNI       FseI        FspI        HpaI        
MfeI        MluI        MunI        NaeI        NdeI        
NgoMIV      NheI        NotI        PacI        PinAI       
PmeI        PvuI        PvuII       RsrII       SalI        
SbfI        SfiI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
XbaI        


###################################
# ONE CUT ENZYMES (   63 enzymes) #
###################################

AatII        1663  GACGTC               GACGT^C
Acc65I       2223  GGTACC               G^GTACC
AccIII       2129  TCCGGA               T^CCGGA
AclI         2257  AACGTT               AA^CGTT
AcsI         1588  GAATTC               [GA]^AATT[CT]
Aor51HI       454  AGCGCT               AGC^GCT
ApoI         1588  GAATTC               [GA]^AATT[CT]
AseI           67  ATTAAT               AT^TAAT
AsnI           67  ATTAAT               AT^TAAT
Asp700I      2716  GAAGGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      2223  GGTACC               G^GTACC
BbeI         1405  GGCGCC               GGCGC^C
BbrPI         928  CACGTG               CAC^GTG
BbvCI        2938  GCTGAGG              CCTCAGC(-5/-2)
BglI          118  GCCAACGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         410  AGATCT               A^GATCT
BlpI         3190  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     3190  GCTGAGC              GC^T[ACGT]AGC
BsaBI        2701  GATCATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         1447  GGTCTC               GGTCTC(1/5)
BsaWI        2129  TCCGGA               [AT]^CCGG[AT]
BseAI        2129  TCCGGA               T^CCGGA
BsmBI         595  GAGACG               CGTCTC(1/5)
BsmI         2737  GAATGC               GAATGC(1/-1)
BspEI        2129  TCCGGA               T^CCGGA
BspHI        2703  TCATGA               T^CATGA
BsrBI        2337  CCGCTC               CCGCTC(-3/-3)
BssHII         99  GCGCGC               G^CGCGC
BstAPI       2313  GCAGACGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        1585  TTCGAA               TT^CGAA
CelII        3190  GCTGAGC              GC^T[ACGT]AGC
DraIII        208  CACCTAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1495  GACATGCCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      454  AGCGCT               AGC^GCT
EcoRI        1588  GAATTC               G^AATTC
EcoRV        3292  GATATC               GAT^ATC
EcoT22I      3223  ATGCAT               ATGCA^T
HindIII       477  AAGCTT               A^AGCTT
KasI         1405  GGCGCC               G^GCGCC
KpnI         2223  GGTACC               GGTAC^C
KspI         1833  CCGCGG               CCGC^GG
MamI         2701  GATCATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         2129  TCCGGA               T^CCGGA
MslI         2667  CACCAGTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI         1405  GGCGCC               GG^CGCC
NcoI         2681  CCATGG               C^CATGG
NsiI         3223  ATGCAT               ATGCA^T
NspV         1585  TTCGAA               TT^CGAA
PaeR7I        307  CTCGAG               C^TCGAG
PflMI          38  CCATACTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI         928  CACGTG               CAC^GTG
PmlI          928  CACGTG               CAC^GTG
PpuMI         359  AGGACCT              [GA]G^G[AT]CC[CT]
PshBI          67  ATTAAT               AT^TAAT
Psp1406I     2257  AACGTT               AA^CGTT
RcaI         2703  TCATGA               T^CATGA
SacII        1833  CCGCGG               CCGC^GG
SapI         2270  GCTCTTC              GCTCTTC(1/4)
SfoI         1405  GGCGCC               GGC^GCC
SfuI         1585  TTCGAA               TT^CGAA
Van91I         38  CCATACTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          307  CTCGAG               C^TCGAG
XmnI         2716  GAAGGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AccI          820  GTCTAC               GT^[AC][GT]AC
             1723  GTCTAC              
AcyI         1405  GGCGCC               G[GA]^CG[CT]C
             1663  GACGTC              
AflIII        929  ACGTGT               A^C[GA][CT]GT
             1146  ACGTGT              
AlwNI        2314  CAGACGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2763  CAGGTGCTG           
ApaI          964  GGGCCC               GGGCC^C
             2428  GGGCCC              
AspI          597  GACGCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1852  GACCAGGTC           
BbsI         1777  GAAGAC               GAAGAC(2/6)
             1504  GTCTTC              
BclI         2700  TGATCA               T^GATCA
             2706  TGATCA              
BfaI          211  CTAG                 C^TAG
              631  CTAG                
BpuAI        1777  GAAGAC               GAAGAC(2/6)
             1504  GTCTTC              
BsaHI        1405  GGCGCC               G[GA]^CG[CT]C
             1663  GACGTC              
BsiEI         846  CGACCG               CG[GA][CT]^CG
             1830  CGACCG              
BspMI        1040  ACCTGC               ACCTGC(4/8)
             1485  GCAGGT              
BsrFI          18  GCCGGT               [GA]^CCGG[CT]
             1029  GCCGGT              
BssSI        1567  CACGAG               CACGAG(-5/-1)
              568  CTCGTG              
Cfr10I         18  GCCGGT               [GA]^CCGG[CT]
             1029  GCCGGT              
FbaI         2700  TGATCA               T^GATCA
             2706  TGATCA              
Hin1I        1405  GGCGCC               G[GA]^CG[CT]C
             1663  GACGTC              
MaeI          211  CTAG                 C^TAG
              631  CTAG                
NruI         1125  TCGCGA               TCG^CGA
             1431  TCGCGA              
NspI         1496  ACATGC               [GA]CATG^[CT]
             2814  GCATGT              
PflFI         597  GACGCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1852  GACCAGGTC           
PshAI        1441  GACCTGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             1761  GACTGTTGTC          
ScaI           77  AGTACT               AGT^ACT
             2156  AGTACT              
SmaI         1089  CCCGGG               CCC^GGG
             2228  CCCGGG              
StuI         1134  AGGCCT               AGG^CCT
             2947  AGGCCT              
TfiI          620  GAATC                G^A[AT]TC
             2745  GATTC               
Tth111I       597  GACGCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1852  GACCAGGTC           
XcmI          806  CCAACGGCCAGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1208  CCACTATTCTGGTGG     
XmaCI        1089  CCCGGG               C^CCGGG
             2228  CCCGGG              
XmaI         1089  CCCGGG               C^CCGGG
             2228  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  103 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AspHI       AvaI        AvaII       BalI        BanI        
BanII       BbvI        BcnI        BmyI        BpmI        
BsaAI       BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BsrDI       BsrI        BssKI       BstEII      
BstF5I      BstNI       BstPI       BstUI       BstXI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
EcoO109I    EcoO65I     EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        PstI        
RsaI        SacI        Sau3AI      Sau96I      ScrFI       
SexAI       SfaNI       SfcI        SmlI        StyI        
TaqI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII       
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