(Circular) (Six-base) MAPSORT of: pF1KA1313.seq Check: 3039 from: 1 to: 6416 pF1KA1313 6416 bp With 182 enzymes: SgfI * June 20, 2008 10:21 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 1584 1584 Size: 6416 AatII G_ACGT'C Cuts at: 1775 1775 Size: 6416 AccI GT'mk_AC Cuts at: 929 1832 3454 929 Size: 903 1622 3891 Fragments arranged by size: 3891 1622 903 Acc65I G'GTAC_C Cuts at: 2331 3432 2331 Size: 1101 5315 AclI AA'CG_TT Cuts at: 2366 6256 2366 Size: 3890 2526 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 266 2698 3314 3373 3415 4241 4673 5026 5688 Size: 2432 616 59 42 826 432 353 662 Cuts at: 5688 266 Size: 994 Fragments arranged by size: 2432 994 826 662 616 432 353 59 42 AfeI AGC'GCT Cuts at: 564 564 Size: 6416 AflIII A'CryG_T Cuts at: 1037 1254 3768 5568 5849 1037 Size: 217 2514 1800 281 1604 Fragments arranged by size: 2514 1800 1604 281 217 AleI CACnn'nnGTG Cuts at: 2779 2779 Size: 6416 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 2229 2229 Size: 6416 AlwNI CAG_nnn'CTG Cuts at: 2427 2876 3721 5159 2427 Size: 449 845 1438 3684 Fragments arranged by size: 3684 1438 845 449 ApaI G_GGCC'C Cuts at: 1076 2540 1076 Size: 1464 4952 ApaLI G'TGCA_C Cuts at: 5254 5254 Size: 6416 ApoI r'AATT_y Cuts at: 1696 3420 5769 6025 1696 Size: 1724 2349 256 2087 Fragments arranged by size: 2349 2087 1724 256 AseI AT'TA_AT Cuts at: 20 176 20 Size: 156 6260 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 6416 AvaI C'yCGr_G Cuts at: 415 1197 2336 3436 415 Size: 782 1139 1100 3395 Fragments arranged by size: 3395 1139 1100 782 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2741 2774 2741 Size: 33 6383 BamHI G'GATC_C Cuts at: 3441 3441 Size: 6416 BanI G'GyrC_C Cuts at: 766 910 1513 2331 3432 3846 4094 4129 766 Size: 144 603 818 1101 414 248 35 3053 Fragments arranged by size: 3053 1101 818 603 414 248 144 35 BanII G_rGCy'C Cuts at: 397 611 971 1076 2008 2128 2167 2540 2628 Size: 214 360 105 932 120 39 373 88 Cuts at: 2628 3187 3305 3430 4460 397 Size: 559 118 125 1030 2353 Fragments arranged by size: 2353 1030 932 559 373 360 214 125 120 118 105 88 39 BbeI G_GCGC'C Cuts at: 1517 3850 4098 1517 Size: 2333 248 3835 Fragments arranged by size: 3835 2333 248 BbsI GAAGACnn'nnnn_ Cuts at: 1605 1892 1605 Size: 287 6129 BbvCI CC'TCA_GC Cuts at: 3047 3047 Size: 6416 BciVI GTATCCnnnnn_n' Cuts at: 4314 5370 6416 4314 Size: 1056 1046 4314 Fragments arranged by size: 4314 1056 1046 BclI T'GATC_A Cuts at: 2808 2814 3936 2808 Size: 6 1122 5288 Fragments arranged by size: 5288 1122 6 BfrBI ATG'CAT Cuts at: 3333 3333 Size: 6416 BglI GCCn_nnn'nGGC Cuts at: 232 232 Size: 6416 BglII A'GATC_T Cuts at: 518 3931 5968 518 Size: 3413 2037 966 Fragments arranged by size: 3413 2037 966 BlpI GC'TnA_GC Cuts at: 3299 3528 3299 Size: 229 6187 Bme1580I G_kGCm'C Cuts at: 769 913 1037 1076 1286 1400 2540 4041 4134 Size: 144 124 39 210 114 1140 1501 93 Cuts at: 4134 5258 769 Size: 1124 1927 Fragments arranged by size: 1927 1501 1140 1124 210 144 124 114 93 39 BmgBI CAC'GTC Cuts at: 1053 1255 1053 Size: 202 6214 BmrI ACTGGGnnnn_n' Cuts at: 482 3628 3709 4044 6188 482 Size: 3146 81 335 2144 710 Fragments arranged by size: 3146 2144 710 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1329 4783 1329 Size: 3454 2962 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 535 655 3268 535 Size: 120 2613 3683 Fragments arranged by size: 3683 2613 120 Bpu10I CC'TnA_GC Cuts at: 1061 2918 3047 1061 Size: 1857 129 4430 Fragments arranged by size: 4430 1857 129 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 166 3163 3592 4471 5185 5483 166 Size: 2997 429 879 714 298 1099 Fragments arranged by size: 2997 1099 879 714 429 298 BsaI GGTCTCn'nnnn_ Cuts at: 1561 6120 1561 Size: 4559 1857 BsaAI yAC'GTr Cuts at: 1038 1206 2613 3769 1038 Size: 168 1407 1156 3685 Fragments arranged by size: 3685 1407 1156 168 BsaBI GATnn'nnATC Cuts at: 6 2813 3930 5967 6 Size: 2807 1117 2037 455 Fragments arranged by size: 2807 2037 1117 455 BsaHI Gr'CG_yC Cuts at: 1514 1772 3847 4095 4797 6293 1514 Size: 258 2075 248 702 1496 1637 Fragments arranged by size: 2075 1637 1496 702 258 248 BsaWI w'CCGG_w Cuts at: 2237 3605 4126 4804 5215 5362 5636 2237 Size: 1368 521 678 411 147 274 3017 Fragments arranged by size: 3017 1368 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 214 2362 2382 2710 3064 214 Size: 2148 20 328 354 3566 Fragments arranged by size: 3566 2148 354 328 20 BseYI C'CCAG_C Cuts at: 334 1272 2540 3234 3841 4599 5264 5672 334 Size: 938 1268 694 607 758 665 408 1078 Fragments arranged by size: 1268 1078 938 758 694 665 607 408 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 616 1075 1279 1513 616 Size: 459 204 234 5519 Fragments arranged by size: 5519 459 234 204 BsiEI CG_ry'CG Cuts at: 104 957 1941 3958 4004 5234 104 Size: 853 984 2017 46 1230 1286 Fragments arranged by size: 2017 1286 1230 984 853 46 BsiHKAI G_wGCw'C Cuts at: 611 971 1433 2008 2458 3187 3430 4208 4398 Size: 360 462 575 450 729 243 778 190 Cuts at: 4398 5258 611 Size: 860 1769 Fragments arranged by size: 1769 860 778 729 575 462 450 360 243 190 BsmI GAATG_Cn' Cuts at: 2851 2851 Size: 6416 BsmBI CGTCTCn'nnnn_ Cuts at: 697 697 Size: 6416 Bsp1286I G_dGCh'C Cuts at: 397 611 769 913 971 1037 1076 1286 1400 Size: 214 158 144 58 66 39 210 114 Cuts at: 1400 1433 2008 2128 2167 2458 2540 2628 3187 Size: 33 575 120 39 291 82 88 559 Cuts at: 3187 3305 3430 4041 4134 4208 4398 4460 5258 Size: 118 125 611 93 74 190 62 798 Cuts at: 5258 397 Size: 1555 Fragments arranged by size: 1555 798 611 575 559 291 214 210 190 158 144 125 120 118 114 93 88 82 74 66 62 58 39 39 33 BspEI T'CCGG_A Cuts at: 2237 2237 Size: 6416 BspHI T'CATG_A Cuts at: 2811 4848 2811 Size: 2037 4379 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1157 1584 3466 3680 3982 4363 1157 Size: 427 1882 214 302 381 3210 Fragments arranged by size: 3210 1882 427 381 302 214 BsrBI CCG'CTC Cuts at: 50 2447 4708 4762 6246 6411 50 Size: 2397 2261 54 1484 165 55 Fragments arranged by size: 2397 2261 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1284 2168 2832 3141 3218 4328 1284 Size: 884 664 309 77 1110 3372 Fragments arranged by size: 3372 1110 884 664 309 77 BsrFI r'CCGG_y Cuts at: 126 1137 4414 4595 126 Size: 1011 3277 181 1947 Fragments arranged by size: 3277 1947 1011 181 BssHII G'CGCG_C Cuts at: 207 207 Size: 6416 BssSI C'ACGA_G Cuts at: 676 1675 4687 5395 676 Size: 999 3012 708 1697 Fragments arranged by size: 3012 1697 999 708 BstAPI GCAn_nnn'nTGC Cuts at: 2427 3793 2427 Size: 1366 5050 BstBI TT'CG_AA Cuts at: 1694 4777 1694 Size: 3083 3333 BstEII G'GTnAC_C Cuts at: 351 789 1101 351 Size: 438 312 5666 Fragments arranged by size: 5666 438 312 BstXI CCAn_nnnn'nTGG Cuts at: 435 1323 1969 3225 435 Size: 888 646 1256 3626 Fragments arranged by size: 3626 1256 888 646 BstYI r'GATC_y Cuts at: 1 518 2234 2794 3441 3908 3931 4266 4512 Size: 517 1716 560 647 467 23 335 246 Cuts at: 4512 4818 4830 4916 5968 1 Size: 306 12 86 1052 449 Fragments arranged by size: 1716 1052 647 560 517 467 449 335 306 246 86 23 12 BtgI C'CryG_G Cuts at: 107 1941 2046 2789 3306 107 Size: 1834 105 743 517 3217 Fragments arranged by size: 3217 1834 743 517 105 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2922 4366 4547 4710 2922 Size: 1444 181 163 4628 Fragments arranged by size: 4628 1444 181 163 BtsI GCAGTG_nn' Cuts at: 731 1092 1267 1572 1732 1820 3659 731 Size: 361 175 305 160 88 1839 3488 Fragments arranged by size: 3488 1839 361 305 175 160 88 DraI TTT'AAA Cuts at: 3415 3415 Size: 6416 DraIII CAC_nnn'GTG Cuts at: 321 321 Size: 6416 DrdI GACnn_nn'nnGTC Cuts at: 1609 4122 5466 1609 Size: 2513 1344 2559 Fragments arranged by size: 2559 2513 1344 EaeI y'GGCC_r Cuts at: 143 233 918 1709 1908 2859 4001 4175 4566 Size: 90 685 791 199 951 1142 174 391 Cuts at: 4566 4593 5938 143 Size: 27 1345 621 Fragments arranged by size: 1345 1142 951 791 685 621 391 199 174 90 27 EagI C'GGCC_G Cuts at: 4001 4001 Size: 6416 EarI CTCTTCn'nnn_ Cuts at: 191 1022 2385 4439 4649 191 Size: 831 1363 2054 210 1958 Fragments arranged by size: 2054 1958 1363 831 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 871 1124 2314 4624 5366 5512 6226 871 Size: 253 1190 2310 742 146 714 1061 Fragments arranged by size: 2310 1190 1061 742 714 253 146 EcoICRI GAG'CTC Cuts at: 609 969 2006 3185 3428 609 Size: 360 1037 1179 243 3597 Fragments arranged by size: 3597 1179 1037 360 243 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 266 535 655 2698 3268 3314 3373 3415 4241 Size: 269 120 2043 570 46 59 42 826 Cuts at: 4241 4673 5026 5688 266 Size: 432 353 662 994 Fragments arranged by size: 2043 994 826 662 570 432 353 269 120 59 46 42 EcoO109I rG'GnC_Cy Cuts at: 468 1072 2536 2537 3555 468 Size: 604 1464 1 1018 3329 Fragments arranged by size: 3329 1464 1018 604 1 EcoRI G'AATT_C Cuts at: 1696 3420 1696 Size: 1724 4692 EcoRV GAT'ATC Cuts at: 3402 3402 Size: 6416 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4435 4435 Size: 6416 FspI TGC'GCA Cuts at: 4197 4197 Size: 6416 HaeII r_GCGC'y Cuts at: 566 1016 1193 1517 3850 4098 5328 6099 566 Size: 450 177 324 2333 248 1230 771 883 Fragments arranged by size: 2333 1230 883 771 450 324 248 177 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 409 441 562 594 694 726 1222 1254 2793 Size: 32 121 32 100 32 496 32 1539 Cuts at: 2793 2825 4258 4288 4290 4320 409 Size: 32 1433 30 2 30 2505 Fragments arranged by size: 2505 1539 1433 496 121 100 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 951 1161 1776 2760 3111 3261 3270 3455 951 Size: 210 615 984 351 150 9 185 3912 Fragments arranged by size: 3912 984 615 351 210 185 150 9 HindIII A'AGCT_T Cuts at: 585 585 Size: 6416 KasI G'GCGC_C Cuts at: 1513 3846 4094 1513 Size: 2333 248 3835 Fragments arranged by size: 3835 2333 248 KpnI G_GTAC'C Cuts at: 2335 3436 2335 Size: 1101 5315 MluI A'CGCG_T Cuts at: 5849 5849 Size: 6416 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 326 2630 5175 5359 326 Size: 2304 2545 184 1383 Fragments arranged by size: 2545 2304 1383 184 MscI TGG'CCA Cuts at: 145 235 1711 2861 4177 145 Size: 90 1476 1150 1316 2384 Fragments arranged by size: 2384 1476 1316 1150 90 MslI CAynn'nnrTG Cuts at: 2779 4405 4532 2779 Size: 1626 127 4663 Fragments arranged by size: 4663 1626 127 MspA1I CmG'CkG Cuts at: 555 1943 2733 3527 3841 4201 4985 5230 555 Size: 1388 790 794 314 360 784 245 1741 Fragments arranged by size: 1741 1388 794 790 784 360 314 245 NaeI GCC'GGC Cuts at: 4597 4597 Size: 6416 NarI GG'CG_CC Cuts at: 1514 3847 4095 1514 Size: 2333 248 3835 Fragments arranged by size: 3835 2333 248 NcoI C'CATG_G Cuts at: 107 2789 107 Size: 2682 3734 NgoMIV G'CCGG_C Cuts at: 4595 4595 Size: 6416 NruI TCG'CGA Cuts at: 1235 1541 1235 Size: 306 6110 NsiI A_TGCA'T Cuts at: 3335 3335 Size: 6416 NspI r_CATG'y Cuts at: 1608 2926 3469 3969 5572 1608 Size: 1318 543 500 1603 2452 Fragments arranged by size: 2452 1603 1318 543 500 PasI CC'CwG_GG Cuts at: 2160 2433 2160 Size: 273 6143 PciI A'CATG_T Cuts at: 5568 5568 Size: 6416 PflMI CCAn_nnn'nTGG Cuts at: 152 152 Size: 6416 PfoI T'CCnGG_A Cuts at: 3122 3122 Size: 6416 PmeI GTTT'AAAC Cuts at: 3415 3415 Size: 6416 PmlI CAC'GTG Cuts at: 1038 1038 Size: 6416 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4862 4894 4862 Size: 32 6384 PpuMI rG'GwC_Cy Cuts at: 468 468 Size: 6416 PshAI GACnn'nnGTC Cuts at: 1553 1873 1553 Size: 320 6096 PspOMI G'GGCC_C Cuts at: 1072 2536 1072 Size: 1464 4952 PspXI vC'TCGA_Gb Cuts at: 415 415 Size: 6416 PstI C_TGCA'G Cuts at: 1298 1409 2254 3100 3463 4148 1298 Size: 111 845 846 363 685 3566 Fragments arranged by size: 3566 846 845 685 363 111 PvuI CG_AT'CG Cuts at: 104 104 Size: 6416 PvuII CAG'CTG Cuts at: 3841 4201 3841 Size: 360 6056 RsrII CG'GwC_CG Cuts at: 4611 4611 Size: 6416 SacI G_AGCT'C Cuts at: 611 971 2008 3187 3430 611 Size: 360 1037 1179 243 3597 Fragments arranged by size: 3597 1179 1037 360 243 SacII CC_GC'GG Cuts at: 1944 1944 Size: 6416 SalI G'TCGA_C Cuts at: 3453 3453 Size: 6416 SapI GCTCTTCn'nnn_ Cuts at: 2385 4439 4649 2385 Size: 2054 210 4152 Fragments arranged by size: 4152 2054 210 SbfI CC_TGCA'GG Cuts at: 3463 3463 Size: 6416 ScaI AGT'ACT Cuts at: 187 2266 5933 187 Size: 2079 3667 670 Fragments arranged by size: 3667 2079 670 SexAI A'CCwGG_T Cuts at: 1481 1862 1961 1481 Size: 381 99 5936 Fragments arranged by size: 5936 381 99 SfcI C'TryA_G Cuts at: 33 717 1294 1405 2250 3096 3459 4144 5112 Size: 684 577 111 845 846 363 685 968 Cuts at: 5112 5303 33 Size: 191 1146 Fragments arranged by size: 1146 968 846 845 685 684 577 363 191 111 SfoI GGC'GCC Cuts at: 1515 3848 4096 1515 Size: 2333 248 3835 Fragments arranged by size: 3835 2333 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 6416 SmaI CCC'GGG Cuts at: 1199 2338 3438 1199 Size: 1139 1100 4177 Fragments arranged by size: 4177 1139 1100 SmlI C'TyrA_G Cuts at: 181 415 3178 3571 4486 5200 5462 181 Size: 234 2763 393 915 714 262 1135 Fragments arranged by size: 2763 1135 915 714 393 262 234 SpeI A'CTAG_T Cuts at: 67 67 Size: 6416 SphI G_CATG'C Cuts at: 3469 3969 3469 Size: 500 5916 StuI AGG'CCT Cuts at: 1244 3057 1244 Size: 1813 4603 StyI C'CwwG_G Cuts at: 107 1214 1685 1712 2789 3104 3550 3904 107 Size: 1107 471 27 1077 315 446 354 2619 Fragments arranged by size: 2619 1107 1077 471 446 354 315 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 808 1945 3999 4132 4591 4800 808 Size: 1137 2054 133 459 209 2424 Fragments arranged by size: 2424 2054 1137 459 209 133 TatI w'GTAC_w Cuts at: 185 2264 5931 185 Size: 2079 3667 670 Fragments arranged by size: 3667 2079 670 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 863 895 5933 5965 863 Size: 32 5038 32 1314 Fragments arranged by size: 5038 1314 32 32 Tth111I GACn'n_nGTC Cuts at: 708 1963 4213 708 Size: 1255 2250 2911 Fragments arranged by size: 2911 2250 1255 XbaI T'CTAG_A Cuts at: 3447 3447 Size: 6416 XcmI CCAnnnn_n'nnnnTGG Cuts at: 921 1323 3911 921 Size: 402 2588 3426 Fragments arranged by size: 3426 2588 402 XhoI C'TCGA_G Cuts at: 415 415 Size: 6416 XmaI C'CCGG_G Cuts at: 1197 2336 3436 1197 Size: 1139 1100 4177 Fragments arranged by size: 4177 1139 1100 XmnI GAAnn'nnTTC Cuts at: 2828 2828 Size: 6416 ZraI GAC'GTC Cuts at: 1773 1773 Size: 6416 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflIII AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AflII AgeI AhdI AloI AscI AvrII BcgI BmtI BsaXI BsiWI BsrGI BstZ17I Bsu36I ClaI CspCI EcoNI FseI FspAI HpaI MfeI NdeI NheI NotI PacI PsiI PsrI SanDI SfiI SgrAI SnaBI SrfI SspI SwaI