(Circular) (Six-base) MAPSORT of: pF1KA1286.seq Check: 4105 from: 1 to: 6728 pF1KA1286 6728 bp With 182 enzymes: SgfI * June 20, 2008 10:21 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2243 3636 2243 Size: 1393 5335 AccI GT'mk_AC Cuts at: 236 671 3766 236 Size: 435 3095 3198 Fragments arranged by size: 3198 3095 435 Acc65I G'GTAC_C Cuts at: 2857 3744 2857 Size: 887 5841 AclI AA'CG_TT Cuts at: 6568 6568 Size: 6728 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 314 563 1019 1366 1774 2362 3349 3484 4553 Size: 249 456 347 408 588 987 135 1069 Cuts at: 4553 4985 5338 6000 314 Size: 432 353 662 1042 Fragments arranged by size: 1069 1042 987 662 588 456 432 408 353 347 249 135 AflIII A'CryG_T Cuts at: 1159 4080 5880 6161 1159 Size: 2921 1800 281 1726 Fragments arranged by size: 2921 1800 1726 281 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 2251 3658 2251 Size: 1407 5321 AlwNI CAG_nnn'CTG Cuts at: 2451 3047 3493 4033 5471 2451 Size: 596 446 540 1438 3708 Fragments arranged by size: 3708 1438 596 540 446 ApaI G_GGCC'C Cuts at: 720 972 2319 2397 2525 3695 720 Size: 252 1347 78 128 1170 3753 Fragments arranged by size: 3753 1347 1170 252 128 78 ApaLI G'TGCA_C Cuts at: 1163 5566 1163 Size: 4403 2325 ApoI r'AATT_y Cuts at: 1990 3732 6081 6337 1990 Size: 1742 2349 256 2381 Fragments arranged by size: 2381 2349 1742 256 AseI AT'TA_AT Cuts at: 20 2693 20 Size: 2673 4055 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 6728 AvaI C'yCGr_G Cuts at: 191 2218 2239 3236 3407 3748 191 Size: 2027 21 997 171 341 3171 Fragments arranged by size: 3171 2027 997 341 171 21 BamHI G'GATC_C Cuts at: 992 1932 3753 992 Size: 940 1821 3967 Fragments arranged by size: 3967 1821 940 BanI G'GyrC_C Cuts at: 937 1222 2857 3744 4158 4406 4441 937 Size: 285 1635 887 414 248 35 3224 Fragments arranged by size: 3224 1635 887 414 285 248 35 BanII G_rGCy'C Cuts at: 720 972 1181 1199 2319 2397 2525 2627 2675 Size: 252 209 18 1120 78 128 102 48 Cuts at: 2675 2753 3695 3742 4772 720 Size: 78 942 47 1030 2676 Fragments arranged by size: 2676 1120 1030 942 252 209 128 102 78 78 48 47 18 BbeI G_GCGC'C Cuts at: 941 4162 4410 941 Size: 3221 248 3259 Fragments arranged by size: 3259 3221 248 BbsI GAAGACnn'nnnn_ Cuts at: 551 746 1735 2264 2288 2579 3320 3590 551 Size: 195 989 529 24 291 741 270 3689 Fragments arranged by size: 3689 989 741 529 291 270 195 24 BciVI GTATCCnnnnn_n' Cuts at: 2508 4626 5682 6728 2508 Size: 2118 1056 1046 2508 Fragments arranged by size: 2508 2118 1056 1046 BclI T'GATC_A Cuts at: 3386 4248 3386 Size: 862 5866 BfrBI ATG'CAT Cuts at: 427 427 Size: 6728 BglI GCCn_nnn'nGGC Cuts at: 1220 2520 2631 1220 Size: 1300 111 5317 Fragments arranged by size: 5317 1300 111 BglII A'GATC_T Cuts at: 1565 4243 6280 1565 Size: 2678 2037 2013 Fragments arranged by size: 2678 2037 2013 BlpI GC'TnA_GC Cuts at: 1706 3840 1706 Size: 2134 4594 Bme1580I G_kGCm'C Cuts at: 635 720 965 972 1167 1442 2230 2319 2397 Size: 85 245 7 195 275 788 89 78 Cuts at: 2397 2525 3245 3695 4353 4446 5570 635 Size: 128 720 450 658 93 1124 1793 Fragments arranged by size: 1793 1124 788 720 658 450 275 245 195 128 93 89 85 78 7 BmgBI CAC'GTC Cuts at: 2286 3459 2286 Size: 1173 5555 BmrI ACTGGGnnnn_n' Cuts at: 975 2639 3940 4021 4356 6500 975 Size: 1664 1301 81 335 2144 1203 Fragments arranged by size: 2144 1664 1301 1203 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 134 1281 1846 2246 3036 3520 5095 134 Size: 1147 565 400 790 484 1575 1767 Fragments arranged by size: 1767 1575 1147 790 565 484 400 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1894 2056 2374 2566 2641 2971 2978 3515 1894 Size: 162 318 192 75 330 7 537 5107 Fragments arranged by size: 5107 537 330 318 192 162 75 7 Bpu10I CC'TnA_GC Cuts at: 3285 3676 3285 Size: 391 6337 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 160 700 1391 1414 1986 3586 3904 4783 5497 Size: 540 691 23 572 1600 318 879 714 Cuts at: 5497 5795 160 Size: 298 1093 Fragments arranged by size: 1600 1093 879 714 691 572 540 318 298 23 BsaI GGTCTCn'nnnn_ Cuts at: 190 1582 3163 3317 6432 190 Size: 1392 1581 154 3115 486 Fragments arranged by size: 3115 1581 1392 486 154 BsaAI yAC'GTr Cuts at: 4081 4081 Size: 6728 BsaBI GATnn'nnATC Cuts at: 6 4242 6279 6 Size: 4236 2037 455 Fragments arranged by size: 4236 2037 455 BsaHI Gr'CG_yC Cuts at: 249 842 938 3199 4159 4407 5109 6605 249 Size: 593 96 2261 960 248 702 1496 372 Fragments arranged by size: 2261 1496 960 702 593 372 248 96 BsaWI w'CCGG_w Cuts at: 3917 4438 5116 5527 5674 5948 3917 Size: 521 678 411 147 274 4697 Fragments arranged by size: 4697 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1495 1525 1741 1771 2257 2287 3036 3066 3331 Size: 30 216 30 486 30 749 30 265 Cuts at: 3331 3361 1495 Size: 30 4862 Fragments arranged by size: 4862 749 486 265 216 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 595 1378 1762 2077 2086 2578 2597 595 Size: 783 384 315 9 492 19 4726 Fragments arranged by size: 4726 783 492 384 315 19 9 BseYI C'CCAG_C Cuts at: 218 457 1174 3077 3468 4153 4911 5576 5984 Size: 239 717 1903 391 685 758 665 408 Cuts at: 5984 218 Size: 962 Fragments arranged by size: 1903 962 758 717 685 665 408 391 239 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1130 1757 1922 2356 2621 3406 3664 1130 Size: 627 165 434 265 785 258 4194 Fragments arranged by size: 4194 785 627 434 265 258 165 BsiEI CG_ry'CG Cuts at: 104 2855 4270 4316 5546 104 Size: 2751 1415 46 1230 1286 Fragments arranged by size: 2751 1415 1286 1230 46 BsiHKAI G_wGCw'C Cuts at: 184 1167 2753 3742 4520 4710 5570 184 Size: 983 1586 989 778 190 860 1342 Fragments arranged by size: 1586 1342 989 983 860 778 190 BsmI GAATG_Cn' Cuts at: 144 331 1147 144 Size: 187 816 5725 Fragments arranged by size: 5725 816 187 Bsp1286I G_dGCh'C Cuts at: 184 635 720 965 972 1167 1181 1199 1442 Size: 451 85 245 7 195 14 18 243 Cuts at: 1442 2230 2319 2397 2525 2627 2675 2753 3245 Size: 788 89 78 128 102 48 78 492 Cuts at: 3245 3695 3742 4353 4446 4520 4710 4772 5570 Size: 450 47 611 93 74 190 62 798 Cuts at: 5570 184 Size: 1342 Fragments arranged by size: 1342 798 788 611 492 451 450 245 243 195 190 128 102 93 89 85 78 78 74 62 48 47 18 14 7 BspHI T'CATG_A Cuts at: 1184 5160 1184 Size: 3976 2752 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1087 1833 1925 2243 2705 3636 3778 3992 4294 Size: 746 92 318 462 931 142 214 302 Cuts at: 4294 4675 1087 Size: 381 3140 Fragments arranged by size: 3140 931 746 462 381 318 302 214 142 92 BsrBI CCG'CTC Cuts at: 50 1788 5020 5074 6558 6723 50 Size: 1738 3232 54 1484 165 55 Fragments arranged by size: 3232 1738 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 792 4640 792 Size: 3848 2880 BsrFI r'CCGG_y Cuts at: 149 674 2854 4726 4907 149 Size: 525 2180 1872 181 1970 Fragments arranged by size: 2180 1970 1872 525 181 BsrGI T'GTAC_A Cuts at: 822 822 Size: 6728 BssSI C'ACGA_G Cuts at: 4999 5707 4999 Size: 708 6020 BstAPI GCAn_nnn'nTGC Cuts at: 920 2556 2799 4105 920 Size: 1636 243 1306 3543 Fragments arranged by size: 3543 1636 1306 243 BstBI TT'CG_AA Cuts at: 5089 5089 Size: 6728 BstXI CCAn_nnnn'nTGG Cuts at: 1872 2007 2736 3720 1872 Size: 135 729 984 4880 Fragments arranged by size: 4880 984 729 135 BstYI r'GATC_y Cuts at: 1 992 1565 1932 3753 4220 4243 4578 4824 Size: 991 573 367 1821 467 23 335 246 Cuts at: 4824 5130 5142 5228 6280 1 Size: 306 12 86 1052 449 Fragments arranged by size: 1821 1052 991 573 467 449 367 335 306 246 86 23 12 Bsu36I CC'TnA_GG Cuts at: 857 857 Size: 6728 BtgI C'CryG_G Cuts at: 1865 2967 3216 3318 3590 1865 Size: 1102 249 102 272 5003 Fragments arranged by size: 5003 1102 272 249 102 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1346 3679 4678 4859 5022 1346 Size: 2333 999 181 163 3052 Fragments arranged by size: 3052 2333 999 181 163 BtsI GCAGTG_nn' Cuts at: 717 1283 1745 2767 3195 3550 3971 717 Size: 566 462 1022 428 355 421 3474 Fragments arranged by size: 3474 1022 566 462 428 421 355 ClaI AT'CG_AT Cuts at: 734 3392 734 Size: 2658 4070 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 915 950 915 Size: 35 6693 DraI TTT'AAA Cuts at: 3727 3727 Size: 6728 DraIII CAC_nnn'GTG Cuts at: 1276 2869 1276 Size: 1593 5135 DrdI GACnn_nn'nnGTC Cuts at: 4434 5778 4434 Size: 1344 5384 EaeI y'GGCC_r Cuts at: 1280 2852 3031 4313 4487 4878 4905 6250 1280 Size: 1572 179 1282 174 391 27 1345 1758 Fragments arranged by size: 1758 1572 1345 1282 391 179 174 27 EagI C'GGCC_G Cuts at: 2852 4313 2852 Size: 1461 5267 EarI CTCTTCn'nnn_ Cuts at: 802 1186 1367 1373 1388 1813 2902 3005 3083 Size: 384 181 6 15 425 1089 103 78 Cuts at: 3083 3511 3523 4751 4961 802 Size: 428 12 1228 210 2569 Fragments arranged by size: 2569 1228 1089 428 425 384 210 181 103 78 15 12 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2043 2137 2401 2600 4936 5678 5824 6538 2043 Size: 94 264 199 2336 742 146 714 2233 Fragments arranged by size: 2336 2233 742 714 264 199 146 94 EcoICRI GAG'CTC Cuts at: 2751 3740 2751 Size: 989 5739 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 314 563 1019 1366 1774 1894 2056 2362 2374 Size: 249 456 347 408 120 162 306 12 Cuts at: 2374 2566 2641 2971 2978 3349 3484 3515 4553 Size: 192 75 330 7 371 135 31 1038 Cuts at: 4553 4985 5338 6000 314 Size: 432 353 662 1042 Fragments arranged by size: 1042 1038 662 456 432 408 371 353 347 330 306 249 192 162 135 120 75 31 12 7 EcoNI CCTnn'n_nnAGG Cuts at: 1551 1587 1921 1551 Size: 36 334 6358 Fragments arranged by size: 6358 334 36 EcoO109I rG'GnC_Cy Cuts at: 585 716 2157 2393 2521 2522 2946 3071 3414 Size: 131 1441 236 128 1 424 125 343 Cuts at: 3414 3691 3692 3867 585 Size: 277 1 175 3446 Fragments arranged by size: 3446 1441 424 343 277 236 175 131 128 125 1 1 EcoRI G'AATT_C Cuts at: 1990 3732 1990 Size: 1742 4986 EcoRV GAT'ATC Cuts at: 318 318 Size: 6728 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4747 4747 Size: 6728 FspI TGC'GCA Cuts at: 1240 4509 1240 Size: 3269 3459 HaeII r_GCGC'y Cuts at: 941 1674 2378 3018 4162 4410 5640 6411 941 Size: 733 704 640 1144 248 1230 771 1258 Fragments arranged by size: 1258 1230 1144 771 733 704 640 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 673 705 1495 1527 1829 1861 2129 2156 2161 Size: 32 790 32 302 32 268 27 5 Cuts at: 2161 2188 2644 2676 2816 2848 3378 3410 4570 Size: 27 456 32 140 32 530 32 1160 Cuts at: 4570 4600 4602 4632 673 Size: 30 2 30 2769 Fragments arranged by size: 2769 1160 790 530 456 302 268 140 32 32 32 32 32 32 30 30 27 27 5 2 HincII GTy'rAC Cuts at: 1962 3767 1962 Size: 1805 4923 HindIII A'AGCT_T Cuts at: 3182 3182 Size: 6728 KasI G'GCGC_C Cuts at: 937 4158 4406 937 Size: 3221 248 3259 Fragments arranged by size: 3259 3221 248 KpnI G_GTAC'C Cuts at: 2861 3748 2861 Size: 887 5841 MluI A'CGCG_T Cuts at: 6161 6161 Size: 6728 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 103 5487 5671 103 Size: 5384 184 1160 Fragments arranged by size: 5384 1160 184 MscI TGG'CCA Cuts at: 4489 4489 Size: 6728 MslI CAynn'nnrTG Cuts at: 4717 4844 4717 Size: 127 6601 MspA1I CmG'CkG Cuts at: 222 504 878 1067 1170 1611 2143 2340 2539 Size: 282 374 189 103 441 532 197 199 Cuts at: 2539 3320 3453 3839 4153 4513 5297 5542 222 Size: 781 133 386 314 360 784 245 1408 Fragments arranged by size: 1408 784 781 532 441 386 374 360 314 282 245 199 197 189 133 103 NaeI GCC'GGC Cuts at: 151 4909 151 Size: 4758 1970 NarI GG'CG_CC Cuts at: 938 4159 4407 938 Size: 3221 248 3259 Fragments arranged by size: 3259 3221 248 NcoI C'CATG_G Cuts at: 1865 2967 1865 Size: 1102 5626 NdeI CA'TA_TG Cuts at: 207 207 Size: 6728 NgoMIV G'CCGG_C Cuts at: 149 4907 149 Size: 4758 1970 NsiI A_TGCA'T Cuts at: 429 429 Size: 6728 NspI r_CATG'y Cuts at: 427 1163 3781 4281 5884 427 Size: 736 2618 500 1603 1271 Fragments arranged by size: 2618 1603 1271 736 500 PasI CC'CwG_GG Cuts at: 2526 2526 Size: 6728 PciI A'CATG_T Cuts at: 1159 5880 1159 Size: 4721 2007 PflMI CCAn_nnn'nTGG Cuts at: 2869 2973 2869 Size: 104 6624 PfoI T'CCnGG_A Cuts at: 2948 3022 3248 2948 Size: 74 226 6428 Fragments arranged by size: 6428 226 74 PmeI GTTT'AAAC Cuts at: 3727 3727 Size: 6728 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3253 3285 5174 5206 3253 Size: 32 1889 32 4775 Fragments arranged by size: 4775 1889 32 32 PpuMI rG'GwC_Cy Cuts at: 585 2157 2946 3071 585 Size: 1572 789 125 4242 Fragments arranged by size: 4242 1572 789 125 PspOMI G'GGCC_C Cuts at: 716 968 2315 2393 2521 3691 716 Size: 252 1347 78 128 1170 3753 Fragments arranged by size: 3753 1347 1170 252 128 78 PstI C_TGCA'G Cuts at: 651 890 1691 1925 2774 3775 4460 651 Size: 239 801 234 849 1001 685 2919 Fragments arranged by size: 2919 1001 849 801 685 239 234 PvuI CG_AT'CG Cuts at: 104 104 Size: 6728 PvuII CAG'CTG Cuts at: 2143 2340 2539 4153 4513 2143 Size: 197 199 1614 360 4358 Fragments arranged by size: 4358 1614 360 199 197 RsrII CG'GwC_CG Cuts at: 4923 4923 Size: 6728 SacI G_AGCT'C Cuts at: 2753 3742 2753 Size: 989 5739 SacII CC_GC'GG Cuts at: 3321 3321 Size: 6728 SalI G'TCGA_C Cuts at: 3765 3765 Size: 6728 SapI GCTCTTCn'nnn_ Cuts at: 1186 1388 3005 3511 4751 4961 1186 Size: 202 1617 506 1240 210 2953 Fragments arranged by size: 2953 1617 1240 506 210 202 SbfI CC_TGCA'GG Cuts at: 3775 3775 Size: 6728 ScaI AGT'ACT Cuts at: 6245 6245 Size: 6728 SexAI A'CCwGG_T Cuts at: 1272 2366 3719 1272 Size: 1094 1353 4281 Fragments arranged by size: 4281 1353 1094 SfcI C'TryA_G Cuts at: 33 168 647 886 1569 1687 1921 2770 2926 Size: 135 479 239 683 118 234 849 156 Cuts at: 2926 3048 3771 4456 5424 5615 33 Size: 122 723 685 968 191 1146 Fragments arranged by size: 1146 968 849 723 685 683 479 239 234 191 156 135 122 118 SfoI GGC'GCC Cuts at: 939 4160 4408 939 Size: 3221 248 3259 Fragments arranged by size: 3259 3221 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 6728 SmaI CCC'GGG Cuts at: 193 3409 3750 193 Size: 3216 341 3171 Fragments arranged by size: 3216 3171 341 SmlI C'TyrA_G Cuts at: 175 679 1406 1429 1965 3236 3601 3883 4798 Size: 504 727 23 536 1271 365 282 915 Cuts at: 4798 5512 5774 175 Size: 714 262 1129 Fragments arranged by size: 1271 1129 915 727 714 536 504 365 282 262 23 SpeI A'CTAG_T Cuts at: 67 67 Size: 6728 SphI G_CATG'C Cuts at: 427 3781 4281 427 Size: 3354 500 2874 Fragments arranged by size: 3354 2874 500 StuI AGG'CCT Cuts at: 2554 2554 Size: 6728 StyI C'CwwG_G Cuts at: 392 1865 2057 2967 3034 3335 3553 3572 3862 Size: 1473 192 910 67 301 218 19 290 Cuts at: 3862 4216 392 Size: 354 2904 Fragments arranged by size: 2904 1473 910 354 301 290 218 192 67 19 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 201 2262 3565 4311 4444 4903 5112 201 Size: 2061 1303 746 133 459 209 1817 Fragments arranged by size: 2061 1817 1303 746 459 209 133 TatI w'GTAC_w Cuts at: 822 6243 822 Size: 5421 1307 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3036 3068 3331 3363 6245 6277 3036 Size: 32 263 32 2882 32 3487 Fragments arranged by size: 3487 2882 263 32 32 32 Tth111I GACn'n_nGTC Cuts at: 2944 3562 4525 2944 Size: 618 963 5147 Fragments arranged by size: 5147 963 618 XbaI T'CTAG_A Cuts at: 3759 3759 Size: 6728 XcmI CCAnnnn_n'nnnnTGG Cuts at: 465 835 4223 465 Size: 370 3388 2970 Fragments arranged by size: 3388 2970 370 XhoI C'TCGA_G Cuts at: 3236 3236 Size: 6728 XmaI C'CCGG_G Cuts at: 191 3407 3748 191 Size: 3216 341 3171 Fragments arranged by size: 3216 3171 341 XmnI GAAnn'nnTTC Cuts at: 3505 3505 Size: 6728 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI Enzymes that do not cut: AatII AfeI AflII AgeI AhdI AleI AloI AscI AvrII BaeI BbvCI BcgI BmtI BsiWI BsmBI BspEI BssHII BstEII BstZ17I FseI FspAI HpaI MfeI NheI NotI NruI PacI PmlI PshAI PsiI PspXI PsrI SanDI SfiI SgrAI SnaBI SrfI SspI SwaI ZraI