################################## # NO CUT ENZYMES ( 91 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI Asp718I AspEI AspHI AspI AvrII BamHI BbeI BbrPI BcgI BfrI BglI BlnI BsaAI BsaBI BsaWI BseAI BsiHKAI BsiWI BsmBI BspDI BspEI BspHI BsrDI BssSI Bst1107I BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DrdI Eam1105I Eco47III Eco81I EcoO65I EcoRV EcoT14I FseI HpaI KasI KpnI MamI MluI MroI NarI NcoI NdeI NheI NotI NruI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacI SalI SapI SbfI SexAI SfiI SfoI SgrAI SnaBI Sse8387I StyI SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AcyI 285 GACGCC G[GA]^CG[CT]C AviII 2862 TGCGCA TGC^GCA BalI 1091 TGGCCA TGG^CCA BanI 539 GGTGCC G^G[CT][GA]CC BbvCI 163 GCTGAGG CCTCAGC(-5/-2) BciVI 1802 GGATAC GTATCC(6/5) BsaHI 285 GACGCC G[GA]^CG[CT]C BsiEI 176 CGGCCG CG[GA][CT]^CG Bsp1407I 831 TGTACA T^GTACA BsrGI 831 TGTACA T^GTACA BssHII 56 GCGCGC G^CGCGC BstBI 2591 TTCGAA TT^CGAA DraIII 1334 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 176 CGGCCG C^GGCCG EarI 3283 GAAGAG CTCTTC(1/4) EclXI 176 CGGCCG C^GGCCG Eco52I 176 CGGCCG C^GGCCG EcoNI 2155 CCTTCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 2941 GAATTC G^AATTC EcoT22I 2486 ATGCAT ATGCA^T FspI 2862 TGCGCA TGC^GCA Hin1I 285 GACGCC G[GA]^CG[CT]C HincII 809 GTCAAC GT[CT]^[GA]AC HindII 809 GTCAAC GT[CT]^[GA]AC Ksp632I 3283 GAAGAG CTCTTC(1/4) MluNI 1091 TGGCCA TGG^CCA MscI 1091 TGGCCA TGG^CCA NsiI 2486 ATGCAT ATGCA^T NspV 2591 TTCGAA TT^CGAA PstI 2525 CTGCAG CTGCA^G PvuII 2256 CAGCTG CAG^CTG SfuI 2591 TTCGAA TT^CGAA SmaI 495 CCCGGG CCC^GGG SpeI 652 ACTAGT A^CTAGT SphI 1875 GCATGC GCATG^C SspBI 831 TGTACA T^GTACA SspI 1826 AATATT AAT^ATT StuI 1209 AGGCCT AGG^CCT Tsp45I 2120 GTCAC ^GT[GC]AC XbaI 2469 TCTAGA T^CTAGA XmaCI 495 CCCGGG C^CCGGG XmaI 495 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AcsI 745 AAATTT [GA]^AATT[CT] 2941 GAATTC ApaI 82 GGGCCC GGGCC^C 181 GGGCCC ApoI 745 AAATTT [GA]^AATT[CT] 2941 GAATTC AseI 1117 ATTAAT AT^TAAT 3160 ATTAAT AsnI 1117 ATTAAT AT^TAAT 3160 ATTAAT BglII 2691 AGATCT A^GATCT 3236 AGATCT BlpI 480 GCTCAGC GC^T[ACGT]AGC 2559 GCTCAGC Bpu1102I 480 GCTCAGC GC^T[ACGT]AGC 2559 GCTCAGC BsaI 2220 GAGACC GGTCTC(1/5) 3363 GAGACC BsgI 674 GTGCAG GTGCAG(16/14) 1030 GTGCAG BsmI 559 GAATGC GAATGC(1/-1) 1782 GAATGC BsrBI 314 CCGCTC CCGCTC(-3/-3) 33 GAGCGG BstAPI 387 GCAGAGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1716 GCAAACAATGC BstXI 519 CCAGCAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2054 CCATACAGTTGG BstYI 2691 AGATCT [GA]^GATC[CT] 3236 AGATCT CelII 480 GCTCAGC GC^T[ACGT]AGC 2559 GCTCAGC HaeII 160 GGCGCT [GA]GCGC^[CT] 473 AGCGCC HgaI 285 GACGC GACGC(5/10) 1317 GCGTC HindIII 941 AAGCTT A^AGCTT 3025 AAGCTT KspI 116 CCGCGG CCGC^GG 203 CCGCGG MaeII 1371 ACGT A^CGT 2901 ACGT MfeI 965 CAATTG C^AATTG 1256 CAATTG MflI 2691 AGATCT [GA]^GATC[CT] 3236 AGATCT MslI 114 CACCGCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2211 CACACCTGTG MunI 965 CAATTG C^AATTG 1256 CAATTG PflMI 1388 CCACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1444 CCAGCTCCTGG PshBI 1117 ATTAAT AT^TAAT 3160 ATTAAT SacII 116 CCGCGG CCGC^GG 203 CCGCGG ScaI 2230 AGTACT AGT^ACT 2239 AGTACT SmlI 991 CTTGAG C^T[CT][GA]AG 2076 CTCAAG Van91I 1388 CCACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1444 CCAGCTCCTGG XcmI 2653 CCAAATGAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2764 CCAAGTGGGGACTGG XhoII 2691 AGATCT [GA]^GATC[CT] 3236 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 92 enzymes) # ######################################### AccII AciI AfaI AflIII AluI AlwI AlwNI Asp700I AvaI AvaII BanII BbsI BbvI BclI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspLU11I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII FauI FbaI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NspI PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XmnI