Restriction map for
pF1KA1113
(Circular) (Six-base) MAPSORT of: pF1KA1113.seq Check: 9471 from: 1 to: 6494
pF1KA1113 6494 bp
With 182 enzymes: SgfI *
September 18, 2009 16:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 487 1320 487
Size: 833 5661
AccI GT'mk_AC
Cuts at: 3532 3532
Size: 6494
Acc65I G'GTAC_C
Cuts at: 3510 3510
Size: 6494
AclI AA'CG_TT
Cuts at: 6334 6334
Size: 6494
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 737 2471 3121 4319 4751 5498 5766 737
Size: 1734 650 1198 432 747 268 1465
Fragments arranged by size:
1734 1465 1198 747 650 432 268
AflIII A'CryG_T
Cuts at: 826 976 991 3846 4950 5927 826
Size: 150 15 2855 1104 977 1393
Fragments arranged by size:
2855 1393 1104 977 150 15
AgeI A'CCGG_T
Cuts at: 4882 4882
Size: 6494
AleI CACnn'nnGTG
Cuts at: 226 2323 226
Size: 2097 4397
AlwNI CAG_nnn'CTG
Cuts at: 901 1558 1732 3799 5366 901
Size: 657 174 2067 1567 2029
Fragments arranged by size:
2067 2029 1567 657 174
ApaI G_GGCC'C
Cuts at: 194 293 194
Size: 99 6395
ApaLI G'TGCA_C
Cuts at: 5264 5264
Size: 6494
ApoI r'AATT_y
Cuts at: 853 3049 3498 5847 6103 853
Size: 2196 449 2349 256 1244
Fragments arranged by size:
2349 2196 1244 449 256
AseI AT'TA_AT
Cuts at: 20 1226 3269 20
Size: 1206 2043 3245
Fragments arranged by size:
3245 2043 1206
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6494
AvaI C'yCGr_G
Cuts at: 321 571 603 3514 321
Size: 250 32 2911 3301
Fragments arranged by size:
3301 2911 250 32
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1015 1048 1015
Size: 33 6461
BamHI G'GATC_C
Cuts at: 3519 3519
Size: 6494
BanI G'GyrC_C
Cuts at: 647 3510 3924 4172 4207 647
Size: 2863 414 248 35 2934
Fragments arranged by size:
2934 2863 414 248 35
BanII G_rGCy'C
Cuts at: 194 293 328 527 2041 2669 3508 4538 194
Size: 99 35 199 1514 628 839 1030 2150
Fragments arranged by size:
2150 1514 1030 839 628 199 99 35
BbeI G_GCGC'C
Cuts at: 3928 4176 3928
Size: 248 6246
BbsI GAAGACnn'nnnn_
Cuts at: 535 776 2401 2774 3004 3434 535
Size: 241 1625 373 230 430 3595
Fragments arranged by size:
3595 1625 430 373 241 230
BbvCI CC'TCA_GC
Cuts at: 272 272
Size: 6494
BciVI GTATCCnnnnn_n'
Cuts at: 1904 4392 5153 6494 1904
Size: 2488 761 1341 1904
Fragments arranged by size:
2488 1904 1341 761
BclI T'GATC_A
Cuts at: 872 1814 2418 4014 872
Size: 942 604 1596 3352
Fragments arranged by size:
3352 1596 942 604
BfrBI ATG'CAT
Cuts at: 2596 2596
Size: 6494
BglII A'GATC_T
Cuts at: 2799 3344 4009 6046 2799
Size: 545 665 2037 3247
Fragments arranged by size:
3247 2037 665 545
BlpI GC'TnA_GC
Cuts at: 589 2668 3606 589
Size: 2079 938 3477
Fragments arranged by size:
3477 2079 938
Bme1580I G_kGCm'C
Cuts at: 194 293 367 599 652 4119 4212 5268 194
Size: 99 74 232 53 3467 93 1056 1420
Fragments arranged by size:
3467 1420 1056 232 99 93 74 53
BmrI ACTGGGnnnn_n'
Cuts at: 646 3706 3787 4122 6266 646
Size: 3060 81 335 2144 874
Fragments arranged by size:
3060 2144 874 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1901 2818 3366 4861 1901
Size: 917 548 1495 3534
Fragments arranged by size:
3534 1495 917 548
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 386 389 392 1580 1639 2252 386
Size: 3 3 1188 59 613 4628
Fragments arranged by size:
4628 1188 613 59 3 3
Bpu10I CC'TnA_GC
Cuts at: 272 3296 272
Size: 3024 3470
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1120 2169 3670 4549 5041 5339 1120
Size: 1049 1501 879 492 298 2275
Fragments arranged by size:
2275 1501 1049 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2322 3465 6198 2322
Size: 1143 2733 2618
Fragments arranged by size:
2733 2618 1143
BsaAI yAC'GTr
Cuts at: 3847 3847
Size: 6494
BsaBI GATnn'nnATC
Cuts at: 6 4008 6045 6
Size: 4002 2037 455
Fragments arranged by size:
4002 2037 455
BsaHI Gr'CG_yC
Cuts at: 394 3925 4173 4875 6371 394
Size: 3531 248 702 1496 517
Fragments arranged by size:
3531 1496 702 517 248
BsaWI w'CCGG_w
Cuts at: 3683 4204 4882 5156 5303 5714 3683
Size: 521 678 274 147 411 4463
Fragments arranged by size:
4463 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 384 414 384
Size: 30 6464
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 253 256 259 262 309 1796 2526 2747 2810
Size: 3 3 3 47 1487 730 221 63
Cuts at: 2810 253
Size: 3937
Fragments arranged by size:
3937 1487 730 221 63 47 3 3
3
BseYI C'CCAG_C
Cuts at: 3919 4677 5254 5750 3919
Size: 758 577 496 4663
Fragments arranged by size:
4663 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 803 1159 803
Size: 356 6138
BsiEI CG_ry'CG
Cuts at: 104 287 4036 4082 5290 104
Size: 183 3749 46 1208 1308
Fragments arranged by size:
3749 1308 1208 183 46
BsiHKAI G_wGCw'C
Cuts at: 3508 4286 4476 5268 3508
Size: 778 190 792 4734
Fragments arranged by size:
4734 792 778 190
BsmI GAATG_Cn'
Cuts at: 673 1896 673
Size: 1223 5271
Bsp1286I G_dGCh'C
Cuts at: 194 293 328 367 527 599 652 2041 2669
Size: 99 35 39 160 72 53 1389 628
Cuts at: 2669 3508 4119 4212 4286 4476 4538 5268 194
Size: 839 611 93 74 190 62 730 1420
Fragments arranged by size:
1420 1389 839 730 628 611 190 160
99 93 74 72 62 53 39 35
BspEI T'CCGG_A
Cuts at: 5714 5714
Size: 6494
BspHI T'CATG_A
Cuts at: 5670 5670
Size: 6494
BspMI ACCTGCnnnn'nnnn_
Cuts at: 487 1320 2579 2640 3544 3758 4060 4441 487
Size: 833 1259 61 904 214 302 381 2540
Fragments arranged by size:
2540 1259 904 833 381 302 214 61
BsrBI CCG'CTC
Cuts at: 50 143 424 4786 4840 6324 6489 50
Size: 93 281 4362 54 1484 165 55
Fragments arranged by size:
4362 1484 281 165 93 55 54
BsrDI GCAATG_nn'
Cuts at: 4406 4406
Size: 6494
BsrFI r'CCGG_y
Cuts at: 168 265 396 509 4492 4673 4882 168
Size: 97 131 113 3983 181 209 1780
Fragments arranged by size:
3983 1780 209 181 131 113 97
BsrGI T'GTAC_A
Cuts at: 939 939
Size: 6494
BssHII G'CGCG_C
Cuts at: 164 164
Size: 6494
BssSI C'ACGA_G
Cuts at: 4765 5123 4765
Size: 358 6136
BstAPI GCAn_nnn'nTGC
Cuts at: 501 1830 3871 501
Size: 1329 2041 3124
Fragments arranged by size:
3124 2041 1329
BstBI TT'CG_AA
Cuts at: 2700 4855 2700
Size: 2155 4339
BstXI CCAn_nnnn'nTGG
Cuts at: 634 2169 634
Size: 1535 4959
BstYI r'GATC_y
Cuts at: 1 2799 3344 3519 3986 4009 4344 4590 5602
Size: 2798 545 175 467 23 335 246 1012
Cuts at: 5602 5688 5700 6046 1
Size: 86 12 346 449
Fragments arranged by size:
2798 1012 545 467 449 346 335 246
175 86 23 12
BtgI C'CryG_G
Cuts at: 107 224 311 2987 107
Size: 117 87 2676 3614
Fragments arranged by size:
3614 2676 117 87
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2126 4444 4625 4788 2126
Size: 2318 181 163 3832
Fragments arranged by size:
3832 2318 181 163
BtsI GCAGTG_nn'
Cuts at: 3737 3737
Size: 6494
DraI TTT'AAA
Cuts at: 3493 3493
Size: 6494
DraIII CAC_nnn'GTG
Cuts at: 1447 1447
Size: 6494
DrdI GACnn_nn'nnGTC
Cuts at: 4200 5058 4200
Size: 858 5636
EaeI y'GGCC_r
Cuts at: 284 308 1199 3182 4079 4253 4644 4671 6016
Size: 24 891 1983 897 174 391 27 1345
Cuts at: 6016 284
Size: 762
Fragments arranged by size:
1983 1345 897 891 762 391 174 27
24
EagI C'GGCC_G
Cuts at: 284 4079 284
Size: 3795 2699
EarI CTCTTCn'nnn_
Cuts at: 3386 4517 4727 3386
Size: 1131 210 5153
Fragments arranged by size:
5153 1131 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 223 535 4702 5012 5158 6304 127
Size: 96 312 4167 310 146 1146 317
Fragments arranged by size:
4167 1146 317 312 310 146 96
EcoICRI GAG'CTC
Cuts at: 3506 3506
Size: 6494
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 386 389 392 737 1580 1639 2252 2471 3121
Size: 3 3 345 843 59 613 219 650
Cuts at: 3121 4319 4751 5498 5766 386
Size: 1198 432 747 268 1114
Fragments arranged by size:
1198 1114 843 747 650 613 432 345
268 219 59 3 3
EcoNI CCTnn'n_nnAGG
Cuts at: 2267 2267
Size: 6494
EcoO109I rG'GnC_Cy
Cuts at: 430 2074 2272 3359 3633 430
Size: 1644 198 1087 274 3291
Fragments arranged by size:
3291 1644 1087 274 198
EcoRI G'AATT_C
Cuts at: 3049 3498 3049
Size: 449 6045
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4513 4513
Size: 6494
FspI TGC'GCA
Cuts at: 2972 4275 2972
Size: 1303 5191
HaeII r_GCGC'y
Cuts at: 272 585 3928 4176 5198 6177 272
Size: 313 3343 248 1022 979 589
Fragments arranged by size:
3343 1022 979 589 313 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 384 416 727 759 2167 2199 2541 2573 4336
Size: 32 311 32 1408 32 342 32 1763
Cuts at: 4336 4366 4368 4398 384
Size: 30 2 30 2480
Fragments arranged by size:
2480 1763 1408 342 311 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 919 3533 919
Size: 2614 3880
HindIII A'AGCT_T
Cuts at: 1049 3133 1049
Size: 2084 4410
KasI G'GCGC_C
Cuts at: 3924 4172 3924
Size: 248 6246
KpnI G_GTAC'C
Cuts at: 3514 3514
Size: 6494
MfeI C'AATT_G
Cuts at: 1073 1364 1073
Size: 291 6203
MluI A'CGCG_T
Cuts at: 5927 5927
Size: 6494
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 885 2150 2878 5165 5349 885
Size: 1265 728 2287 184 2030
Fragments arranged by size:
2287 2030 1265 728 184
MscI TGG'CCA
Cuts at: 1201 4255 1201
Size: 3054 3440
MslI CAynn'nnrTG
Cuts at: 226 2323 4483 4610 226
Size: 2097 2160 127 2110
Fragments arranged by size:
2160 2110 2097 127
MspA1I CmG'CkG
Cuts at: 158 226 313 1925 2366 3605 3919 4279 5292
Size: 68 87 1612 441 1239 314 360 1013
Cuts at: 5292 5537 158
Size: 245 1115
Fragments arranged by size:
1612 1239 1115 1013 441 360 314 245
87 68
NaeI GCC'GGC
Cuts at: 267 398 511 4675 267
Size: 131 113 4164 2086
Fragments arranged by size:
4164 2086 131 113
NarI GG'CG_CC
Cuts at: 3925 4173 3925
Size: 248 6246
NcoI C'CATG_G
Cuts at: 107 107
Size: 6494
NgoMIV G'CCGG_C
Cuts at: 265 396 509 4673 265
Size: 131 113 4164 2086
Fragments arranged by size:
4164 2086 131 113
NsiI A_TGCA'T
Cuts at: 2598 2598
Size: 6494
NspI r_CATG'y
Cuts at: 830 980 995 1342 1665 1987 2526 3547 4047
Size: 150 15 347 323 322 539 1021 500
Cuts at: 4047 4954 830
Size: 907 2370
Fragments arranged by size:
2370 1021 907 539 500 347 323 322
150 15
PasI CC'CwG_GG
Cuts at: 1978 1978
Size: 6494
PciI A'CATG_T
Cuts at: 826 976 991 4950 826
Size: 150 15 3959 2370
Fragments arranged by size:
3959 2370 150 15
PflMI CCAn_nnn'nTGG
Cuts at: 1502 1558 1502
Size: 56 6438
PfoI T'CCnGG_A
Cuts at: 450 470 2289 3053 3167 450
Size: 20 1819 764 114 3777
Fragments arranged by size:
3777 1819 764 114 20
PmeI GTTT'AAAC
Cuts at: 3493 3493
Size: 6494
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5633 5665 5633
Size: 32 6462
PpuMI rG'GwC_Cy
Cuts at: 430 2074 2272 3359 430
Size: 1644 198 1087 3565
Fragments arranged by size:
3565 1644 1087 198
PsiI TTA'TAA
Cuts at: 3097 3097
Size: 6494
PspOMI G'GGCC_C
Cuts at: 190 289 190
Size: 99 6395
PstI C_TGCA'G
Cuts at: 2637 3541 4226 2637
Size: 904 685 4905
Fragments arranged by size:
4905 904 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6494
PvuII CAG'CTG
Cuts at: 2366 3919 4279 2366
Size: 1553 360 4581
Fragments arranged by size:
4581 1553 360
RsrII CG'GwC_CG
Cuts at: 4689 4689
Size: 6494
SacI G_AGCT'C
Cuts at: 3508 3508
Size: 6494
SacII CC_GC'GG
Cuts at: 227 314 227
Size: 87 6407
SalI G'TCGA_C
Cuts at: 3531 3531
Size: 6494
SanDI GG'GwC_CC
Cuts at: 430 430
Size: 6494
SapI GCTCTTCn'nnn_
Cuts at: 4517 4727 4517
Size: 210 6284
SbfI CC_TGCA'GG
Cuts at: 3541 3541
Size: 6494
ScaI AGT'ACT
Cuts at: 2340 2349 6011 2340
Size: 9 3662 2823
Fragments arranged by size:
3662 2823 9
SfcI C'TryA_G
Cuts at: 33 1294 2045 2633 3537 4222 5215 5406 33
Size: 1261 751 588 904 685 993 191 1121
Fragments arranged by size:
1261 1121 993 904 751 685 588 191
SfoI GGC'GCC
Cuts at: 3926 4174 3926
Size: 248 6246
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6494
SmaI CCC'GGG
Cuts at: 605 3516 605
Size: 2911 3583
SmlI C'TyrA_G
Cuts at: 1099 2184 3649 4564 5056 5318 1099
Size: 1085 1465 915 492 262 2275
Fragments arranged by size:
2275 1465 1085 915 492 262
SpeI A'CTAG_T
Cuts at: 67 760 67
Size: 693 5801
SphI G_CATG'C
Cuts at: 1987 3547 4047 1987
Size: 1560 500 4434
Fragments arranged by size:
4434 1560 500
SspI AAT'ATT
Cuts at: 1936 1936
Size: 6494
StuI AGG'CCT
Cuts at: 1319 1319
Size: 6494
StyI C'CwwG_G
Cuts at: 107 3628 3982 107
Size: 3521 354 2619
Fragments arranged by size:
3521 2619 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2175 4077 4210 4669 4878 2175
Size: 1902 133 459 209 3791
Fragments arranged by size:
3791 1902 459 209 133
TatI w'GTAC_w
Cuts at: 757 939 2338 2347 3320 3477 6009 757
Size: 182 1399 9 973 157 2532 1242
Fragments arranged by size:
2532 1399 1242 973 182 157 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2244 2276 6011 6043 2244
Size: 32 3735 32 2695
Fragments arranged by size:
3735 2695 32 32
Tth111I GACn'n_nGTC
Cuts at: 4291 4291
Size: 6494
XbaI T'CTAG_A
Cuts at: 2577 3525 2577
Size: 948 5546
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2768 2879 3989 2768
Size: 111 1110 5273
Fragments arranged by size:
5273 1110 111
XmaI C'CCGG_G
Cuts at: 603 3514 603
Size: 2911 3583
XmnI GAAnn'nnTTC
Cuts at: 964 1052 2135 3011 964
Size: 88 1083 876 4447
Fragments arranged by size:
4447 1083 876 88
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PsiI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BcgI BglI BmgBI BmtI BsiWI BsmBI BstEII BstZ17I
Bsu36I ClaI CspCI EcoRV FseI FspAI HpaI NdeI
NheI NotI NruI PacI PmlI PshAI PspXI PsrI
SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI