(Circular) (Six-base) MAPSORT of: pF1KA0847.seq Check: 1676 from: 1 to: 6845 pF1KA0847 6845 bp With 182 enzymes: SgfI * June 20, 2008 10:20 .. AatII G_ACGT'C Cuts at: 638 638 Size: 6845 AccI GT'mk_AC Cuts at: 474 3883 474 Size: 3409 3436 Acc65I G'GTAC_C Cuts at: 3861 3861 Size: 6845 AclI AA'CG_TT Cuts at: 6685 6685 Size: 6845 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 274 1334 1774 1877 1979 2132 2176 2287 2459 Size: 1060 440 103 102 153 44 111 172 Cuts at: 2459 2645 3236 4670 5102 5455 6117 274 Size: 186 591 1434 432 353 662 1002 Fragments arranged by size: 1434 1060 1002 662 591 440 432 353 186 172 153 111 103 102 44 AflIII A'CryG_T Cuts at: 571 749 2327 2691 4197 5997 6278 571 Size: 178 1578 364 1506 1800 281 1138 Fragments arranged by size: 1800 1578 1506 1138 364 281 178 AhdI GACnn_n'nnGTC Cuts at: 735 735 Size: 6845 AlwNI CAG_nnn'CTG Cuts at: 1495 2995 4150 5588 1495 Size: 1500 1155 1438 2752 Fragments arranged by size: 2752 1500 1438 1155 ApaLI G'TGCA_C Cuts at: 5683 5683 Size: 6845 ApoI r'AATT_y Cuts at: 209 868 1295 2413 2666 3263 3446 3849 6198 Size: 659 427 1118 253 597 183 403 2349 Cuts at: 6198 6454 209 Size: 256 600 Fragments arranged by size: 2349 1118 659 600 597 427 403 256 253 183 AseI AT'TA_AT Cuts at: 20 20 Size: 6845 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 6845 AvaI C'yCGr_G Cuts at: 434 647 3865 434 Size: 213 3218 3414 Fragments arranged by size: 3414 3218 213 AvrII C'CTAG_G Cuts at: 3399 3399 Size: 6845 BamHI G'GATC_C Cuts at: 3760 3870 3760 Size: 110 6735 BanI G'GyrC_C Cuts at: 2607 2884 3861 4275 4523 4558 2607 Size: 277 977 414 248 35 4894 Fragments arranged by size: 4894 977 414 277 248 35 BanII G_rGCy'C Cuts at: 1732 2207 3859 4889 1732 Size: 475 1652 1030 3688 Fragments arranged by size: 3688 1652 1030 475 BbeI G_GCGC'C Cuts at: 4279 4527 4279 Size: 248 6597 BbsI GAAGACnn'nnnn_ Cuts at: 726 1009 1339 2774 726 Size: 283 330 1435 4797 Fragments arranged by size: 4797 1435 330 283 BciVI GTATCCnnnnn_n' Cuts at: 4743 5799 6845 4743 Size: 1056 1046 4743 Fragments arranged by size: 4743 1056 1046 BclI T'GATC_A Cuts at: 1926 2388 4365 1926 Size: 462 1977 4406 Fragments arranged by size: 4406 1977 462 BfrBI ATG'CAT Cuts at: 685 685 Size: 6845 BglII A'GATC_T Cuts at: 414 2187 2510 4360 6397 414 Size: 1773 323 1850 2037 862 Fragments arranged by size: 2037 1850 1773 862 323 BlpI GC'TnA_GC Cuts at: 1162 3957 1162 Size: 2795 4050 Bme1580I G_kGCm'C Cuts at: 2889 4470 4563 5687 2889 Size: 1581 93 1124 4047 Fragments arranged by size: 4047 1581 1124 93 BmgBI CAC'GTC Cuts at: 3596 3596 Size: 6845 BmrI ACTGGGnnnn_n' Cuts at: 1005 3240 3554 4057 4138 4473 6617 1005 Size: 2235 314 503 81 335 2144 1233 Fragments arranged by size: 2235 2144 1233 503 335 314 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2532 2982 3500 5212 2532 Size: 450 518 1712 4165 Fragments arranged by size: 4165 1712 518 450 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 804 848 1133 1498 1540 1885 1894 2008 2051 Size: 44 285 365 42 345 9 114 43 Cuts at: 2051 2972 3386 3690 804 Size: 921 414 304 3959 Fragments arranged by size: 3959 921 414 365 345 304 285 114 44 43 42 9 Bpu10I CC'TnA_GC Cuts at: 1679 1726 1679 Size: 47 6798 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1528 1871 2345 3078 4021 4900 5614 5912 1528 Size: 343 474 733 943 879 714 298 2461 Fragments arranged by size: 2461 943 879 733 714 474 343 298 BsaI GGTCTCn'nnnn_ Cuts at: 1504 2783 6549 1504 Size: 1279 3766 1800 Fragments arranged by size: 3766 1800 1279 BsaAI yAC'GTr Cuts at: 4198 4198 Size: 6845 BsaBI GATnn'nnATC Cuts at: 6 4359 6396 6 Size: 4353 2037 455 Fragments arranged by size: 4353 2037 455 BsaHI Gr'CG_yC Cuts at: 635 2059 4276 4524 5226 6722 635 Size: 1424 2217 248 702 1496 758 Fragments arranged by size: 2217 1496 1424 758 702 248 BsaWI w'CCGG_w Cuts at: 4034 4555 5233 5644 5791 6065 4034 Size: 521 678 411 147 274 4814 Fragments arranged by size: 4814 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 2519 2549 3415 3445 3546 3576 2519 Size: 30 866 30 101 30 5788 Fragments arranged by size: 5788 866 101 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 134 1333 1864 2505 3001 3133 3169 3497 3560 Size: 1199 531 641 496 132 36 328 63 Cuts at: 3560 3657 3693 134 Size: 97 36 3286 Fragments arranged by size: 3286 1199 641 531 496 328 132 97 63 36 36 BseYI C'CCAG_C Cuts at: 468 3682 3739 4270 5028 5693 6101 468 Size: 3214 57 531 758 665 408 1212 Fragments arranged by size: 3214 1212 758 665 531 408 57 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1565 1565 Size: 6845 BsiEI CG_ry'CG Cuts at: 104 4387 4433 5663 104 Size: 4283 46 1230 1286 Fragments arranged by size: 4283 1286 1230 46 BsiHKAI G_wGCw'C Cuts at: 1732 3167 3481 3859 4637 4827 5687 1732 Size: 1435 314 378 778 190 860 2890 Fragments arranged by size: 2890 1435 860 778 378 314 190 BsmI GAATG_Cn' Cuts at: 500 500 Size: 6845 BsmBI CGTCTCn'nnnn_ Cuts at: 3323 3323 Size: 6845 Bsp1286I G_dGCh'C Cuts at: 1732 2207 2889 3167 3481 3859 4470 4563 4637 Size: 475 682 278 314 378 611 93 74 Cuts at: 4637 4827 4889 5687 1732 Size: 190 62 798 2890 Fragments arranged by size: 2890 798 682 611 475 378 314 278 190 93 74 62 BspHI T'CATG_A Cuts at: 2322 2759 5277 2322 Size: 437 2518 3890 Fragments arranged by size: 3890 2518 437 BspMI ACCTGCnnnn'nnnn_ Cuts at: 649 1522 3895 4109 4411 4792 649 Size: 873 2373 214 302 381 2702 Fragments arranged by size: 2702 2373 873 381 302 214 BsrBI CCG'CTC Cuts at: 50 1388 5137 5191 6675 6840 50 Size: 1338 3749 54 1484 165 55 Fragments arranged by size: 3749 1484 1338 165 55 54 BsrDI GCAATG_nn' Cuts at: 1710 3729 4757 1710 Size: 2019 1028 3798 Fragments arranged by size: 3798 2019 1028 BsrFI r'CCGG_y Cuts at: 4843 5024 4843 Size: 181 6664 BssSI C'ACGA_G Cuts at: 3357 5116 5824 3357 Size: 1759 708 4378 Fragments arranged by size: 4378 1759 708 BstAPI GCAn_nnn'nTGC Cuts at: 1969 4222 1969 Size: 2253 4592 BstBI TT'CG_AA Cuts at: 5206 5206 Size: 6845 BstEII G'GTnAC_C Cuts at: 1370 1370 Size: 6845 BstXI CCAn_nnnn'nTGG Cuts at: 1342 3281 1342 Size: 1939 4906 BstYI r'GATC_y Cuts at: 1 414 1216 2187 2510 3460 3502 3694 3760 Size: 413 802 971 323 950 42 192 66 Cuts at: 3760 3870 4337 4360 4695 4941 5247 5259 5345 Size: 110 467 23 335 246 306 12 86 Cuts at: 5345 6397 1 Size: 1052 449 Fragments arranged by size: 1052 971 950 802 467 449 413 335 323 306 246 192 110 86 66 42 23 12 Bsu36I CC'TnA_GG Cuts at: 1947 1947 Size: 6845 BtgI C'CryG_G Cuts at: 1329 2198 1329 Size: 869 5976 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1181 4795 4976 5139 1181 Size: 3614 181 163 2887 Fragments arranged by size: 3614 2887 181 163 BtsI GCAGTG_nn' Cuts at: 4088 4088 Size: 6845 ClaI AT'CG_AT Cuts at: 369 369 Size: 6845 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 609 644 609 Size: 35 6810 DraI TTT'AAA Cuts at: 1934 3844 1934 Size: 1910 4935 DrdI GACnn_nn'nnGTC Cuts at: 1232 4551 5895 1232 Size: 3319 1344 2182 Fragments arranged by size: 3319 2182 1344 EaeI y'GGCC_r Cuts at: 1332 3334 4430 4604 4995 5022 6367 1332 Size: 2002 1096 174 391 27 1345 1810 Fragments arranged by size: 2002 1810 1345 1096 391 174 27 EagI C'GGCC_G Cuts at: 4430 4430 Size: 6845 EarI CTCTTCn'nnn_ Cuts at: 900 1253 1310 2435 3805 4868 5078 900 Size: 353 57 1125 1370 1063 210 2667 Fragments arranged by size: 2667 1370 1125 1063 353 210 57 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 5053 5795 5941 6655 5053 Size: 742 146 714 5243 Fragments arranged by size: 5243 742 714 146 EcoICRI GAG'CTC Cuts at: 1730 3857 1730 Size: 2127 4718 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 274 804 848 1133 1334 1498 1540 1774 1877 Size: 530 44 285 201 164 42 234 103 Cuts at: 1877 1885 1894 1979 2008 2051 2132 2176 2287 Size: 8 9 85 29 43 81 44 111 Cuts at: 2287 2459 2645 2972 3236 3386 3690 4670 5102 Size: 172 186 327 264 150 304 980 432 Cuts at: 5102 5455 6117 274 Size: 353 662 1002 Fragments arranged by size: 1002 980 662 530 432 353 327 304 285 264 234 201 186 172 164 150 111 103 85 81 44 44 43 42 29 9 8 EcoNI CCTnn'n_nnAGG Cuts at: 2276 3705 3708 2276 Size: 1429 3 5413 Fragments arranged by size: 5413 1429 3 EcoO109I rG'GnC_Cy Cuts at: 1785 1909 1978 2092 3713 3984 1785 Size: 124 69 114 1621 271 4646 Fragments arranged by size: 4646 1621 271 124 114 69 EcoRI G'AATT_C Cuts at: 868 3849 868 Size: 2981 3864 EcoRV GAT'ATC Cuts at: 135 2741 135 Size: 2606 4239 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1657 2113 4864 1657 Size: 456 2751 3638 Fragments arranged by size: 3638 2751 456 FspI TGC'GCA Cuts at: 4626 4626 Size: 6845 HaeII r_GCGC'y Cuts at: 4279 4527 5757 6528 4279 Size: 248 1230 771 4596 Fragments arranged by size: 4596 1230 771 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 351 383 721 753 877 909 2502 2534 2816 Size: 32 338 32 124 32 1593 32 282 Cuts at: 2816 2848 3380 3412 3415 3447 4687 4717 4719 Size: 32 532 32 3 32 1240 30 2 Cuts at: 4719 4749 351 Size: 30 2447 Fragments arranged by size: 2447 1593 1240 532 338 282 124 32 32 32 32 32 32 32 30 30 3 2 HincII GTy'rAC Cuts at: 1722 2144 3288 3884 1722 Size: 422 1144 596 4683 Fragments arranged by size: 4683 1144 596 422 HindIII A'AGCT_T Cuts at: 1288 2154 2641 3046 1288 Size: 866 487 405 5087 Fragments arranged by size: 5087 866 487 405 HpaI GTT'AAC Cuts at: 1722 1722 Size: 6845 KasI G'GCGC_C Cuts at: 4275 4523 4275 Size: 248 6597 KpnI G_GTAC'C Cuts at: 3865 3865 Size: 6845 MfeI C'AATT_G Cuts at: 1082 1082 Size: 6845 MluI A'CGCG_T Cuts at: 6278 6278 Size: 6845 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 126 382 2634 3440 5604 5788 126 Size: 256 2252 806 2164 184 1183 Fragments arranged by size: 2252 2164 1183 806 256 184 MscI TGG'CCA Cuts at: 1334 4606 1334 Size: 3272 3573 MslI CAynn'nnrTG Cuts at: 754 1628 4834 4961 754 Size: 874 3206 127 2638 Fragments arranged by size: 3206 2638 874 127 MspA1I CmG'CkG Cuts at: 777 3214 3956 4270 4630 5414 5659 777 Size: 2437 742 314 360 784 245 1963 Fragments arranged by size: 2437 1963 784 742 360 314 245 NaeI GCC'GGC Cuts at: 5026 5026 Size: 6845 NarI GG'CG_CC Cuts at: 4276 4524 4276 Size: 248 6597 NcoI C'CATG_G Cuts at: 1329 2198 1329 Size: 869 5976 NgoMIV G'CCGG_C Cuts at: 5024 5024 Size: 6845 NsiI A_TGCA'T Cuts at: 687 687 Size: 6845 NspI r_CATG'y Cuts at: 231 575 591 753 2331 2695 3898 4398 6001 Size: 344 16 162 1578 364 1203 500 1603 Cuts at: 6001 231 Size: 1075 Fragments arranged by size: 1603 1578 1203 1075 500 364 344 162 16 PasI CC'CwG_GG Cuts at: 2428 2428 Size: 6845 PciI A'CATG_T Cuts at: 571 749 2327 2691 5997 571 Size: 178 1578 364 3306 1419 Fragments arranged by size: 3306 1578 1419 364 178 PflMI CCAn_nnn'nTGG Cuts at: 1366 1760 1366 Size: 394 6451 PfoI T'CCnGG_A Cuts at: 1871 3498 3706 1871 Size: 1627 208 5010 Fragments arranged by size: 5010 1627 208 PmeI GTTT'AAAC Cuts at: 3844 3844 Size: 6845 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 659 691 5291 5323 659 Size: 32 4600 32 2181 Fragments arranged by size: 4600 2181 32 32 PpuMI rG'GwC_Cy Cuts at: 1785 1909 1978 2092 3713 1785 Size: 124 69 114 1621 4917 Fragments arranged by size: 4917 1621 124 114 69 PspXI vC'TCGA_Gb Cuts at: 647 647 Size: 6845 PstI C_TGCA'G Cuts at: 1974 3892 4577 1974 Size: 1918 685 4242 Fragments arranged by size: 4242 1918 685 PvuI CG_AT'CG Cuts at: 104 104 Size: 6845 PvuII CAG'CTG Cuts at: 777 3214 4270 4630 777 Size: 2437 1056 360 2992 Fragments arranged by size: 2992 2437 1056 360 RsrII CG'GwC_CG Cuts at: 5040 5040 Size: 6845 SacI G_AGCT'C Cuts at: 1732 3859 1732 Size: 2127 4718 SalI G'TCGA_C Cuts at: 3882 3882 Size: 6845 SapI GCTCTTCn'nnn_ Cuts at: 4868 5078 4868 Size: 210 6635 SbfI CC_TGCA'GG Cuts at: 3892 3892 Size: 6845 ScaI AGT'ACT Cuts at: 1862 6362 1862 Size: 4500 2345 SfcI C'TryA_G Cuts at: 33 1814 1970 3888 4573 5541 5732 33 Size: 1781 156 1918 685 968 191 1146 Fragments arranged by size: 1918 1781 1146 968 685 191 156 SfoI GGC'GCC Cuts at: 4277 4525 4277 Size: 248 6597 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 6845 SmaI CCC'GGG Cuts at: 3867 3867 Size: 6845 SmlI C'TyrA_G Cuts at: 647 1507 1886 2360 3093 4000 4915 5629 5891 Size: 860 379 474 733 907 915 714 262 Cuts at: 5891 647 Size: 1601 Fragments arranged by size: 1601 915 907 860 733 714 474 379 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 6845 SphI G_CATG'C Cuts at: 3898 4398 3898 Size: 500 6345 StuI AGG'CCT Cuts at: 3363 3363 Size: 6845 StyI C'CwwG_G Cuts at: 221 1329 1365 1912 2198 2227 3081 3268 3399 Size: 1108 36 547 286 29 854 187 131 Cuts at: 3399 3804 3979 4333 221 Size: 405 175 354 2733 Fragments arranged by size: 2733 1108 854 547 405 354 286 187 175 131 36 29 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1752 3249 3369 3543 4428 4561 5020 5229 1752 Size: 1497 120 174 885 133 459 209 3368 Fragments arranged by size: 3368 1497 885 459 209 174 133 120 TatI w'GTAC_w Cuts at: 568 1860 6360 568 Size: 1292 4500 1053 Fragments arranged by size: 4500 1292 1053 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 140 172 3546 3578 6362 6394 140 Size: 32 3374 32 2784 32 591 Fragments arranged by size: 3374 2784 591 32 32 32 Tth111I GACn'n_nGTC Cuts at: 3040 4642 3040 Size: 1602 5243 XbaI T'CTAG_A Cuts at: 882 3250 3876 882 Size: 2368 626 3851 Fragments arranged by size: 3851 2368 626 XcmI CCAnnnn_n'nnnnTGG Cuts at: 465 2070 4340 465 Size: 1605 2270 2970 Fragments arranged by size: 2970 2270 1605 XhoI C'TCGA_G Cuts at: 647 647 Size: 6845 XmaI C'CCGG_G Cuts at: 3865 3865 Size: 6845 XmnI GAAnn'nnTTC Cuts at: 500 1559 1640 1839 1862 2855 500 Size: 1059 81 199 23 993 4490 Fragments arranged by size: 4490 1059 993 199 81 23 ZraI GAC'GTC Cuts at: 636 636 Size: 6845 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflIII AhdI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PspXI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AflII AgeI AleI AlfI AloI ApaI AscI BaeI BbvCI BcgI BglI BmtI BsiWI BspEI BsrGI BssHII BstZ17I DraIII FseI FspAI NdeI NheI NotI NruI PacI PmlI PshAI PsiI PspOMI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI