Restriction map for pF1KA0709
 (Linear) (Six-base) MAPSORT of: KIAA0709.seq  Check: 1206  from: 1  to: 4437



KIAA0709.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:35  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      50    3959    4437 
   Size:         50    3909     478

  Fragments arranged by size: 

                3909     478      50
 
AccI GT'mk_AC

Cuts at:      0     524    4437 
   Size:        524    3913
 
Acc65I G'GTAC_C

Cuts at:      0     265    4437 
   Size:        265    4172
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     123    2682    3403    4437 
   Size:        123    2559     721    1034

  Fragments arranged by size: 

                2559    1034     721     123
 
AfeI AGC'GCT

Cuts at:      0     226     670    3362    3880    4220    4437 
   Size:        226     444    2692     518     340     217

  Fragments arranged by size: 

                2692     518     444     340     226     217
 
AhdI GACnn_n'nnGTC

Cuts at:      0     387    4437 
   Size:        387    4050
 
AleI CACnn'nnGTG

Cuts at:      0    1951    4042    4437 
   Size:       1951    2091     395

  Fragments arranged by size: 

                2091    1951     395
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     824    1078    4437 
   Size:        824     254    3359

  Fragments arranged by size: 

                3359     824     254
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2195    2227    2697    2729    4437 
   Size:       2195      32     470      32    1708

  Fragments arranged by size: 

                2195    1708     470      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     279    1484    1941    2118    2133    3792    4437 
   Size:        279    1205     457     177      15    1659     645

  Fragments arranged by size: 

                1659    1205     645     457     279     177      15
 
ApaI G_GGCC'C

Cuts at:      0       8     408    1459    1920    2127    2646    3746    4067 
   Size:          8     400    1051     461     207     519    1100     321

Cuts at:   4067    4081    4437 
   Size:         14     356

  Fragments arranged by size: 

                1100    1051     519     461     400     356     321     207
                  14       8
 
ApaLI G'TGCA_C

Cuts at:      0     558    1644    2587    4437 
   Size:        558    1086     943    1850

  Fragments arranged by size: 

                1850    1086     943     558
 
ApoI r'AATT_y

Cuts at:      0    1279    1353    4437 
   Size:       1279      74    3084

  Fragments arranged by size: 

                3084    1279      74
 
AvaI C'yCGr_G

Cuts at:      0      64    1026    1831    2206    2639    3375    3527    4068 
   Size:         64     962     805     375     433     736     152     541

Cuts at:   4068    4437 
   Size:        369

  Fragments arranged by size: 

                 962     805     736     541     433     375     369     152
                  64
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     249     282    1827    1860    2429    2462    4437 
   Size:        249      33    1545      33     569      33    1975

  Fragments arranged by size: 

                1975    1545     569     249      33      33      33
 
BamHI G'GATC_C

Cuts at:      0    2253    2927    4437 
   Size:       2253     674    1510

  Fragments arranged by size: 

                2253    1510     674
 
BanI G'GyrC_C

Cuts at:      0      68      91     265     298     433    1397    1637    2739 
   Size:         68      23     174      33     135     964     240    1102

Cuts at:   2739    2822    3174    3403    3979    4136    4313    4345    4437 
   Size:         83     352     229     576     157     177      32      92

  Fragments arranged by size: 

                1102     964     576     352     240     229     177     174
                 157     135      92      83      68      33      32      23
            
 
BanII G_rGCy'C

Cuts at:      0       8     408    1106    1412    1459    1920    2127    2456 
   Size:          8     400     698     306      47     461     207     329

Cuts at:   2456    2646    3188    3349    3389    3577    3746    4067    4081 
   Size:        190     542     161      40     188     169     321      14

Cuts at:   4081    4437 
   Size:        356

  Fragments arranged by size: 

                 698     542     461     400     356     329     321     306
                 207     190     188     169     161      47      40      14
                   8
 
BbeI G_GCGC'C

Cuts at:      0      95    1641    3983    4140    4317    4437 
   Size:         95    1546    2342     157     177     120

  Fragments arranged by size: 

                2342    1546     177     157     120      95
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     120    1616    4437 
   Size:        120    1496    2821

  Fragments arranged by size: 

                2821    1496     120
 
BbvCI CC'TCA_GC

Cuts at:      0    3255    4437 
   Size:       3255    1182
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3863    3897    4437 
   Size:       3863      34     540

  Fragments arranged by size: 

                3863     540      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0      12      89    1155    1463    1847    1997    2104    2471 
   Size:         12      77    1066     308     384     150     107     367

Cuts at:   2471    3172    3326    3478    4437 
   Size:        701     154     152     959

  Fragments arranged by size: 

                1066     959     701     384     367     308     154     152
                 150     107      77      12
 
BglII A'GATC_T

Cuts at:      0    2186    4437 
   Size:       2186    2251
 
BlpI GC'TnA_GC

Cuts at:      0     820    1528    2134    4090    4193    4437 
   Size:        820     708     606    1956     103     244

  Fragments arranged by size: 

                1956     820     708     606     244     103
 
Bme1580I G_kGCm'C

Cuts at:      0       8     286     408     562    1459    1648    1920    1948 
   Size:          8     278     122     154     897     189     272      28

Cuts at:   1948    2127    2591    2646    2742    3177    3331    3382    3491 
   Size:        179     464      55      96     435     154      51     109

Cuts at:   3491    3746    4067    4081    4350    4437 
   Size:        255     321      14     269      87

  Fragments arranged by size: 

                 897     464     435     321     278     272     269     255
                 189     179     154     154     122     109      96      87
                  55      51      28      14       8
 
BmgBI CAC'GTC

Cuts at:      0    1577    2445    4437 
   Size:       1577     868    1992

  Fragments arranged by size: 

                1992    1577     868
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     385     878     972     983    1731    2817    3180    3203 
   Size:        385     493      94      11     748    1086     363      23

Cuts at:   3203    4068    4437 
   Size:        865     369

  Fragments arranged by size: 

                1086     865     748     493     385     369     363      94
                  23      11
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2544    2598    3591    4437 
   Size:       2544      54     993     846

  Fragments arranged by size: 

                2544     993     846      54
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     184    1097    1453    1934    2301    4353    4437 
   Size:        184     913     356     481     367    2052      84

  Fragments arranged by size: 

                2052     913     481     367     356     184      84
 
Bpu10I CC'TnA_GC

Cuts at:      0    2614    3255    3343    4437 
   Size:       2614     641      88    1094

  Fragments arranged by size: 

                2614    1094     641      88
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     460     934    2453    4437 
   Size:        460     474    1519    1984

  Fragments arranged by size: 

                1984    1519     474     460
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     243     700    3295    3600    4437 
   Size:        243     457    2595     305     837

  Fragments arranged by size: 

                2595     837     457     305     243
 
BsaAI yAC'GTr

Cuts at:      0    2534    2561    3486    3618    3666    4437 
   Size:       2534      27     925     132      48     771

  Fragments arranged by size: 

                2534     925     771     132      48      27
 
BsaHI Gr'CG_yC

Cuts at:      0      92     104    1638    1957    3980    4137    4314    4437 
   Size:         92      12    1534     319    2023     157     177     123

  Fragments arranged by size: 

                2023    1534     319     177     157     123      92      12
            
 
BsaWI w'CCGG_w

Cuts at:      0    2036    2318    4307    4437 
   Size:       2036     282    1989     130

  Fragments arranged by size: 

                2036    1989     282     130
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1303    1333    1717    1747    2356    2386    2491    2521 
   Size:       1303      30     384      30     609      30     105      30

Cuts at:   2521    2716    2746    3919    3949    4437 
   Size:        195      30    1173      30     488

  Fragments arranged by size: 

                1303    1173     609     488     384     195     105      30
                  30      30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      52     505     853    1009    1327    1561    2167    2170 
   Size:         52     453     348     156     318     234     606       3

Cuts at:   2170    3313    3583    3616    3740    4437 
   Size:       1143     270      33     124     697

  Fragments arranged by size: 

                1143     697     606     453     348     318     270     234
                 156     124      52      33       3
 
BseYI C'CCAG_C

Cuts at:      0     221     671    1602    1987    2015    2087    2118    2126 
   Size:        221     450     931     385      28      72      31       8

Cuts at:   2126    3026    3275    3354    3855    3987    4081    4233    4437 
   Size:        900     249      79     501     132      94     152     204

  Fragments arranged by size: 

                 931     900     501     450     385     249     221     204
                 152     132      94      79      72      31      28       8
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58     585    2541    2656    3229    3276    4437 
   Size:         58     527    1956     115     573      47    1161

  Fragments arranged by size: 

                1956    1161     573     527     115      58      47
 
BsiEI CG_ry'CG

Cuts at:      0      12      16      84    1100    2319    3285    4437 
   Size:         12       4      68    1016    1219     966    1152

  Fragments arranged by size: 

                1219    1152    1016     966      68      12       4
 
BsiHKAI G_wGCw'C

Cuts at:      0     507     562    1648    2162    2525    2591    3389    3827 
   Size:        507      55    1086     514     363      66     798     438

Cuts at:   3827    3950    4437 
   Size:        123     487

  Fragments arranged by size: 

                1086     798     514     507     487     438     363     123
                  66      55
 
BsmI GAATG_Cn'

Cuts at:      0    3061    4437 
   Size:       3061    1376
 
Bsp1286I G_dGCh'C

Cuts at:      0       8     286     408     507     562    1106    1412    1459 
   Size:          8     278     122      99      55     544     306      47

Cuts at:   1459    1648    1920    1948    2127    2162    2456    2525    2591 
   Size:        189     272      28     179      35     294      69      66

Cuts at:   2591    2646    2742    3177    3188    3331    3349    3382    3389 
   Size:         55      96     435      11     143      18      33       7

Cuts at:   3389    3491    3577    3746    3827    3950    4067    4081    4350 
   Size:        102      86     169      81     123     117      14     269

Cuts at:   4350    4437 
   Size:         87

  Fragments arranged by size: 

                 544     435     306     294     278     272     269     189
                 179     169     143     123     122     117     102      99
                  96      87      86      81      69      66      55      55
                  47      35      33      28      18      14      11       8
                   7
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      50     171    2897    3959    4437 
   Size:         50     121    2726    1062     478

  Fragments arranged by size: 

                2726    1062     478     121      50
 
BsrBI CCG'CTC

Cuts at:      0     917    2067    3546    3892    4437 
   Size:        917    1150    1479     346     545

  Fragments arranged by size: 

                1479    1150     917     545     346
 
BsrFI r'CCGG_y

Cuts at:      0      11      15     248    1768    1931    2324    4437 
   Size:         11       4     233    1520     163     393    2113

  Fragments arranged by size: 

                2113    1520     393     233     163      11       4
 
BsrGI T'GTAC_A

Cuts at:      0    1805    2783    4437 
   Size:       1805     978    1654

  Fragments arranged by size: 

                1805    1654     978
 
BssSI C'ACGA_G

Cuts at:      0     517     838     912    2215    4187    4437 
   Size:        517     321      74    1303    1972     250

  Fragments arranged by size: 

                1972    1303     517     321     250      74
 
BstEII G'GTnAC_C

Cuts at:      0     379     448     625    1668    3033    4437 
   Size:        379      69     177    1043    1365    1404

  Fragments arranged by size: 

                1404    1365    1043     379     177      69
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1121    3268    4437 
   Size:       1121    2147    1169

  Fragments arranged by size: 

                2147    1169    1121
 
BstYI r'GATC_y

Cuts at:      0     813     833    1977    2186    2210    2253    2417    2426 
   Size:        813      20    1144     209      24      43     164       9

Cuts at:   2426    2927    4437 
   Size:        501    1510

  Fragments arranged by size: 

                1510    1144     813     501     209     164      43      24
                  20       9
 
BtgI C'CryG_G

Cuts at:      0     144     308     546     594    1094    1554    1653    1842 
   Size:        144     164     238      48     500     460      99     189

Cuts at:   1842    1877    3306    3771    4163    4437 
   Size:         35    1429     465     392     274

  Fragments arranged by size: 

                1429     500     465     460     392     274     238     189
                 164     144      99      48      35
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1041    2702    4437 
   Size:       1041    1661    1735

  Fragments arranged by size: 

                1735    1661    1041
 
BtsI GCAGTG_nn'

Cuts at:      0     280     468     934    1173    2386    2401    3744    3825 
   Size:        280     188     466     239    1213      15    1343      81

Cuts at:   3825    4437 
   Size:        612

  Fragments arranged by size: 

                1343    1213     612     466     280     239     188      81
                  15
 
DraIII CAC_nnn'GTG

Cuts at:      0     379     887    2166    4437 
   Size:        379     508    1279    2271

  Fragments arranged by size: 

                2271    1279     508     379
 
EaeI y'GGCC_r

Cuts at:      0       9      13      81     292     465    1097    1862    2141 
   Size:          9       4      68     211     173     632     765     279

Cuts at:   2141    2168    2387    3270    4437 
   Size:         27     219     883    1167

  Fragments arranged by size: 

                1167     883     765     632     279     219     211     173
                  68      27       9       4
 
EagI C'GGCC_G

Cuts at:      0       9      13      81    1097    4437 
   Size:          9       4      68    1016    3340

  Fragments arranged by size: 

                3340    1016      68       9       4
 
EarI CTCTTCn'nnn_

Cuts at:      0    2077    4437 
   Size:       2077    2360
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1285    2614    4437 
   Size:       1285    1329    1823

  Fragments arranged by size: 

                1823    1329    1285
 
EcoICRI GAG'CTC

Cuts at:      0    3387    4437 
   Size:       3387    1050
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     123     184    1097    1453    1934    2301    2682    3403 
   Size:        123      61     913     356     481     367     381     721

Cuts at:   3403    4353    4437 
   Size:        950      84

  Fragments arranged by size: 

                 950     913     721     481     381     367     356     123
                  84      61
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    3123    3517    4437 
   Size:       3123     394     920

  Fragments arranged by size: 

                3123     920     394
 
EcoO109I rG'GnC_Cy

Cuts at:      0       4     404     495    1455    1755    2105    2123    3680 
   Size:          4     400      91     960     300     350      18    1557

Cuts at:   3680    3743    4063    4064    4078    4334    4437 
   Size:         63     320       1      14     256     103

  Fragments arranged by size: 

                1557     960     400     350     320     300     256     103
                  91      63      18      14       4       1
 
EcoRI G'AATT_C

Cuts at:      0    1279    4437 
   Size:       1279    3158
 
FseI GG_CCGG'CC

Cuts at:      0      15      19    4437 
   Size:         15       4    4418

  Fragments arranged by size: 

                4418      15       4
 
FspI TGC'GCA

Cuts at:      0    3538    4437 
   Size:       3538     899
 
HaeII r_GCGC'y

Cuts at:      0      95     228     672    1641    1861    1877    2280    2697 
   Size:         95     133     444     969     220      16     403     417

Cuts at:   2697    3364    3882    3983    4140    4148    4222    4317    4437 
   Size:        667     518     101     157       8      74      95     120

  Fragments arranged by size: 

                 969     667     518     444     417     403     220     157
                 133     120     101      95      95      74      16       8
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     373     405    1642    1674    2356    2388    4437 
   Size:        373      32    1237      32     682      32    2049

  Fragments arranged by size: 

                2049    1237     682     373      32      32      32
 
HindIII A'AGCT_T

Cuts at:      0    4180    4437 
   Size:       4180     257
 
KasI G'GCGC_C

Cuts at:      0      91    1637    3979    4136    4313    4437 
   Size:         91    1546    2342     157     177     124

  Fragments arranged by size: 

                2342    1546     177     157     124      91
 
KpnI G_GTAC'C

Cuts at:      0     269    4437 
   Size:        269    4168
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     914    1552    4079    4437 
   Size:        914     638    2527     358

  Fragments arranged by size: 

                2527     914     638     358
 
MscI TGG'CCA

Cuts at:      0     294     467    1864    2143    2170    2389    4437 
   Size:        294     173    1397     279      27     219    2048

  Fragments arranged by size: 

                2048    1397     294     279     219     173      27
 
MslI CAynn'nnrTG

Cuts at:      0    1951    4042    4162    4402    4417    4437 
   Size:       1951    2091     120     240      15      20

  Fragments arranged by size: 

                2091    1951     240     120      20      15
 
MspA1I CmG'CkG

Cuts at:      0     387     729     740     794    1205    1469    1527    1665 
   Size:        387     342      11      54     411     264      58     138

Cuts at:   1665    2130    2409    3030    3230    3275    3429    3609    3698 
   Size:        465     279     621     200      45     154     180      89

Cuts at:   3698    3779    4042    4103    4127    4237    4437 
   Size:         81     263      61      24     110     200

  Fragments arranged by size: 

                 621     465     411     387     342     279     264     263
                 200     200     180     154     138     110      89      81
                  61      58      54      45      24      11
 
NaeI GCC'GGC

Cuts at:      0      13      17    1933    2326    4437 
   Size:         13       4    1916     393    2111

  Fragments arranged by size: 

                2111    1916     393      13       4
 
NarI GG'CG_CC

Cuts at:      0      92    1638    3980    4137    4314    4437 
   Size:         92    1546    2342     157     177     123

  Fragments arranged by size: 

                2342    1546     177     157     123      92
 
NcoI C'CATG_G

Cuts at:      0     144    1554    1653    1877    3306    3771    4163    4437 
   Size:        144    1410      99     224    1429     465     392     274

  Fragments arranged by size: 

                1429    1410     465     392     274     224     144      99
            
 
NgoMIV G'CCGG_C

Cuts at:      0      11      15    1931    2324    4437 
   Size:         11       4    1916     393    2113

  Fragments arranged by size: 

                2113    1916     393      11       4
 
NspI r_CATG'y

Cuts at:      0     327    1229    2178    4089    4437 
   Size:        327     902     949    1911     348

  Fragments arranged by size: 

                1911     949     902     348     327
 
PasI CC'CwG_GG

Cuts at:      0      97     171     318    2010    2100    2904    3786    4437 
   Size:         97      74     147    1692      90     804     882     651

  Fragments arranged by size: 

                1692     882     804     651     147      97      90      74
            
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     887    1266    2118    3141    3792    4437 
   Size:        887     379     852    1023     651     645

  Fragments arranged by size: 

                1023     887     852     651     645     379
 
PfoI T'CCnGG_A

Cuts at:      0     113    1421    2495    3818    3914    4437 
   Size:        113    1308    1074    1323      96     523

  Fragments arranged by size: 

                1323    1308    1074     523     113      96
 
PmlI CAC'GTG

Cuts at:      0    2534    2561    4437 
   Size:       2534      27    1876

  Fragments arranged by size: 

                2534    1876      27
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1891    1923    2497    2529    2986    3018    4437 
   Size:       1891      32     574      32     457      32    1419

  Fragments arranged by size: 

                1891    1419     574     457      32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     495    1755    4437 
   Size:        495    1260    2682

  Fragments arranged by size: 

                2682    1260     495
 
PsiI TTA'TAA

Cuts at:      0    2722    4437 
   Size:       2722    1715
 
PspOMI G'GGCC_C

Cuts at:      0       4     404    1455    1916    2123    2642    3742    4063 
   Size:          4     400    1051     461     207     519    1100     321

Cuts at:   4063    4077    4437 
   Size:         14     360

  Fragments arranged by size: 

                1100    1051     519     461     400     360     321     207
                  14       4
 
PstI C_TGCA'G

Cuts at:      0     155    1054    1190    1349    1754    2075    2381    3716 
   Size:        155     899     136     159     405     321     306    1335

Cuts at:   3716    3956    4437 
   Size:        240     481

  Fragments arranged by size: 

                1335     899     481     405     321     306     240     159
                 155     136
 
PvuI CG_AT'CG

Cuts at:      0    3285    4437 
   Size:       3285    1152
 
PvuII CAG'CTG

Cuts at:      0     387     729     740     794    1205    1527    1665    2130 
   Size:        387     342      11      54     411     322     138     465

Cuts at:   2130    2409    3030    3230    3698    3779    4042    4103    4437 
   Size:        279     621     200     468      81     263      61     334

  Fragments arranged by size: 

                 621     468     465     411     387     342     334     322
                 279     263     200     138      81      61      54      11
            
 
SacI G_AGCT'C

Cuts at:      0    3389    4437 
   Size:       3389    1048
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2077    4437 
   Size:       2077    2360
 
SbfI CC_TGCA'GG

Cuts at:      0    1190    1754    4437 
   Size:       1190     564    2683

  Fragments arranged by size: 

                2683    1190     564
 
ScaI AGT'ACT

Cuts at:      0    1735    4437 
   Size:       1735    2702
 
SexAI A'CCwGG_T

Cuts at:      0    1247    4437 
   Size:       1247    3190
 
SfcI C'TryA_G

Cuts at:      0     151    1050    1186    1345    1623    1750    2071    2377 
   Size:        151     899     136     159     278     127     321     306

Cuts at:   2377    2897    3712    3952    4353    4437 
   Size:        520     815     240     401      84

  Fragments arranged by size: 

                 899     815     520     401     321     306     278     240
                 159     151     136     127      84
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      12    1463    2104    4437 
   Size:         12    1451     641    2333

  Fragments arranged by size: 

                2333    1451     641      12
 
SfoI GGC'GCC

Cuts at:      0      93    1639    3981    4138    4315    4437 
   Size:         93    1546    2342     157     177     122

  Fragments arranged by size: 

                2342    1546     177     157     122      93
 
SmaI CCC'GGG

Cuts at:      0    1833    2641    3377    4070    4437 
   Size:       1833     808     736     693     367

  Fragments arranged by size: 

                1833     808     736     693     367
 
SmlI C'TyrA_G

Cuts at:      0     439     949    2468    4437 
   Size:        439     510    1519    1969

  Fragments arranged by size: 

                1969    1519     510     439
 
SnaBI TAC'GTA

Cuts at:      0    3666    4437 
   Size:       3666     771
 
SphI G_CATG'C

Cuts at:      0    4089    4437 
   Size:       4089     348
 
StuI AGG'CCT

Cuts at:      0     784    1696    3508    4437 
   Size:        784     912    1812     929

  Fragments arranged by size: 

                1812     929     912     784
 
StyI C'CwwG_G

Cuts at:      0     144    1554    1653    1877    3306    3771    4163    4437 
   Size:        144    1410      99     224    1429     465     392     274

  Fragments arranged by size: 

                1429    1410     465     392     274     224     144      99
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     367     661    1405    1825    2373    3129    3517    4156 
   Size:        367     294     744     420     548     756     388     639

Cuts at:   4156    4437 
   Size:        281

  Fragments arranged by size: 

                 756     744     639     548     420     388     367     294
                 281
 
TatI w'GTAC_w

Cuts at:      0    1733    1805    2507    2783    4437 
   Size:       1733      72     702     276    1654

  Fragments arranged by size: 

                1733    1654     702     276      72
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1303    1335    1573    1605    2761    2793    2806    2838 
   Size:       1303      32     238      32    1156      32      13      32

Cuts at:   2838    3121    3153    4437 
   Size:        283      32    1284

  Fragments arranged by size: 

                1303    1284    1156     283     238      32      32      32
                  32      32      13
 
Tth111I GACn'n_nGTC

Cuts at:      0    1249    1441    4437 
   Size:       1249     192    2996

  Fragments arranged by size: 

                2996    1249     192
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     276     315     460    1134    1595    3234    3892    4161 
   Size:        276      39     145     674     461    1639     658     269

Cuts at:   4161    4437 
   Size:        276

  Fragments arranged by size: 

                1639     674     658     461     276     276     269     145
                  39
 
XmaI C'CCGG_G

Cuts at:      0    1831    2639    3375    4068    4437 
   Size:       1831     808     736     693     369

  Fragments arranged by size: 

                1831     808     736     693     369
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AfeI     AhdI     AleI     AlfI
     AloI    AlwNI     ApaI    ApaLI     ApoI     AvaI     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrBI    BsrFI    BsrGI
    BssSI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI   DraIII
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI     FseI     FspI    HaeII    Hin4I  HindIII     KasI     KpnI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NspI     PasI    PflMI     PfoI     PmlI     PpiI    PpuMI     PsiI
   PspOMI     PstI     PvuI    PvuII     SacI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SmaI     SmlI    SnaBI     SphI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AclI    AflII   AflIII     AgeI     AscI     AseI    AsiSI
    AvrII    BciVI     BclI    BfrBI     BmtI    BsaBI    BsiWI    BsmBI
    BspEI    BspHI    BsrDI   BssHII   BstAPI    BstBI  BstZ17I   Bsu36I
     ClaI    CspCI     DraI     DrdI    EcoRV     FalI    FspAI   HincII
     HpaI     MfeI     MluI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PciI     PmeI    PshAI    PspXI     PsrI    RsrII    SacII
     SalI    SanDI     SgfI    SgrAI     SpeI     SrfI     SspI     SwaI
     XbaI     XhoI     XmnI     ZraI
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