################################## # NO CUT ENZYMES ( 139 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflIII AgeI AhdI AocI Aor51HI ApaI AscI Asp718I AspEI AspI AvaI AvrII BalI BamHI BanI BbeI BbrPI BbsI BcgI BclI BcnI BfaI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspLU11I BsrBI BsrFI BssHII Bst1107I BstAPI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoT22I FauI FbaI FseI HaeIII HapII HincII HindII HindIII HpaI HpaII HphI KasI KpnI KspI MaeI MamI MfeI MluI MluNI MroI MscI MspA1I MspI MunI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SpeI Sse8387I StuI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AcsI 219 GAATTC [GA]^AATT[CT] AcyI 1318 GACGCC G[GA]^CG[CT]C AfaI 481 GTAC GT^AC AflII 174 CTTAAG C^TTAAG Alw44I 1482 GTGCAC G^TGCAC AlwNI 710 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 1482 GTGCAC G^TGCAC ApoI 219 GAATTC [GA]^AATT[CT] AseI 636 ATTAAT AT^TAAT AsnI 636 ATTAAT AT^TAAT AspHI 1482 GTGCAC G[AT]GC[AT]^C AviII 1224 TGCGCA TGC^GCA BbvCI 154 CCTCAGC CCTCAGC(-5/-2) BbvI 1017 GCAGC GCAGC(8/12) BfrI 174 CTTAAG C^TTAAG BpmI 1031 CTGGAG CTGGAG(16/14) BsaAI 1488 TACGTA [CT]AC^GT[GA] BsaHI 1318 GACGCC G[GA]^CG[CT]C BsgI 869 CTGCAC GTGCAG(16/14) BsiHKAI 1482 GTGCAC G[AT]GC[AT]^C Bsp1407I 480 TGTACA T^GTACA BspHI 60 TCATGA T^CATGA BspMI 1478 GCAGGT ACCTGC(4/8) BsrDI 1409 CATTGC GCAATG(2/0) BsrGI 480 TGTACA T^GTACA BssSI 1105 CTCGTG CACGAG(-5/-1) BstBI 213 TTCGAA TT^CGAA BstF5I 408 GGATG GGATG(2/0) DraI 1539 TTTAAA TTT^AAA DraIII 987 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 355 CCATGG C^C[GA][CT]GG EcoRI 219 GAATTC G^AATTC EcoRV 1026 GATATC GAT^ATC EcoT14I 355 CCATGG C^C[AT][AT]GG FokI 408 GGATG GGATG(9/13) FspI 1224 TGCGCA TGC^GCA HaeII 461 GGCGCT [GA]GCGC^[CT] Hin1I 1318 GACGCC G[GA]^CG[CT]C MslI 1042 CATACCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 355 CCATGG C^CATGG NspV 213 TTCGAA TT^CGAA PflMI 355 CCATGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 636 ATTAAT AT^TAAT RcaI 60 TCATGA T^CATGA RsaI 481 GTAC GT^AC SfuI 213 TTCGAA TT^CGAA SnaBI 1488 TACGTA TAC^GTA SphI 1278 GCATGC GCATG^C SspBI 480 TGTACA T^GTACA SspI 184 AATATT AAT^ATT StyI 355 CCATGG C^C[AT][AT]GG TseI 1017 GCAGC G^C[AT]GC Van91I 355 CCATGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccII 1465 CGCG CG^CG 1467 CGCG AvaII 898 GGACC G^G[AT]CC 1158 GGACC BanII 720 GAGCCC G[GA]GC[CT]^C 1507 GGGCTC BciVI 546 GGATAC GTATCC(6/5) 978 GGATAC BsaJI 309 CCCAGG C^C[ACGT][ACGT]GG 355 CCATGG BseRI 152 CTCCTC GAGGAG(10/8) 1344 CTCCTC BsmAI 143 GAGAC GTCTC(1/5) 1034 GAGAC BssKI 310 CCAGG ^CC[ACGT]GG 913 CCTGG BstNI 310 CCAGG CC^[AT]GG 913 CCTGG BstUI 1465 CGCG CG^CG 1467 CGCG BstYI 1270 AGATCC [GA]^GATC[CT] 1380 AGATCC Cfr13I 898 GGACC G^G[ACGT]CC 1158 GGACC EcoRII 310 CCAGG ^CC[AT]GG 913 CCTGG HgaI 1318 GACGC GACGC(5/10) 1020 GCGTC MaeII 1204 ACGT A^CGT 1489 ACGT MflI 1270 AGATCC [GA]^GATC[CT] 1380 AGATCC MvaI 310 CCAGG CC^[AT]GG 913 CCTGG MvnI 1465 CGCG CG^CG 1467 CGCG NlaIV 897 GGGACC GG[ACGT]^[ACGT]CC 1508 GGCTCC PstI 1450 CTGCAG CTGCA^G 1476 CTGCAG Sau96I 898 GGACC G^G[ACGT]CC 1158 GGACC ScrFI 310 CCAGG CC^[ACGT]GG 913 CCTGG SfaNI 409 GATGC GCATC(5/9) 278 GCATC SmlI 174 CTTAAG C^T[CT][GA]AG 643 CTTGAG Tsp45I 965 GTGAC ^GT[GC]AC 1002 GTCAC XhoII 1270 AGATCC [GA]^GATC[CT] 1380 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 40 enzymes) # ######################################### AciI AluI AlwI Asp700I BmyI BsiYI BslI BsmFI Bsp1286I BsrI Cac8I CfoI DdeI DpnII EarI Fnu4HI HhaI HinP1I HinfI ItaI Ksp632I MaeIII MboI MboII MnlI MseI MwoI NdeII NlaIII NspI PleI Sau3AI SfcI TaqI TfiI Tru9I Tsp509I TspRI TthHB8I XmnI