(Circular) (Six-base) MAPSORT of: pF1KA0671.seq Check: 9222 from: 1 to: 4721 pF1KA0671 4721 bp With 182 enzymes: SgfI * June 20, 2008 10:20 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 1577 1577 Size: 4721 AccI GT'mk_AC Cuts at: 1759 1759 Size: 4721 Acc65I G'GTAC_C Cuts at: 1737 1737 Size: 4721 AclI AA'CG_TT Cuts at: 4561 4561 Size: 4721 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1325 2546 2978 3725 3993 1325 Size: 1221 432 747 268 2053 Fragments arranged by size: 2053 1221 747 432 268 AflII C'TTAA_G Cuts at: 282 282 Size: 4721 AflIII A'CryG_T Cuts at: 2073 3177 4154 2073 Size: 1104 977 2640 Fragments arranged by size: 2640 1104 977 AgeI A'CCGG_T Cuts at: 3109 3109 Size: 4721 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1333 1333 Size: 4721 AlwNI CAG_nnn'CTG Cuts at: 823 2026 3593 823 Size: 1203 1567 1951 Fragments arranged by size: 1951 1567 1203 ApaLI G'TGCA_C Cuts at: 1590 3491 1590 Size: 1901 2820 ApoI r'AATT_y Cuts at: 327 1725 4074 4330 327 Size: 1398 2349 256 718 Fragments arranged by size: 2349 1398 718 256 AseI AT'TA_AT Cuts at: 20 745 20 Size: 725 3996 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4721 AvaI C'yCGr_G Cuts at: 1741 1741 Size: 4721 BamHI G'GATC_C Cuts at: 1746 1746 Size: 4721 BanI G'GyrC_C Cuts at: 1737 2151 2399 2434 1737 Size: 414 248 35 4024 Fragments arranged by size: 4024 414 248 35 BanII G_rGCy'C Cuts at: 832 1619 1735 2765 832 Size: 787 116 1030 2788 Fragments arranged by size: 2788 1030 787 116 BbeI G_GCGC'C Cuts at: 2155 2403 2155 Size: 248 4473 BbvCI CC'TCA_GC Cuts at: 263 263 Size: 4721 BciVI GTATCCnnnnn_n' Cuts at: 648 1080 2619 3380 4721 648 Size: 432 1539 761 1341 648 Fragments arranged by size: 1539 1341 761 648 432 BclI T'GATC_A Cuts at: 2241 2241 Size: 4721 BglII A'GATC_T Cuts at: 2236 4273 2236 Size: 2037 2684 BlpI GC'TnA_GC Cuts at: 1833 1833 Size: 4721 Bme1580I G_kGCm'C Cuts at: 1594 2346 2439 3495 1594 Size: 752 93 1056 2820 Fragments arranged by size: 2820 1056 752 93 BmrI ACTGGGnnnn_n' Cuts at: 316 1012 1263 1487 1933 2014 2349 4493 316 Size: 696 251 224 446 81 335 2144 544 Fragments arranged by size: 2144 696 544 446 335 251 224 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3088 3088 Size: 4721 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1160 1160 Size: 4721 Bpu10I CC'TnA_GC Cuts at: 263 1321 263 Size: 1058 3663 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 772 1897 2776 3268 3566 772 Size: 1125 879 492 298 1927 Fragments arranged by size: 1927 1125 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 4425 4425 Size: 4721 BsaAI yAC'GTr Cuts at: 1598 2074 1598 Size: 476 4245 BsaBI GATnn'nnATC Cuts at: 6 2235 4272 6 Size: 2229 2037 455 Fragments arranged by size: 2229 2037 455 BsaHI Gr'CG_yC Cuts at: 1427 2152 2400 3102 4598 1427 Size: 725 248 702 1496 1550 Fragments arranged by size: 1550 1496 725 702 248 BsaWI w'CCGG_w Cuts at: 1910 2431 3109 3383 3530 3941 1910 Size: 521 678 274 147 411 2690 Fragments arranged by size: 2690 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 190 220 190 Size: 30 4691 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 251 1443 251 Size: 1192 3529 BseYI C'CCAG_C Cuts at: 2146 2904 3481 3977 2146 Size: 758 577 496 2890 Fragments arranged by size: 2890 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 962 962 Size: 4721 BsiEI CG_ry'CG Cuts at: 104 2263 2309 3517 104 Size: 2159 46 1208 1308 Fragments arranged by size: 2159 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 1594 1735 2513 2703 3495 1594 Size: 141 778 190 792 2820 Fragments arranged by size: 2820 792 778 190 141 Bsp1286I G_dGCh'C Cuts at: 832 1594 1619 1735 2346 2439 2513 2703 2765 Size: 762 25 116 611 93 74 190 62 Cuts at: 2765 3495 832 Size: 730 2058 Fragments arranged by size: 2058 762 730 611 190 116 93 74 62 25 BspEI T'CCGG_A Cuts at: 3941 3941 Size: 4721 BspHI T'CATG_A Cuts at: 168 3897 168 Size: 3729 992 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1577 1771 1985 2287 2668 1577 Size: 194 214 302 381 3630 Fragments arranged by size: 3630 381 302 214 194 BsrBI CCG'CTC Cuts at: 50 3013 3067 4551 4716 50 Size: 2963 54 1484 165 55 Fragments arranged by size: 2963 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1516 2633 1516 Size: 1117 3604 BsrFI r'CCGG_y Cuts at: 2719 2900 3109 2719 Size: 181 209 4331 Fragments arranged by size: 4331 209 181 BsrGI T'GTAC_A Cuts at: 588 588 Size: 4721 BssSI C'ACGA_G Cuts at: 1213 2992 3350 1213 Size: 1779 358 2584 Fragments arranged by size: 2584 1779 358 BstAPI GCAn_nnn'nTGC Cuts at: 2098 2098 Size: 4721 BstBI TT'CG_AA Cuts at: 322 3082 322 Size: 2760 1961 BstYI r'GATC_y Cuts at: 1 1378 1488 1746 2213 2236 2571 2817 3829 Size: 1377 110 258 467 23 335 246 1012 Cuts at: 3829 3915 3927 4273 1 Size: 86 12 346 449 Fragments arranged by size: 1377 1012 467 449 346 335 258 246 110 86 23 12 BtgI C'CryG_G Cuts at: 107 463 107 Size: 356 4365 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2671 2852 3015 2671 Size: 181 163 4377 Fragments arranged by size: 4377 181 163 BtsI GCAGTG_nn' Cuts at: 1206 1408 1964 1206 Size: 202 556 3963 Fragments arranged by size: 3963 556 202 DraI TTT'AAA Cuts at: 1649 1720 1649 Size: 71 4650 DraIII CAC_nnn'GTG Cuts at: 1100 1100 Size: 4721 DrdI GACnn_nn'nnGTC Cuts at: 2427 3285 2427 Size: 858 3863 EaeI y'GGCC_r Cuts at: 2306 2480 2871 2898 4243 2306 Size: 174 391 27 1345 2784 Fragments arranged by size: 2784 1345 391 174 27 EagI C'GGCC_G Cuts at: 2306 2306 Size: 4721 EarI CTCTTCn'nnn_ Cuts at: 166 432 1076 1354 2744 2954 166 Size: 266 644 278 1390 210 1933 Fragments arranged by size: 1933 1390 644 278 266 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1354 2929 3239 3385 4531 1354 Size: 1575 310 146 1146 1544 Fragments arranged by size: 1575 1544 1146 310 146 EcoICRI GAG'CTC Cuts at: 1733 1733 Size: 4721 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1160 1325 2546 2978 3725 3993 1160 Size: 165 1221 432 747 268 1888 Fragments arranged by size: 1888 1221 747 432 268 165 EcoO109I rG'GnC_Cy Cuts at: 1860 1860 Size: 4721 EcoRI G'AATT_C Cuts at: 327 1725 327 Size: 1398 3323 EcoRV GAT'ATC Cuts at: 1136 1136 Size: 4721 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 726 913 2740 726 Size: 187 1827 2707 Fragments arranged by size: 2707 1827 187 FspI TGC'GCA Cuts at: 1334 2502 1334 Size: 1168 3553 HaeII r_GCGC'y Cuts at: 573 2155 2403 3425 4404 573 Size: 1582 248 1022 979 890 Fragments arranged by size: 1582 1022 979 890 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 190 222 433 465 1688 1720 2563 2593 2595 Size: 32 211 32 1223 32 843 30 2 Cuts at: 2595 2625 190 Size: 30 2286 Fragments arranged by size: 2286 1223 843 211 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 1760 1760 Size: 4721 KasI G'GCGC_C Cuts at: 2151 2399 2151 Size: 248 4473 KpnI G_GTAC'C Cuts at: 1741 1741 Size: 4721 MluI A'CGCG_T Cuts at: 4154 4154 Size: 4721 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 233 1670 3392 3576 233 Size: 1437 1722 184 1378 Fragments arranged by size: 1722 1437 1378 184 MscI TGG'CCA Cuts at: 2482 2482 Size: 4721 MslI CAynn'nnrTG Cuts at: 1154 2710 2837 1154 Size: 1556 127 3038 Fragments arranged by size: 3038 1556 127 MspA1I CmG'CkG Cuts at: 1832 2146 2506 3519 3764 1832 Size: 314 360 1013 245 2789 Fragments arranged by size: 2789 1013 360 314 245 NaeI GCC'GGC Cuts at: 2902 2902 Size: 4721 NarI GG'CG_CC Cuts at: 2152 2400 2152 Size: 248 4473 NcoI C'CATG_G Cuts at: 107 463 107 Size: 356 4365 NgoMIV G'CCGG_C Cuts at: 2900 2900 Size: 4721 NspI r_CATG'y Cuts at: 460 1390 1506 1774 2274 3181 460 Size: 930 116 268 500 907 2000 Fragments arranged by size: 2000 930 907 500 268 116 PciI A'CATG_T Cuts at: 3177 3177 Size: 4721 PflMI CCAn_nnn'nTGG Cuts at: 469 469 Size: 4721 PfoI T'CCnGG_A Cuts at: 1020 1020 Size: 4721 PmeI GTTT'AAAC Cuts at: 1720 1720 Size: 4721 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3860 3892 3860 Size: 32 4689 PsiI TTA'TAA Cuts at: 1484 1664 1484 Size: 180 4541 PstI C_TGCA'G Cuts at: 1562 1588 1768 2453 1562 Size: 26 180 685 3830 Fragments arranged by size: 3830 685 180 26 PvuI CG_AT'CG Cuts at: 104 104 Size: 4721 PvuII CAG'CTG Cuts at: 2146 2506 2146 Size: 360 4361 RsrII CG'GwC_CG Cuts at: 2916 2916 Size: 4721 SacI G_AGCT'C Cuts at: 1735 1735 Size: 4721 SalI G'TCGA_C Cuts at: 1758 1758 Size: 4721 SapI GCTCTTCn'nnn_ Cuts at: 2744 2954 2744 Size: 210 4511 SbfI CC_TGCA'GG Cuts at: 1768 1768 Size: 4721 ScaI AGT'ACT Cuts at: 4238 4238 Size: 4721 SfcI C'TryA_G Cuts at: 33 620 875 1558 1584 1764 2449 3442 3633 Size: 587 255 683 26 180 685 993 191 Cuts at: 3633 33 Size: 1121 Fragments arranged by size: 1121 993 685 683 587 255 191 180 26 SfoI GGC'GCC Cuts at: 2153 2401 2153 Size: 248 4473 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4721 SmaI CCC'GGG Cuts at: 1743 1743 Size: 4721 SmlI C'TyrA_G Cuts at: 282 751 1876 2791 3283 3545 282 Size: 469 1125 915 492 262 1458 Fragments arranged by size: 1458 1125 915 492 469 262 SnaBI TAC'GTA Cuts at: 1598 1598 Size: 4721 SpeI A'CTAG_T Cuts at: 67 67 Size: 4721 SphI G_CATG'C Cuts at: 1390 1774 2274 1390 Size: 384 500 3837 Fragments arranged by size: 3837 500 384 SspI AAT'ATT Cuts at: 294 294 Size: 4721 StyI C'CwwG_G Cuts at: 107 463 1855 2209 107 Size: 356 1392 354 2619 Fragments arranged by size: 2619 1392 356 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 154 2304 2437 2896 3105 154 Size: 2150 133 459 209 1770 Fragments arranged by size: 2150 1770 459 209 133 TatI w'GTAC_w Cuts at: 588 4236 588 Size: 3648 1073 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4238 4270 4238 Size: 32 4689 Tth111I GACn'n_nGTC Cuts at: 2518 2518 Size: 4721 XbaI T'CTAG_A Cuts at: 1752 1752 Size: 4721 XcmI CCAnnnn_n'nnnnTGG Cuts at: 114 2216 114 Size: 2102 2619 XmaI C'CCGG_G Cuts at: 1741 1741 Size: 4721 XmnI GAAnn'nnTTC Cuts at: 136 160 441 1286 136 Size: 24 281 845 3571 Fragments arranged by size: 3571 845 281 24 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI PpiI PsiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII AfeI AhdI AleI AloI ApaI AscI AvrII BaeI BbsI BcgI BfrBI BglI BmgBI BmtI BsiWI BsmI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI EcoNI FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PmlI PpuMI PshAI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SrfI StuI SwaI XhoI ZraI