(Linear) (Six-base) MAPSORT of: KIAA0540.seq Check: 4347 from: 1 to: 8262 KIAA0540.orf With 182 enzymes: SgfI * July 18, 2007 13:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 142 1238 1613 1663 3006 3981 5939 8262 Size: 142 1096 375 50 1343 975 1958 2323 Fragments arranged by size: 2323 1958 1343 1096 975 375 142 50 AccI GT'mk_AC Cuts at: 0 2093 5837 6926 8262 Size: 2093 3744 1089 1336 Fragments arranged by size: 3744 2093 1336 1089 Acc65I G'GTAC_C Cuts at: 0 1773 8262 Size: 1773 6489 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 100 2055 2747 2804 2866 3968 4628 5386 Size: 100 1955 692 57 62 1102 660 758 Cuts at: 5386 5539 7033 7281 7287 8262 Size: 153 1494 248 6 975 Fragments arranged by size: 1955 1494 1102 975 758 692 660 248 153 100 62 57 6 AfeI AGC'GCT Cuts at: 0 4568 4978 5565 8262 Size: 4568 410 587 2697 Fragments arranged by size: 4568 2697 587 410 AflIII A'CryG_T Cuts at: 0 3131 5183 6317 8262 Size: 3131 2052 1134 1945 Fragments arranged by size: 3131 2052 1945 1134 AhdI GACnn_n'nnGTC Cuts at: 0 2777 5208 6318 7001 8262 Size: 2777 2431 1110 683 1261 Fragments arranged by size: 2777 2431 1261 1110 683 AleI CACnn'nnGTG Cuts at: 0 1465 1673 4342 4453 5359 8262 Size: 1465 208 2669 111 906 2903 Fragments arranged by size: 2903 2669 1465 906 208 111 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2548 3562 4995 7676 8119 8262 Size: 2548 1014 1433 2681 443 143 Fragments arranged by size: 2681 2548 1433 1014 443 143 AlwNI CAG_nnn'CTG Cuts at: 0 78 1374 1548 1678 1801 2082 2796 2851 Size: 78 1296 174 130 123 281 714 55 Cuts at: 2851 3006 3165 3627 4629 4924 4960 4969 5304 Size: 155 159 462 1002 295 36 9 335 Cuts at: 5304 6798 7011 7104 7344 7644 8262 Size: 1494 213 93 240 300 618 Fragments arranged by size: 1494 1296 1002 714 618 462 335 300 295 281 240 213 174 159 155 130 123 93 78 55 36 9 ApaI G_GGCC'C Cuts at: 0 2477 2946 3041 7875 8262 Size: 2477 469 95 4834 387 Fragments arranged by size: 4834 2477 469 387 95 ApaLI G'TGCA_C Cuts at: 0 1968 2463 8262 Size: 1968 495 5799 Fragments arranged by size: 5799 1968 495 ApoI r'AATT_y Cuts at: 0 535 6652 8262 Size: 535 6117 1610 Fragments arranged by size: 6117 1610 535 AscI GG'CGCG_CC Cuts at: 0 5332 8262 Size: 5332 2930 AvaI C'yCGr_G Cuts at: 0 314 1046 1057 5662 7562 8226 8262 Size: 314 732 11 4605 1900 664 36 Fragments arranged by size: 4605 1900 732 664 314 36 11 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 3727 3760 8262 Size: 3727 33 4502 Fragments arranged by size: 4502 3727 33 BanI G'GyrC_C Cuts at: 0 94 458 1050 1709 1773 2060 2359 2487 Size: 94 364 592 659 64 287 299 128 Cuts at: 2487 2609 2677 4992 5129 5261 5420 5526 5900 Size: 122 68 2315 137 132 159 106 374 Cuts at: 5900 6469 7252 7362 7623 7783 7987 8262 Size: 569 783 110 261 160 204 275 Fragments arranged by size: 2315 783 659 592 569 374 364 299 287 275 261 204 160 159 137 132 128 122 110 106 94 68 64 BanII G_rGCy'C Cuts at: 0 880 1100 2477 2522 2946 3041 4262 5969 Size: 880 220 1377 45 424 95 1221 1707 Cuts at: 5969 6524 6625 6644 7852 7875 8262 Size: 555 101 19 1208 23 387 Fragments arranged by size: 1707 1377 1221 1208 880 555 424 387 220 101 95 45 23 19 BbeI G_GCGC'C Cuts at: 0 462 1054 2064 2613 5133 5265 5424 5904 Size: 462 592 1010 549 2520 132 159 480 Cuts at: 5904 8262 Size: 2358 Fragments arranged by size: 2520 2358 1010 592 549 480 462 159 132 BbsI GAAGACnn'nnnn_ Cuts at: 0 959 3905 3984 4778 5102 6345 6888 8262 Size: 959 2946 79 794 324 1243 543 1374 Fragments arranged by size: 2946 1374 1243 959 794 543 324 79 BbvCI CC'TCA_GC Cuts at: 0 982 1649 3026 3760 4443 5455 6337 7043 Size: 982 667 1377 734 683 1012 882 706 Cuts at: 7043 8262 Size: 1219 Fragments arranged by size: 1377 1219 1012 982 882 734 706 683 667 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 4372 4406 8115 8149 8160 8194 8262 Size: 4372 34 3709 34 11 34 68 Fragments arranged by size: 4372 3709 68 34 34 34 11 BciVI GTATCCnnnnn_n' Cuts at: 0 1836 2176 8262 Size: 1836 340 6086 Fragments arranged by size: 6086 1836 340 BclI T'GATC_A Cuts at: 0 7730 8262 Size: 7730 532 BglI GCCn_nnn'nGGC Cuts at: 0 138 1484 1525 2239 2440 2618 3841 4157 Size: 138 1346 41 714 201 178 1223 316 Cuts at: 4157 5037 6607 6883 8262 Size: 880 1570 276 1379 Fragments arranged by size: 1570 1379 1346 1223 880 714 316 276 201 178 138 41 BglII A'GATC_T Cuts at: 0 3720 8262 Size: 3720 4542 BlpI GC'TnA_GC Cuts at: 0 1394 2521 3349 3977 5705 7492 7851 8262 Size: 1394 1127 828 628 1728 1787 359 411 Fragments arranged by size: 1787 1728 1394 1127 828 628 411 359 Bme1580I G_kGCm'C Cuts at: 0 314 825 1324 1972 2467 2477 2682 2946 Size: 314 511 499 648 495 10 205 264 Cuts at: 2946 3041 5531 6022 7255 7875 7990 8262 Size: 95 2490 491 1233 620 115 272 Fragments arranged by size: 2490 1233 648 620 511 499 495 491 314 272 264 205 115 95 10 BmgBI CAC'GTC Cuts at: 0 3327 4361 6540 8262 Size: 3327 1034 2179 1722 Fragments arranged by size: 3327 2179 1722 1034 BmrI ACTGGGnnnn_n' Cuts at: 0 1576 2265 2585 4894 5163 5415 6022 7446 Size: 1576 689 320 2309 269 252 607 1424 Cuts at: 7446 7989 8262 Size: 543 273 Fragments arranged by size: 2309 1576 1424 689 607 543 320 273 269 252 BmtI G_CTAG'C Cuts at: 0 1519 7165 8262 Size: 1519 5646 1097 Fragments arranged by size: 5646 1519 1097 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2172 4998 5480 5631 8262 Size: 2172 2826 482 151 2631 Fragments arranged by size: 2826 2631 2172 482 151 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 148 244 295 653 726 1543 1861 2416 Size: 148 96 51 358 73 817 318 555 Cuts at: 2416 2716 3604 3733 3901 4127 4203 4258 4510 Size: 300 888 129 168 226 76 55 252 Cuts at: 4510 4522 4894 5359 5731 5836 6637 6694 6697 Size: 12 372 465 372 105 801 57 3 Cuts at: 6697 6820 6988 8137 8262 Size: 123 168 1149 125 Fragments arranged by size: 1149 888 817 801 555 465 372 372 358 318 300 252 226 168 168 148 129 125 123 105 96 76 73 57 55 51 12 3 Bpu10I CC'TnA_GC Cuts at: 0 879 982 1649 1917 3026 3578 3760 4443 Size: 879 103 667 268 1109 552 182 683 Cuts at: 4443 5455 6148 6337 7043 8262 Size: 1012 693 189 706 1219 Fragments arranged by size: 1219 1109 1012 879 706 693 683 667 552 268 189 182 103 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 229 850 5983 6122 7099 8262 Size: 229 621 5133 139 977 1163 Fragments arranged by size: 5133 1163 977 621 229 139 BsaI GGTCTCn'nnnn_ Cuts at: 0 2626 3622 5707 6048 8262 Size: 2626 996 2085 341 2214 Fragments arranged by size: 2626 2214 2085 996 341 BsaAI yAC'GTr Cuts at: 0 1726 4800 5186 6318 7574 8103 8262 Size: 1726 3074 386 1132 1256 529 159 Fragments arranged by size: 3074 1726 1256 1132 529 386 159 BsaHI Gr'CG_yC Cuts at: 0 459 926 1051 1133 2061 2610 3182 5130 Size: 459 467 125 82 928 549 572 1948 Cuts at: 5130 5262 5421 5901 8262 Size: 132 159 480 2361 Fragments arranged by size: 2361 1948 928 572 549 480 467 459 159 132 125 82 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 4 34 4125 4155 6793 6799 6823 6829 Size: 4 30 4091 30 2638 6 24 6 Cuts at: 6829 8262 Size: 1433 Fragments arranged by size: 4091 2638 1433 30 30 24 6 6 4 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 160 791 2148 2527 4861 5623 5890 6895 Size: 160 631 1357 379 2334 762 267 1005 Cuts at: 6895 8262 Size: 1367 Fragments arranged by size: 2334 1367 1357 1005 762 631 379 267 160 BseYI C'CCAG_C Cuts at: 0 1753 2280 2300 2406 2966 2999 3662 4430 Size: 1753 527 20 106 560 33 663 768 Cuts at: 4430 4434 4559 5034 5912 6059 6345 6401 6438 Size: 4 125 475 878 147 286 56 37 Cuts at: 6438 7248 7983 8262 Size: 810 735 279 Fragments arranged by size: 1753 878 810 768 735 663 560 527 475 286 279 147 125 106 56 37 33 20 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 176 193 788 1489 1706 1816 2693 2935 Size: 176 17 595 701 217 110 877 242 Cuts at: 2935 3286 3349 3403 3454 4041 4865 5386 6520 Size: 351 63 54 51 587 824 521 1134 Cuts at: 6520 6803 6891 7445 7657 7990 8262 Size: 283 88 554 212 333 272 Fragments arranged by size: 1134 877 824 701 595 587 554 521 351 333 283 272 242 217 212 176 110 88 63 54 51 17 BsiEI CG_ry'CG Cuts at: 0 1946 3531 5771 6576 8039 8262 Size: 1946 1585 2240 805 1463 223 Fragments arranged by size: 2240 1946 1585 1463 805 223 BsiHKAI G_wGCw'C Cuts at: 0 880 1731 1972 2467 2522 2990 3155 4427 Size: 880 851 241 495 55 468 165 1272 Cuts at: 4427 4865 5656 5969 6644 7852 8262 Size: 438 791 313 675 1208 410 Fragments arranged by size: 1272 1208 880 851 791 675 495 468 438 410 313 241 165 55 BsmI GAATG_Cn' Cuts at: 0 573 5229 7120 8262 Size: 573 4656 1891 1142 Fragments arranged by size: 4656 1891 1142 573 BsmBI CGTCTCn'nnnn_ Cuts at: 0 607 2476 8227 8262 Size: 607 1869 5751 35 Fragments arranged by size: 5751 1869 607 35 Bsp1286I G_dGCh'C Cuts at: 0 314 825 880 1100 1324 1731 1972 2467 Size: 314 511 55 220 224 407 241 495 Cuts at: 2467 2477 2522 2682 2946 2990 3041 3155 4262 Size: 10 45 160 264 44 51 114 1107 Cuts at: 4262 4427 4865 5531 5656 5969 6022 6524 6625 Size: 165 438 666 125 313 53 502 101 Cuts at: 6625 6644 7255 7852 7875 7990 8262 Size: 19 611 597 23 115 272 Fragments arranged by size: 1107 666 611 597 511 502 495 438 407 314 313 272 264 241 224 220 165 160 125 115 114 101 55 53 51 45 44 23 19 10 BspHI T'CATG_A Cuts at: 0 1172 8262 Size: 1172 7090 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 74 142 1046 1174 1238 1613 1663 2722 Size: 74 68 904 128 64 375 50 1059 Cuts at: 2722 3006 3321 3410 3981 5325 5939 5954 7525 Size: 284 315 89 571 1344 614 15 1571 Cuts at: 7525 7629 8262 Size: 104 633 Fragments arranged by size: 1571 1344 1059 904 633 614 571 375 315 284 128 104 89 74 68 64 50 15 BsrBI CCG'CTC Cuts at: 0 15 2267 3570 3582 6158 6951 8262 Size: 15 2252 1303 12 2576 793 1311 Fragments arranged by size: 2576 2252 1311 1303 793 15 12 BsrDI GCAATG_nn' Cuts at: 0 1136 1381 6251 8262 Size: 1136 245 4870 2011 Fragments arranged by size: 4870 2011 1136 245 BsrFI r'CCGG_y Cuts at: 0 956 1350 2063 3895 6575 7178 8262 Size: 956 394 713 1832 2680 603 1084 Fragments arranged by size: 2680 1832 1084 956 713 603 394 BsrGI T'GTAC_A Cuts at: 0 1782 1892 8262 Size: 1782 110 6370 Fragments arranged by size: 6370 1782 110 BssHII G'CGCG_C Cuts at: 0 5274 5332 8257 8262 Size: 5274 58 2925 5 Fragments arranged by size: 5274 2925 58 5 BssSI C'ACGA_G Cuts at: 0 803 2755 6108 6406 6829 8262 Size: 803 1952 3353 298 423 1433 Fragments arranged by size: 3353 1952 1433 803 423 298 BstAPI GCAn_nnn'nTGC Cuts at: 0 992 1284 1374 2851 3345 3858 4815 4960 Size: 992 292 90 1477 494 513 957 145 Cuts at: 4960 4969 7344 8262 Size: 9 2375 918 Fragments arranged by size: 2375 1477 992 957 918 513 494 292 145 90 9 BstBI TT'CG_AA Cuts at: 0 5174 8262 Size: 5174 3088 BstEII G'GTnAC_C Cuts at: 0 61 1306 4618 5820 7878 8262 Size: 61 1245 3312 1202 2058 384 Fragments arranged by size: 3312 2058 1245 1202 384 61 BstXI CCAn_nnnn'nTGG Cuts at: 0 342 469 942 2056 2394 4236 6381 7441 Size: 342 127 473 1114 338 1842 2145 1060 Cuts at: 7441 7578 8061 8262 Size: 137 483 201 Fragments arranged by size: 2145 1842 1114 1060 483 473 342 338 201 137 127 BstYI r'GATC_y Cuts at: 0 3542 3720 7711 8262 Size: 3542 178 3991 551 Fragments arranged by size: 3991 3542 551 178 BstZ17I GTA'TAC Cuts at: 0 5838 8262 Size: 5838 2424 Bsu36I CC'TnA_GG Cuts at: 0 1423 2501 2552 3644 6183 6767 8262 Size: 1423 1078 51 1092 2539 584 1495 Fragments arranged by size: 2539 1495 1423 1092 1078 584 51 BtgI C'CryG_G Cuts at: 0 711 1251 1589 2972 4183 4535 4614 5390 Size: 711 540 338 1383 1211 352 79 776 Cuts at: 5390 5798 6751 7319 7357 7470 7749 8262 Size: 408 953 568 38 113 279 513 Fragments arranged by size: 1383 1211 953 776 711 568 540 513 408 352 338 279 113 79 38 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1803 3386 4397 6749 7042 8262 Size: 1803 1583 1011 2352 293 1220 Fragments arranged by size: 2352 1803 1583 1220 1011 293 BtsI GCAGTG_nn' Cuts at: 0 79 474 1375 1552 2031 2852 2923 3511 Size: 79 395 901 177 479 821 71 588 Cuts at: 3511 4106 4134 4835 5050 5256 5366 5617 5636 Size: 595 28 701 215 206 110 251 19 Cuts at: 5636 7377 7403 7670 7927 8026 8262 Size: 1741 26 267 257 99 236 Fragments arranged by size: 1741 901 821 701 595 588 479 395 267 257 251 236 215 206 177 110 99 79 71 28 26 19 ClaI AT'CG_AT Cuts at: 0 5978 8262 Size: 5978 2284 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 2277 2312 5073 5108 5648 5683 8262 Size: 2277 35 2761 35 540 35 2579 Fragments arranged by size: 2761 2579 2277 540 35 35 35 DraIII CAC_nnn'GTG Cuts at: 0 2037 2375 2628 2895 6591 7574 8262 Size: 2037 338 253 267 3696 983 688 Fragments arranged by size: 3696 2037 983 688 338 267 253 DrdI GACnn_nn'nnGTC Cuts at: 0 3331 8262 Size: 3331 4931 EaeI y'GGCC_r Cuts at: 0 2 197 862 958 1190 1618 1703 1792 Size: 2 195 665 96 232 428 85 89 Cuts at: 1792 1943 2103 2417 3362 3502 4206 4407 4547 Size: 151 160 314 945 140 704 201 140 Cuts at: 4547 4697 5768 5795 6555 7431 7676 8036 8063 Size: 150 1071 27 760 876 245 360 27 Cuts at: 8063 8078 8262 Size: 15 184 Fragments arranged by size: 1071 945 876 760 704 665 428 360 314 245 232 201 195 184 160 151 150 140 140 96 89 85 27 27 15 2 EagI C'GGCC_G Cuts at: 0 1943 5768 8036 8262 Size: 1943 3825 2268 226 Fragments arranged by size: 3825 2268 1943 226 EarI CTCTTCn'nnn_ Cuts at: 0 100 256 490 619 884 1411 3076 4313 Size: 100 156 234 129 265 527 1665 1237 Cuts at: 4313 4911 5974 8262 Size: 598 1063 2288 Fragments arranged by size: 2288 1665 1237 1063 598 527 265 234 156 129 100 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 361 1658 2985 6276 8262 Size: 361 1297 1327 3291 1986 Fragments arranged by size: 3291 1986 1327 1297 361 EcoICRI GAG'CTC Cuts at: 0 878 2520 5967 6642 7850 8262 Size: 878 1642 3447 675 1208 412 Fragments arranged by size: 3447 1642 1208 878 675 412 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 100 148 244 295 653 726 1543 1861 Size: 100 48 96 51 358 73 817 318 Cuts at: 1861 2055 2416 2716 2747 2804 2866 3604 3733 Size: 194 361 300 31 57 62 738 129 Cuts at: 3733 3901 3968 4127 4203 4258 4510 4522 4628 Size: 168 67 159 76 55 252 12 106 Cuts at: 4628 4894 5359 5386 5539 5731 5836 6637 6694 Size: 266 465 27 153 192 105 801 57 Cuts at: 6694 6697 6820 6988 7033 7281 7287 8137 8262 Size: 3 123 168 45 248 6 850 125 Fragments arranged by size: 850 817 801 738 465 361 358 318 300 266 252 248 194 192 168 168 159 153 129 125 123 106 105 100 96 76 73 67 62 57 57 55 51 48 45 31 27 12 6 3 EcoNI CCTnn'n_nnAGG Cuts at: 0 1234 2329 2953 3433 7597 8262 Size: 1234 1095 624 480 4164 665 Fragments arranged by size: 4164 1234 1095 665 624 480 EcoO109I rG'GnC_Cy Cuts at: 0 54 162 429 681 883 894 1113 1717 Size: 54 108 267 252 202 11 219 604 Cuts at: 1717 1798 1949 2456 2473 2761 2793 2926 2942 Size: 81 151 507 17 288 32 133 16 Cuts at: 2942 2943 2958 3038 3675 4008 4439 4530 4542 Size: 1 15 80 637 333 431 91 12 Cuts at: 4542 4937 5444 6303 6360 6435 6885 7641 7871 Size: 395 507 859 57 75 450 756 230 Cuts at: 7871 8262 Size: 391 Fragments arranged by size: 859 756 637 604 507 507 450 431 395 391 333 288 267 252 230 219 202 151 133 108 91 81 80 75 57 54 32 17 16 15 12 11 1 EcoRI G'AATT_C Cuts at: 0 535 6652 8262 Size: 535 6117 1610 Fragments arranged by size: 6117 1610 535 EcoRV GAT'ATC Cuts at: 0 6218 8262 Size: 6218 2044 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 913 7140 8262 Size: 913 6227 1122 Fragments arranged by size: 6227 1122 913 FspI TGC'GCA Cuts at: 0 3208 3550 5005 5321 8167 8262 Size: 3208 342 1455 316 2846 95 Fragments arranged by size: 3208 2846 1455 342 316 95 FspAI rTGC'GCAy Cuts at: 0 5005 8262 Size: 5005 3257 HaeII r_GCGC'y Cuts at: 0 462 545 1054 2064 2613 3237 3597 4570 Size: 462 83 509 1010 549 624 360 973 Cuts at: 4570 4980 5133 5265 5416 5424 5455 5495 5567 Size: 410 153 132 151 8 31 40 72 Cuts at: 5567 5904 8262 Size: 337 2358 Fragments arranged by size: 2358 1010 973 624 549 509 462 410 360 337 153 151 132 83 72 40 31 8 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1171 1203 1239 1271 2763 2795 4125 4157 Size: 1171 32 36 32 1492 32 1330 32 Cuts at: 4157 4267 4299 4921 4953 5194 5226 6304 6336 Size: 110 32 622 32 241 32 1078 32 Cuts at: 6336 6799 6831 6987 7019 7066 7098 8262 Size: 463 32 156 32 47 32 1164 Fragments arranged by size: 1492 1330 1171 1164 1078 622 463 241 156 110 47 36 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 332 8262 Size: 332 7930 HindIII A'AGCT_T Cuts at: 0 6426 8262 Size: 6426 1836 KasI G'GCGC_C Cuts at: 0 458 1050 2060 2609 5129 5261 5420 5900 Size: 458 592 1010 549 2520 132 159 480 Cuts at: 5900 8262 Size: 2362 Fragments arranged by size: 2520 2362 1010 592 549 480 458 159 132 KpnI G_GTAC'C Cuts at: 0 1777 8262 Size: 1777 6485 MfeI C'AATT_G Cuts at: 0 990 8262 Size: 990 7272 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 371 1475 3440 6245 6596 6785 7051 8040 Size: 371 1104 1965 2805 351 189 266 989 Cuts at: 8040 8262 Size: 222 Fragments arranged by size: 2805 1965 1104 989 371 351 266 222 189 MscI TGG'CCA Cuts at: 0 864 1705 1794 2419 4409 4549 4699 7433 Size: 864 841 89 625 1990 140 150 2734 Cuts at: 7433 7678 8065 8262 Size: 245 387 197 Fragments arranged by size: 2734 1990 864 841 625 387 245 197 150 140 89 MslI CAynn'nnrTG Cuts at: 0 934 1297 1465 1673 2977 2983 4342 4453 Size: 934 363 168 208 1304 6 1359 111 Cuts at: 4453 5124 5242 5359 6379 6514 7439 7505 7576 Size: 671 118 117 1020 135 925 66 71 Cuts at: 7576 7601 7702 8059 8137 8262 Size: 25 101 357 78 125 Fragments arranged by size: 1359 1304 1020 934 925 671 363 357 208 168 135 125 118 117 111 101 78 71 66 25 6 MspA1I CmG'CkG Cuts at: 0 168 207 868 1393 1442 1532 1545 1890 Size: 168 39 661 525 49 90 13 345 Cuts at: 1890 1913 2179 2740 3003 3025 3035 3228 3741 Size: 23 266 561 263 22 10 193 513 Cuts at: 3741 4185 4434 4711 4782 4951 4960 4969 5034 Size: 444 249 277 71 169 9 9 65 Cuts at: 5034 5259 5477 5781 5916 6546 6714 6864 7008 Size: 225 218 304 135 630 168 150 144 Cuts at: 7008 7042 7262 7427 7496 7751 8262 Size: 34 220 165 69 255 511 Fragments arranged by size: 661 630 561 525 513 511 444 345 304 277 266 263 255 249 225 220 218 193 169 168 168 165 150 144 135 90 71 69 65 49 39 34 23 22 13 10 9 9 NaeI GCC'GGC Cuts at: 0 2065 3897 8262 Size: 2065 1832 4365 Fragments arranged by size: 4365 2065 1832 NarI GG'CG_CC Cuts at: 0 459 1051 2061 2610 5130 5262 5421 5901 Size: 459 592 1010 549 2520 132 159 480 Cuts at: 5901 8262 Size: 2361 Fragments arranged by size: 2520 2361 1010 592 549 480 459 159 132 NcoI C'CATG_G Cuts at: 0 1251 1589 2972 4614 5390 7319 8262 Size: 1251 338 1383 1642 776 1929 943 Fragments arranged by size: 1929 1642 1383 1251 943 776 338 NgoMIV G'CCGG_C Cuts at: 0 2063 3895 8262 Size: 2063 1832 4367 Fragments arranged by size: 4367 2063 1832 NheI G'CTAG_C Cuts at: 0 1515 7161 8262 Size: 1515 5646 1101 Fragments arranged by size: 5646 1515 1101 NspI r_CATG'y Cuts at: 0 193 412 787 921 950 1320 3135 4789 Size: 193 219 375 134 29 370 1815 1654 Cuts at: 4789 4815 5241 5514 6400 8262 Size: 26 426 273 886 1862 Fragments arranged by size: 1862 1815 1654 886 426 375 370 273 219 193 134 29 26 PasI CC'CwG_GG Cuts at: 0 372 2469 2790 5806 6299 7203 8262 Size: 372 2097 321 3016 493 904 1059 Fragments arranged by size: 3016 2097 1059 904 493 372 321 PciI A'CATG_T Cuts at: 0 3131 8262 Size: 3131 5131 PflMI CCAn_nnn'nTGG Cuts at: 0 1801 2082 2628 2796 3627 4177 5304 6591 Size: 1801 281 546 168 831 550 1127 1287 Cuts at: 6591 6798 7104 7372 7839 8106 8262 Size: 207 306 268 467 267 156 Fragments arranged by size: 1801 1287 1127 831 550 546 467 306 281 268 267 207 168 156 PfoI T'CCnGG_A Cuts at: 0 1115 3878 4253 4487 5297 6356 6647 6674 Size: 1115 2763 375 234 810 1059 291 27 Cuts at: 6674 8262 Size: 1588 Fragments arranged by size: 2763 1588 1115 1059 810 375 291 234 27 PmlI CAC'GTG Cuts at: 0 1726 7574 8103 8262 Size: 1726 5848 529 159 Fragments arranged by size: 5848 1726 529 159 PpuMI rG'GwC_Cy Cuts at: 0 54 162 1113 1717 1949 2456 2761 2958 Size: 54 108 951 604 232 507 305 197 Cuts at: 2958 4542 4937 6303 6360 6435 7641 8262 Size: 1584 395 1366 57 75 1206 621 Fragments arranged by size: 1584 1366 1206 951 621 604 507 395 305 232 197 108 75 57 54 PshAI GACnn'nnGTC Cuts at: 0 3779 6028 8262 Size: 3779 2249 2234 Fragments arranged by size: 3779 2249 2234 PspOMI G'GGCC_C Cuts at: 0 2473 2942 3037 7871 8262 Size: 2473 469 95 4834 391 Fragments arranged by size: 4834 2473 469 391 95 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 6088 6120 6277 6309 8262 Size: 6088 32 157 32 1953 Fragments arranged by size: 6088 1953 157 32 32 PstI C_TGCA'G Cuts at: 0 71 268 1043 1235 1247 2330 2441 3560 Size: 71 197 775 192 12 1083 111 1119 Cuts at: 3560 4592 4830 4956 4965 4974 5045 5675 6311 Size: 1032 238 126 9 9 71 630 636 Cuts at: 6311 7665 8262 Size: 1354 597 Fragments arranged by size: 1354 1119 1083 1032 775 636 630 597 238 197 192 126 111 71 71 12 9 9 PvuI CG_AT'CG Cuts at: 0 3531 8262 Size: 3531 4731 PvuII CAG'CTG Cuts at: 0 207 868 1442 1545 1890 2740 3003 3025 Size: 207 661 574 103 345 850 263 22 Cuts at: 3025 3741 4434 4711 4782 4951 4960 4969 5781 Size: 716 693 277 71 169 9 9 812 Cuts at: 5781 5916 6546 6714 7008 7042 7262 7496 8262 Size: 135 630 168 294 34 220 234 766 Fragments arranged by size: 850 812 766 716 693 661 630 574 345 294 277 263 234 220 207 169 168 135 103 71 34 22 9 9 SacI G_AGCT'C Cuts at: 0 880 2522 5969 6644 7852 8262 Size: 880 1642 3447 675 1208 410 Fragments arranged by size: 3447 1642 1208 880 675 410 SacII CC_GC'GG Cuts at: 0 4186 7752 8262 Size: 4186 3566 510 Fragments arranged by size: 4186 3566 510 SanDI GG'GwC_CC Cuts at: 0 1949 4937 8262 Size: 1949 2988 3325 Fragments arranged by size: 3325 2988 1949 SapI GCTCTTCn'nnn_ Cuts at: 0 100 256 490 1411 3076 5974 8262 Size: 100 156 234 921 1665 2898 2288 Fragments arranged by size: 2898 2288 1665 921 234 156 100 SbfI CC_TGCA'GG Cuts at: 0 1235 2330 2441 6311 8262 Size: 1235 1095 111 3870 1951 Fragments arranged by size: 3870 1951 1235 1095 111 ScaI AGT'ACT Cuts at: 0 6232 8262 Size: 6232 2030 SexAI A'CCwGG_T Cuts at: 0 2081 6098 8262 Size: 2081 4017 2164 Fragments arranged by size: 4017 2164 2081 SfcI C'TryA_G Cuts at: 0 67 264 562 1039 1231 1243 2326 2437 Size: 67 197 298 477 192 12 1083 111 Cuts at: 2437 3216 3556 4588 4819 4826 4952 4961 4970 Size: 779 340 1032 231 7 126 9 9 Cuts at: 4970 5041 5671 6307 6923 7661 7949 8262 Size: 71 630 636 616 738 288 313 Fragments arranged by size: 1083 1032 779 738 636 630 616 477 340 313 298 288 231 197 192 126 111 71 67 12 9 9 7 SfoI GGC'GCC Cuts at: 0 460 1052 2062 2611 5131 5263 5422 5902 Size: 460 592 1010 549 2520 132 159 480 Cuts at: 5902 8262 Size: 2360 Fragments arranged by size: 2520 2360 1010 592 549 480 460 159 132 SmaI CCC'GGG Cuts at: 0 1048 7564 8262 Size: 1048 6516 698 Fragments arranged by size: 6516 1048 698 SmlI C'TyrA_G Cuts at: 0 244 829 5962 6137 7114 8262 Size: 244 585 5133 175 977 1148 Fragments arranged by size: 5133 1148 977 585 244 175 SphI G_CATG'C Cuts at: 0 787 921 4789 5514 6400 8262 Size: 787 134 3868 725 886 1862 Fragments arranged by size: 3868 1862 886 787 725 134 StuI AGG'CCT Cuts at: 0 85 1478 1930 4795 4852 5614 6188 7619 Size: 85 1393 452 2865 57 762 574 1431 Cuts at: 7619 8262 Size: 643 Fragments arranged by size: 2865 1431 1393 762 643 574 452 85 57 StyI C'CwwG_G Cuts at: 0 488 1193 1251 1589 2972 3443 4614 5390 Size: 488 705 58 338 1383 471 1171 776 Cuts at: 5390 7319 8087 8262 Size: 1929 768 175 Fragments arranged by size: 1929 1383 1171 776 768 705 488 471 338 175 58 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1279 2377 2665 2803 3106 3226 3379 3966 Size: 1279 1098 288 138 303 120 153 587 Cuts at: 3966 3975 4444 4531 4573 5082 5572 5616 6487 Size: 9 469 87 42 509 490 44 871 Cuts at: 6487 7216 7270 7350 7771 8262 Size: 729 54 80 421 491 Fragments arranged by size: 1279 1098 871 729 587 509 491 490 469 421 303 288 153 138 120 87 80 54 44 42 9 TatI w'GTAC_w Cuts at: 0 1782 1892 3128 4749 6104 6230 8262 Size: 1782 110 1236 1621 1355 126 2032 Fragments arranged by size: 2032 1782 1621 1355 1236 126 110 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2210 2242 2359 2391 3081 3113 4309 4341 Size: 2210 32 117 32 690 32 1196 32 Cuts at: 4341 4585 4617 7558 7590 8262 Size: 244 32 2941 32 672 Fragments arranged by size: 2941 2210 1196 690 672 244 117 32 32 32 32 32 32 XbaI T'CTAG_A Cuts at: 0 750 8262 Size: 750 7512 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 5444 6550 7245 8073 8262 Size: 5444 1106 695 828 189 Fragments arranged by size: 5444 1106 828 695 189 XmaI C'CCGG_G Cuts at: 0 1046 7562 8262 Size: 1046 6516 700 Fragments arranged by size: 6516 1046 700 Enzymes that do cut: AarI AccI Acc65I AcuI AfeI AflIII AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AvaI BaeI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII HindIII KasI KpnI MfeI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI PasI PciI PflMI PfoI PmlI PpuMI PshAI PspOMI PsrI PstI PvuI PvuII SacI SacII SanDI SapI SbfI ScaI SexAI SfcI SfoI SmaI SmlI SphI StuI StyI TaqII TatI TstI XbaI XcmI XmaI Enzymes that do not cut: AatII AclI AflII AgeI AloI AseI AsiSI AvrII BamHI BfrBI BsaBI BsaWI BsiWI BspEI DraI FseI HpaI MluI NdeI NotI NruI NsiI PacI PmeI PpiI PsiI PspXI RsrII SalI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI Tth111I XhoI XmnI ZraI