Restriction map for
pF1KA0520
(Circular) (Six-base) MAPSORT of: pF1KA0520.seq Check: 1358 from: 1 to: 7172
pF1KA0520 7172 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2527 3518 2527
Size: 991 6181
AatII G_ACGT'C
Cuts at: 2651 2651
Size: 7172
AccI GT'mk_AC
Cuts at: 2488 2747 4210 2488
Size: 259 1463 5450
Fragments arranged by size:
5450 1463 259
Acc65I G'GTAC_C
Cuts at: 4188 4188
Size: 7172
AclI AA'CG_TT
Cuts at: 4151 7012 4151
Size: 2861 4311
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2051 2445 2548 2562 3259 3291 4997 5429 6176
Size: 394 103 14 697 32 1706 432 747
Cuts at: 6176 6444 2051
Size: 268 2779
Fragments arranged by size:
2779 1706 747 697 432 394 268 103
32 14
AfeI AGC'GCT
Cuts at: 427 427
Size: 7172
AflII C'TTAA_G
Cuts at: 1987 1987
Size: 7172
AflIII A'CryG_T
Cuts at: 623 1631 4524 5628 6605 623
Size: 1008 2893 1104 977 1190
Fragments arranged by size:
2893 1190 1104 1008 977
AgeI A'CCGG_T
Cuts at: 1730 5560 1730
Size: 3830 3342
AhdI GACnn_n'nnGTC
Cuts at: 535 1635 535
Size: 1100 6072
AleI CACnn'nnGTG
Cuts at: 154 2884 3709 154
Size: 2730 825 3617
Fragments arranged by size:
3617 2730 825
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 205 811 979 205
Size: 606 168 6398
Fragments arranged by size:
6398 606 168
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 373 405 373
Size: 32 7140
AlwNI CAG_nnn'CTG
Cuts at: 132 135 374 2382 2858 3519 4477 6044 132
Size: 3 239 2008 476 661 958 1567 1260
Fragments arranged by size:
2008 1567 1260 958 661 476 239 3
ApaI G_GGCC'C
Cuts at: 936 3620 936
Size: 2684 4488
ApaLI G'TGCA_C
Cuts at: 1576 2827 3840 5942 1576
Size: 1251 1013 2102 2806
Fragments arranged by size:
2806 2102 1251 1013
ApoI r'AATT_y
Cuts at: 1173 1624 2970 3103 4176 6525 6781 1173
Size: 451 1346 133 1073 2349 256 1564
Fragments arranged by size:
2349 1564 1346 1073 451 256 133
AseI AT'TA_AT
Cuts at: 20 2348 20
Size: 2328 4844
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7172
AvaI C'yCGr_G
Cuts at: 1197 1462 1469 4192 1197
Size: 265 7 2723 4177
Fragments arranged by size:
4177 2723 265 7
AvrII C'CTAG_G
Cuts at: 1915 1915
Size: 7172
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 817 850 817
Size: 33 7139
BamHI G'GATC_C
Cuts at: 4197 4197
Size: 7172
BanI G'GyrC_C
Cuts at: 1585 2038 3006 3640 3951 4188 4602 4850 4885
Size: 453 968 634 311 237 414 248 35
Cuts at: 4885 1585
Size: 3872
Fragments arranged by size:
3872 968 634 453 414 311 248 237
35
BanII G_rGCy'C
Cuts at: 936 1204 1469 2135 3371 3392 3620 4052 4186
Size: 268 265 666 1236 21 228 432 134
Cuts at: 4186 5216 936
Size: 1030 2892
Fragments arranged by size:
2892 1236 1030 666 432 268 265 228
134 21
BbeI G_GCGC'C
Cuts at: 2042 4606 4854 2042
Size: 2564 248 4360
Fragments arranged by size:
4360 2564 248
BbsI GAAGACnn'nnnn_
Cuts at: 1217 1813 2315 2446 2568 3054 3826 1217
Size: 596 502 131 122 486 772 4563
Fragments arranged by size:
4563 772 596 502 486 131 122
BbvCI CC'TCA_GC
Cuts at: 2137 2251 3202 2137
Size: 114 951 6107
Fragments arranged by size:
6107 951 114
BciVI GTATCCnnnnn_n'
Cuts at: 5070 5831 7172 5070
Size: 761 1341 5070
Fragments arranged by size:
5070 1341 761
BclI T'GATC_A
Cuts at: 1514 4692 1514
Size: 3178 3994
BglI GCCn_nnn'nGGC
Cuts at: 2569 3041 2569
Size: 472 6700
BglII A'GATC_T
Cuts at: 2189 4687 6724 2189
Size: 2498 2037 2637
Fragments arranged by size:
2637 2498 2037
BlpI GC'TnA_GC
Cuts at: 952 2851 3230 4284 952
Size: 1899 379 1054 3840
Fragments arranged by size:
3840 1899 1054 379
Bme1580I G_kGCm'C
Cuts at: 936 1580 1588 2831 2929 3620 3844 4797 4890
Size: 644 8 1243 98 691 224 953 93
Cuts at: 4890 5946 936
Size: 1056 2162
Fragments arranged by size:
2162 1243 1056 953 691 644 224 98
93 8
BmgBI CAC'GTC
Cuts at: 1632 2701 3141 1632
Size: 1069 440 5663
Fragments arranged by size:
5663 1069 440
BmrI ACTGGGnnnn_n'
Cuts at: 951 2785 4384 4465 4800 6944 951
Size: 1834 1599 81 335 2144 1179
Fragments arranged by size:
2144 1834 1599 1179 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 381 5539 381
Size: 5158 2014
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 153 351 427 442 2123 2404 2617 2662 2695
Size: 198 76 15 1681 281 213 45 33
Cuts at: 2695 153
Size: 4630
Fragments arranged by size:
4630 1681 281 213 198 76 45 33
15
Bpu10I CC'TnA_GC
Cuts at: 2137 2251 3202 3394 2137
Size: 114 951 192 5915
Fragments arranged by size:
5915 951 192 114
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 749 1024 3774 4348 5227 5719 6017 749
Size: 275 2750 574 879 492 298 1904
Fragments arranged by size:
2750 1904 879 574 492 298 275
BsaI GGTCTCn'nnnn_
Cuts at: 871 1363 1396 2767 3929 4029 6876 871
Size: 492 33 1371 1162 100 2847 1167
Fragments arranged by size:
2847 1371 1167 1162 492 100 33
BsaAI yAC'GTr
Cuts at: 3225 3446 4525 3225
Size: 221 1079 5872
Fragments arranged by size:
5872 1079 221
BsaBI GATnn'nnATC
Cuts at: 6 4686 6723 6
Size: 4680 2037 455
Fragments arranged by size:
4680 2037 455
BsaHI Gr'CG_yC
Cuts at: 293 694 2039 2282 2648 3457 4603 4851 5553
Size: 401 1345 243 366 809 1146 248 702
Cuts at: 5553 7049 293
Size: 1496 416
Fragments arranged by size:
1496 1345 1146 809 702 416 401 366
248 243
BsaWI w'CCGG_w
Cuts at: 455 1730 4361 4882 5560 5834 5981 6392 455
Size: 1275 2631 521 678 274 147 411 1235
Fragments arranged by size:
2631 1275 1235 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 241 271 1393 1423 2004 2034 2535 2565 2590
Size: 30 1122 30 581 30 501 30 25
Cuts at: 2590 2620 3662 3692 241
Size: 30 1042 30 3721
Fragments arranged by size:
3721 1122 1042 581 501 30 30 30
30 30 30 25
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 249 424 1177 1414 1629 2063 2125 2167 3337
Size: 175 753 237 215 434 62 42 1170
Cuts at: 3337 249
Size: 4084
Fragments arranged by size:
4084 1170 753 434 237 215 175 62
42
BseYI C'CCAG_C
Cuts at: 347 443 1674 1767 4597 5355 5932 6428 347
Size: 96 1231 93 2830 758 577 496 1091
Fragments arranged by size:
2830 1231 1091 758 577 496 96 93
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 922 1877 2603 2643 922
Size: 955 726 40 5451
Fragments arranged by size:
5451 955 726 40
BsiEI CG_ry'CG
Cuts at: 104 1380 1389 2810 3629 4714 4760 5968 104
Size: 1276 9 1421 819 1085 46 1208 1308
Fragments arranged by size:
1421 1308 1276 1208 1085 819 46 9
BsiHKAI G_wGCw'C
Cuts at: 800 1033 1580 2055 2831 3371 3392 3509 3844
Size: 233 547 475 776 540 21 117 335
Cuts at: 3844 4186 4964 5154 5946 800
Size: 342 778 190 792 2026
Fragments arranged by size:
2026 792 778 776 547 540 475 342
335 233 190 117 21
BsmBI CGTCTCn'nnnn_
Cuts at: 2922 3131 2922
Size: 209 6963
Bsp1286I G_dGCh'C
Cuts at: 800 936 1033 1204 1469 1580 1588 2055 2135
Size: 136 97 171 265 111 8 467 80
Cuts at: 2135 2831 2929 3371 3392 3509 3620 3844 4052
Size: 696 98 442 21 117 111 224 208
Cuts at: 4052 4186 4797 4890 4964 5154 5216 5946 800
Size: 134 611 93 74 190 62 730 2026
Fragments arranged by size:
2026 730 696 611 467 442 265 224
208 190 171 136 134 117 111 111
98 97 93 80 74 62 21 8
BspEI T'CCGG_A
Cuts at: 6392 6392
Size: 7172
BspHI T'CATG_A
Cuts at: 6348 6348
Size: 7172
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2002 2527 3518 4222 4436 4738 5119 2002
Size: 525 991 704 214 302 381 4055
Fragments arranged by size:
4055 991 704 525 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5464 5518 7002 7167 50
Size: 5414 54 1484 165 55
Fragments arranged by size:
5414 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 5084 5084
Size: 7172
BsrFI r'CCGG_y
Cuts at: 320 449 1684 1730 2035 2686 3706 5170 5351
Size: 129 1235 46 305 651 1020 1464 181
Cuts at: 5351 5560 320
Size: 209 1932
Fragments arranged by size:
1932 1464 1235 1020 651 305 209 181
129 46
BssSI C'ACGA_G
Cuts at: 5443 5801 5443
Size: 358 6814
BstAPI GCAn_nnn'nTGC
Cuts at: 135 3347 4549 135
Size: 3212 1202 2758
Fragments arranged by size:
3212 2758 1202
BstBI TT'CG_AA
Cuts at: 5533 5533
Size: 7172
BstXI CCAn_nnnn'nTGG
Cuts at: 1665 2628 2886 3450 3696 3711 3907 1665
Size: 963 258 564 246 15 196 4930
Fragments arranged by size:
4930 963 564 258 246 196 15
BstYI r'GATC_y
Cuts at: 1 523 2189 3150 3167 3515 4197 4664 4687
Size: 522 1666 961 17 348 682 467 23
Cuts at: 4687 5022 5268 6280 6366 6378 6724 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1666 1012 961 682 522 467 449 348
346 335 246 86 23 17 12
Bsu36I CC'TnA_GG
Cuts at: 1908 1908
Size: 7172
BtgI C'CryG_G
Cuts at: 107 969 3132 3612 4039 107
Size: 862 2163 480 427 3240
Fragments arranged by size:
3240 2163 862 480 427
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3669 5122 5303 5466 3669
Size: 1453 181 163 5375
Fragments arranged by size:
5375 1453 181 163
BtsI GCAGTG_nn'
Cuts at: 240 2703 4415 240
Size: 2463 1712 2997
Fragments arranged by size:
2997 2463 1712
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 4145 4180 4145
Size: 35 7137
DraI TTT'AAA
Cuts at: 1623 4171 1623
Size: 2548 4624
DraIII CAC_nnn'GTG
Cuts at: 2292 2292
Size: 7172
DrdI GACnn_nn'nnGTC
Cuts at: 3895 4878 5736 3895
Size: 983 858 5331
Fragments arranged by size:
5331 983 858
EaeI y'GGCC_r
Cuts at: 731 1655 1682 2540 2741 2807 3042 3626 4757
Size: 924 27 858 201 66 235 584 1131
Cuts at: 4757 4931 5322 5349 6694 731
Size: 174 391 27 1345 1209
Fragments arranged by size:
1345 1209 1131 924 858 584 391 235
201 174 66 27 27
EagI C'GGCC_G
Cuts at: 2807 3626 4757 2807
Size: 819 1131 5222
Fragments arranged by size:
5222 1131 819
EarI CTCTTCn'nnn_
Cuts at: 472 2408 3731 5195 5405 472
Size: 1936 1323 1464 210 2239
Fragments arranged by size:
2239 1936 1464 1323 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 329 1243 5380 5690 5836 6982 329
Size: 914 4137 310 146 1146 519
Fragments arranged by size:
4137 1146 914 519 310 146
EcoICRI GAG'CTC
Cuts at: 3369 3390 4184 3369
Size: 21 794 6357
Fragments arranged by size:
6357 794 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 153 351 427 442 2051 2123 2404 2445 2548
Size: 198 76 15 1609 72 281 41 103
Cuts at: 2548 2562 2617 2662 2695 3259 3291 4997 5429
Size: 14 55 45 33 564 32 1706 432
Cuts at: 5429 6176 6444 153
Size: 747 268 881
Fragments arranged by size:
1706 1609 881 747 564 432 281 268
198 103 76 72 55 45 41 33
32 15 14
EcoNI CCTnn'n_nnAGG
Cuts at: 409 701 1396 2374 2862 409
Size: 292 695 978 488 4719
Fragments arranged by size:
4719 978 695 488 292
EcoO109I rG'GnC_Cy
Cuts at: 362 381 932 933 1071 1912 2107 2867 3357
Size: 19 551 1 138 841 195 760 490
Cuts at: 3357 3617 4311 362
Size: 260 694 3223
Fragments arranged by size:
3223 841 760 694 551 490 260 195
138 19 1
EcoRI G'AATT_C
Cuts at: 1173 4176 1173
Size: 3003 4169
EcoRV GAT'ATC
Cuts at: 1305 1820 1305
Size: 515 6657
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3169 5191 3169
Size: 2022 5150
FspI TGC'GCA
Cuts at: 328 677 4953 328
Size: 349 4276 2547
Fragments arranged by size:
4276 2547 349
HaeII r_GCGC'y
Cuts at: 429 450 2042 2670 4606 4854 5876 6855 429
Size: 21 1592 628 1936 248 1022 979 746
Fragments arranged by size:
1936 1592 1022 979 746 628 248 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 521 553 831 863 1621 1653 1809 1841 2391
Size: 32 278 32 758 32 156 32 550
Cuts at: 2391 2423 2590 2622 3269 3301 3662 3694 3964
Size: 32 167 32 647 32 361 32 270
Cuts at: 3964 3996 5014 5044 5046 5076 521
Size: 32 1018 30 2 30 2617
Fragments arranged by size:
2617 1018 758 647 550 361 278 270
167 156 32 32 32 32 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2489 3303 3681 4211 2489
Size: 814 378 530 5450
Fragments arranged by size:
5450 814 530 378
KasI G'GCGC_C
Cuts at: 2038 4602 4850 2038
Size: 2564 248 4360
Fragments arranged by size:
4360 2564 248
KpnI G_GTAC'C
Cuts at: 4192 4192
Size: 7172
MluI A'CGCG_T
Cuts at: 623 6605 623
Size: 5982 1190
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 248 746 1664 5843 6027 248
Size: 498 918 4179 184 1393
Fragments arranged by size:
4179 1393 918 498 184
MscI TGG'CCA
Cuts at: 1657 4933 1657
Size: 3276 3896
MslI CAynn'nnrTG
Cuts at: 154 793 1321 1498 2626 2722 2884 3448 3709
Size: 639 528 177 1128 96 162 564 261
Cuts at: 3709 3727 5161 5288 154
Size: 18 1434 127 2038
Fragments arranged by size:
2038 1434 1128 639 564 528 261 177
162 127 96 18
MspA1I CmG'CkG
Cuts at: 132 173 216 351 374 1674 1853 2250 2901
Size: 41 43 135 23 1300 179 397 651
Cuts at: 2901 3237 3275 3873 4283 4597 4957 5970 6215
Size: 336 38 598 410 314 360 1013 245
Cuts at: 6215 132
Size: 1089
Fragments arranged by size:
1300 1089 1013 651 598 410 397 360
336 314 245 179 135 43 41 38
23
NaeI GCC'GGC
Cuts at: 322 1686 2037 2688 5353 322
Size: 1364 351 651 2665 2141
Fragments arranged by size:
2665 2141 1364 651 351
NarI GG'CG_CC
Cuts at: 2039 4603 4851 2039
Size: 2564 248 4360
Fragments arranged by size:
4360 2564 248
NcoI C'CATG_G
Cuts at: 107 3612 107
Size: 3505 3667
NgoMIV G'CCGG_C
Cuts at: 320 1684 2035 2686 5351 320
Size: 1364 351 651 2665 2141
Fragments arranged by size:
2665 2141 1364 651 351
NruI TCG'CGA
Cuts at: 3101 3101
Size: 7172
NspI r_CATG'y
Cuts at: 1192 3180 3576 4225 4725 5632 1192
Size: 1988 396 649 500 907 2732
Fragments arranged by size:
2732 1988 907 649 500 396
PasI CC'CwG_GG
Cuts at: 1428 1428
Size: 7172
PciI A'CATG_T
Cuts at: 5628 5628
Size: 7172
PflMI CCAn_nnn'nTGG
Cuts at: 374 1350 1779 2292 3051 3969 374
Size: 976 429 513 759 918 3577
Fragments arranged by size:
3577 976 918 759 513 429
PfoI T'CCnGG_A
Cuts at: 896 1024 2267 2369 2471 2639 2857 896
Size: 128 1243 102 102 168 218 5211
Fragments arranged by size:
5211 1243 218 168 128 102 102
PmeI GTTT'AAAC
Cuts at: 4171 4171
Size: 7172
PmlI CAC'GTG
Cuts at: 3225 3225
Size: 7172
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6311 6343 6311
Size: 32 7140
PpuMI rG'GwC_Cy
Cuts at: 381 1071 2107 2867 3357 381
Size: 690 1036 760 490 4196
Fragments arranged by size:
4196 1036 760 690 490
PshAI GACnn'nnGTC
Cuts at: 697 697
Size: 7172
PspOMI G'GGCC_C
Cuts at: 932 3616 932
Size: 2684 4488
PspXI vC'TCGA_Gb
Cuts at: 1197 1197
Size: 7172
PstI C_TGCA'G
Cuts at: 271 1852 3515 4219 4904 271
Size: 1581 1663 704 685 2539
Fragments arranged by size:
2539 1663 1581 704 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7172
PvuII CAG'CTG
Cuts at: 132 216 351 1674 1853 3237 3873 4597 4957
Size: 84 135 1323 179 1384 636 724 360
Cuts at: 4957 132
Size: 2347
Fragments arranged by size:
2347 1384 1323 724 636 360 179 135
84
RsrII CG'GwC_CG
Cuts at: 5367 5367
Size: 7172
SacI G_AGCT'C
Cuts at: 3371 3392 4186 3371
Size: 21 794 6357
Fragments arranged by size:
6357 794 21
SalI G'TCGA_C
Cuts at: 2487 4209 2487
Size: 1722 5450
SanDI GG'GwC_CC
Cuts at: 381 381
Size: 7172
SapI GCTCTTCn'nnn_
Cuts at: 472 5195 5405 472
Size: 4723 210 2239
Fragments arranged by size:
4723 2239 210
SbfI CC_TGCA'GG
Cuts at: 4219 4219
Size: 7172
ScaI AGT'ACT
Cuts at: 6689 6689
Size: 7172
SexAI A'CCwGG_T
Cuts at: 3947 3947
Size: 7172
SfcI C'TryA_G
Cuts at: 33 267 1244 1848 2394 3408 3511 3780 4215
Size: 234 977 604 546 1014 103 269 435
Cuts at: 4215 4900 5893 6084 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 1014 993 977 685 604 546 435
269 234 191 103
SfoI GGC'GCC
Cuts at: 2040 4604 4852 2040
Size: 2564 248 4360
Fragments arranged by size:
4360 2564 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7172
SgrAI Cr'CCGG_yG
Cuts at: 3706 3706
Size: 7172
SmaI CCC'GGG
Cuts at: 1471 4194 1471
Size: 2723 4449
SmlI C'TyrA_G
Cuts at: 764 1039 1197 1987 3753 4327 5242 5734 5996
Size: 275 158 790 1766 574 915 492 262
Cuts at: 5996 764
Size: 1940
Fragments arranged by size:
1940 1766 915 790 574 492 275 262
158
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7172
SphI G_CATG'C
Cuts at: 1192 3180 4225 4725 1192
Size: 1988 1045 500 3639
Fragments arranged by size:
3639 1988 1045 500
StuI AGG'CCT
Cuts at: 415 1864 415
Size: 1449 5723
StyI C'CwwG_G
Cuts at: 107 1835 1915 2093 3533 3612 4010 4306 4660
Size: 1728 80 178 1440 79 398 296 354
Cuts at: 4660 107
Size: 2619
Fragments arranged by size:
2619 1728 1440 398 354 296 178 80
79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1366 4755 4888 5347 5556 1366
Size: 3389 133 459 209 2982
Fragments arranged by size:
3389 2982 459 209 133
TatI w'GTAC_w
Cuts at: 2964 6687 2964
Size: 3723 3449
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 508 540 1393 1425 1935 1967 2004 2036 2535
Size: 32 853 32 510 32 37 32 499
Cuts at: 2535 2567 3859 3891 6689 6721 508
Size: 32 1292 32 2798 32 959
Fragments arranged by size:
2798 1292 959 853 510 499 37 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1932 2508 3676 4969 1932
Size: 576 1168 1293 4135
Fragments arranged by size:
4135 1293 1168 576
XbaI T'CTAG_A
Cuts at: 4203 4203
Size: 7172
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2670 4667 2670
Size: 1997 5175
XhoI C'TCGA_G
Cuts at: 1197 1197
Size: 7172
XmaI C'CCGG_G
Cuts at: 1469 4192 1469
Size: 2723 4449
ZraI GAC'GTC
Cuts at: 2649 2649
Size: 7172
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NruI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AscI BcgI BfrBI BmtI BsiWI BsmI BsrGI BssHII
BstEII BstZ17I ClaI FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NsiI PacI PsiI PsrI SacII
SfiI SnaBI SrfI SspI SwaI XmnI