################################## # NO CUT ENZYMES ( 177 enzymes) # ################################## AatII AccI AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AspHI AspI AviII AvrII BalI BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsiHKAI BsiWI BsmAI BsmBI BsmI Bsp1286I BspDI BspHI BspLU11I BspMI BsrBI BsrDI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstYI BstZ17I Bsu36I CelII CfoI ClaI CpoI DdeI DpnII DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FokI FseI FspI HaeII HaeIII HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KspI MaeII MamI MboI MfeI MflI MluI MluNI MscI MslI MunI NaeI NarI NciI NcoI NdeII NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I StuI StyI SwaI TaqI Tth111I TthHB8I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### Acc65I 122 GGTACC G^GTACC AcsI 456 AAATTC [GA]^AATT[CT] AlwNI 296 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 456 AAATTC [GA]^AATT[CT] Asp718I 122 GGTACC G^GTACC AvaI 163 CCCGAG C^[CT]CG[GA]G AvaII 29 GGACC G^G[AT]CC BanI 122 GGTACC G^G[CT][GA]CC BsgI 506 CTGCAC GTGCAG(16/14) BsiEI 36 CGACCG CG[GA][CT]^CG BsoBI 163 CCCGAG C^[CT]CG[GA]G Bsp1407I 402 TGTACA T^GTACA BsrFI 119 GCCGGT [GA]^CCGG[CT] BsrGI 402 TGTACA T^GTACA BstXI 127 CCAGACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 119 GCCGGT [GA]^CCGG[CT] Cfr13I 29 GGACC G^G[ACGT]CC EarI 345 GAAGAG CTCTTC(1/4) FauI 57 GCGGG CCCGC(4/6) HgaI 24 GACGC GACGC(5/10) HphI 108 GGTGA GGTGA(8/7) KpnI 122 GGTACC GGTAC^C Ksp632I 345 GAAGAG CTCTTC(1/4) MseI 332 TTAA T^TAA MspA1I 503 CCGCTG C[AC]G^C[GT]G MwoI 74 GCTTCCGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NdeI 278 CATATG CA^TATG NlaIII 406 CATG CATG^ NspI 405 ACATGC [GA]CATG^[CT] PflMI 445 CCAGCCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 215 GAGTC GAGTC(4/5) Sau96I 29 GGACC G^G[ACGT]CC SfaNI 370 GATGC GCATC(5/9) SfcI 461 CTACAG C^T[GA][CT]AG SspBI 402 TGTACA T^GTACA TfiI 325 GATTC G^A[AT]TC Tru9I 332 TTAA T^TAA Tsp45I 298 GTCAC ^GT[GC]AC Tsp509I 457 AATT ^AATT Van91I 445 CCAGCCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccII 26 CGCG CG^CG 40 CGCG AccIII 77 TCCGGA T^CCGGA 477 TCCGGA AfaI 123 GTAC GT^AC 403 GTAC AluI 425 AGCT AG^CT 431 AGCT BfaI 229 CTAG C^TAG 530 CTAG BpmI 385 CTCCAG CTGGAG(16/14) 212 CTGGAG BsaJI 144 CCCAGG C^C[ACGT][ACGT]GG 164 CCGAGG BsaWI 77 TCCGGA [AT]^CCGG[AT] 477 TCCGGA BseAI 77 TCCGGA T^CCGGA 477 TCCGGA BsiYI 145 CCAGGAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 445 CCAGCCAATGG BslI 145 CCAGGAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 445 CCAGCCAATGG BsmFI 142 GTCCC GGGAC(10/14) 466 GTCCC BspEI 77 TCCGGA T^CCGGA 477 TCCGGA BsrI 132 CCAGT ACTGG(1/-1) 387 CCAGT BssKI 145 CCAGG ^CC[ACGT]GG 175 CCTGG BstNI 145 CCAGG CC^[AT]GG 175 CCTGG BstUI 26 CGCG CG^CG 40 CGCG EcoRII 145 CCAGG ^CC[AT]GG 175 CCTGG HinfI 215 GAGTC G^A[ACGT]TC 325 GATTC MaeI 229 CTAG C^TAG 530 CTAG MaeIII 298 GTCAC ^GT[ACGT]AC 499 GTAAC MroI 77 TCCGGA T^CCGGA 477 TCCGGA MvaI 145 CCAGG CC^[AT]GG 175 CCTGG MvnI 26 CGCG CG^CG 40 CGCG RsaI 123 GTAC GT^AC 403 GTAC ScrFI 145 CCAGG CC^[ACGT]GG 175 CCTGG SspI 306 AATATT AAT^ATT 334 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 14 enzymes) # ######################################### AciI BbvI BseRI Cac8I Fnu4HI HapII HpaII ItaI MboII MnlI MspI NlaIV TseI TspRI