Restriction map for
pF1KA0487
(Circular) (Six-base) MAPSORT of: pF1KA0487.seq Check: 2892 from: 1 to: 3653
pF1KA0487 3653 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 691 691
Size: 3653
Acc65I G'GTAC_C
Cuts at: 230 669 230
Size: 439 3214
AclI AA'CG_TT
Cuts at: 3493 3493
Size: 3653
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1478 1910 2657 2925 1478
Size: 432 747 268 2206
Fragments arranged by size:
2206 747 432 268
AflIII A'CryG_T
Cuts at: 1005 2109 3086 1005
Size: 1104 977 1572
Fragments arranged by size:
1572 1104 977
AgeI A'CCGG_T
Cuts at: 2041 2041
Size: 3653
AlwNI CAG_nnn'CTG
Cuts at: 409 958 2525 409
Size: 549 1567 1537
Fragments arranged by size:
1567 1537 549
ApaLI G'TGCA_C
Cuts at: 2423 2423
Size: 3653
ApoI r'AATT_y
Cuts at: 564 657 3006 3262 564
Size: 93 2349 256 955
Fragments arranged by size:
2349 955 256 93
AseI AT'TA_AT
Cuts at: 20 20
Size: 3653
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3653
AvaI C'yCGr_G
Cuts at: 271 673 271
Size: 402 3251
BamHI G'GATC_C
Cuts at: 678 678
Size: 3653
BanI G'GyrC_C
Cuts at: 230 669 1083 1331 1366 230
Size: 439 414 248 35 2517
Fragments arranged by size:
2517 439 414 248 35
BanII G_rGCy'C
Cuts at: 667 1697 667
Size: 1030 2623
BbeI G_GCGC'C
Cuts at: 1087 1335 1087
Size: 248 3405
BciVI GTATCCnnnnn_n'
Cuts at: 1551 2312 3653 1551
Size: 761 1341 1551
Fragments arranged by size:
1551 1341 761
BclI T'GATC_A
Cuts at: 1173 1173
Size: 3653
BglII A'GATC_T
Cuts at: 1168 3205 1168
Size: 2037 1616
BlpI GC'TnA_GC
Cuts at: 765 765
Size: 3653
Bme1580I G_kGCm'C
Cuts at: 1278 1371 2427 1278
Size: 93 1056 2504
Fragments arranged by size:
2504 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 865 946 1281 3425 865
Size: 81 335 2144 1093
Fragments arranged by size:
2144 1093 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2020 2020
Size: 3653
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 341 478 341
Size: 137 3516
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 829 1708 2200 2498 829
Size: 879 492 298 1984
Fragments arranged by size:
1984 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3357 3357
Size: 3653
BsaAI yAC'GTr
Cuts at: 1006 1006
Size: 3653
BsaBI GATnn'nnATC
Cuts at: 6 1167 3204 6
Size: 1161 2037 455
Fragments arranged by size:
2037 1161 455
BsaHI Gr'CG_yC
Cuts at: 1084 1332 2034 3530 1084
Size: 248 702 1496 1207
Fragments arranged by size:
1496 1207 702 248
BsaWI w'CCGG_w
Cuts at: 185 585 842 1363 2041 2315 2462 2873 185
Size: 400 257 521 678 274 147 411 965
Fragments arranged by size:
965 678 521 411 400 274 257 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 272 289 328 272
Size: 17 39 3597
Fragments arranged by size:
3597 39 17
BseYI C'CCAG_C
Cuts at: 540 552 1078 1836 2413 2909 540
Size: 12 526 758 577 496 1284
Fragments arranged by size:
1284 758 577 526 496 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 599 599
Size: 3653
BsiEI CG_ry'CG
Cuts at: 104 147 1195 1241 2449 104
Size: 43 1048 46 1208 1308
Fragments arranged by size:
1308 1208 1048 46 43
BsiHKAI G_wGCw'C
Cuts at: 667 1445 1635 2427 667
Size: 778 190 792 1893
Fragments arranged by size:
1893 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 667 1278 1371 1445 1635 1697 2427 667
Size: 611 93 74 190 62 730 1893
Fragments arranged by size:
1893 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 185 585 2873 185
Size: 400 2288 965
Fragments arranged by size:
2288 965 400
BspHI T'CATG_A
Cuts at: 2829 2829
Size: 3653
BspMI ACCTGCnnnn'nnnn_
Cuts at: 703 917 1219 1600 703
Size: 214 302 381 2756
Fragments arranged by size:
2756 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1945 1999 3483 3648 50
Size: 1895 54 1484 165 55
Fragments arranged by size:
1895 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1565 1565
Size: 3653
BsrFI r'CCGG_y
Cuts at: 227 1651 1832 2041 227
Size: 1424 181 209 1839
Fragments arranged by size:
1839 1424 209 181
BsrGI T'GTAC_A
Cuts at: 510 510
Size: 3653
BssSI C'ACGA_G
Cuts at: 1924 2282 1924
Size: 358 3295
BstAPI GCAn_nnn'nTGC
Cuts at: 1030 1030
Size: 3653
BstBI TT'CG_AA
Cuts at: 2014 2014
Size: 3653
BstXI CCAn_nnnn'nTGG
Cuts at: 242 242
Size: 3653
BstYI r'GATC_y
Cuts at: 1 678 1145 1168 1503 1749 2761 2847 2859
Size: 677 467 23 335 246 1012 86 12
Cuts at: 2859 3205 1
Size: 346 449
Fragments arranged by size:
1012 677 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1603 1784 1947 1603
Size: 181 163 3309
Fragments arranged by size:
3309 181 163
BtsI GCAGTG_nn'
Cuts at: 896 896
Size: 3653
DraI TTT'AAA
Cuts at: 652 652
Size: 3653
DrdI GACnn_nn'nnGTC
Cuts at: 1359 2217 1359
Size: 858 2795
EaeI y'GGCC_r
Cuts at: 1238 1412 1803 1830 3175 1238
Size: 174 391 27 1345 1716
Fragments arranged by size:
1716 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1238 1238
Size: 3653
EarI CTCTTCn'nnn_
Cuts at: 448 1676 1886 448
Size: 1228 210 2215
Fragments arranged by size:
2215 1228 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1861 2171 2317 3463 1861
Size: 310 146 1146 2051
Fragments arranged by size:
2051 1146 310 146
EcoICRI GAG'CTC
Cuts at: 665 665
Size: 3653
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 341 478 1478 1910 2657 2925 341
Size: 137 1000 432 747 268 1069
Fragments arranged by size:
1069 1000 747 432 268 137
EcoO109I rG'GnC_Cy
Cuts at: 792 792
Size: 3653
EcoRI G'AATT_C
Cuts at: 657 657
Size: 3653
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1672 1672
Size: 3653
FspI TGC'GCA
Cuts at: 1434 1434
Size: 3653
HaeII r_GCGC'y
Cuts at: 1087 1335 2357 3336 1087
Size: 248 1022 979 1404
Fragments arranged by size:
1404 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1495 1525 1527 1557 1495
Size: 30 2 30 3591
Fragments arranged by size:
3591 30 30 2
HincII GTy'rAC
Cuts at: 692 692
Size: 3653
KasI G'GCGC_C
Cuts at: 1083 1331 1083
Size: 248 3405
KpnI G_GTAC'C
Cuts at: 234 673 234
Size: 439 3214
MluI A'CGCG_T
Cuts at: 3086 3086
Size: 3653
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2324 2508 2324
Size: 184 3469
MscI TGG'CCA
Cuts at: 1414 1414
Size: 3653
MslI CAynn'nnrTG
Cuts at: 1642 1769 1642
Size: 127 3526
MspA1I CmG'CkG
Cuts at: 613 764 1078 1438 2451 2696 613
Size: 151 314 360 1013 245 1570
Fragments arranged by size:
1570 1013 360 314 245 151
NaeI GCC'GGC
Cuts at: 1834 1834
Size: 3653
NarI GG'CG_CC
Cuts at: 1084 1332 1084
Size: 248 3405
NdeI CA'TA_TG
Cuts at: 387 387
Size: 3653
NgoMIV G'CCGG_C
Cuts at: 1832 1832
Size: 3653
NspI r_CATG'y
Cuts at: 517 706 1206 2113 517
Size: 189 500 907 2057
Fragments arranged by size:
2057 907 500 189
PciI A'CATG_T
Cuts at: 2109 2109
Size: 3653
PflMI CCAn_nnn'nTGG
Cuts at: 559 559
Size: 3653
PmeI GTTT'AAAC
Cuts at: 652 652
Size: 3653
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2792 2824 2792
Size: 32 3621
PstI C_TGCA'G
Cuts at: 700 1385 700
Size: 685 2968
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3653
PvuII CAG'CTG
Cuts at: 1078 1438 1078
Size: 360 3293
RsrII CG'GwC_CG
Cuts at: 1848 1848
Size: 3653
SacI G_AGCT'C
Cuts at: 667 667
Size: 3653
SalI G'TCGA_C
Cuts at: 690 690
Size: 3653
SapI GCTCTTCn'nnn_
Cuts at: 1676 1886 1676
Size: 210 3443
SbfI CC_TGCA'GG
Cuts at: 700 700
Size: 3653
ScaI AGT'ACT
Cuts at: 3170 3170
Size: 3653
SfcI C'TryA_G
Cuts at: 33 569 696 1381 2374 2565 33
Size: 536 127 685 993 191 1121
Fragments arranged by size:
1121 993 685 536 191 127
SfoI GGC'GCC
Cuts at: 1085 1333 1085
Size: 248 3405
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3653
SmaI CCC'GGG
Cuts at: 675 675
Size: 3653
SmlI C'TyrA_G
Cuts at: 808 1723 2215 2477 808
Size: 915 492 262 1984
Fragments arranged by size:
1984 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3653
SphI G_CATG'C
Cuts at: 706 1206 706
Size: 500 3153
SspI AAT'ATT
Cuts at: 416 444 416
Size: 28 3625
StyI C'CwwG_G
Cuts at: 787 1141 787
Size: 354 3299
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 386 1236 1369 1828 2037 386
Size: 850 133 459 209 2002
Fragments arranged by size:
2002 850 459 209 133
TatI w'GTAC_w
Cuts at: 510 3168 510
Size: 2658 995
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3170 3202 3170
Size: 32 3621
Tth111I GACn'n_nGTC
Cuts at: 1450 1450
Size: 3653
XbaI T'CTAG_A
Cuts at: 684 684
Size: 3653
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1148 1148
Size: 3653
XmaI C'CCGG_G
Cuts at: 673 673
Size: 3653
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NdeI NgoMIV NspI PciI PflMI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI Bpu10I BsaXI BsiWI BsmI BsmBI
BssHII BstEII BstZ17I Bsu36I BtgI ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NheI NotI NruI NsiI PacI PasI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
XmnI ZraI