Restriction map for pF1KA0487
 (Linear) (Six-base) MAPSORT of: KIAA0487.seq  Check: 5834  from: 1  to: 540



KIAA0487.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:35  ..

Acc65I G'GTAC_C

Cuts at:      0     122     540 
   Size:        122     418
 
AlwNI CAG_nnn'CTG

Cuts at:      0     301     540 
   Size:        301     239
 
ApoI r'AATT_y

Cuts at:      0     456     540 
   Size:        456      84
 
AvaI C'yCGr_G

Cuts at:      0     163     540 
   Size:        163     377
 
BanI G'GyrC_C

Cuts at:      0     122     540 
   Size:        122     418
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     233     370     540 
   Size:        233     137     170

  Fragments arranged by size: 

                 233     170     137
 
BsaWI w'CCGG_w

Cuts at:      0      77     477     540 
   Size:         77     400      63

  Fragments arranged by size: 

                 400      77      63
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     164     181     220     540 
   Size:        164      17      39     320

  Fragments arranged by size: 

                 320     164      39      17
 
BseYI C'CCAG_C

Cuts at:      0     432     444     540 
   Size:        432      12      96

  Fragments arranged by size: 

                 432      96      12
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     491     540 
   Size:        491      49
 
BsiEI CG_ry'CG

Cuts at:      0      39     540 
   Size:         39     501
 
BspEI T'CCGG_A

Cuts at:      0      77     477     540 
   Size:         77     400      63

  Fragments arranged by size: 

                 400      77      63
 
BsrFI r'CCGG_y

Cuts at:      0     119     540 
   Size:        119     421
 
BsrGI T'GTAC_A

Cuts at:      0     402     540 
   Size:        402     138
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     134     540 
   Size:        134     406
 
EarI CTCTTCn'nnn_

Cuts at:      0     340     540 
   Size:        340     200
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     233     370     540 
   Size:        233     137     170

  Fragments arranged by size: 

                 233     170     137
 
KpnI G_GTAC'C

Cuts at:      0     126     540 
   Size:        126     414
 
MspA1I CmG'CkG

Cuts at:      0     505     540 
   Size:        505      35
 
NdeI CA'TA_TG

Cuts at:      0     279     540 
   Size:        279     261
 
NspI r_CATG'y

Cuts at:      0     409     540 
   Size:        409     131
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     451     540 
   Size:        451      89
 
SfcI C'TryA_G

Cuts at:      0     461     540 
   Size:        461      79
 
SspI AAT'ATT

Cuts at:      0     308     336     540 
   Size:        308      28     204

  Fragments arranged by size: 

                 308     204      28
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     278     540 
   Size:        278     262
 
TatI w'GTAC_w

Cuts at:      0     402     540 
   Size:        402     138
 

 Enzymes that do cut:

   Acc65I    AlwNI     ApoI     AvaI     BanI     BpmI    BsaWI    BseRI
    BseYI     BsgI    BsiEI    BspEI    BsrFI    BsrGI    BstXI     EarI
  Eco57MI     KpnI   MspA1I     NdeI     NspI    PflMI     SfcI     SspI
    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaXI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspHI    BspMI    BsrBI    BsrDI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     MfeI
     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI
     StyI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI
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