################################## # NO CUT ENZYMES ( 54 enzymes) # ################################## AatII Acc65I AclI AflII AflIII AgeI Aor51HI AscI AseI AsnI Asp718I AspI AvrII BclI BfrI BlnI BsiWI BspDI BspLU11I BstBI ClaI CpoI DraI Eco47III EcoRV EcoT22I FbaI HpaI KpnI MfeI MluI MunI NdeI NheI NotI NruI NsiI NspV PacI PflFI PinAI PmeI PshBI Psp1406I PvuI RsrII SalI SfuI SgrAI SpeI SphI SspI SwaI Tth111I ################################### # ONE CUT ENZYMES ( 28 enzymes) # ################################### AccIII 6235 TCCGGA T^CCGGA BamHI 3480 GGATCC G^GATCC BsaWI 6235 TCCGGA [AT]^CCGG[AT] BseAI 6235 TCCGGA T^CCGGA BsiEI 3012 CGGCCG CG[GA][CT]^CG BspEI 6235 TCCGGA T^CCGGA BssHII 5727 GCGCGC G^CGCGC BstEII 3858 GGTGACC G^GT[ACGT]ACC BstPI 3858 GGTGACC G^GT[ACGT]ACC EagI 3012 CGGCCG C^GGCCG EclXI 3012 CGGCCG C^GGCCG Eco52I 3012 CGGCCG C^GGCCG EcoO65I 3858 GGTGACC G^GT[ACGT]ACC EcoRI 4627 GAATTC G^AATTC HincII 3985 GTCAAC GT[CT]^[GA]AC HindII 3985 GTCAAC GT[CT]^[GA]AC HindIII 4942 AAGCTT A^AGCTT KspI 3510 CCGCGG CCGC^GG MroI 6235 TCCGGA T^CCGGA MseI 5141 TTAA T^TAA PshAI 3861 GACCGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 3510 CCGCGG CCGC^GG SbfI 708 CCTGCAGG CCTGCA^GG SfiI 398 GGCCAGAGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SnaBI 1813 TACGTA TAC^GTA Sse8387I 708 CCTGCAGG CCTGCA^GG Tru9I 5141 TTAA T^TAA XbaI 721 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcsI 4060 GAATTT [GA]^AATT[CT] 4627 GAATTC AhdI 3446 GACGTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 6170 GACAGACTGTC ApoI 4060 GAATTT [GA]^AATT[CT] 4627 GAATTC Asp700I 4623 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5853 GAAGTACTTC AspEI 3446 GACGTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 6170 GACAGACTGTC AviII 1914 TGCGCA TGC^GCA 5027 TGCGCA BbsI 4281 GAAGAC GAAGAC(2/6) 5928 GAAGAC BciVI 511 GGATAC GTATCC(6/5) 1816 GTATCC BglII 5747 AGATCT A^GATCT 6399 AGATCT BpuAI 4281 GAAGAC GAAGAC(2/6) 5928 GAAGAC BsaBI 1117 GATGCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 5070 GATGTCCATC Bsp1407I 4529 TGTACA T^GTACA 5120 TGTACA BspHI 1403 TCATGA T^CATGA 4640 TCATGA BsrDI 6033 CATTGC GCAATG(2/0) 4228 GCAATG BsrGI 4529 TGTACA T^GTACA 5120 TGTACA Bst1107I 3741 GTATAC GTA^TAC 5085 GTATAC BstZ17I 3741 GTATAC GTA^TAC 5085 GTATAC Eam1105I 3446 GACGTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 6170 GACAGACTGTC FseI 916 GGCCGGCC GGCCGG^CC 3009 GGCCGGCC FspI 1914 TGCGCA TGC^GCA 5027 TGCGCA MamI 1117 GATGCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 5070 GATGTCCATC RcaI 1403 TCATGA T^CATGA 4640 TCATGA SacI 1831 GAGCTC GAGCT^C 2980 GAGCTC ScaI 5855 AGTACT AGT^ACT 6290 AGTACT SspBI 4529 TGTACA T^GTACA 5120 TGTACA XmnI 4623 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5853 GAAGTACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 150 enzymes) # ######################################### AccI AccII AciI AcyI AfaI AluI Alw44I AlwI AlwNI AocI ApaI ApaLI AspHI AvaI AvaII BalI BanI BanII BbeI BbrPI BbvCI BbvI BcgI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DrdI DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PaeR7I PflMI PleI PmaCI PmlI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SfoI SmaI SmlI StuI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoI XhoII XmaCI XmaI