Restriction map for pF1KA0335
 (Linear) (Six-base) MAPSORT of: KIAA0335.seq  Check: 6278  from: 1  to: 4449



KIAA0335.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:35  ..

Acc65I G'GTAC_C

Cuts at:      0    1774    4449 
   Size:       1774    2675
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     335     730    1094    1232    2344    2363    3428    3935 
   Size:        335     395     364     138    1112      19    1065     507

Cuts at:   3935    4449 
   Size:        514

  Fragments arranged by size: 

                1112    1065     514     507     395     364     335     138
                  19
 
AflII C'TTAA_G

Cuts at:      0     931    1042    2662    3352    4123    4449 
   Size:        931     111    1620     690     771     326

  Fragments arranged by size: 

                1620     931     771     690     326     111
 
AflIII A'CryG_T

Cuts at:      0     837    4116    4439    4449 
   Size:        837    3279     323      10

  Fragments arranged by size: 

                3279     837     323      10
 
AleI CACnn'nnGTG

Cuts at:      0    1036    4449 
   Size:       1036    3413
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1560    1592    4449 
   Size:       1560      32    2857

  Fragments arranged by size: 

                2857    1560      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1426    3677    3827    4449 
   Size:       1426    2251     150     622

  Fragments arranged by size: 

                2251    1426     622     150
 
ApoI r'AATT_y

Cuts at:      0     107     692    1692    1705    2217    2329    2565    2572 
   Size:        107     585    1000      13     512     112     236       7

Cuts at:   2572    2639    2730    2766    3437    3758    4105    4342    4449 
   Size:         67      91      36     671     321     347     237     107

  Fragments arranged by size: 

                1000     671     585     512     347     321     237     236
                 112     107     107      91      67      36      13       7
            
 
AseI AT'TA_AT

Cuts at:      0    1859    2543    4449 
   Size:       1859     684    1906

  Fragments arranged by size: 

                1906    1859     684
 
AvaI C'yCGr_G

Cuts at:      0    1762    4427    4449 
   Size:       1762    2665      22

  Fragments arranged by size: 

                2665    1762      22
 
AvrII C'CTAG_G

Cuts at:      0    3949    4021    4449 
   Size:       3949      72     428

  Fragments arranged by size: 

                3949     428      72
 
BanI G'GyrC_C

Cuts at:      0      93     425     808    1380    1774    2989    4449 
   Size:         93     332     383     572     394    1215    1460

  Fragments arranged by size: 

                1460    1215     572     394     383     332      93
 
BanII G_rGCy'C

Cuts at:      0    2071    4449 
   Size:       2071    2378
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     979    1082    1252    2258    4318    4449 
   Size:        979     103     170    1006    2060     131

  Fragments arranged by size: 

                2060    1006     979     170     131     103
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2905    4377    4449 
   Size:       2905    1472      72

  Fragments arranged by size: 

                2905    1472      72
 
BfrBI ATG'CAT

Cuts at:      0    1825    1845    4449 
   Size:       1825      20    2604

  Fragments arranged by size: 

                2604    1825      20
 
Bme1580I G_kGCm'C

Cuts at:      0    2994    4449 
   Size:       2994    1455
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1202    2512    4449 
   Size:       1202    1310    1937

  Fragments arranged by size: 

                1937    1310    1202
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2293    4449 
   Size:       2293    2156
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1019    4449 
   Size:       1019    3430
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     295    1172    3557    4449 
   Size:        295     877    2385     892

  Fragments arranged by size: 

                2385     892     877     295
 
BseYI C'CCAG_C

Cuts at:      0    2994    4449 
   Size:       2994    1455
 
BsiHKAI G_wGCw'C

Cuts at:      0    2071    3220    4449 
   Size:       2071    1149    1229

  Fragments arranged by size: 

                2071    1229    1149
 
Bsp1286I G_dGCh'C

Cuts at:      0    2071    2994    3220    4449 
   Size:       2071     923     226    1229

  Fragments arranged by size: 

                2071    1229     923     226
 
BspHI T'CATG_A

Cuts at:      0    1241    4449 
   Size:       1241    3208
 
BsrDI GCAATG_nn'

Cuts at:      0     289    3502    3880    4449 
   Size:        289    3213     378     569

  Fragments arranged by size: 

                3213     569     378     289
 
BsrGI T'GTAC_A

Cuts at:      0     286    2198    4449 
   Size:        286    1912    2251

  Fragments arranged by size: 

                2251    1912     286
 
BssSI C'ACGA_G

Cuts at:      0    2458    2999    4449 
   Size:       2458     541    1450

  Fragments arranged by size: 

                2458    1450     541
 
BstBI TT'CG_AA

Cuts at:      0    2488    4449 
   Size:       2488    1961
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     655    4449 
   Size:        655    3794
 
BtgI C'CryG_G

Cuts at:      0     429    4449 
   Size:        429    4020
 
BtsI GCAGTG_nn'

Cuts at:      0    1979    2538    4449 
   Size:       1979     559    1911

  Fragments arranged by size: 

                1979    1911     559
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1689    1724    4449 
   Size:       1689      35    2725

  Fragments arranged by size: 

                2725    1689      35
 
DraI TTT'AAA

Cuts at:      0      50     365     495    2108    2129    2765    3320    3455 
   Size:         50     315     130    1613      21     636     555     135

Cuts at:   3455    4230    4449 
   Size:        775     219

  Fragments arranged by size: 

                1613     775     636     555     315     219     135     130
                  50      21
 
DraIII CAC_nnn'GTG

Cuts at:      0     604    4449 
   Size:        604    3845
 
EarI CTCTTCn'nnn_

Cuts at:      0     314    1208    4449 
   Size:        314     894    3241

  Fragments arranged by size: 

                3241     894     314
 
EcoICRI GAG'CTC

Cuts at:      0    2069    4449 
   Size:       2069    2380
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     335     730    1094    1232    2293    2344    2363    3428 
   Size:        335     395     364     138    1061      51      19    1065

Cuts at:   3428    3935    4449 
   Size:        507     514

  Fragments arranged by size: 

                1065    1061     514     507     395     364     335     138
                  51      19
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1261    3145    4449 
   Size:       1261    1884    1304

  Fragments arranged by size: 

                1884    1304    1261
 
EcoRV GAT'ATC

Cuts at:      0    1601    4449 
   Size:       1601    2848
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     760    1667    2015    3202    4449 
   Size:        760     907     348    1187    1247

  Fragments arranged by size: 

                1247    1187     907     760     348
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    3547    3579    4449 
   Size:       3547      32     870

  Fragments arranged by size: 

                3547     870      32
 
HincII GTy'rAC

Cuts at:      0     201    1728    1803    4449 
   Size:        201    1527      75    2646

  Fragments arranged by size: 

                2646    1527     201      75
 
KpnI G_GTAC'C

Cuts at:      0    1778    4449 
   Size:       1778    2671
 
MfeI C'AATT_G

Cuts at:      0    2353    4449 
   Size:       2353    2096
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1919    2046    4449 
   Size:       1919     127    2403

  Fragments arranged by size: 

                2403    1919     127
 
MslI CAynn'nnrTG

Cuts at:      0     866    1036    1106    1542    4449 
   Size:        866     170      70     436    2907

  Fragments arranged by size: 

                2907     866     436     170      70
 
MspA1I CmG'CkG

Cuts at:      0     359    2974    4449 
   Size:        359    2615    1475

  Fragments arranged by size: 

                2615    1475     359
 
NcoI C'CATG_G

Cuts at:      0     429    4449 
   Size:        429    4020
 
NsiI A_TGCA'T

Cuts at:      0    1827    1847    4449 
   Size:       1827      20    2602

  Fragments arranged by size: 

                2602    1827      20
 
NspI r_CATG'y

Cuts at:      0     841    3201    3641    4120    4443    4449 
   Size:        841    2360     440     479     323       6

  Fragments arranged by size: 

                2360     841     479     440     323       6
 
PciI A'CATG_T

Cuts at:      0     837    4116    4439    4449 
   Size:        837    3279     323      10

  Fragments arranged by size: 

                3279     837     323      10
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1464    3001    4449 
   Size:       1464    1537    1448

  Fragments arranged by size: 

                1537    1464    1448
 
PfoI T'CCnGG_A

Cuts at:      0    2859    4449 
   Size:       2859    1590
 
PpuMI rG'GwC_Cy

Cuts at:      0    1261    4449 
   Size:       1261    3188
 
PsiI TTA'TAA

Cuts at:      0     701     844    4178    4449 
   Size:        701     143    3334     271

  Fragments arranged by size: 

                3334     701     271     143
 
PstI C_TGCA'G

Cuts at:      0     927    4449 
   Size:        927    3522
 
PvuII CAG'CTG

Cuts at:      0     359    4449 
   Size:        359    4090
 
SacI G_AGCT'C

Cuts at:      0    2071    4449 
   Size:       2071    2378
 
ScaI AGT'ACT

Cuts at:      0    3378    4449 
   Size:       3378    1071
 
SfcI C'TryA_G

Cuts at:      0     923    4449 
   Size:        923    3526
 
SmlI C'TyrA_G

Cuts at:      0     931    1034    1042    2662    3352    4123    4449 
   Size:        931     103       8    1620     690     771     326

  Fragments arranged by size: 

                1620     931     771     690     326     103       8
 
SpeI A'CTAG_T

Cuts at:      0    2052    4449 
   Size:       2052    2397
 
SspI AAT'ATT

Cuts at:      0     168     796    3428    4329    4449 
   Size:        168     628    2632     901     120

  Fragments arranged by size: 

                2632     901     628     168     120
 
StyI C'CwwG_G

Cuts at:      0     429    1899    3949    4021    4449 
   Size:        429    1470    2050      72     428

  Fragments arranged by size: 

                2050    1470     429     428      72
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     661    4449 
   Size:        661    3788
 
TatI w'GTAC_w

Cuts at:      0     286    1254    2198    3376    4119    4449 
   Size:        286     968     944    1178     743     330

  Fragments arranged by size: 

                1178     968     944     743     330     286
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1958    1990    2879    2911    4449 
   Size:       1958      32     889      32    1538

  Fragments arranged by size: 

                1958    1538     889      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2670    4449 
   Size:       2670    1779
 
XbaI T'CTAG_A

Cuts at:      0    3565    3814    4449 
   Size:       3565     249     635

  Fragments arranged by size: 

                3565     635     249
 
XmnI GAAnn'nnTTC

Cuts at:      0     976    2494    4449 
   Size:        976    1518    1955

  Fragments arranged by size: 

                1955    1518     976
 

 Enzymes that do cut:

   Acc65I     AcuI    AflII   AflIII     AleI     AloI    AlwNI     ApoI
     AseI     AvaI    AvrII     BanI    BanII     BbsI    BciVI    BfrBI
 Bme1580I     BmrI     BpmI    BpuEI    BseRI    BseYI  BsiHKAI Bsp1286I
    BspHI    BsrDI    BsrGI    BssSI    BstBI    BstXI     BtgI     BtsI
    CspCI     DraI   DraIII     EarI  EcoICRI  Eco57MI EcoO109I    EcoRV
     FalI    Hin4I   HincII     KpnI     MfeI     MmeI     MslI   MspA1I
     NcoI     NsiI     NspI     PciI    PflMI     PfoI    PpuMI     PsiI
     PstI    PvuII     SacI     ScaI     SfcI     SmlI     SpeI     SspI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI     AgeI     AhdI     AlfI
     ApaI    ApaLI     AscI    AsiSI     BaeI    BamHI     BbeI    BbvCI
     BcgI     BclI     BglI    BglII     BlpI    BmgBI     BmtI     BplI
   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrFI
   BssHII   BstAPI   BstEII    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI
     DrdI     EaeI     EagI     EciI    EcoNI    EcoRI     FseI     FspI
    FspAI    HaeII  HindIII     HpaI     KasI     MluI     MscI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI
     PmeI     PmlI     PpiI    PshAI   PspOMI    PspXI     PsrI     PvuI
    RsrII    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SphI     SrfI     StuI
     SwaI     XcmI     XhoI     XmaI     ZraI
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