(Circular) (Six-base) MAPSORT of: pF1KA0259.seq Check: 6508 from: 1 to: 7679 pF1KA0259 7679 bp With 182 enzymes: SgfI * June 20, 2008 10:19 .. AatII G_ACGT'C Cuts at: 1627 2661 1627 Size: 1034 6645 AccI GT'mk_AC Cuts at: 512 3144 3215 4563 4717 512 Size: 2632 71 1348 154 3474 Fragments arranged by size: 3474 2632 1348 154 71 Acc65I G'GTAC_C Cuts at: 4695 4695 Size: 7679 AclI AA'CG_TT Cuts at: 7519 7519 Size: 7679 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 140 208 613 1193 1442 1649 1772 2527 3610 Size: 68 405 580 249 207 123 755 1083 Cuts at: 3610 3752 3776 4336 5504 5936 6289 6951 140 Size: 142 24 560 1168 432 353 662 868 Fragments arranged by size: 1168 1083 868 755 662 580 560 432 405 353 249 207 142 123 68 24 AflIII A'CryG_T Cuts at: 3723 4311 4487 4671 5031 6831 7112 3723 Size: 588 176 184 360 1800 281 4290 Fragments arranged by size: 4290 1800 588 360 281 184 176 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 2431 2431 Size: 7679 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1250 1282 1418 1450 2114 2146 1250 Size: 32 136 32 664 32 6783 Fragments arranged by size: 6783 664 136 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 2392 3499 4474 4984 6422 2392 Size: 1107 975 510 1438 3649 Fragments arranged by size: 3649 1438 1107 975 510 ApaLI G'TGCA_C Cuts at: 6517 6517 Size: 7679 ApoI r'AATT_y Cuts at: 202 1460 2241 2325 2382 2581 4683 7032 7288 Size: 1258 781 84 57 199 2102 2349 256 Cuts at: 7288 202 Size: 593 Fragments arranged by size: 2349 2102 1258 781 593 256 199 84 57 AseI AT'TA_AT Cuts at: 20 20 Size: 7679 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 7679 AvaI C'yCGr_G Cuts at: 187 4325 4699 187 Size: 4138 374 3167 Fragments arranged by size: 4138 3167 374 BamHI G'GATC_C Cuts at: 4704 4704 Size: 7679 BanI G'GyrC_C Cuts at: 4695 5109 5357 5392 4695 Size: 414 248 35 6982 Fragments arranged by size: 6982 414 248 35 BanII G_rGCy'C Cuts at: 1667 2555 4693 5723 1667 Size: 888 2138 1030 3623 Fragments arranged by size: 3623 2138 1030 888 BbeI G_GCGC'C Cuts at: 5113 5361 5113 Size: 248 7431 BbsI GAAGACnn'nnnn_ Cuts at: 146 1248 1703 3155 4154 4218 146 Size: 1102 455 1452 999 64 3607 Fragments arranged by size: 3607 1452 1102 999 455 64 BciVI GTATCCnnnnn_n' Cuts at: 2562 5577 6633 7679 2562 Size: 3015 1056 1046 2562 Fragments arranged by size: 3015 2562 1056 1046 BclI T'GATC_A Cuts at: 312 5199 312 Size: 4887 2792 BglI GCCn_nnn'nGGC Cuts at: 4072 4072 Size: 7679 BglII A'GATC_T Cuts at: 1572 5194 7231 1572 Size: 3622 2037 2020 Fragments arranged by size: 3622 2037 2020 BlpI GC'TnA_GC Cuts at: 1661 4791 1661 Size: 3130 4549 Bme1580I G_kGCm'C Cuts at: 3042 5304 5397 6521 3042 Size: 2262 93 1124 4200 Fragments arranged by size: 4200 2262 1124 93 BmrI ACTGGGnnnn_n' Cuts at: 2784 3806 4891 4972 5307 7451 2784 Size: 1022 1085 81 335 2144 3012 Fragments arranged by size: 3012 2144 1085 1022 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3500 4649 6046 3500 Size: 1149 1397 5133 Fragments arranged by size: 5133 1397 1149 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1138 2099 2636 1138 Size: 961 537 6181 Fragments arranged by size: 6181 961 537 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 335 3788 3923 4518 4855 5734 6448 6746 335 Size: 3453 135 595 337 879 714 298 1268 Fragments arranged by size: 3453 1268 879 714 595 337 298 135 BsaI GGTCTCn'nnnn_ Cuts at: 512 2496 7383 512 Size: 1984 4887 808 Fragments arranged by size: 4887 1984 808 BsaAI yAC'GTr Cuts at: 3724 5032 3724 Size: 1308 6371 BsaBI GATnn'nnATC Cuts at: 6 1571 5193 7230 6 Size: 1565 3622 2037 455 Fragments arranged by size: 3622 2037 1565 455 BsaHI Gr'CG_yC Cuts at: 1624 2658 5110 5358 6060 7556 1624 Size: 1034 2452 248 702 1496 1747 Fragments arranged by size: 2452 1747 1496 1034 702 248 BsaWI w'CCGG_w Cuts at: 4868 5389 6067 6478 6625 6899 4868 Size: 521 678 411 147 274 5648 Fragments arranged by size: 5648 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1250 1280 1922 1952 2114 2144 2542 2572 3328 Size: 30 642 30 162 30 398 30 756 Cuts at: 3328 3358 3720 3750 4355 4385 1250 Size: 30 362 30 605 30 4544 Fragments arranged by size: 4544 756 642 605 398 362 162 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 3014 3577 3895 4376 4532 3014 Size: 563 318 481 156 6161 Fragments arranged by size: 6161 563 481 318 156 BseYI C'CCAG_C Cuts at: 1843 1902 2523 2662 4078 5104 5862 6527 6935 Size: 59 621 139 1416 1026 758 665 408 Cuts at: 6935 1843 Size: 2587 Fragments arranged by size: 2587 1416 1026 758 665 621 408 139 59 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 4159 4159 Size: 7679 BsiEI CG_ry'CG Cuts at: 104 5221 5267 6497 104 Size: 5117 46 1230 1286 Fragments arranged by size: 5117 1286 1230 46 BsiHKAI G_wGCw'C Cuts at: 3023 4693 5471 5661 6521 3023 Size: 1670 778 190 860 4181 Fragments arranged by size: 4181 1670 860 778 190 Bsp1286I G_dGCh'C Cuts at: 1667 2555 3023 3042 4693 5304 5397 5471 5661 Size: 888 468 19 1651 611 93 74 190 Cuts at: 5661 5723 6521 1667 Size: 62 798 2825 Fragments arranged by size: 2825 1651 888 798 611 468 190 93 74 62 19 BspHI T'CATG_A Cuts at: 3888 6111 3888 Size: 2223 5456 BspMI ACCTGCnnnn'nnnn_ Cuts at: 2256 4729 4943 5245 5626 2256 Size: 2473 214 302 381 4309 Fragments arranged by size: 4309 2473 381 302 214 BsrBI CCG'CTC Cuts at: 50 5971 6025 7509 7674 50 Size: 5921 54 1484 165 55 Fragments arranged by size: 5921 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 4235 4511 5591 4235 Size: 276 1080 6323 Fragments arranged by size: 6323 1080 276 BsrFI r'CCGG_y Cuts at: 3128 5677 5858 3128 Size: 2549 181 4949 Fragments arranged by size: 4949 2549 181 BsrGI T'GTAC_A Cuts at: 445 489 820 882 4018 4200 445 Size: 44 331 62 3136 182 3924 Fragments arranged by size: 3924 3136 331 182 62 44 BssSI C'ACGA_G Cuts at: 5950 6658 5950 Size: 708 6971 BstAPI GCAn_nnn'nTGC Cuts at: 3577 4474 5056 3577 Size: 897 582 6200 Fragments arranged by size: 6200 897 582 BstBI TT'CG_AA Cuts at: 4047 6040 4047 Size: 1993 5686 BstXI CCAn_nnnn'nTGG Cuts at: 2077 2077 Size: 7679 BstYI r'GATC_y Cuts at: 1 1572 3186 4704 5171 5194 5529 5775 6081 Size: 1571 1614 1518 467 23 335 246 306 Cuts at: 6081 6093 6179 7231 1 Size: 12 86 1052 449 Fragments arranged by size: 1614 1571 1518 1052 467 449 335 306 246 86 23 12 BstZ17I GTA'TAC Cuts at: 513 513 Size: 7679 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 396 1803 4081 5629 5810 5973 396 Size: 1407 2278 1548 181 163 2102 Fragments arranged by size: 2278 2102 1548 1407 181 163 BtsI GCAGTG_nn' Cuts at: 3122 3453 3598 4006 4922 3122 Size: 331 145 408 916 5879 Fragments arranged by size: 5879 916 408 331 145 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 1593 1628 1593 Size: 35 7644 DraI TTT'AAA Cuts at: 149 180 1502 4401 4678 149 Size: 31 1322 2899 277 3150 Fragments arranged by size: 3150 2899 1322 277 31 DraIII CAC_nnn'GTG Cuts at: 891 902 4137 891 Size: 11 3235 4433 Fragments arranged by size: 4433 3235 11 DrdI GACnn_nn'nnGTC Cuts at: 5385 6729 5385 Size: 1344 6335 EaeI y'GGCC_r Cuts at: 2956 3126 3224 5264 5438 5829 5856 7201 2956 Size: 170 98 2040 174 391 27 1345 3434 Fragments arranged by size: 3434 2040 1345 391 174 170 98 27 EagI C'GGCC_G Cuts at: 5264 5264 Size: 7679 EarI CTCTTCn'nnn_ Cuts at: 236 2011 2608 5702 5912 236 Size: 1775 597 3094 210 2003 Fragments arranged by size: 3094 2003 1775 597 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 518 5887 6629 6775 7489 518 Size: 5369 742 146 714 708 Fragments arranged by size: 5369 742 714 708 146 EcoICRI GAG'CTC Cuts at: 4691 4691 Size: 7679 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 140 208 613 1138 1193 1442 1649 1772 2099 Size: 68 405 525 55 249 207 123 327 Cuts at: 2099 2527 2636 3610 3752 3776 4336 5504 5936 Size: 428 109 974 142 24 560 1168 432 Cuts at: 5936 6289 6951 140 Size: 353 662 868 Fragments arranged by size: 1168 974 868 662 560 525 432 428 405 353 327 249 207 142 123 109 68 55 24 EcoNI CCTnn'n_nnAGG Cuts at: 2590 2590 Size: 7679 EcoO109I rG'GnC_Cy Cuts at: 1637 4818 1637 Size: 3181 4498 EcoRI G'AATT_C Cuts at: 202 4683 202 Size: 4481 3198 EcoRV GAT'ATC Cuts at: 3108 3108 Size: 7679 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 238 1513 1551 2631 4020 5698 238 Size: 1275 38 1080 1389 1678 2219 Fragments arranged by size: 2219 1678 1389 1275 1080 38 FspI TGC'GCA Cuts at: 5460 5460 Size: 7679 HaeII r_GCGC'y Cuts at: 5113 5361 6591 7362 5113 Size: 248 1230 771 5430 Fragments arranged by size: 5430 1230 771 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1564 1596 1978 2010 2560 2592 2776 2808 2968 Size: 32 382 32 550 32 184 32 160 Cuts at: 2968 3000 3328 3360 3720 3752 5521 5551 5553 Size: 32 328 32 360 32 1769 30 2 Cuts at: 5553 5583 1564 Size: 30 3660 Fragments arranged by size: 3660 1769 550 382 360 328 184 160 32 32 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 3145 3507 3633 4718 3145 Size: 362 126 1085 6106 Fragments arranged by size: 6106 1085 362 126 HindIII A'AGCT_T Cuts at: 325 2474 325 Size: 2149 5530 KasI G'GCGC_C Cuts at: 5109 5357 5109 Size: 248 7431 KpnI G_GTAC'C Cuts at: 4699 4699 Size: 7679 MfeI C'AATT_G Cuts at: 802 1022 802 Size: 220 7459 MluI A'CGCG_T Cuts at: 7112 7112 Size: 7679 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1085 1174 2252 6438 6622 1085 Size: 89 1078 4186 184 2142 Fragments arranged by size: 4186 2142 1078 184 89 MscI TGG'CCA Cuts at: 3226 5440 3226 Size: 2214 5465 MslI CAynn'nnrTG Cuts at: 485 3724 4030 5668 5795 485 Size: 3239 306 1638 127 2369 Fragments arranged by size: 3239 2369 1638 306 127 MspA1I CmG'CkG Cuts at: 3157 4078 4790 5104 5464 6248 6493 3157 Size: 921 712 314 360 784 245 4343 Fragments arranged by size: 4343 921 784 712 360 314 245 NaeI GCC'GGC Cuts at: 3130 5860 3130 Size: 2730 4949 NarI GG'CG_CC Cuts at: 5110 5358 5110 Size: 248 7431 NgoMIV G'CCGG_C Cuts at: 3128 5858 3128 Size: 2730 4949 NspI r_CATG'y Cuts at: 2175 2503 4315 4732 5232 6835 2175 Size: 328 1812 417 500 1603 3019 Fragments arranged by size: 3019 1812 1603 500 417 328 PciI A'CATG_T Cuts at: 4311 6831 4311 Size: 2520 5159 PflMI CCAn_nnn'nTGG Cuts at: 875 902 1455 3256 875 Size: 27 553 1801 5298 Fragments arranged by size: 5298 1801 553 27 PfoI T'CCnGG_A Cuts at: 2652 3711 2652 Size: 1059 6620 PmeI GTTT'AAAC Cuts at: 4678 4678 Size: 7679 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1250 1282 2114 2146 6125 6157 1250 Size: 32 832 32 3979 32 2772 Fragments arranged by size: 3979 2772 832 32 32 32 PshAI GACnn'nnGTC Cuts at: 3956 3956 Size: 7679 PsiI TTA'TAA Cuts at: 413 3086 3871 413 Size: 2673 785 4221 Fragments arranged by size: 4221 2673 785 PstI C_TGCA'G Cuts at: 337 2397 4120 4726 5411 337 Size: 2060 1723 606 685 2605 Fragments arranged by size: 2605 2060 1723 685 606 PvuI CG_AT'CG Cuts at: 104 104 Size: 7679 PvuII CAG'CTG Cuts at: 3157 4078 5104 5464 3157 Size: 921 1026 360 5372 Fragments arranged by size: 5372 1026 921 360 RsrII CG'GwC_CG Cuts at: 5874 5874 Size: 7679 SacI G_AGCT'C Cuts at: 4693 4693 Size: 7679 SalI G'TCGA_C Cuts at: 3143 4716 3143 Size: 1573 6106 SapI GCTCTTCn'nnn_ Cuts at: 5702 5912 5702 Size: 210 7469 SbfI CC_TGCA'GG Cuts at: 4120 4726 4120 Size: 606 7073 ScaI AGT'ACT Cuts at: 2184 7196 2184 Size: 5012 2667 SexAI A'CCwGG_T Cuts at: 4377 4377 Size: 7679 SfcI C'TryA_G Cuts at: 33 333 2258 2393 3551 4116 4637 4722 5407 Size: 300 1925 135 1158 565 521 85 685 Cuts at: 5407 6375 6566 33 Size: 968 191 1146 Fragments arranged by size: 1925 1158 1146 968 685 565 521 300 191 135 85 SfoI GGC'GCC Cuts at: 5111 5359 5111 Size: 248 7431 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 7679 SmaI CCC'GGG Cuts at: 4701 4701 Size: 7679 SmlI C'TyrA_G Cuts at: 187 350 3803 3938 4325 4497 4834 5749 6463 Size: 163 3453 135 387 172 337 915 714 Cuts at: 6463 6725 187 Size: 262 1141 Fragments arranged by size: 3453 1141 915 714 387 337 262 172 163 135 SpeI A'CTAG_T Cuts at: 67 67 Size: 7679 SphI G_CATG'C Cuts at: 4732 5232 4732 Size: 500 7179 SspI AAT'ATT Cuts at: 574 1056 574 Size: 482 7197 StuI AGG'CCT Cuts at: 1362 1362 Size: 7679 StyI C'CwwG_G Cuts at: 2118 2591 2642 3369 4813 5167 2118 Size: 473 51 727 1444 354 4630 Fragments arranged by size: 4630 1444 727 473 354 51 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 3761 4077 4283 5262 5395 5854 6063 3761 Size: 316 206 979 133 459 209 5377 Fragments arranged by size: 5377 979 459 316 209 206 133 TatI w'GTAC_w Cuts at: 445 489 820 882 2182 3003 3908 4018 4200 Size: 44 331 62 1300 821 905 110 182 Cuts at: 4200 4490 4668 7194 445 Size: 290 178 2526 930 Fragments arranged by size: 2526 1300 930 905 821 331 290 182 178 110 62 44 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2001 2033 2998 3030 4121 4153 4355 4387 7196 Size: 32 965 32 1091 32 202 32 2809 Cuts at: 7196 7228 2001 Size: 32 2452 Fragments arranged by size: 2809 2452 1091 965 202 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 5476 5476 Size: 7679 XbaI T'CTAG_A Cuts at: 1137 4710 1137 Size: 3573 4106 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3099 3840 5174 3099 Size: 741 1334 5604 Fragments arranged by size: 5604 1334 741 XhoI C'TCGA_G Cuts at: 187 4325 187 Size: 4138 3541 XmaI C'CCGG_G Cuts at: 4699 4699 Size: 7679 XmnI GAAnn'nnTTC Cuts at: 221 476 4167 221 Size: 255 3691 3733 Fragments arranged by size: 3733 3691 255 ZraI GAC'GTC Cuts at: 1625 2659 1625 Size: 1034 6645 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflIII AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AflII AgeI AhdI AleI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsiWI BsmI BsmBI BspEI BssHII BstEII Bsu36I BtgI ClaI FseI FspAI HpaI NcoI NdeI NheI NotI NruI NsiI PacI PasI PmlI PpuMI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SwaI