Restriction map for
pF1KA0177
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# NO CUT ENZYMES ( 58 enzymes) #
##################################
Acc65I AclI AgeI Aor51HI ApaI
AscI AseI AsnI Asp718I AviII
AvrII BbeI BcgI BlnI BlpI
Bpu1102I BsmBI BspDI BspLU11I BspMI
BsrBI BssHII Bst1107I BstEII BstPI
BstZ17I CelII ClaI CpoI DraI
DraIII DrdI Eco47III EcoO65I FspI
HpaI KasI KpnI MluI NarI
NdeI NheI NotI NruI PacI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SapI SfoI SgrAI
SnaBI SpeI SwaI
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# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AatII 1402 GACGTC GACGT^C
AccIII 2130 TCCGGA T^CCGGA
AflII 3036 CTTAAG C^TTAAG
AflIII 3837 ACGTGT A^C[GA][CT]GT
AhdI 3248 GACTCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 3248 GACTCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 4127 GGATCC G^GATCC
BbrPI 1948 CACGTG CAC^GTG
BbvCI 55 CCTCAGC CCTCAGC(-5/-2)
BfrI 3036 CTTAAG C^TTAAG
BglI 3202 GCCCTGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 4488 AGATCT A^GATCT
BsaBI 6 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 589 GAGACC GGTCTC(1/5)
BsaWI 2130 TCCGGA [AT]^CCGG[AT]
BseAI 2130 TCCGGA T^CCGGA
BsiEI 3962 CGGCCG CG[GA][CT]^CG
BsiWI 2375 CGTACG C^GTACG
BspEI 2130 TCCGGA T^CCGGA
BsrFI 1831 GCCGGC [GA]^CCGG[CT]
BssSI 2965 CTCGTG CACGAG(-5/-1)
BstBI 2008 TTCGAA TT^CGAA
Cfr10I 1831 GCCGGC [GA]^CCGG[CT]
EagI 3962 CGGCCG C^GGCCG
Eam1105I 3248 GACTCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3962 CGGCCG C^GGCCG
Eco52I 3962 CGGCCG C^GGCCG
EcoT22I 4079 ATGCAT ATGCA^T
FseI 1830 GGCCGGCC GGCCGG^CC
HaeII 2104 GGCGCT [GA]GCGC^[CT]
HgaI 4141 GCGTC GACGC(5/10)
KspI 2804 CCGCGG CCGC^GG
MamI 6 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2130 TCCGGA T^CCGGA
NaeI 1831 GCCGGC GCC^GGC
NgoMIV 1831 GCCGGC G^CCGGC
NsiI 4079 ATGCAT ATGCA^T
NspV 2008 TTCGAA TT^CGAA
PaeR7I 2897 CTCGAG C^TCGAG
PflMI 3372 CCACAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1948 CACGTG CAC^GTG
PmlI 1948 CACGTG CAC^GTG
PpuMI 4155 AGGACCT [GA]G^G[AT]CC[CT]
PstI 3205 CTGCAG CTGCA^G
SacII 2804 CCGCGG CCGC^GG
SbfI 3204 CCTGCAGG CCTGCA^GG
SfiI 3201 GGCCCTGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 2008 TTCGAA TT^CGAA
SmaI 1470 CCCGGG CCC^GGG
Sse8387I 3204 CCTGCAGG CCTGCA^GG
StuI 4267 AGGCCT AGG^CCT
Van91I 3372 CCACAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 4500 TCTAGA T^CTAGA
XhoI 2897 CTCGAG C^TCGAG
XmaCI 1470 CCCGGG C^CCGGG
XmaI 1470 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcyI 1402 GACGTC G[GA]^CG[CT]C
4140 GGCGTC
AspI 2355 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3867 GACAGAGTC
AvaII 4156 GGACC G^G[AT]CC
4872 GGACC
BalI 1115 TGGCCA TGG^CCA
3688 TGGCCA
BclI 312 TGATCA T^GATCA
1731 TGATCA
BsaAI 1948 CACGTG [CT]AC^GT[GA]
2377 TACGTG
BsaHI 1402 GACGTC G[GA]^CG[CT]C
4140 GGCGTC
BsmI 3416 GCATTC GAATGC(1/-1)
4832 GCATTC
Bsp1407I 1323 TGTACA T^GTACA
3322 TGTACA
BsrDI 740 CATTGC GCAATG(2/0)
3239 GCAATG
BsrGI 1323 TGTACA T^GTACA
3322 TGTACA
BstYI 4127 GGATCC [GA]^GATC[CT]
4488 AGATCT
EcoNI 985 CCTCACAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2893 CCTGCTCGAGG
EcoRV 555 GATATC GAT^ATC
2873 GATATC
FbaI 312 TGATCA T^GATCA
1731 TGATCA
Hin1I 1402 GACGTC G[GA]^CG[CT]C
4140 GGCGTC
HincII 2596 GTCGAC GT[CT]^[GA]AC
3333 GTCGAC
HindII 2596 GTCGAC GT[CT]^[GA]AC
3333 GTCGAC
HindIII 2343 AAGCTT A^AGCTT
3519 AAGCTT
MfeI 730 CAATTG C^AATTG
946 CAATTG
MflI 4127 GGATCC [GA]^GATC[CT]
4488 AGATCT
MluNI 1115 TGGCCA TGG^CCA
3688 TGGCCA
MscI 1115 TGGCCA TGG^CCA
3688 TGGCCA
MslI 1723 CATCCTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3424 CACGAAAATG
MunI 730 CAATTG C^AATTG
946 CAATTG
PflFI 2355 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3867 GACAGAGTC
PvuII 4055 CAGCTG CAG^CTG
4602 CAGCTG
SacI 3389 GAGCTC GAGCT^C
4209 GAGCTC
SalI 2596 GTCGAC G^TCGAC
3333 GTCGAC
SexAI 2989 ACCAGGT A^CC[AT]GGT
4912 ACCAGGT
SphI 2567 GCATGC GCATG^C
2700 GCATGC
SspBI 1323 TGTACA T^GTACA
3322 TGTACA
Tth111I 2355 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3867 GACAGAGTC
XhoII 4127 GGATCC [GA]^GATC[CT]
4488 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI Alw44I AlwI AlwNI AocI
ApaLI ApoI Asp700I AspHI AvaI
BanI BanII BbsI BbvI BciVI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspHI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI RcaI
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XmnI