Restriction map for
pF1KA0177
(Circular) (Six-base) MAPSORT of: pF1KA0177.seq Check: 2974 from: 1 to: 8285
pF1KA0177 8285 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AatII G_ACGT'C
Cuts at: 1514 1514
Size: 8285
AccI GT'mk_AC
Cuts at: 2705 3442 3716 4842 5323 2705
Size: 737 274 1126 481 5667
Fragments arranged by size:
5667 1126 737 481 274
Acc65I G'GTAC_C
Cuts at: 5301 5301
Size: 8285
AclI AA'CG_TT
Cuts at: 8125 8125
Size: 8285
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 461 610 752 793 833 1295 1691 2386 3439
Size: 149 142 41 40 462 396 695 1053
Cuts at: 3439 3902 5165 6110 6542 6895 7557 461
Size: 463 1263 945 432 353 662 1189
Fragments arranged by size:
1263 1189 1053 945 695 662 463 462
432 396 353 149 142 41 40
AflII C'TTAA_G
Cuts at: 3144 3144
Size: 8285
AflIII A'CryG_T
Cuts at: 3945 5637 7437 7718 3945
Size: 1692 1800 281 4512
Fragments arranged by size:
4512 1800 1692 281
AhdI GACnn_n'nnGTC
Cuts at: 3361 3361
Size: 8285
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1170 1170
Size: 8285
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2306 2338 2306
Size: 32 8253
AlwNI CAG_nnn'CTG
Cuts at: 650 1720 4000 4441 5217 5590 7028 650
Size: 1070 2280 441 776 373 1438 1907
Fragments arranged by size:
2280 1907 1438 1070 776 441 373
ApaLI G'TGCA_C
Cuts at: 240 3404 4794 7123 240
Size: 3164 1390 2329 1402
Fragments arranged by size:
3164 2329 1402 1390
ApoI r'AATT_y
Cuts at: 291 530 870 1279 1747 1795 1878 2494 3424
Size: 239 340 409 468 48 83 616 930
Cuts at: 3424 3833 3930 5289 7638 7894 291
Size: 409 97 1359 2349 256 682
Fragments arranged by size:
2349 1359 930 682 616 468 409 409
340 256 239 97 83 48
AseI AT'TA_AT
Cuts at: 20 20
Size: 8285
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8285
AvaI C'yCGr_G
Cuts at: 1578 3005 3304 3494 5305 1578
Size: 1427 299 190 1811 4558
Fragments arranged by size:
4558 1811 1427 299 190
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4993 5026 4993
Size: 33 8252
BamHI G'GATC_C
Cuts at: 4235 5310 4235
Size: 1075 7210
BanI G'GyrC_C
Cuts at: 1594 3041 3166 3330 4387 5301 5715 5963 5998
Size: 1447 125 164 1057 914 414 248 35
Cuts at: 5998 1594
Size: 3881
Fragments arranged by size:
3881 1447 1057 914 414 248 164 125
35
BanII G_rGCy'C
Cuts at: 1330 3501 4321 5299 6329 1330
Size: 2171 820 978 1030 3286
Fragments arranged by size:
3286 2171 1030 978 820
BbeI G_GCGC'C
Cuts at: 5719 5967 5719
Size: 248 8037
BbsI GAAGACnn'nnnn_
Cuts at: 494 711 3233 3466 4597 494
Size: 217 2522 233 1131 4182
Fragments arranged by size:
4182 2522 1131 233 217
BbvCI CC'TCA_GC
Cuts at: 164 164
Size: 8285
BciVI GTATCCnnnnn_n'
Cuts at: 2400 2897 4762 6183 7239 8285 2400
Size: 497 1865 1421 1056 1046 2400
Fragments arranged by size:
2400 1865 1421 1056 1046 497
BclI T'GATC_A
Cuts at: 420 1839 5805 420
Size: 1419 3966 2900
Fragments arranged by size:
3966 2900 1419
BfrBI ATG'CAT
Cuts at: 4189 4189
Size: 8285
BglI GCCn_nnn'nGGC
Cuts at: 3316 3316
Size: 8285
BglII A'GATC_T
Cuts at: 4596 5800 7837 4596
Size: 1204 2037 5044
Fragments arranged by size:
5044 2037 1204
BlpI GC'TnA_GC
Cuts at: 5397 5397
Size: 8285
Bme1580I G_kGCm'C
Cuts at: 244 2063 2332 3044 3305 3408 4798 5910 6003
Size: 1819 269 712 261 103 1390 1112 93
Cuts at: 6003 7127 244
Size: 1124 1402
Fragments arranged by size:
1819 1402 1390 1124 1112 712 269 261
103 93
BmgBI CAC'GTC
Cuts at: 3946 3946
Size: 8285
BmrI ACTGGGnnnn_n'
Cuts at: 1324 3153 4585 4891 5191 5202 5497 5578 5913
Size: 1829 1432 306 300 11 295 81 335
Cuts at: 5913 8057 1324
Size: 2144 1552
Fragments arranged by size:
2144 1829 1552 1432 335 306 300 295
81 11
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4990 6652 4990
Size: 1662 6623
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 627 1829 1930 1996 2742 3031 4379 5209 627
Size: 1202 101 66 746 289 1348 830 3703
Fragments arranged by size:
3703 1348 1202 830 746 289 101 66
Bpu10I CC'TnA_GC
Cuts at: 164 2282 2891 4397 164
Size: 2118 609 1506 4052
Fragments arranged by size:
4052 2118 1506 609
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 530 940 5461 6340 7054 7352 530
Size: 410 4521 879 714 298 1463
Fragments arranged by size:
4521 1463 879 714 410 298
BsaI GGTCTCn'nnnn_
Cuts at: 691 7989 691
Size: 7298 987
BsaAI yAC'GTr
Cuts at: 2058 2487 5638 2058
Size: 429 3151 4705
Fragments arranged by size:
4705 3151 429
BsaBI GATnn'nnATC
Cuts at: 6 118 5799 7836 6
Size: 112 5681 2037 455
Fragments arranged by size:
5681 2037 455 112
BsaHI Gr'CG_yC
Cuts at: 1511 4249 5716 5964 6666 8162 1511
Size: 2738 1467 248 702 1496 1634
Fragments arranged by size:
2738 1634 1496 1467 702 248
BsaWI w'CCGG_w
Cuts at: 2238 5474 5995 6673 7084 7231 7505 2238
Size: 3236 521 678 411 147 274 3018
Fragments arranged by size:
3236 3018 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 399 429 613 643 662 692 3253 3283 4251
Size: 30 184 30 19 30 2561 30 968
Cuts at: 4251 4281 399
Size: 30 4403
Fragments arranged by size:
4403 2561 968 184 30 30 30 30
30 19
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 496 3096 4160 4825 496
Size: 2600 1064 665 3956
Fragments arranged by size:
3956 2600 1064 665
BseYI C'CCAG_C
Cuts at: 2174 3321 4412 5217 5710 6468 7133 7541 2174
Size: 1147 1091 805 493 758 665 408 2918
Fragments arranged by size:
2918 1147 1091 805 758 665 493 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 3369 4271 4316 3369
Size: 902 45 7338
Fragments arranged by size:
7338 902 45
BsiEI CG_ry'CG
Cuts at: 104 4073 5827 5873 7103 104
Size: 3969 1754 46 1230 1286
Fragments arranged by size:
3969 1754 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 244 3408 3501 4321 4798 5299 6077 6267 7127
Size: 3164 93 820 477 501 778 190 860
Cuts at: 7127 244
Size: 1402
Fragments arranged by size:
3164 1402 860 820 778 501 477 190
93
BsiWI C'GTAC_G
Cuts at: 2483 2483
Size: 8285
BsmI GAATG_Cn'
Cuts at: 3524 4940 3524
Size: 1416 6869
Bsp1286I G_dGCh'C
Cuts at: 244 1330 2063 2332 3044 3305 3408 3501 4321
Size: 1086 733 269 712 261 103 93 820
Cuts at: 4321 4798 5299 5910 6003 6077 6267 6329 7127
Size: 477 501 611 93 74 190 62 798
Cuts at: 7127 244
Size: 1402
Fragments arranged by size:
1402 1086 820 798 733 712 611 501
477 269 261 190 103 93 93 74
62
BspEI T'CCGG_A
Cuts at: 2238 2238
Size: 8285
BspHI T'CATG_A
Cuts at: 866 2514 3549 6717 866
Size: 1648 1035 3168 2434
Fragments arranged by size:
3168 2434 1648 1035
BspMI ACCTGCnnnn'nnnn_
Cuts at: 5335 5549 5851 6232 5335
Size: 214 302 381 7388
Fragments arranged by size:
7388 381 302 214
BsrBI CCG'CTC
Cuts at: 50 6577 6631 8115 8280 50
Size: 6527 54 1484 165 55
Fragments arranged by size:
6527 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 847 3354 6197 847
Size: 2507 2843 2935
Fragments arranged by size:
2935 2843 2507
BsrFI r'CCGG_y
Cuts at: 1939 6283 6464 1939
Size: 4344 181 3760
Fragments arranged by size:
4344 3760 181
BsrGI T'GTAC_A
Cuts at: 1431 3430 1431
Size: 1999 6286
BssSI C'ACGA_G
Cuts at: 3073 6556 7264 3073
Size: 3483 708 4094
Fragments arranged by size:
4094 3483 708
BstAPI GCAn_nnn'nTGC
Cuts at: 849 2540 4339 5662 849
Size: 1691 1799 1323 3472
Fragments arranged by size:
3472 1799 1691 1323
BstBI TT'CG_AA
Cuts at: 2117 6646 2117
Size: 4529 3756
BstXI CCAn_nnnn'nTGG
Cuts at: 114 1482 2209 2636 2766 4998 114
Size: 1368 727 427 130 2232 3401
Fragments arranged by size:
3401 2232 1368 727 427 130
BstYI r'GATC_y
Cuts at: 1 4235 4596 5310 5777 5800 6135 6381 6687
Size: 4234 361 714 467 23 335 246 306
Cuts at: 6687 6699 6785 7837 1
Size: 12 86 1052 449
Fragments arranged by size:
4234 1052 714 467 449 361 335 306
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2276 3903 4531 4559 2276
Size: 1627 628 28 6002
Fragments arranged by size:
6002 1627 628 28
BtgI C'CryG_G
Cuts at: 107 2208 2561 2765 2912 2948 4027 5170 107
Size: 2101 353 204 147 36 1079 1143 3222
Fragments arranged by size:
3222 2101 1143 1079 353 204 147 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 6235 6416 6579 6235
Size: 181 163 7941
Fragments arranged by size:
7941 181 163
BtsI GCAGTG_nn'
Cuts at: 2592 3381 4345 5528 2592
Size: 789 964 1183 5349
Fragments arranged by size:
5349 1183 964 789
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1716 1751 3614 3649 1716
Size: 35 1863 35 6352
Fragments arranged by size:
6352 1863 35 35
DraI TTT'AAA
Cuts at: 5284 5284
Size: 8285
DrdI GACnn_nn'nnGTC
Cuts at: 5991 7335 5991
Size: 1344 6941
EaeI y'GGCC_r
Cuts at: 1223 3796 4070 5870 6044 6435 6462 7807 1223
Size: 2573 274 1800 174 391 27 1345 1701
Fragments arranged by size:
2573 1800 1701 1345 391 274 174 27
EagI C'GGCC_G
Cuts at: 4070 5870 4070
Size: 1800 6485
EarI CTCTTCn'nnn_
Cuts at: 3073 3887 4433 4772 4959 5255 6308 6518 3073
Size: 814 546 339 187 296 1053 210 4840
Fragments arranged by size:
4840 1053 814 546 339 296 210 187
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 224 6493 7235 7381 8095 224
Size: 6269 742 146 714 414
Fragments arranged by size:
6269 742 714 414 146
EcoICRI GAG'CTC
Cuts at: 3499 4319 5297 3499
Size: 820 978 6487
Fragments arranged by size:
6487 978 820
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 461 610 627 752 793 833 1295 1691 1829
Size: 149 17 125 41 40 462 396 138
Cuts at: 1829 1930 1996 2386 2742 3031 3439 3902 4379
Size: 101 66 390 356 289 408 463 477
Cuts at: 4379 5165 5209 6110 6542 6895 7557 461
Size: 786 44 901 432 353 662 1189
Fragments arranged by size:
1189 901 786 662 477 463 462 432
408 396 390 356 353 289 149 138
125 101 66 44 41 40 17
EcoNI CCTnn'n_nnAGG
Cuts at: 1097 3005 1097
Size: 1908 6377
EcoO109I rG'GnC_Cy
Cuts at: 927 1409 3309 3318 4207 4264 5424 927
Size: 482 1900 9 889 57 1160 3788
Fragments arranged by size:
3788 1900 1160 889 482 57 9
EcoRI G'AATT_C
Cuts at: 291 870 2494 5289 291
Size: 579 1624 2795 3287
Fragments arranged by size:
3287 2795 1624 579
EcoRV GAT'ATC
Cuts at: 665 2983 665
Size: 2318 5967
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 237 381 868 2469 2474 2687 6304 237
Size: 144 487 1601 5 213 3617 2218
Fragments arranged by size:
3617 2218 1601 487 213 144 5
FseI GG_CCGG'CC
Cuts at: 1943 1943
Size: 8285
FspI TGC'GCA
Cuts at: 6066 6066
Size: 8285
HaeII r_GCGC'y
Cuts at: 2216 5719 5967 7197 7968 2216
Size: 3503 248 1230 771 2533
Fragments arranged by size:
3503 2533 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1592 1624 2582 2614 2730 2762 3347 3379 3488
Size: 32 958 32 116 32 585 32 109
Cuts at: 3488 3520 3646 3678 4634 4666 4675 4707 5182
Size: 32 126 32 956 32 9 32 475
Cuts at: 5182 5214 6127 6157 6159 6189 1592
Size: 32 913 30 2 30 3688
Fragments arranged by size:
3688 958 956 913 585 475 126 116
109 32 32 32 32 32 32 32
32 32 30 30 9 2
HincII GTy'rAC
Cuts at: 2706 3443 5324 2706
Size: 737 1881 5667
Fragments arranged by size:
5667 1881 737
HindIII A'AGCT_T
Cuts at: 2451 3627 2451
Size: 1176 7109
KasI G'GCGC_C
Cuts at: 5715 5963 5715
Size: 248 8037
KpnI G_GTAC'C
Cuts at: 5305 5305
Size: 8285
MfeI C'AATT_G
Cuts at: 838 1054 838
Size: 216 8069
MluI A'CGCG_T
Cuts at: 7718 7718
Size: 8285
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1494 2127 2241 3190 3952 5006 7044 7228 1494
Size: 633 114 949 762 1054 2038 184 2551
Fragments arranged by size:
2551 2038 1054 949 762 633 184 114
MscI TGG'CCA
Cuts at: 1225 3798 6046 1225
Size: 2573 2248 3464
Fragments arranged by size:
3464 2573 2248
MslI CAynn'nnrTG
Cuts at: 112 1835 3536 6274 6401 112
Size: 1723 1701 2738 127 1996
Fragments arranged by size:
2738 1996 1723 1701 127
MspA1I CmG'CkG
Cuts at: 2104 2914 4165 4712 5396 5710 6070 6854 7099
Size: 810 1251 547 684 314 360 784 245
Cuts at: 7099 2104
Size: 3290
Fragments arranged by size:
3290 1251 810 784 684 547 360 314
245
NaeI GCC'GGC
Cuts at: 1941 6466 1941
Size: 4525 3760
NarI GG'CG_CC
Cuts at: 5716 5964 5716
Size: 248 8037
NcoI C'CATG_G
Cuts at: 107 2208 2561 2765 2948 4027 107
Size: 2101 353 204 183 1079 4365
Fragments arranged by size:
4365 2101 1079 353 204 183
NgoMIV G'CCGG_C
Cuts at: 1939 6464 1939
Size: 4525 3760
NsiI A_TGCA'T
Cuts at: 4191 4191
Size: 8285
NspI r_CATG'y
Cuts at: 951 1261 2679 2812 5338 5838 7441 951
Size: 310 1418 133 2526 500 1603 1795
Fragments arranged by size:
2526 1795 1603 1418 500 310 133
PasI CC'CwG_GG
Cuts at: 930 2202 5096 930
Size: 1272 2894 4119
Fragments arranged by size:
4119 2894 1272
PciI A'CATG_T
Cuts at: 7437 7437
Size: 8285
PflMI CCAn_nnn'nTGG
Cuts at: 3486 3486
Size: 8285
PfoI T'CCnGG_A
Cuts at: 680 1946 3047 4356 4579 4976 5083 680
Size: 1266 1101 1309 223 397 107 3882
Fragments arranged by size:
3882 1309 1266 1101 397 223 107
PmeI GTTT'AAAC
Cuts at: 5284 5284
Size: 8285
PmlI CAC'GTG
Cuts at: 2058 2058
Size: 8285
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6731 6763 6731
Size: 32 8253
PpuMI rG'GwC_Cy
Cuts at: 4264 4264
Size: 8285
PsiI TTA'TAA
Cuts at: 392 2293 392
Size: 1901 6384
PspXI vC'TCGA_Gb
Cuts at: 3005 3005
Size: 8285
PstI C_TGCA'G
Cuts at: 3317 5332 6017 3317
Size: 2015 685 5585
Fragments arranged by size:
5585 2015 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8285
PvuII CAG'CTG
Cuts at: 4165 4712 5710 6070 4165
Size: 547 998 360 6380
Fragments arranged by size:
6380 998 547 360
RsrII CG'GwC_CG
Cuts at: 6480 6480
Size: 8285
SacI G_AGCT'C
Cuts at: 3501 4321 5299 3501
Size: 820 978 6487
Fragments arranged by size:
6487 978 820
SacII CC_GC'GG
Cuts at: 2915 2915
Size: 8285
SalI G'TCGA_C
Cuts at: 2704 3441 5322 2704
Size: 737 1881 5667
Fragments arranged by size:
5667 1881 737
SapI GCTCTTCn'nnn_
Cuts at: 6308 6518 6308
Size: 210 8075
SbfI CC_TGCA'GG
Cuts at: 3317 5332 3317
Size: 2015 6270
ScaI AGT'ACT
Cuts at: 157 1570 4700 7802 157
Size: 1413 3130 3102 640
Fragments arranged by size:
3130 3102 1413 640
SexAI A'CCwGG_T
Cuts at: 3097 5020 3097
Size: 1923 6362
SfcI C'TryA_G
Cuts at: 33 2012 3125 3313 4433 4843 5005 5328 6013
Size: 1979 1113 188 1120 410 162 323 685
Cuts at: 6013 6981 7172 33
Size: 968 191 1146
Fragments arranged by size:
1979 1146 1120 1113 968 685 410 323
191 188 162
SfiI GGCCn_nnn'nGGCC
Cuts at: 3316 3316
Size: 8285
SfoI GGC'GCC
Cuts at: 5717 5965 5717
Size: 248 8037
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8285
SmaI CCC'GGG
Cuts at: 1580 5307 1580
Size: 3727 4558
SmlI C'TyrA_G
Cuts at: 545 955 3005 3144 5440 6355 7069 7331 545
Size: 410 2050 139 2296 915 714 262 1499
Fragments arranged by size:
2296 2050 1499 915 714 410 262 139
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8285
SphI G_CATG'C
Cuts at: 2679 2812 5338 5838 2679
Size: 133 2526 500 5126
Fragments arranged by size:
5126 2526 500 133
SspI AAT'ATT
Cuts at: 2847 3184 5091 2847
Size: 337 1907 6041
Fragments arranged by size:
6041 1907 337
StuI AGG'CCT
Cuts at: 4377 4377
Size: 8285
StyI C'CwwG_G
Cuts at: 107 575 1521 1934 2208 2561 2765 2792 2819
Size: 468 946 413 274 353 204 27 27
Cuts at: 2819 2948 4027 4203 4822 5419 5773 107
Size: 129 1079 176 619 597 354 2619
Fragments arranged by size:
2619 1079 946 619 597 468 413 354
353 274 204 176 129 27 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2815 5868 6001 6460 6669 2815
Size: 3053 133 459 209 4431
Fragments arranged by size:
4431 3053 459 209 133
TatI w'GTAC_w
Cuts at: 155 1431 1555 1568 3430 3909 4698 7800 155
Size: 1276 124 13 1862 479 789 3102 640
Fragments arranged by size:
3102 1862 1276 789 640 479 124 13
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 399 431 613 645 662 694 3005 3037 3253
Size: 32 182 32 17 32 2311 32 216
Cuts at: 3253 3285 4251 4283 7802 7834 399
Size: 32 966 32 3519 32 850
Fragments arranged by size:
3519 2311 966 850 216 182 32 32
32 32 32 32 32 17
Tth111I GACn'n_nGTC
Cuts at: 2466 3978 6082 2466
Size: 1512 2104 4669
Fragments arranged by size:
4669 2104 1512
XbaI T'CTAG_A
Cuts at: 4608 5316 4608
Size: 708 7577
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 335 1954 2946 3852 5214 5780 335
Size: 1619 992 906 1362 566 2840
Fragments arranged by size:
2840 1619 1362 992 906 566
XhoI C'TCGA_G
Cuts at: 3005 3005
Size: 8285
XmaI C'CCGG_G
Cuts at: 1578 5305 1578
Size: 3727 4558
XmnI GAAnn'nnTTC
Cuts at: 216 1280 2399 4700 216
Size: 1064 1119 2301 3801
Fragments arranged by size:
3801 2301 1119 1064
ZraI GAC'GTC
Cuts at: 1512 1512
Size: 8285
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AhdI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PsiI PspXI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AgeI AleI ApaI AscI AvrII BcgI
BmtI BsmBI BssHII BstEII BstZ17I ClaI DraIII FspAI
HpaI NdeI NheI NotI NruI PacI PshAI PspOMI
PsrI SanDI SgrAI SnaBI SrfI SwaI