Restriction map for pF1KA0160
 (Linear) (Six-base) MAPSORT of: KIAA0160.seq  Check: 8851  from: 1  to: 2217



KIAA0160.orf

 With 182 enzymes: SgfI * 

                        July 23, 2007 10:22  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     624    2217 
   Size:        624    1593
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1346    1658    2126    2217 
   Size:       1346     312     468      91

  Fragments arranged by size: 

                1346     468     312      91
 
AflIII A'CryG_T

Cuts at:      0     350    2217 
   Size:        350    1867
 
AgeI A'CCGG_T

Cuts at:      0     207    2217 
   Size:        207    2010
 
AleI CACnn'nnGTG

Cuts at:      0      20    2217 
   Size:         20    2197
 
ApaI G_GGCC'C

Cuts at:      0      47    2217 
   Size:         47    2170
 
ApoI r'AATT_y

Cuts at:      0     537     675     706    1140    1624    1950    2217 
   Size:        537     138      31     434     484     326     267

  Fragments arranged by size: 

                 537     484     434     326     267     138      31
 
AseI AT'TA_AT

Cuts at:      0     797    2217 
   Size:        797    1420
 
AvaI C'yCGr_G

Cuts at:      0      39    2217 
   Size:         39    2178
 
BamHI G'GATC_C

Cuts at:      0    1761    2217 
   Size:       1761     456
 
BanI G'GyrC_C

Cuts at:      0       3     636    2217 
   Size:          3     633    1581

  Fragments arranged by size: 

                1581     633       3
 
BanII G_rGCy'C

Cuts at:      0      47    2217 
   Size:         47    2170
 
BbeI G_GCGC'C

Cuts at:      0       7    2217 
   Size:          7    2210
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1242    2217 
   Size:       1242     975
 
BfrBI ATG'CAT

Cuts at:      0    1989    2217 
   Size:       1989     228
 
BglII A'GATC_T

Cuts at:      0     284    1224    2217 
   Size:        284     940     993

  Fragments arranged by size: 

                 993     940     284
 
Bme1580I G_kGCm'C

Cuts at:      0      47    2217 
   Size:         47    2170
 
BmgBI CAC'GTC

Cuts at:      0     234    2217 
   Size:        234    1983
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1046    2217 
   Size:       1046    1171
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     244     351    2217 
   Size:        244     107    1866

  Fragments arranged by size: 

                1866     244     107
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1375    2217 
   Size:       1375     842
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1084    2217 
   Size:       1084    1133
 
BsaBI GATnn'nnATC

Cuts at:      0    1517    2217 
   Size:       1517     700
 
BsaHI Gr'CG_yC

Cuts at:      0       4    2217 
   Size:          4    2213
 
BsaWI w'CCGG_w

Cuts at:      0     207    2217 
   Size:        207    2010
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     145     175    2217 
   Size:        145      30    2042

  Fragments arranged by size: 

                2042     145      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     153     156     159     162    2217 
   Size:        153       3       3       3    2055

  Fragments arranged by size: 

                2055     153       3       3       3
 
BseYI C'CCAG_C

Cuts at:      0      46    2217 
   Size:         46    2171
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1611    2217 
   Size:       1611     606
 
BsiHKAI G_wGCw'C

Cuts at:      0     233    2217 
   Size:        233    1984
 
Bsp1286I G_dGCh'C

Cuts at:      0      47     233    2217 
   Size:         47     186    1984

  Fragments arranged by size: 

                1984     186      47
 
BspHI T'CATG_A

Cuts at:      0    1862    2217 
   Size:       1862     355
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     624    1349    1717    2217 
   Size:        624     725     368     500

  Fragments arranged by size: 

                 725     624     500     368
 
BsrDI GCAATG_nn'

Cuts at:      0    1344    2217 
   Size:       1344     873
 
BsrFI r'CCGG_y

Cuts at:      0     207    2217 
   Size:        207    2010
 
BssSI C'ACGA_G

Cuts at:      0     247    2217 
   Size:        247    1970
 
BstBI TT'CG_AA

Cuts at:      0     300    1037    2217 
   Size:        300     737    1180

  Fragments arranged by size: 

                1180     737     300
 
BstYI r'GATC_y

Cuts at:      0     284    1224    1761    2217 
   Size:        284     940     537     456

  Fragments arranged by size: 

                 940     537     456     284
 
Bsu36I CC'TnA_GG

Cuts at:      0    1508    2217 
   Size:       1508     709
 
BtgI C'CryG_G

Cuts at:      0     176    2217 
   Size:        176    2041
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1504    2217 
   Size:       1504     713
 
DraI TTT'AAA

Cuts at:      0     396    2217 
   Size:        396    1821
 
EaeI y'GGCC_r

Cuts at:      0    1677    2217 
   Size:       1677     540
 
EarI CTCTTCn'nnn_

Cuts at:      0     771     827    1015    1079    1796    2217 
   Size:        771      56     188      64     717     421

  Fragments arranged by size: 

                 771     717     421     188      64      56
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     244     351    1346    1658    2126    2217 
   Size:        244     107     995     312     468      91

  Fragments arranged by size: 

                 995     468     312     244     107      91
 
EcoO109I rG'GnC_Cy

Cuts at:      0      43    1054    2217 
   Size:         43    1011    1163

  Fragments arranged by size: 

                1163    1011      43
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     577    2217 
   Size:        577    1640
 
HaeII r_GCGC'y

Cuts at:      0       7     921    2217 
   Size:          7     914    1296

  Fragments arranged by size: 

                1296     914       7
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     397     429     430     462     496     528    1140    1172 
   Size:        397      32       1      32      34      32     612      32

Cuts at:   1172    1209    1241    2164    2196    2217 
   Size:         37      32     923      32      21

  Fragments arranged by size: 

                 923     612     397      37      34      32      32      32
                  32      32      32      21       1
 
HindIII A'AGCT_T

Cuts at:      0    2159    2217 
   Size:       2159      58
 
KasI G'GCGC_C

Cuts at:      0       3    2217 
   Size:          3    2214
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1060    2217 
   Size:       1060    1157
 
MscI TGG'CCA

Cuts at:      0    1679    2217 
   Size:       1679     538
 
MslI CAynn'nnrTG

Cuts at:      0      20     500    2217 
   Size:         20     480    1717

  Fragments arranged by size: 

                1717     480      20
 
MspA1I CmG'CkG

Cuts at:      0     178     187    2217 
   Size:        178       9    2030

  Fragments arranged by size: 

                2030     178       9
 
NarI GG'CG_CC

Cuts at:      0       4    2217 
   Size:          4    2213
 
NdeI CA'TA_TG

Cuts at:      0     728    2217 
   Size:        728    1489
 
NsiI A_TGCA'T

Cuts at:      0    1991    2217 
   Size:       1991     226
 
NspI r_CATG'y

Cuts at:      0     354    1620    1989    2217 
   Size:        354    1266     369     228

  Fragments arranged by size: 

                1266     369     354     228
 
PciI A'CATG_T

Cuts at:      0     350    2217 
   Size:        350    1867
 
PfoI T'CCnGG_A

Cuts at:      0     768    2217 
   Size:        768    1449
 
PpuMI rG'GwC_Cy

Cuts at:      0    1054    2217 
   Size:       1054    1163
 
PspOMI G'GGCC_C

Cuts at:      0      43    2217 
   Size:         43    2174
 
SacII CC_GC'GG

Cuts at:      0     179    2217 
   Size:        179    2038
 
SapI GCTCTTCn'nnn_

Cuts at:      0     827    2217 
   Size:        827    1390
 
SfcI C'TryA_G

Cuts at:      0     288    1220    2217 
   Size:        288     932     997

  Fragments arranged by size: 

                 997     932     288
 
SfoI GGC'GCC

Cuts at:      0       5    2217 
   Size:          5    2212
 
SmlI C'TyrA_G

Cuts at:      0    1390    2217 
   Size:       1390     827
 
SphI G_CATG'C

Cuts at:      0    1989    2217 
   Size:       1989     228
 
SspI AAT'ATT

Cuts at:      0     326     819    1283    2217 
   Size:        326     493     464     934

  Fragments arranged by size: 

                 934     493     464     326
 
StuI AGG'CCT

Cuts at:      0     265    2217 
   Size:        265    1952
 
StyI C'CwwG_G

Cuts at:      0    1351    2217 
   Size:       1351     866
 
TatI w'GTAC_w

Cuts at:      0    1357    2217 
   Size:       1357     860
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     960    2217 
   Size:        960    1257
 
XmnI GAAnn'nnTTC

Cuts at:      0     836    2217 
   Size:        836    1381
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AgeI     AleI     ApaI     ApoI     AseI
     AvaI    BamHI     BanI    BanII     BbeI    BciVI    BfrBI    BglII
 Bme1580I    BmgBI     BplI     BpmI    BpuEI     BsaI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspHI
    BspMI    BsrDI    BsrFI    BssSI    BstBI    BstYI   Bsu36I     BtgI
    BtgZI     DraI     EaeI     EarI  Eco57MI EcoO109I     FalI    HaeII
    Hin4I  HindIII     KasI     MmeI     MscI     MslI   MspA1I     NarI
     NdeI     NsiI     NspI     PciI     PfoI    PpuMI   PspOMI    SacII
     SapI     SfcI     SfoI     SmlI     SphI     SspI     StuI     StyI
     TatI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AhdI     AlfI
     AloI    AlwNI    ApaLI     AscI    AsiSI    AvrII     BaeI     BbsI
    BbvCI     BcgI     BclI     BglI     BlpI     BmrI     BmtI   Bpu10I
    BsaAI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BsrBI    BsrGI
   BssHII   BstAPI   BstEII    BstXI  BstZ17I     BtsI     ClaI    CspCI
   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FseI     FspI    FspAI   HincII     HpaI     KpnI     MfeI     MluI
     NaeI     NcoI   NgoMIV     NheI     NotI     NruI     PacI     PasI
    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI     SalI    SanDI     SbfI
     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SrfI     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI     XmaI
     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com