Restriction map for pF1KF0188
 (Linear) (Six-base) MAPSORT of: FLJ00188.seq  Check: 3608  from: 1  to: 3831



FLJ00188.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:39  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    3177    3831 
   Size:       3177     654
 
AccI GT'mk_AC

Cuts at:      0    3061    3831 
   Size:       3061     770
 
Acc65I G'GTAC_C

Cuts at:      0     852    2261    3831 
   Size:        852    1409    1570

  Fragments arranged by size: 

                1570    1409     852
 
AclI AA'CG_TT

Cuts at:      0    2162    3831 
   Size:       2162    1669
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     370     802    1036    1832    1913    2153    2211    3831 
   Size:        370     432     234     796      81     240      58    1620

  Fragments arranged by size: 

                1620     796     432     370     240     234      81      58
            
 
AflII C'TTAA_G

Cuts at:      0    2814    3831 
   Size:       2814    1017
 
AleI CACnn'nnGTG

Cuts at:      0    2816    3010    3831 
   Size:       2816     194     821

  Fragments arranged by size: 

                2816     821     194
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     655    1306    3373    3557    3831 
   Size:        655     651    2067     184     274

  Fragments arranged by size: 

                2067     655     651     274     184
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2443    2475    3831 
   Size:       2443      32    1356

  Fragments arranged by size: 

                2443    1356      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1274    1543    2370    2403    2573    3696    3831 
   Size:       1274     269     827      33     170    1123     135

  Fragments arranged by size: 

                1274    1123     827     269     170     135      33
 
ApaI G_GGCC'C

Cuts at:      0    1023    1202    3831 
   Size:       1023     179    2629

  Fragments arranged by size: 

                2629    1023     179
 
ApoI r'AATT_y

Cuts at:      0     371    1488    2755    3590    3831 
   Size:        371    1117    1267     835     241

  Fragments arranged by size: 

                1267    1117     835     371     241
 
AvaI C'yCGr_G

Cuts at:      0      67     259     556    1438    2889    3831 
   Size:         67     192     297     882    1451     942

  Fragments arranged by size: 

                1451     942     882     297     192      67
 
AvrII C'CTAG_G

Cuts at:      0    1009    3831 
   Size:       1009    2822
 
BamHI G'GATC_C

Cuts at:      0     334    1557    3831 
   Size:        334    1223    2274

  Fragments arranged by size: 

                2274    1223     334
 
BanI G'GyrC_C

Cuts at:      0      27     250     511     690     852    1940    2052    2261 
   Size:         27     223     261     179     162    1088     112     209

Cuts at:   2261    2893    3668    3831 
   Size:        632     775     163

  Fragments arranged by size: 

                1088     775     632     261     223     209     179     163
                 162     112      27
 
BanII G_rGCy'C

Cuts at:      0     468     484     793     931    1023    1202    1420    1429 
   Size:        468      16     309     138      92     179     218       9

Cuts at:   1429    1438    1741    1932    2189    2909    3831 
   Size:          9     303     191     257     720     922

  Fragments arranged by size: 

                 922     720     468     309     303     257     218     191
                 179     138      92      16       9       9
 
BbeI G_GCGC'C

Cuts at:      0    1944    2897    3831 
   Size:       1944     953     934

  Fragments arranged by size: 

                1944     953     934
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     951    1820    1850    1901    2243    3521    3831 
   Size:        951     869      30      51     342    1278     310

  Fragments arranged by size: 

                1278     951     869     342     310      51      30
 
BbvCI CC'TCA_GC

Cuts at:      0    3439    3831 
   Size:       3439     392
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3513    3547    3831 
   Size:       3513      34     284

  Fragments arranged by size: 

                3513     284      34
 
BclI T'GATC_A

Cuts at:      0    2303    3134    3831 
   Size:       2303     831     697

  Fragments arranged by size: 

                2303     831     697
 
BfrBI ATG'CAT

Cuts at:      0    1140    3831 
   Size:       1140    2691
 
BglI GCCn_nnn'nGGC

Cuts at:      0     170    3831 
   Size:        170    3661
 
BglII A'GATC_T

Cuts at:      0    3020    3831 
   Size:       3020     811
 
BlpI GC'TnA_GC

Cuts at:      0    1182    3831 
   Size:       1182    2649
 
Bme1580I G_kGCm'C

Cuts at:      0      30     255     516     538     695    1023    1202    1479 
   Size:         30     225     261      22     157     328     179     277

Cuts at:   1479    3673    3831 
   Size:       2194     158

  Fragments arranged by size: 

                2194     328     277     261     225     179     158     157
                  30      22
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2380    2522    3831 
   Size:       2380     142    1309

  Fragments arranged by size: 

                2380    1309     142
 
BmtI G_CTAG'C

Cuts at:      0    2722    3831 
   Size:       2722    1109
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58     942    1014    1052    1080    1692    1892    2499 
   Size:         58     884      72      38      28     612     200     607

Cuts at:   2499    2923    3178    3831 
   Size:        424     255     653

  Fragments arranged by size: 

                 884     653     612     607     424     255     200      72
                  58      38      28
 
Bpu10I CC'TnA_GC

Cuts at:      0     489     868    1370    3439    3831 
   Size:        489     379     502    2069     392

  Fragments arranged by size: 

                2069     502     489     392     379
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2803    3040    3831 
   Size:       2803     237     791

  Fragments arranged by size: 

                2803     791     237
 
BsaBI GATnn'nnATC

Cuts at:      0    2980    3831 
   Size:       2980     851
 
BsaHI Gr'CG_yC

Cuts at:      0     113    1941    2136    2894    3831 
   Size:        113    1828     195     758     937

  Fragments arranged by size: 

                1828     937     758     195     113
 
BsaWI w'CCGG_w

Cuts at:      0     323    3824    3831 
   Size:        323    3501       7

  Fragments arranged by size: 

                3501     323       7
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     990    1020    1066    1096    3831 
   Size:        990      30      46      30    2735

  Fragments arranged by size: 

                2735     990      46      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     285     765     939    1011    1663    1743    2233    2548 
   Size:        285     480     174      72     652      80     490     315

Cuts at:   2548    2560    3831 
   Size:         12    1271

  Fragments arranged by size: 

                1271     652     490     480     315     285     174      80
                  72      12
 
BseYI C'CCAG_C

Cuts at:      0     204    3203    3743    3831 
   Size:        204    2999     540      88

  Fragments arranged by size: 

                2999     540     204      88
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     113    1143    1260    3034    3563    3682    3831 
   Size:        113    1030     117    1774     529     119     149

  Fragments arranged by size: 

                1774    1030     529     149     119     117     113
 
BsiHKAI G_wGCw'C

Cuts at:      0     589     763     793    1105    2110    2909    3831 
   Size:        589     174      30     312    1005     799     922

  Fragments arranged by size: 

                1005     922     799     589     312     174      30
 
BsmI GAATG_Cn'

Cuts at:      0    1908    3831 
   Size:       1908    1923
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1098    1740    3831 
   Size:       1098     642    2091

  Fragments arranged by size: 

                2091    1098     642
 
Bsp1286I G_dGCh'C

Cuts at:      0      30     255     468     484     516     538     589     695 
   Size:         30     225     213      16      32      22      51     106

Cuts at:    695     763     793     931    1023    1105    1202    1420    1429 
   Size:         68      30     138      92      82      97     218       9

Cuts at:   1429    1438    1479    1741    1932    2110    2189    2909    3673 
   Size:          9      41     262     191     178      79     720     764

Cuts at:   3673    3831 
   Size:        158

  Fragments arranged by size: 

                 764     720     262     225     218     213     191     178
                 158     138     106      97      92      82      79      68
                  51      41      32      30      30      22      16       9
                   9
 
BspEI T'CCGG_A

Cuts at:      0     323    3824    3831 
   Size:        323    3501       7

  Fragments arranged by size: 

                3501     323       7
 
BspHI T'CATG_A

Cuts at:      0    2918    3831 
   Size:       2918     913
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1165    2273    3177    3350    3741    3831 
   Size:       1165    1108     904     173     391      90

  Fragments arranged by size: 

                1165    1108     904     391     173      90
 
BsrBI CCG'CTC

Cuts at:      0     264     596    2631    3831 
   Size:        264     332    2035    1200

  Fragments arranged by size: 

                2035    1200     332     264
 
BsrDI GCAATG_nn'

Cuts at:      0     981    3831 
   Size:        981    2850
 
BsrFI r'CCGG_y

Cuts at:      0    1064    1624    2019    2138    3374    3831 
   Size:       1064     560     395     119    1236     457

  Fragments arranged by size: 

                1236    1064     560     457     395     119
 
BsrGI T'GTAC_A

Cuts at:      0    2636    3831 
   Size:       2636    1195
 
BssSI C'ACGA_G

Cuts at:      0     712    3831 
   Size:        712    3119
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     511    1450    2592    3831 
   Size:        511     939    1142    1239

  Fragments arranged by size: 

                1239    1142     939     511
 
BstEII G'GTnAC_C

Cuts at:      0     123    3831 
   Size:        123    3708
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     282     530     898    2121    3831 
   Size:        282     248     368    1223    1710

  Fragments arranged by size: 

                1710    1223     368     282     248
 
BstYI r'GATC_y

Cuts at:      0     334    1557    2827    3020    3831 
   Size:        334    1223    1270     193     811

  Fragments arranged by size: 

                1270    1223     811     334     193
 
BstZ17I GTA'TAC

Cuts at:      0    3062    3831 
   Size:       3062     769
 
Bsu36I CC'TnA_GG

Cuts at:      0     517     829    2650    3831 
   Size:        517     312    1821    1181

  Fragments arranged by size: 

                1821    1181     517     312
 
BtgI C'CryG_G

Cuts at:      0     275    1163    2354    3674    3831 
   Size:        275     888    1191    1320     157

  Fragments arranged by size: 

                1320    1191     888     275     157
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2967    3831 
   Size:       2967     864
 
BtsI GCAGTG_nn'

Cuts at:      0      50    1148    1217    2445    3831 
   Size:         50    1098      69    1228    1386

  Fragments arranged by size: 

                1386    1228    1098      69      50
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    2712    2747    3831 
   Size:       2712      35    1084

  Fragments arranged by size: 

                2712    1084      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    1579    2052    3831 
   Size:       1579     473    1779

  Fragments arranged by size: 

                1779    1579     473
 
EaeI y'GGCC_r

Cuts at:      0     684    1750    1762    1779    1787    2288    3044    3831 
   Size:        684    1066      12      17       8     501     756     787

  Fragments arranged by size: 

                1066     787     756     684     501      17      12       8
            
 
EarI CTCTTCn'nnn_

Cuts at:      0     235     781    1511    2492    3796    3831 
   Size:        235     546     730     981    1304      35

  Fragments arranged by size: 

                1304     981     730     546     235      35
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    3781    3831 
   Size:       3781      50
 
EcoICRI GAG'CTC

Cuts at:      0     791    2907    3831 
   Size:        791    2116     924

  Fragments arranged by size: 

                2116     924     791
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58     370     802     942    1014    1036    1052    1080 
   Size:         58     312     432     140      72      22      16      28

Cuts at:   1080    1692    1832    1892    1913    2153    2211    2499    2923 
   Size:        612     140      60      21     240      58     288     424

Cuts at:   2923    3178    3831 
   Size:        255     653

  Fragments arranged by size: 

                 653     612     432     424     312     288     255     240
                 140     140      72      60      58      58      28      22
                  21      16
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     996    2245    3807    3831 
   Size:        996    1249    1562      24

  Fragments arranged by size: 

                1562    1249     996      24
 
EcoO109I rG'GnC_Cy

Cuts at:      0      76     720     863    1019    1020    1198    1199    1540 
   Size:         76     644     143     156       1     178       1     341

Cuts at:   1540    1596    1860    3339    3693    3831 
   Size:         56     264    1479     354     138

  Fragments arranged by size: 

                1479     644     354     341     264     178     156     143
                 138      76      56       1       1
 
EcoRI G'AATT_C

Cuts at:      0     371    1488    3831 
   Size:        371    1117    2343

  Fragments arranged by size: 

                2343    1117     371
 
EcoRV GAT'ATC

Cuts at:      0    3219    3831 
   Size:       3219     612
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     810    1895    3831 
   Size:        810    1085    1936

  Fragments arranged by size: 

                1936    1085     810
 
FspI TGC'GCA

Cuts at:      0    2328    2714    3349    3831 
   Size:       2328     386     635     482

  Fragments arranged by size: 

                2328     635     482     386
 
HaeII r_GCGC'y

Cuts at:      0     455    1944    2897    3717    3831 
   Size:        455    1489     953     820     114

  Fragments arranged by size: 

                1489     953     820     455     114
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     990    1022    1066    1098    3203    3217    3235    3249 
   Size:        990      32      44      32    2105      14      18      14

Cuts at:   3249    3831 
   Size:        582

  Fragments arranged by size: 

                2105     990     582      44      32      32      18      14
                  14
 
HincII GTy'rAC

Cuts at:      0    1358    3831 
   Size:       1358    2473
 
HindIII A'AGCT_T

Cuts at:      0     211     895    3831 
   Size:        211     684    2936

  Fragments arranged by size: 

                2936     684     211
 
KasI G'GCGC_C

Cuts at:      0    1940    2893    3831 
   Size:       1940     953     938

  Fragments arranged by size: 

                1940     953     938
 
KpnI G_GTAC'C

Cuts at:      0     856    2265    3831 
   Size:        856    1409    1566

  Fragments arranged by size: 

                1566    1409     856
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     217     575    2102    2183    2438    2486    2700    3831 
   Size:        217     358    1527      81     255      48     214    1131

  Fragments arranged by size: 

                1527    1131     358     255     217     214      81      48
            
 
MscI TGG'CCA

Cuts at:      0     686    1789    2290    3831 
   Size:        686    1103     501    1541

  Fragments arranged by size: 

                1541    1103     686     501
 
MslI CAynn'nnrTG

Cuts at:      0    1580    1615    1951    2698    2816    3010    3724    3831 
   Size:       1580      35     336     747     118     194     714     107

  Fragments arranged by size: 

                1580     747     714     336     194     118     107      35
            
 
MspA1I CmG'CkG

Cuts at:      0     582    1457    1785    2286    3036    3042    3294    3321 
   Size:        582     875     328     501     750       6     252      27

Cuts at:   3321    3831 
   Size:        510

  Fragments arranged by size: 

                 875     750     582     510     501     328     252      27
                   6
 
NaeI GCC'GGC

Cuts at:      0    1066    1626    2140    3376    3831 
   Size:       1066     560     514    1236     455

  Fragments arranged by size: 

                1236    1066     560     514     455
 
NarI GG'CG_CC

Cuts at:      0    1941    2894    3831 
   Size:       1941     953     937

  Fragments arranged by size: 

                1941     953     937
 
NcoI C'CATG_G

Cuts at:      0     275    2354    3831 
   Size:        275    2079    1477

  Fragments arranged by size: 

                2079    1477     275
 
NdeI CA'TA_TG

Cuts at:      0    3105    3831 
   Size:       3105     726
 
NgoMIV G'CCGG_C

Cuts at:      0    1064    1624    2138    3374    3831 
   Size:       1064     560     514    1236     457

  Fragments arranged by size: 

                1236    1064     560     514     457
 
NheI G'CTAG_C

Cuts at:      0    2718    3831 
   Size:       2718    1113
 
NsiI A_TGCA'T

Cuts at:      0    1142    3831 
   Size:       1142    2689
 
NspI r_CATG'y

Cuts at:      0     509    3360    3831 
   Size:        509    2851     471

  Fragments arranged by size: 

                2851     509     471
 
PasI CC'CwG_GG

Cuts at:      0      23    3831 
   Size:         23    3808
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     276     508     920    1030    1345    2370    2403    3321 
   Size:        276     232     412     110     315    1025      33     918

Cuts at:   3321    3831 
   Size:        510

  Fragments arranged by size: 

                1025     918     510     412     315     276     232     110
                  33
 
PfoI T'CCnGG_A

Cuts at:      0    1096    1307    2609    3831 
   Size:       1096     211    1302    1222

  Fragments arranged by size: 

                1302    1222    1096     211
 
PpuMI rG'GwC_Cy

Cuts at:      0     720     863    1596    1860    3339    3831 
   Size:        720     143     733     264    1479     492

  Fragments arranged by size: 

                1479     733     720     492     264     143
 
PshAI GACnn'nnGTC

Cuts at:      0    2602    3831 
   Size:       2602    1229
 
PspOMI G'GGCC_C

Cuts at:      0    1019    1198    3831 
   Size:       1019     179    2633

  Fragments arranged by size: 

                2633    1019     179
 
PspXI vC'TCGA_Gb

Cuts at:      0     556    3831 
   Size:        556    3275
 
PstI C_TGCA'G

Cuts at:      0     163    1830    2270    2969    3041    3536    3831 
   Size:        163    1667     440     699      72     495     295

  Fragments arranged by size: 

                1667     699     495     440     295     163      72
 
PvuII CAG'CTG

Cuts at:      0    1457    1785    2286    3042    3321    3831 
   Size:       1457     328     501     756     279     510

  Fragments arranged by size: 

                1457     756     510     501     328     279
 
SacI G_AGCT'C

Cuts at:      0     793    2909    3831 
   Size:        793    2116     922

  Fragments arranged by size: 

                2116     922     793
 
SanDI GG'GwC_CC

Cuts at:      0     720    3831 
   Size:        720    3111
 
SapI GCTCTTCn'nnn_

Cuts at:      0     781    1511    2492    3796    3831 
   Size:        781     730     981    1304      35

  Fragments arranged by size: 

                1304     981     781     730      35
 
ScaI AGT'ACT

Cuts at:      0    2875    3831 
   Size:       2875     956
 
SexAI A'CCwGG_T

Cuts at:      0    2048    3831 
   Size:       2048    1783
 
SfcI C'TryA_G

Cuts at:      0     159     744    1826    2266    2965    3037    3532    3831 
   Size:        159     585    1082     440     699      72     495     299

  Fragments arranged by size: 

                1082     699     585     495     440     299     159      72
            
 
SfoI GGC'GCC

Cuts at:      0    1942    2895    3831 
   Size:       1942     953     936

  Fragments arranged by size: 

                1942     953     936
 
SgrAI Cr'CCGG_yG

Cuts at:      0    2138    3831 
   Size:       2138    1693
 
SmaI CCC'GGG

Cuts at:      0    1440    2891    3831 
   Size:       1440    1451     940

  Fragments arranged by size: 

                1451    1440     940
 
SmlI C'TyrA_G

Cuts at:      0     556    2782    2814    3055    3831 
   Size:        556    2226      32     241     776

  Fragments arranged by size: 

                2226     776     556     241      32
 
StuI AGG'CCT

Cuts at:      0    1758    3538    3831 
   Size:       1758    1780     293

  Fragments arranged by size: 

                1780    1758     293
 
StyI C'CwwG_G

Cuts at:      0     275     941    1009    1753    2354    3831 
   Size:        275     666      68     744     601    1477

  Fragments arranged by size: 

                1477     744     666     601     275      68
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     199     684    1419    3242    3831 
   Size:        199     485     735    1823     589

  Fragments arranged by size: 

                1823     735     589     485     199
 
TatI w'GTAC_w

Cuts at:      0    2636    2792    2873    2933    3362    3831 
   Size:       2636     156      81      60     429     469

  Fragments arranged by size: 

                2636     469     429     156      81      60
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2413    2445    3831 
   Size:       2413      32    1386

  Fragments arranged by size: 

                2413    1386      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     955    3831 
   Size:        955    2876
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     282    3120    3831 
   Size:        282    2838     711

  Fragments arranged by size: 

                2838     711     282
 
XhoI C'TCGA_G

Cuts at:      0     556    3831 
   Size:        556    3275
 
XmaI C'CCGG_G

Cuts at:      0    1438    2889    3831 
   Size:       1438    1451     942

  Fragments arranged by size: 

                1451    1438     942
 
XmnI GAAnn'nnTTC

Cuts at:      0    3242    3831 
   Size:       3242     589
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI    AflII     AleI     AlfI
     AloI    AlwNI     ApaI     ApoI     AvaI    AvrII    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I     BmrI     BmtI     BpmI   Bpu10I    BpuEI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI    BssSI   BstAPI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI    CspCI   DraIII     EaeI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FspI    HaeII    Hin4I   HincII  HindIII     KasI     KpnI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NsiI     NspI     PasI    PflMI     PfoI    PpuMI    PshAI
   PspOMI    PspXI     PstI    PvuII     SacI    SanDI     SapI     ScaI
    SexAI     SfcI     SfoI    SgrAI     SmaI     SmlI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AfeI   AflIII     AgeI     AhdI    ApaLI     AscI     AseI
    AsiSI     BaeI    BciVI    BmgBI     BplI     BsaI    BsaAI    BsiEI
    BsiWI   BssHII    BstBI     ClaI     DraI     DrdI     EagI     FseI
    FspAI     HpaI     MfeI     MluI     NotI     NruI     PacI     PciI
     PmeI     PmlI     PpiI     PsiI     PsrI     PvuI    RsrII    SacII
     SalI     SbfI     SfiI     SgfI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI     XbaI     ZraI
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