Restriction map for pF1KSDB3041
##################################
# NO CUT ENZYMES (   95 enzymes) #
##################################

AatII       AccII       AclI        AcyI        AflII       
AgeI        AhdI        Alw44I      ApaI        ApaLI       
AscI        AspEI       AspI        AviII       BbeI        
BbrPI       BbvCI       BclI        BfrI        BglII       
BsaAI       BsaBI       BsaHI       BsiEI       BsiWI       
Bsp1407I    BspDI       BspHI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      Bst1107I    BstBI       
BstUI       BstZ17I     Cfr10I      ClaI        CpoI        
EagI        Eam1105I    EclXI       Eco52I      EcoRV       
EcoT22I     FbaI        FseI        FspI        Hin1I       
HindIII     HpaI        KasI        KspI        MamI        
MluI        MvnI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PflFI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SalI        
SbfI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        Sse8387I    
SspBI       SwaI        Tth111I     XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

Acc65I       1111  GGTACC               G^GTACC
AccI          715  GTAGAC               GT^[AC][GT]AC
AccIII       2384  TCCGGA               T^CCGGA
AflIII       3582  ACATGT               A^C[GA][CT]GT
Aor51HI      1593  AGCGCT               AGC^GCT
AseI          781  ATTAAT               AT^TAAT
AsnI          781  ATTAAT               AT^TAAT
Asp700I      1729  GAATTGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1111  GGTACC               G^GTACC
AvaI         2222  CTCGAG               C^[CT]CG[GA]G
AvrII         346  CCTAGG               C^CTAGG
BalI         3641  TGGCCA               TGG^CCA
BanI         1111  GGTACC               G^G[CT][GA]CC
BcgI         3113  GCATGGACATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI          106  CCCGG                CC^[GC]GG
BlnI          346  CCTAGG               C^CTAGG
BsaWI        2384  TCCGGA               [AT]^CCGG[AT]
BseAI        2384  TCCGGA               T^CCGGA
BsgI         3294  GTGCAG               GTGCAG(16/14)
BsmI         3147  GCATTC               GAATGC(1/-1)
BsoBI        2222  CTCGAG               C^[CT]CG[GA]G
BspEI        2384  TCCGGA               T^CCGGA
BspLU11I     3582  ACATGT               A^CATGT
BstEII       3441  GGTGACC              G^GT[ACGT]ACC
BstPI        3441  GGTGACC              G^GT[ACGT]ACC
BstXI        2573  CCACCATTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI          229  TTTAAA               TTT^AAA
DraII        1610  AGGCCCT              [GA]G^G[ACGT]CC[CT]
DraIII       3342  CACAAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1509  GACGAGCAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI         3641  TGGCCA               [CT]^GGCC[GA]
Eco47III     1593  AGCGCT               AGC^GCT
EcoO109I     1610  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I      3441  GGTGACC              G^GT[ACGT]ACC
FauI         1938  GCGGG                CCCGC(4/6)
HincII        876  GTTGAC               GT[CT]^[GA]AC
HindII        876  GTTGAC               GT[CT]^[GA]AC
KpnI         1111  GGTACC               GGTAC^C
MfeI         1411  CAATTG               C^AATTG
MluNI        3641  TGGCCA               TGG^CCA
MroI         2384  TCCGGA               T^CCGGA
MscI         3641  TGGCCA               TGG^CCA
MunI         1411  CAATTG               C^AATTG
NciI          106  CCCGG                CC^[GC]GG
NcoI         1104  CCATGG               C^CATGG
PaeR7I       2222  CTCGAG               C^TCGAG
PshBI         781  ATTAAT               AT^TAAT
SacI         1306  GAGCTC               GAGCT^C
ScaI         2266  AGTACT               AGT^ACT
StuI          795  AGGCCT               AGG^CCT
XbaI          957  TCTAGA               T^CTAGA
XcmI         2548  CCAAAACAACGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         2222  CTCGAG               C^TCGAG
XmnI         1729  GAATTGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

BamHI         495  GGATCC               G^GATCC
             3429  GGATCC              
BanII        1306  GAGCTC               G[GA]GC[CT]^C
             3635  GGGCTC              
BciVI         555  GGATAC               GTATCC(6/5)
             3024  GGATAC              
BglI          601  GCCTCCACGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             3359  GCCAGCAAGGC         
BlpI         2752  GCTGAGC              GC^T[ACGT]AGC
             2877  GCTGAGC             
Bpu1102I     2752  GCTGAGC              GC^T[ACGT]AGC
             2877  GCTGAGC             
BsaI         2033  GAGACC               GGTCTC(1/5)
             2622  GGTCTC              
BssSI         464  CTCGTG               CACGAG(-5/-1)
             3083  CTCGTG              
CelII        2752  GCTGAGC              GC^T[ACGT]AGC
             2877  GCTGAGC             
EcoRI          20  GAATTC               G^AATTC
             2482  GAATTC              
HapII         107  CCGG                 C^CGG
             2385  CCGG                
HpaII         107  CCGG                 C^CGG
             2385  CCGG                
MaeII        1800  ACGT                 A^CGT
             2103  ACGT                
MspI          107  CCGG                 C^CGG
             2385  CCGG                
SapI         1303  GAAGAGC              GCTCTTC(1/4)
              363  GCTCTTC             
SphI         1317  GCATGC               GCATG^C
             1542  GCATGC              


#########################################
# 3 OR MORE CUT ENZYMES (   93 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       AocI        ApoI        AspHI       AvaII       
BbsI        BbvI        BfaI        BmyI        BpmI        
BpuAI       BsaJI       BseRI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmBI       BsmFI       Bsp1286I    
BspMI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstYI       Bsu36I      Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DsaI        EarI        
Eco81I      EcoNI       EcoRII      EcoT14I     Fnu4HI      
FokI        HaeII       HaeIII      HgaI        HhaI        
HinP1I      HinfI       HphI        ItaI        Ksp632I     
MaeI        MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MvaI        
MwoI        NdeII       NlaIII      NlaIV       NspI        
PflMI       PleI        PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SspI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XhoII       
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