Restriction map for pF1KSDB3041
 (Linear) (Six-base) MAPSORT of: KIBB3041.seq  Check: 1872  from: 1  to: 3696



KIBB3041.orf

 With 182 enzymes: SgfI * 

                        January 7, 2013 14:39  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    3513    3696 
   Size:       3513     183
 
AccI GT'mk_AC

Cuts at:      0     716    3696 
   Size:        716    2980
 
Acc65I G'GTAC_C

Cuts at:      0    1111    3696 
   Size:       1111    2585
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     776    1729    1855    2429    2908    3696 
   Size:        776     953     126     574     479     788

  Fragments arranged by size: 

                 953     788     776     574     479     126
 
AfeI AGC'GCT

Cuts at:      0    1595    3696 
   Size:       1595    2101
 
AflIII A'CryG_T

Cuts at:      0    3582    3696 
   Size:       3582     114
 
AleI CACnn'nnGTG

Cuts at:      0    2889    3696 
   Size:       2889     807
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1182    1812    2125    2520    2874    2877    2979    3528 
   Size:       1182     630     313     395     354       3     102     549

Cuts at:   3528    3696 
   Size:        168

  Fragments arranged by size: 

                1182     630     549     395     354     313     168     102
                   3
 
ApoI r'AATT_y

Cuts at:      0      20     397     440    2482    3696 
   Size:         20     377      43    2042    1214

  Fragments arranged by size: 

                2042    1214     377      43      20
 
AseI AT'TA_AT

Cuts at:      0     782    3696 
   Size:        782    2914
 
AvaI C'yCGr_G

Cuts at:      0    2222    3696 
   Size:       2222    1474
 
AvrII C'CTAG_G

Cuts at:      0     346    3696 
   Size:        346    3350
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1457    1490    3696 
   Size:       1457      33    2206

  Fragments arranged by size: 

                2206    1457      33
 
BamHI G'GATC_C

Cuts at:      0     495    3429    3696 
   Size:        495    2934     267

  Fragments arranged by size: 

                2934     495     267
 
BanI G'GyrC_C

Cuts at:      0    1111    3696 
   Size:       1111    2585
 
BanII G_rGCy'C

Cuts at:      0    1310    3639    3696 
   Size:       1310    2329      57

  Fragments arranged by size: 

                2329    1310      57
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     183     541    1370    1511    2546    3544    3696 
   Size:        183     358     829     141    1035     998     152

  Fragments arranged by size: 

                1035     998     829     358     183     152     141
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3102    3136    3696 
   Size:       3102      34     560

  Fragments arranged by size: 

                3102     560      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     549    3018    3696 
   Size:        549    2469     678

  Fragments arranged by size: 

                2469     678     549
 
BglI GCCn_nnn'nGGC

Cuts at:      0     607    3365    3696 
   Size:        607    2758     331

  Fragments arranged by size: 

                2758     607     331
 
BlpI GC'TnA_GC

Cuts at:      0    2753    2878    3696 
   Size:       2753     125     818

  Fragments arranged by size: 

                2753     818     125
 
Bme1580I G_kGCm'C

Cuts at:      0     107     860    2598    3385    3696 
   Size:        107     753    1738     787     311

  Fragments arranged by size: 

                1738     787     753     311     107
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     615    1416    3696 
   Size:        615     801    2280

  Fragments arranged by size: 

                2280     801     615
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2640    3696 
   Size:       2640    1056
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1789    1834    2137    2974    3023    3124    3445    3599 
   Size:       1789      45     303     837      49     101     321     154

Cuts at:   3599    3696 
   Size:         97

  Fragments arranged by size: 

                1789     837     321     303     154     101      97      49
                  45
 
Bpu10I CC'TnA_GC

Cuts at:      0    1309    3068    3696 
   Size:       1309    1759     628

  Fragments arranged by size: 

                1759    1309     628
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2125    2309    2929    3696 
   Size:       2125     184     620     767

  Fragments arranged by size: 

                2125     767     620     184
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2027    2628    3696 
   Size:       2027     601    1068

  Fragments arranged by size: 

                2027    1068     601
 
BsaWI w'CCGG_w

Cuts at:      0    2384    3696 
   Size:       2384    1312
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2606    2636    2969    2999    3696 
   Size:       2606      30     333      30     697

  Fragments arranged by size: 

                2606     697     333      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1204    2128    2616    2977    3696 
   Size:       1204     924     488     361     719

  Fragments arranged by size: 

                1204     924     719     488     361
 
BseYI C'CCAG_C

Cuts at:      0    2558    3546    3696 
   Size:       2558     988     150

  Fragments arranged by size: 

                2558     988     150
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    3315    3696 
   Size:       3315     381
 
BsiHKAI G_wGCw'C

Cuts at:      0     828    1310    2099    2496    2884    3696 
   Size:        828     482     789     397     388     812

  Fragments arranged by size: 

                 828     812     789     482     397     388
 
BsmI GAATG_Cn'

Cuts at:      0    3147    3696 
   Size:       3147     549
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1501    1807    2143    3696 
   Size:       1501     306     336    1553

  Fragments arranged by size: 

                1553    1501     336     306
 
Bsp1286I G_dGCh'C

Cuts at:      0     107     828     860    1310    2099    2496    2598    2884 
   Size:        107     721      32     450     789     397     102     286

Cuts at:   2884    3385    3639    3696 
   Size:        501     254      57

  Fragments arranged by size: 

                 789     721     501     450     397     286     254     107
                 102      57      32
 
BspEI T'CCGG_A

Cuts at:      0    2384    3696 
   Size:       2384    1312
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1412    1505    3513    3696 
   Size:       1412      93    2008     183

  Fragments arranged by size: 

                2008    1412     183      93
 
BssSI C'ACGA_G

Cuts at:      0     464    3083    3696 
   Size:        464    2619     613

  Fragments arranged by size: 

                2619     613     464
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     307    2125    2520    2700    3696 
   Size:        307    1818     395     180     996

  Fragments arranged by size: 

                1818     996     395     307     180
 
BstEII G'GTnAC_C

Cuts at:      0    3441    3696 
   Size:       3441     255
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2580    3696 
   Size:       2580    1116
 
BstYI r'GATC_y

Cuts at:      0     447     495    1123    1335    2315    3126    3429    3494 
   Size:        447      48     628     212     980     811     303      65

Cuts at:   3494    3696 
   Size:        202

  Fragments arranged by size: 

                 980     811     628     447     303     212     202      65
                  48
 
Bsu36I CC'TnA_GG

Cuts at:      0     116     500    3020    3696 
   Size:        116     384    2520     676

  Fragments arranged by size: 

                2520     676     384     116
 
BtgI C'CryG_G

Cuts at:      0     605    1104    2199    3696 
   Size:        605     499    1095    1497

  Fragments arranged by size: 

                1497    1095     605     499
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    3527    3696 
   Size:       3527     169
 
BtsI GCAGTG_nn'

Cuts at:      0    2213    3292    3696 
   Size:       2213    1079     404

  Fragments arranged by size: 

                2213    1079     404
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     828     863    3270    3305    3696 
   Size:        828      35    2407      35     391

  Fragments arranged by size: 

                2407     828     391      35      35
 
DraI TTT'AAA

Cuts at:      0     231    3696 
   Size:        231    3465
 
DraIII CAC_nnn'GTG

Cuts at:      0    3347    3696 
   Size:       3347     349
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1515    3696 
   Size:       1515    2181
 
EaeI y'GGCC_r

Cuts at:      0    3641    3696 
   Size:       3641      55
 
EarI CTCTTCn'nnn_

Cuts at:      0       2     370    1298    3051    3680    3696 
   Size:          2     368     928    1753     629      16

  Fragments arranged by size: 

                1753     928     629     368      16       2
 
EcoICRI GAG'CTC

Cuts at:      0    1308    3696 
   Size:       1308    2388
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     776    1729    1789    1834    1855    2137    2429    2908 
   Size:        776     953      60      45      21     282     292     479

Cuts at:   2908    2974    3023    3124    3445    3599    3696 
   Size:         66      49     101     321     154      97

  Fragments arranged by size: 

                 953     776     479     321     292     282     154     101
                  97      66      60      49      45      21
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     503    1186    2989    3696 
   Size:        503     683    1803     707

  Fragments arranged by size: 

                1803     707     683     503
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1611    3696 
   Size:       1611    2085
 
EcoRI G'AATT_C

Cuts at:      0      20    2482    3696 
   Size:         20    2462    1214

  Fragments arranged by size: 

                2462    1214      20
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     408     867    3393    3696 
   Size:        408     459    2526     303

  Fragments arranged by size: 

                2526     459     408     303
 
HaeII r_GCGC'y

Cuts at:      0      40     211    1597    1791    3696 
   Size:         40     171    1386     194    1905

  Fragments arranged by size: 

                1905    1386     194     171      40
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    2307    2339    2606    2638    3696 
   Size:       2307      32     267      32    1058

  Fragments arranged by size: 

                2307    1058     267      32      32
 
HincII GTy'rAC

Cuts at:      0     878    3696 
   Size:        878    2818
 
KpnI G_GTAC'C

Cuts at:      0    1115    3696 
   Size:       1115    2581
 
MfeI C'AATT_G

Cuts at:      0    1411    3696 
   Size:       1411    2285
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1229    3331    3696 
   Size:       1229    2102     365

  Fragments arranged by size: 

                2102    1229     365
 
MscI TGG'CCA

Cuts at:      0    3643    3696 
   Size:       3643      53
 
MslI CAynn'nnrTG

Cuts at:      0     610    2291    2889    3167    3696 
   Size:        610    1681     598     278     529

  Fragments arranged by size: 

                1681     610     598     529     278
 
MspA1I CmG'CkG

Cuts at:      0    1045    1228    1938    2125    2681    2811    2874    3696 
   Size:       1045     183     710     187     556     130      63     822

  Fragments arranged by size: 

                1045     822     710     556     187     183     130      63
            
 
NcoI C'CATG_G

Cuts at:      0    1104    3696 
   Size:       1104    2592
 
NspI r_CATG'y

Cuts at:      0    1321    1546    2694    3586    3696 
   Size:       1321     225    1148     892     110

  Fragments arranged by size: 

                1321    1148     892     225     110
 
PciI A'CATG_T

Cuts at:      0    3582    3696 
   Size:       3582     114
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     334    1248    1812    3696 
   Size:        334     914     564    1884

  Fragments arranged by size: 

                1884     914     564     334
 
PfoI T'CCnGG_A

Cuts at:      0    1805    3101    3235    3696 
   Size:       1805    1296     134     461

  Fragments arranged by size: 

                1805    1296     461     134
 
PspXI vC'TCGA_Gb

Cuts at:      0    2222    3696 
   Size:       2222    1474
 
PstI C_TGCA'G

Cuts at:      0     312    1409    1458    1652    2220    2816    3696 
   Size:        312    1097      49     194     568     596     880

  Fragments arranged by size: 

                1097     880     596     568     312     194      49
 
PvuII CAG'CTG

Cuts at:      0    1045    1228    2125    2681    2811    2874    3696 
   Size:       1045     183     897     556     130      63     822

  Fragments arranged by size: 

                1045     897     822     556     183     130      63
 
SacI G_AGCT'C

Cuts at:      0    1310    3696 
   Size:       1310    2386
 
SapI GCTCTTCn'nnn_

Cuts at:      0     370    1298    3696 
   Size:        370     928    2398

  Fragments arranged by size: 

                2398     928     370
 
ScaI AGT'ACT

Cuts at:      0    2268    3696 
   Size:       2268    1428
 
SfcI C'TryA_G

Cuts at:      0     308     861    1096    1405    1454    1648    2216    2509 
   Size:        308     553     235     309      49     194     568     293

Cuts at:   2509    2812    3696 
   Size:        303     884

  Fragments arranged by size: 

                 884     568     553     309     308     303     293     235
                 194      49
 
SmlI C'TyrA_G

Cuts at:      0    2140    2222    2324    2908    3696 
   Size:       2140      82     102     584     788

  Fragments arranged by size: 

                2140     788     584     102      82
 
SphI G_CATG'C

Cuts at:      0    1321    1546    3696 
   Size:       1321     225    2150

  Fragments arranged by size: 

                2150    1321     225
 
SspI AAT'ATT

Cuts at:      0     786    1029    2568    2605    3136    3696 
   Size:        786     243    1539      37     531     560

  Fragments arranged by size: 

                1539     786     560     531     243      37
 
StuI AGG'CCT

Cuts at:      0     797    3696 
   Size:        797    2899
 
StyI C'CwwG_G

Cuts at:      0     346     551     749    1104    3249    3696 
   Size:        346     205     198     355    2145     447

  Fragments arranged by size: 

                2145     447     355     346     205     198
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    3281    3696 
   Size:       3281     415
 
TatI w'GTAC_w

Cuts at:      0    2266    3696 
   Size:       2266    1430
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2575    2607    3696 
   Size:       2575      32    1089

  Fragments arranged by size: 

                2575    1089      32
 
XbaI T'CTAG_A

Cuts at:      0     957    3696 
   Size:        957    2739
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2555    3696 
   Size:       2555    1141
 
XhoI C'TCGA_G

Cuts at:      0    2222    3696 
   Size:       2222    1474
 
XmnI GAAnn'nnTTC

Cuts at:      0    1733    3696 
   Size:       1733    1963
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AfeI   AflIII     AleI    AlwNI
     ApoI     AseI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbsI     BcgI    BciVI     BglI     BlpI Bme1580I     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaWI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspMI    BssSI
   BstAPI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
    CspCI     DraI   DraIII     DrdI     EaeI     EarI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI     FalI    HaeII    Hin4I   HincII     KpnI
     MfeI     MmeI     MscI     MslI   MspA1I     NcoI     NspI     PciI
    PflMI     PfoI    PspXI     PstI    PvuII     SacI     SapI     ScaI
     SfcI     SmlI     SphI     SspI     StuI     StyI    TaqII     TatI
     TstI     XbaI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII     AclI    AflII     AgeI     AhdI     AlfI     AloI     ApaI
    ApaLI     AscI    AsiSI     BbeI    BbvCI     BclI    BfrBI    BglII
    BmgBI     BmtI    BsaAI    BsaBI    BsaHI    BsiEI    BsiWI    BspHI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BstBI  BstZ17I     ClaI
     EagI     EciI    EcoRV     FseI     FspI    FspAI  HindIII     HpaI
     KasI     MluI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI     PsrI     PvuI    RsrII    SacII     SalI
    SanDI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SrfI     SwaI  Tth111I     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com