Restriction map for pF1KSDB1439
##################################
# NO CUT ENZYMES (   50 enzymes) #
##################################

AclI        AflII       AgeI        Alw44I      ApaLI       
AscI        AseI        AsnI        AspI        AvrII       
BfrI        BlnI        BsiWI       BssHII      Bst1107I    
BstBI       BstZ17I     CpoI        DraI        FseI        
HindIII     HpaI        KspI        MfeI        MluI        
MunI        NheI        NotI        NruI        NspV        
PacI        PflFI       PinAI       PmeI        PshBI       
Psp1406I    PvuI        RsrII       SacI        SacII       
SfiI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SwaI        Tth111I     XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AatII        4294  GACGTC               GACGT^C
Acc65I       2564  GGTACC               G^GTACC
AccII         658  CGCG                 CG^CG
AhdI         6756  GACTGAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI       632  AGCGCT               AGC^GCT
Asp700I      5272  GAAAACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      2564  GGTACC               G^GTACC
AspEI        6756  GACTGAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        3479  TGCGCA               TGC^GCA
BsiEI         868  CGGCCG               CG[GA][CT]^CG
Bsp1407I     1196  TGTACA               T^GTACA
BspDI        6067  ATCGAT               AT^CGAT
BsrGI        1196  TGTACA               T^GTACA
BssSI          85  CTCGTG               CACGAG(-5/-1)
BstUI         658  CGCG                 CG^CG
ClaI         6067  ATCGAT               AT^CGAT
DraIII       3555  CACCACGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI          868  CGGCCG               C^GGCCG
Eam1105I     6756  GACTGAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI         868  CGGCCG               C^GGCCG
Eco47III      632  AGCGCT               AGC^GCT
Eco52I        868  CGGCCG               C^GGCCG
EcoRV         463  GATATC               GAT^ATC
FspI         3479  TGCGCA               TGC^GCA
KpnI         2564  GGTACC               GGTAC^C
MvnI          658  CGCG                 CG^CG
NcoI         5642  CCATGG               C^CATGG
NdeI         3725  CATATG               CA^TATG
SalI         6803  GTCGAC               G^TCGAC
SbfI         4460  CCTGCAGG             CCTGCA^GG
ScaI         6698  AGTACT               AGT^ACT
Sse8387I     4460  CCTGCAGG             CCTGCA^GG
SspBI        1196  TGTACA               T^GTACA
XbaI         4824  TCTAGA               T^CTAGA
XmnI         5272  GAAAACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AccIII        416  TCCGGA               T^CCGGA
             4350  TCCGGA              
AlwNI        1123  CAGAGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             4066  CAGGCGCTG           
AocI         4930  CCTAAGG              CC^T[ACGT]AGG
             5975  CCTCAGG             
BamHI        1068  GGATCC               G^GATCC
             2715  GGATCC              
BbeI         3969  GGCGCC               GGCGC^C
             6436  GGCGCC              
BbrPI        1696  CACGTG               CAC^GTG
             3558  CACGTG              
BbvCI         185  CCTCAGC              CCTCAGC(-5/-2)
             5484  GCTGAGG             
BglII         811  AGATCT               A^GATCT
             1310  AGATCT              
BseAI         416  TCCGGA               T^CCGGA
             4350  TCCGGA              
BspEI         416  TCCGGA               T^CCGGA
             4350  TCCGGA              
BspLU11I     1634  ACATGT               A^CATGT
             1664  ACATGT              
BsrFI        3972  GCCGGC               [GA]^CCGG[CT]
             5926  GCCGGC              
BstEII        894  GGTGACC              G^GT[ACGT]ACC
             5172  GGTGACC             
BstPI         894  GGTGACC              G^GT[ACGT]ACC
             5172  GGTGACC             
Bsu36I       4930  CCTAAGG              CC^T[ACGT]AGG
             5975  CCTCAGG             
Cfr10I       3972  GCCGGC               [GA]^CCGG[CT]
             5926  GCCGGC              
DrdI          523  GACTCAGTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3946  GACTCCAATGTC        
Eco81I       4930  CCTAAGG              CC^T[ACGT]AGG
             5975  CCTCAGG             
EcoO65I       894  GGTGACC              G^GT[ACGT]ACC
             5172  GGTGACC             
EcoRI         848  GAATTC               G^AATTC
             2497  GAATTC              
EcoT22I      1112  ATGCAT               ATGCA^T
             2158  ATGCAT              
KasI         3969  GGCGCC               G^GCGCC
             6436  GGCGCC              
MroI          416  TCCGGA               T^CCGGA
             4350  TCCGGA              
NaeI         3972  GCCGGC               GCC^GGC
             5926  GCCGGC              
NarI         3969  GGCGCC               GG^CGCC
             6436  GGCGCC              
NgoMIV       3972  GCCGGC               G^CCGGC
             5926  GCCGGC              
NsiI         1112  ATGCAT               ATGCA^T
             2158  ATGCAT              
PaeR7I       3285  CTCGAG               C^TCGAG
             6785  CTCGAG              
PmaCI        1696  CACGTG               CAC^GTG
             3558  CACGTG              
PmlI         1696  CACGTG               CAC^GTG
             3558  CACGTG              
SfoI         3969  GGCGCC               GGC^GCC
             6436  GGCGCC              
SspI          825  AATATT               AAT^ATT
             6016  AATATT              
XhoI         3285  CTCGAG               C^TCGAG
             6785  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  140 enzymes) #
#########################################

AccI        AciI        AcsI        AcyI        AfaI        
AflIII      AluI        AlwI        ApaI        ApoI        
AspHI       AvaI        AvaII       BalI        BanI        
BanII       BbsI        BbvI        BcgI        BciVI       
BclI        BcnI        BfaI        BglI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaJI       BsaWI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1286I    BspHI       BspMI       BsrBI       BsrDI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstXI       BstYI       Cac8I       CelII       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        FbaI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MamI        MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PflMI       PleI        PpuMI       PshAI       PstI        
PvuII       RcaI        RsaI        SapI        Sau3AI      
Sau96I      ScrFI       SexAI       SfaNI       SfcI        
SmlI        SphI        StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XcmI        XhoII       
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