Restriction map for pF1KSDB1439
 (Linear) (Six-base) MAPSORT of: KIBB1439.seq  Check: 8335  from: 1  to: 6819



KIBB1439.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:29  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1221    4454    6819 
   Size:       1221    3233    2365

  Fragments arranged by size: 

                3233    2365    1221
 
AatII G_ACGT'C

Cuts at:      0    4298    6819 
   Size:       4298    2521
 
AccI GT'mk_AC

Cuts at:      0     533    6185    6804    6819 
   Size:        533    5652     619      15

  Fragments arranged by size: 

                5652     619     533      15
 
Acc65I G'GTAC_C

Cuts at:      0    2564    6819 
   Size:       2564    4255
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     619     630    1252    1445    2065    2389    3334    3460 
   Size:        619      11     622     193     620     324     945     126

Cuts at:   3460    3841    4233    4354    4853    5012    5786    6044    6819 
   Size:        381     392     121     499     159     774     258     775

  Fragments arranged by size: 

                 945     775     774     622     620     619     499     392
                 381     324     258     193     159     126     121      11
            
 
AfeI AGC'GCT

Cuts at:      0     634    6819 
   Size:        634    6185
 
AflIII A'CryG_T

Cuts at:      0    1266    1634    1664    3559    6819 
   Size:       1266     368      30    1895    3260

  Fragments arranged by size: 

                3260    1895    1266     368      30
 
AhdI GACnn_n'nnGTC

Cuts at:      0    6761    6819 
   Size:       6761      58
 
AleI CACnn'nnGTG

Cuts at:      0    5029    5968    6819 
   Size:       5029     939     851

  Fragments arranged by size: 

                5029     939     851
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1621    1653    2589    2621    3674    3706    6819 
   Size:       1621      32     936      32    1053      32    3113

  Fragments arranged by size: 

                3113    1621    1053     936      32      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1128    4071    6819 
   Size:       1128    2943    2748

  Fragments arranged by size: 

                2943    2748    1128
 
ApaI G_GGCC'C

Cuts at:      0    1307    1456    4777    6819 
   Size:       1307     149    3321    2042

  Fragments arranged by size: 

                3321    2042    1307     149
 
ApoI r'AATT_y

Cuts at:      0     848    2497    3788    4559    4645    4676    6478    6738 
   Size:        848    1649    1291     771      86      31    1802     260

Cuts at:   6738    6819 
   Size:         81

  Fragments arranged by size: 

                1802    1649    1291     848     771     260      86      81
                  31
 
AvaI C'yCGr_G

Cuts at:      0     979     995    1457    3285    4507    4945    6785    6819 
   Size:        979      16     462    1828    1222     438    1840      34

  Fragments arranged by size: 

                1840    1828    1222     979     462     438      34      16
            
 
BamHI G'GATC_C

Cuts at:      0    1068    2715    6819 
   Size:       1068    1647    4104

  Fragments arranged by size: 

                4104    1647    1068
 
BanI G'GyrC_C

Cuts at:      0     703    2564    3424    3969    4513    5845    5923    6436 
   Size:        703    1861     860     545     544    1332      78     513

Cuts at:   6436    6454    6819 
   Size:         18     365

  Fragments arranged by size: 

                1861    1332     860     703     545     544     513     365
                  78      18
 
BanII G_rGCy'C

Cuts at:      0    1307    1456    2679    2696    2819    4777    4945    5158 
   Size:       1307     149    1223      17     123    1958     168     213

Cuts at:   5158    5726    5738    6290    6819 
   Size:        568      12     552     529

  Fragments arranged by size: 

                1958    1307    1223     568     552     529     213     168
                 149     123      17      12
 
BbeI G_GCGC'C

Cuts at:      0    3973    6440    6819 
   Size:       3973    2467     379

  Fragments arranged by size: 

                3973    2467     379
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     728    1028    4390    4841    5496    6819 
   Size:        728     300    3362     451     655    1323

  Fragments arranged by size: 

                3362    1323     728     655     451     300
 
BbvCI CC'TCA_GC

Cuts at:      0     186    5485    6819 
   Size:        186    5299    1334

  Fragments arranged by size: 

                5299    1334     186
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      49      83    3887    3921    5220    5254    6130    6164 
   Size:         49      34    3804      34    1299      34     876      34

Cuts at:   6164    6819 
   Size:        655

  Fragments arranged by size: 

                3804    1299     876     655      49      34      34      34
                  34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1220    1679    4622    6819 
   Size:       1220     459    2943    2197

  Fragments arranged by size: 

                2943    2197    1220     459
 
BclI T'GATC_A

Cuts at:      0     542    1349    1943    2571    6819 
   Size:        542     807     594     628    4248

  Fragments arranged by size: 

                4248     807     628     594     542
 
BfrBI ATG'CAT

Cuts at:      0    1114    2160    6819 
   Size:       1114    1046    4659

  Fragments arranged by size: 

                4659    1114    1046
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2278    5290    5377    6203    6819 
   Size:       2278    3012      87     826     616

  Fragments arranged by size: 

                3012    2278     826     616      87
 
BglII A'GATC_T

Cuts at:      0     811    1310    6819 
   Size:        811     499    5509

  Fragments arranged by size: 

                5509     811     499
 
BlpI GC'TnA_GC

Cuts at:      0    2251    2363    3062    3869    6819 
   Size:       2251     112     699     807    2950

  Fragments arranged by size: 

                2950    2251     807     699     112
 
Bme1580I G_kGCm'C

Cuts at:      0     706    1307    1456    1614    4018    4507    4777    5848 
   Size:        706     601     149     158    2404     489     270    1071

Cuts at:   5848    6457    6819 
   Size:        609     362

  Fragments arranged by size: 

                2404    1071     706     609     601     489     362     270
                 158     149
 
BmgBI CAC'GTC

Cuts at:      0    1728    3573    6819 
   Size:       1728    1845    3246

  Fragments arranged by size: 

                3246    1845    1728
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    4693    5178    5622    5966    6819 
   Size:       4693     485     444     344     853

  Fragments arranged by size: 

                4693     853     485     444     344
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    4711    5019    5610    6717    6819 
   Size:       4711     308     591    1107     102

  Fragments arranged by size: 

                4711    1107     591     308     102
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     193     649    1150    1515    1570    1645    1868    1885 
   Size:        193     456     501     365      55      75     223      17

Cuts at:   1885    3139    3572    3769    4039    5119    5252    5835    5912 
   Size:       1254     433     197     270    1080     133     583      77

Cuts at:   5912    6177    6601    6815    6819 
   Size:        265     424     214       4

  Fragments arranged by size: 

                1254    1080     583     501     456     433     424     365
                 270     265     223     214     197     193     133      77
                  75      55      17       4
 
Bpu10I CC'TnA_GC

Cuts at:      0     186    3176    3324    3420    5467    5485    6819 
   Size:        186    2990     148      96    2047      18    1334

  Fragments arranged by size: 

                2990    2047    1334     186     148      96      18
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     536     779    5832    6454    6819 
   Size:        536     243    5053     622     365

  Fragments arranged by size: 

                5053     622     536     365     243
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     922    2044    3778    6633    6819 
   Size:        922    1122    1734    2855     186

  Fragments arranged by size: 

                2855    1734    1122     922     186
 
BsaAI yAC'GTr

Cuts at:      0    1698    3560    6209    6819 
   Size:       1698    1862    2649     610

  Fragments arranged by size: 

                2649    1862    1698     610
 
BsaBI GATnn'nnATC

Cuts at:      0     949    2131    2152    6819 
   Size:        949    1182      21    4667

  Fragments arranged by size: 

                4667    1182     949      21
 
BsaHI Gr'CG_yC

Cuts at:      0    3970    4295    6437    6819 
   Size:       3970     325    2142     382

  Fragments arranged by size: 

                3970    2142     382     325
 
BsaWI w'CCGG_w

Cuts at:      0     416    1802    2394    4350    5456    6819 
   Size:        416    1386     592    1956    1106    1363

  Fragments arranged by size: 

                1956    1386    1363    1106     592     416
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1984    2014    2020    2050    2098    2128    6819 
   Size:       1984      30       6      30      48      30    4691

  Fragments arranged by size: 

                4691    1984      48      30      30      30       6
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     325    3121    3766    4963    5602    6621    6624    6627 
   Size:        325    2796     645    1197     639    1019       3       3

Cuts at:   6627    6652    6655    6709    6819 
   Size:         25       3      54     110

  Fragments arranged by size: 

                2796    1197    1019     645     639     325     110      54
                  25       3       3       3
 
BseYI C'CCAG_C

Cuts at:      0    2899    3083    3113    4412    5079    5215    5337    5362 
   Size:       2899     184      30    1299     667     136     122      25

Cuts at:   5362    5856    6819 
   Size:        494     963

  Fragments arranged by size: 

                2899    1299     963     667     494     184     136     122
                  30      25
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1891    4084    4488    6610    6763    6819 
   Size:       1891    2193     404    2122     153      56

  Fragments arranged by size: 

                2193    2122    1891     404     153      56
 
BsiEI CG_ry'CG

Cuts at:      0     871    6819 
   Size:        871    5948
 
BsiHKAI G_wGCw'C

Cuts at:      0     691    1004    2726    2963    3068    4136    5594    6819 
   Size:        691     313    1722     237     105    1068    1458    1225

  Fragments arranged by size: 

                1722    1458    1225    1068     691     313     237     105
            
 
BsmI GAATG_Cn'

Cuts at:      0     135     163     200     348    1242    2878    6819 
   Size:        135      28      37     148     894    1636    3941

  Fragments arranged by size: 

                3941    1636     894     148     135      37      28
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     648    3282    3748    6819 
   Size:        648    2634     466    3071

  Fragments arranged by size: 

                3071    2634     648     466
 
Bsp1286I G_dGCh'C

Cuts at:      0     691     706    1004    1307    1456    1614    2679    2696 
   Size:        691      15     298     303     149     158    1065      17

Cuts at:   2696    2726    2819    2963    3068    4018    4136    4507    4777 
   Size:         30      93     144     105     950     118     371     270

Cuts at:   4777    4945    5158    5594    5726    5738    5848    6290    6457 
   Size:        168     213     436     132      12     110     442     167

Cuts at:   6457    6819 
   Size:        362

  Fragments arranged by size: 

                1065     950     691     442     436     371     362     303
                 298     270     213     168     167     158     149     144
                 132     118     110     105      93      30      17      15
                  12
 
BspEI T'CCGG_A

Cuts at:      0     416    4350    6819 
   Size:        416    3934    2469

  Fragments arranged by size: 

                3934    2469     416
 
BspHI T'CATG_A

Cuts at:      0     153     338    1940    4757    5195    6765    6819 
   Size:        153     185    1602    2817     438    1570      54

  Fragments arranged by size: 

                2817    1602    1570     438     185     153      54
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1221    2327    3535    4454    6539    6819 
   Size:       1221    1106    1208     919    2085     280

  Fragments arranged by size: 

                2085    1221    1208    1106     919     280
 
BsrBI CCG'CTC

Cuts at:      0     141    4769    5112    6819 
   Size:        141    4628     343    1707

  Fragments arranged by size: 

                4628    1707     343     141
 
BsrDI GCAATG_nn'

Cuts at:      0     231    3218    3386    3395    6819 
   Size:        231    2987     168       9    3424

  Fragments arranged by size: 

                3424    2987     231     168       9
 
BsrFI r'CCGG_y

Cuts at:      0    3972    5926    6819 
   Size:       3972    1954     893

  Fragments arranged by size: 

                3972    1954     893
 
BsrGI T'GTAC_A

Cuts at:      0    1196    6819 
   Size:       1196    5623
 
BssSI C'ACGA_G

Cuts at:      0      85    6819 
   Size:         85    6734
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     885    2526    4071    4131    4328    6819 
   Size:        885    1641    1545      60     197    2491

  Fragments arranged by size: 

                2491    1641    1545     885     197      60
 
BstEII G'GTnAC_C

Cuts at:      0     894    5172    6819 
   Size:        894    4278    1647

  Fragments arranged by size: 

                4278    1647     894
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     364    2609    5031    5649    6300    6819 
   Size:        364    2245    2422     618     651     519

  Fragments arranged by size: 

                2422    2245     651     618     519     364
 
BstYI r'GATC_y

Cuts at:      0     215     811    1068    1310    1709    1988    2715    3303 
   Size:        215     596     257     242     399     279     727     588

Cuts at:   3303    6650    6819 
   Size:       3347     169

  Fragments arranged by size: 

                3347     727     596     588     399     279     257     242
                 215     169
 
Bsu36I CC'TnA_GG

Cuts at:      0    4931    5976    6819 
   Size:       4931    1045     843

  Fragments arranged by size: 

                4931    1045     843
 
BtgI C'CryG_G

Cuts at:      0     202    1790    2483    5642    6819 
   Size:        202    1588     693    3159    1177

  Fragments arranged by size: 

                3159    1588    1177     693     202
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     193     591    6231    6819 
   Size:        193     398    5640     588

  Fragments arranged by size: 

                5640     588     398     193
 
BtsI GCAGTG_nn'

Cuts at:      0    2087    2222    2464    3522    4275    5293    5424    5867 
   Size:       2087     135     242    1058     753    1018     131     443

Cuts at:   5867    6591    6819 
   Size:        724     228

  Fragments arranged by size: 

                2087    1058    1018     753     724     443     242     228
                 135     131
 
ClaI AT'CG_AT

Cuts at:      0    6068    6819 
   Size:       6068     751
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    3644    3679    6819 
   Size:       3644      35    3140

  Fragments arranged by size: 

                3644    3140      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    3560    6819 
   Size:       3560    3259
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     529    3952    6819 
   Size:        529    3423    2867

  Fragments arranged by size: 

                3423    2867     529
 
EaeI y'GGCC_r

Cuts at:      0     366     868    1481    2823    4749    6419    6819 
   Size:        366     502     613    1342    1926    1670     400

  Fragments arranged by size: 

                1926    1670    1342     613     502     400     366
 
EagI C'GGCC_G

Cuts at:      0     868    6819 
   Size:        868    5951
 
EarI CTCTTCn'nnn_

Cuts at:      0     438     763    2008    2203    2432    3164    3439    3803 
   Size:        438     325    1245     195     229     732     275     364

Cuts at:   3803    6819 
   Size:       3016

  Fragments arranged by size: 

                3016    1245     732     438     364     325     275     229
                 195
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1519    1891    6819 
   Size:       1519     372    4928

  Fragments arranged by size: 

                4928    1519     372
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     193     619     630     649    1150    1252    1445    1515 
   Size:        193     426      11      19     501     102     193      70

Cuts at:   1515    1570    1645    1868    1885    2065    2389    3139    3334 
   Size:         55      75     223      17     180     324     750     195

Cuts at:   3334    3460    3572    3769    3841    4039    4233    4354    4853 
   Size:        126     112     197      72     198     194     121     499

Cuts at:   4853    5012    5119    5252    5786    5835    5912    6044    6177 
   Size:        159     107     133     534      49      77     132     133

Cuts at:   6177    6601    6815    6819 
   Size:        424     214       4

  Fragments arranged by size: 

                 750     534     501     499     426     424     324     223
                 214     198     197     195     194     193     193     180
                 159     133     133     132     126     121     112     107
                 102      77      75      72      70      55      49      19
                  17      11       4
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     697    1447    2572    2701    4930    4990    6819 
   Size:        697     750    1125     129    2229      60    1829

  Fragments arranged by size: 

                2229    1829    1125     750     697     129      60
 
EcoO109I rG'GnC_Cy

Cuts at:      0     621     902    1303    1304    1452    1453    3180    3524 
   Size:        621     281     401       1     148       1    1727     344

Cuts at:   3524    3847    3913    4521    4773    4774    5529    5616    6819 
   Size:        323      66     608     252       1     755      87    1203

  Fragments arranged by size: 

                1727    1203     755     621     608     401     344     323
                 281     252     148      87      66       1       1       1
            
 
EcoRI G'AATT_C

Cuts at:      0     848    2497    6819 
   Size:        848    1649    4322

  Fragments arranged by size: 

                4322    1649     848
 
EcoRV GAT'ATC

Cuts at:      0     465    6819 
   Size:        465    6354
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      87    2070    3465    4332    4363    6456    6819 
   Size:         87    1983    1395     867      31    2093     363

  Fragments arranged by size: 

                2093    1983    1395     867     363      87      31
 
FspI TGC'GCA

Cuts at:      0    3481    6819 
   Size:       3481    3338
 
HaeII r_GCGC'y

Cuts at:      0     598     636    3869    3973    4072    5694    6440    6819 
   Size:        598      38    3233     104      99    1622     746     379

  Fragments arranged by size: 

                3233    1622     746     598     379     104      99      38
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     534     566    1668    1700    2098    2130    3268    3281 
   Size:        534      32    1102      32     398      32    1138      13

Cuts at:   3281    3300    3313    3872    3904    5303    5335    6747    6779 
   Size:         19      13     559      32    1399      32    1412      32

Cuts at:   6779    6819 
   Size:         40

  Fragments arranged by size: 

                1412    1399    1138    1102     559     534     398      40
                  32      32      32      32      32      32      19      13
                  13
 
HincII GTy'rAC

Cuts at:      0    3891    4446    6805    6819 
   Size:       3891     555    2359      14

  Fragments arranged by size: 

                3891    2359     555      14
 
KasI G'GCGC_C

Cuts at:      0    3969    6436    6819 
   Size:       3969    2467     383

  Fragments arranged by size: 

                3969    2467     383
 
KpnI G_GTAC'C

Cuts at:      0    2568    6819 
   Size:       2568    4251
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1869    3116    4113    4616    5859    6819 
   Size:       1869    1247     997     503    1243     960

  Fragments arranged by size: 

                1869    1247    1243     997     960     503
 
MscI TGG'CCA

Cuts at:      0    1483    2825    6421    6819 
   Size:       1483    1342    3596     398

  Fragments arranged by size: 

                3596    1483    1342     398
 
MslI CAynn'nnrTG

Cuts at:      0    1339    1726    2008    2125    2233    2521    3340    3575 
   Size:       1339     387     282     117     108     288     819     235

Cuts at:   3575    4762    4846    5029    5053    5326    5647    5968    6298 
   Size:       1187      84     183      24     273     321     321     330

Cuts at:   6298    6819 
   Size:        521

  Fragments arranged by size: 

                1339    1187     819     521     387     330     321     321
                 288     282     273     235     183     117     108      84
                  24
 
MspA1I CmG'CkG

Cuts at:      0    1958    2362    2833    3087    3117    3533    4047    4328 
   Size:       1958     404     471     254      30     416     514     281

Cuts at:   4328    4416    6270    6819 
   Size:         88    1854     549

  Fragments arranged by size: 

                1958    1854     549     514     471     416     404     281
                 254      88      30
 
NaeI GCC'GGC

Cuts at:      0    3974    5928    6819 
   Size:       3974    1954     891

  Fragments arranged by size: 

                3974    1954     891
 
NarI GG'CG_CC

Cuts at:      0    3970    6437    6819 
   Size:       3970    2467     382

  Fragments arranged by size: 

                3970    2467     382
 
NcoI C'CATG_G

Cuts at:      0    5642    6819 
   Size:       5642    1177
 
NdeI CA'TA_TG

Cuts at:      0    3726    6819 
   Size:       3726    3093
 
NgoMIV G'CCGG_C

Cuts at:      0    3972    5926    6819 
   Size:       3972    1954     893

  Fragments arranged by size: 

                3972    1954     893
 
NsiI A_TGCA'T

Cuts at:      0    1116    2162    6819 
   Size:       1116    1046    4657

  Fragments arranged by size: 

                4657    1116    1046
 
NspI r_CATG'y

Cuts at:      0    1638    1668    3021    3072    3198    4063    4876    6324 
   Size:       1638      30    1353      51     126     865     813    1448

Cuts at:   6324    6615    6819 
   Size:        291     204

  Fragments arranged by size: 

                1638    1448    1353     865     813     291     204     126
                  51      30
 
PasI CC'CwG_GG

Cuts at:      0     699    1362    3402    3964    6819 
   Size:        699     663    2040     562    2855

  Fragments arranged by size: 

                2855    2040     699     663     562
 
PciI A'CATG_T

Cuts at:      0    1634    1664    6819 
   Size:       1634      30    5155

  Fragments arranged by size: 

                5155    1634      30
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     405    1128    2950    4318    5168    6387    6819 
   Size:        405     723    1822    1368     850    1219     432

  Fragments arranged by size: 

                1822    1368    1219     850     723     432     405
 
PfoI T'CCnGG_A

Cuts at:      0     270     584     626    1562    2102    2583    3599    5105 
   Size:        270     314      42     936     540     481    1016    1506

Cuts at:   5105    6819 
   Size:       1714

  Fragments arranged by size: 

                1714    1506    1016     936     540     481     314     270
                  42
 
PmlI CAC'GTG

Cuts at:      0    1698    3560    6819 
   Size:       1698    1862    3259

  Fragments arranged by size: 

                3259    1862    1698
 
PpuMI rG'GwC_Cy

Cuts at:      0    3180    3524    3847    3913    5616    6819 
   Size:       3180     344     323      66    1703    1203

  Fragments arranged by size: 

                3180    1703    1203     344     323      66
 
PshAI GACnn'nnGTC

Cuts at:      0    3770    4441    5566    6819 
   Size:       3770     671    1125    1253

  Fragments arranged by size: 

                3770    1253    1125     671
 
PsiI TTA'TAA

Cuts at:      0    4624    6819 
   Size:       4624    2195
 
PspOMI G'GGCC_C

Cuts at:      0    1303    1452    4773    6819 
   Size:       1303     149    3321    2046

  Fragments arranged by size: 

                3321    2046    1303     149
 
PspXI vC'TCGA_Gb

Cuts at:      0    6785    6819 
   Size:       6785      34
 
PstI C_TGCA'G

Cuts at:      0     616    1218    1835    2459    3532    4465    6819 
   Size:        616     602     617     624    1073     933    2354

  Fragments arranged by size: 

                2354    1073     933     624     617     616     602
 
PvuII CAG'CTG

Cuts at:      0    2833    3087    3117    3533    4047    4416    6819 
   Size:       2833     254      30     416     514     369    2403

  Fragments arranged by size: 

                2833    2403     514     416     369     254      30
 
SalI G'TCGA_C

Cuts at:      0    6803    6819 
   Size:       6803      16
 
SanDI GG'GwC_CC

Cuts at:      0    3913    6819 
   Size:       3913    2906
 
SapI GCTCTTCn'nnn_

Cuts at:      0     763    2008    2432    6819 
   Size:        763    1245     424    4387

  Fragments arranged by size: 

                4387    1245     763     424
 
SbfI CC_TGCA'GG

Cuts at:      0    4465    6819 
   Size:       4465    2354
 
ScaI AGT'ACT

Cuts at:      0    6700    6819 
   Size:       6700     119
 
SexAI A'CCwGG_T

Cuts at:      0    2095    2423    6725    6819 
   Size:       2095     328    4302      94

  Fragments arranged by size: 

                4302    2095     328      94
 
SfcI C'TryA_G

Cuts at:      0     144     612     709    1214    1831    2289    2455    3528 
   Size:        144     468      97     505     617     458     166    1073

Cuts at:   3528    4461    6819 
   Size:        933    2358

  Fragments arranged by size: 

                2358    1073     933     617     505     468     458     166
                 144      97
 
SfoI GGC'GCC

Cuts at:      0    3971    6438    6819 
   Size:       3971    2467     381

  Fragments arranged by size: 

                3971    2467     381
 
SmlI C'TyrA_G

Cuts at:      0     551     794    3285    5811    6469    6785    6819 
   Size:        551     243    2491    2526     658     316      34

  Fragments arranged by size: 

                2526    2491     658     551     316     243      34
 
SphI G_CATG'C

Cuts at:      0    3198    4876    6324    6819 
   Size:       3198    1678    1448     495

  Fragments arranged by size: 

                3198    1678    1448     495
 
SspI AAT'ATT

Cuts at:      0     827    6018    6819 
   Size:        827    5191     801

  Fragments arranged by size: 

                5191     827     801
 
StuI AGG'CCT

Cuts at:      0    1039    2447    3149    4792    6819 
   Size:       1039    1408     702    1643    2027

  Fragments arranged by size: 

                2027    1643    1408    1039     702
 
StyI C'CwwG_G

Cuts at:      0     363    2267    2608    3012    3239    3375    3473    3768 
   Size:        363    1904     341     404     227     136      98     295

Cuts at:   3768    4746    4991    5642    6819 
   Size:        978     245     651    1177

  Fragments arranged by size: 

                1904    1177     978     651     404     363     341     295
                 245     227     136      98
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     882    1618    2704    2741    3998    4426    5160    6819 
   Size:        882     736    1086      37    1257     428     734    1659

  Fragments arranged by size: 

                1659    1257    1086     882     736     734     428      37
            
 
TatI w'GTAC_w

Cuts at:      0    1196    3464    4238    5663    6698    6819 
   Size:       1196    2268     774    1425    1035     121

  Fragments arranged by size: 

                2268    1425    1196    1035     774     121
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      73     105     379     411     874     906    3501    3533 
   Size:         73      32     274      32     463      32    2595      32

Cuts at:   3533    3546    3578    4034    4066    5857    5889    6819 
   Size:         13      32     456      32    1791      32     930

  Fragments arranged by size: 

                2595    1791     930     463     456     274      73      32
                  32      32      32      32      32      32      13
 
XbaI T'CTAG_A

Cuts at:      0    4824    6819 
   Size:       4824    1995
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2274    2896    3225    3270    3354    5143    5859    6297 
   Size:       2274     622     329      45      84    1789     716     438

Cuts at:   6297    6819 
   Size:        522

  Fragments arranged by size: 

                2274    1789     716     622     522     438     329      84
                  45
 
XhoI C'TCGA_G

Cuts at:      0    3285    6785    6819 
   Size:       3285    3500      34

  Fragments arranged by size: 

                3500    3285      34
 
XmnI GAAnn'nnTTC

Cuts at:      0    5276    6819 
   Size:       5276    1543
 
ZraI GAC'GTC

Cuts at:      0    4296    6819 
   Size:       4296    2523
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AcuI     AfeI   AflIII     AhdI
     AleI     AloI    AlwNI     ApaI     ApoI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI
   BstAPI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FspI    HaeII
    Hin4I   HincII     KasI     KpnI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NsiI     NspI     PasI
     PciI    PflMI     PfoI     PmlI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PstI    PvuII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfoI     SmlI     SphI     SspI     StuI     StyI
    TaqII     TatI     TstI     XbaI     XcmI     XhoI     XmnI     ZraI

 Enzymes that do not cut: 

     AclI    AflII     AgeI     AlfI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI     BmtI    BsiWI   BssHII    BstBI  BstZ17I     DraI
  EcoICRI     FseI    FspAI  HindIII     HpaI     MfeI     MluI     NheI
     NotI     NruI     PacI     PmeI     PpiI     PsrI     PvuI    RsrII
     SacI    SacII     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SrfI     SwaI  Tth111I     XmaI
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