Restriction map for pF1KSDB0050
 (Linear) (Six-base) MAPSORT of: KIBB0050.seq  Check: 4926  from: 1  to: 3870



KIBB0050.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:29  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1911    3870 
   Size:       1911    1959
 
AccI GT'mk_AC

Cuts at:      0    2873    3870 
   Size:       2873     997
 
Acc65I G'GTAC_C

Cuts at:      0    2003    3870 
   Size:       2003    1867
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     550    2834    2986    3568    3651    3870 
   Size:        550    2284     152     582      83     219

  Fragments arranged by size: 

                2284     582     550     219     152      83
 
AflII C'TTAA_G

Cuts at:      0    1363    3870 
   Size:       1363    2507
 
AflIII A'CryG_T

Cuts at:      0     392    1007    2936    3494    3870 
   Size:        392     615    1929     558     376

  Fragments arranged by size: 

                1929     615     558     392     376
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     959    1645    3870 
   Size:        959     686    2225

  Fragments arranged by size: 

                2225     959     686
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     526     558    3870 
   Size:        526      32    3312

  Fragments arranged by size: 

                3312     526      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1966    3051    3176    3870 
   Size:       1966    1085     125     694

  Fragments arranged by size: 

                1966    1085     694     125
 
ApaI G_GGCC'C

Cuts at:      0      41    2319    3291    3834    3870 
   Size:         41    2278     972     543      36

  Fragments arranged by size: 

                2278     972     543      41      36
 
ApoI r'AATT_y

Cuts at:      0     340    3457    3510    3870 
   Size:        340    3117      53     360

  Fragments arranged by size: 

                3117     360     340      53
 
AscI GG'CGCG_CC

Cuts at:      0      44    3870 
   Size:         44    3826
 
AvaI C'yCGr_G

Cuts at:      0      85     136    3731    3845    3870 
   Size:         85      51    3595     114      25

  Fragments arranged by size: 

                3595     114      85      51      25
 
AvrII C'CTAG_G

Cuts at:      0    2601    3870 
   Size:       2601    1269
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1809    1842    3870 
   Size:       1809      33    2028

  Fragments arranged by size: 

                2028    1809      33
 
BanI G'GyrC_C

Cuts at:      0       7      94     193     438    1422    1923    2003    3870 
   Size:          7      87      99     245     984     501      80    1867

  Fragments arranged by size: 

                1867     984     501     245      99      87      80       7
            
 
BanII G_rGCy'C

Cuts at:      0      41    1346    1604    1734    2319    2858    3291    3697 
   Size:         41    1305     258     130     585     539     433     406

Cuts at:   3697    3732    3834    3870 
   Size:         35     102      36

  Fragments arranged by size: 

                1305     585     539     433     406     258     130     102
                  41      36      35
 
BbeI G_GCGC'C

Cuts at:      0      11     197    3870 
   Size:         11     186    3673

  Fragments arranged by size: 

                3673     186      11
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     151     259     682    2679    3385    3497    3556    3870 
   Size:        151     108     423    1997     706     112      59     314

  Fragments arranged by size: 

                1997     706     423     314     151     112     108      59
            
 
BbvCI CC'TCA_GC

Cuts at:      0    1400    2123    3870 
   Size:       1400     723    1747

  Fragments arranged by size: 

                1747    1400     723
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     420    1526    1837    3550    3870 
   Size:        420    1106     311    1713     320

  Fragments arranged by size: 

                1713    1106     420     320     311
 
BclI T'GATC_A

Cuts at:      0    3617    3870 
   Size:       3617     253
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1207    3870 
   Size:       1207    2663
 
BglII A'GATC_T

Cuts at:      0    1328    1547    3870 
   Size:       1328     219    2323

  Fragments arranged by size: 

                2323    1328     219
 
BlpI GC'TnA_GC

Cuts at:      0     358     641     832    1694    2033    2300    3870 
   Size:        358     283     191     862     339     267    1570

  Fragments arranged by size: 

                1570     862     358     339     283     267     191
 
Bme1580I G_kGCm'C

Cuts at:      0      41      97    1145    1928    2319    3291    3834    3870 
   Size:         41      56    1048     783     391     972     543      36

  Fragments arranged by size: 

                1048     972     783     543     391      56      41      36
            
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1216    2772    3529    3870 
   Size:       1216    1556     757     341

  Fragments arranged by size: 

                1556    1216     757     341
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1650    3624    3711    3870 
   Size:       1650    1974      87     159

  Fragments arranged by size: 

                1974    1650     159      87
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     184     622     796    1017    1510    2302    2584    2872 
   Size:        184     438     174     221     493     792     282     288

Cuts at:   2872    3274    3371    3619    3870 
   Size:        402      97     248     251

  Fragments arranged by size: 

                 792     493     438     402     288     282     251     248
                 221     184     174      97
 
Bpu10I CC'TnA_GC

Cuts at:      0    1400    2123    3521    3870 
   Size:       1400     723    1398     349

  Fragments arranged by size: 

                1400    1398     723     349
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     839    1558    1717    1759    3870 
   Size:        839     719     159      42    2111

  Fragments arranged by size: 

                2111     839     719     159      42
 
BsaAI yAC'GTr

Cuts at:      0     182    1010    2535    2973    3186    3870 
   Size:        182     828    1525     438     213     684

  Fragments arranged by size: 

                1525     828     684     438     213     182
 
BsaBI GATnn'nnATC

Cuts at:      0     275     497    1378    2650    3870 
   Size:        275     222     881    1272    1220

  Fragments arranged by size: 

                1272    1220     881     275     222
 
BsaHI Gr'CG_yC

Cuts at:      0       8      56     194    1683    2704    2801    3870 
   Size:          8      48     138    1489    1021      97    1069

  Fragments arranged by size: 

                1489    1069    1021     138      97      48       8
 
BsaWI w'CCGG_w

Cuts at:      0     470    1726    3870 
   Size:        470    1256    2144

  Fragments arranged by size: 

                2144    1256     470
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     976    1006    1015    1045    3870 
   Size:        976      30       9      30    2825

  Fragments arranged by size: 

                2825     976      30      30       9
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     880     984     987    1300    1432    1576    1609    1612 
   Size:        880     104       3     313     132     144      33       3

Cuts at:   1612    2287    2764    3870 
   Size:        675     477    1106

  Fragments arranged by size: 

                1106     880     675     477     313     144     132     104
                  33       3       3
 
BseYI C'CCAG_C

Cuts at:      0     288     718    1103    1259    1296    1846    3282    3870 
   Size:        288     430     385     156      37     550    1436     588

  Fragments arranged by size: 

                1436     588     550     430     385     288     156      37
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     838    1165    1615    2402    3870 
   Size:         52     786     327     450     787    1468

  Fragments arranged by size: 

                1468     787     786     450     327      52
 
BsiEI CG_ry'CG

Cuts at:      0     301     730    3870 
   Size:        301     429    3140

  Fragments arranged by size: 

                3140     429     301
 
BsiHKAI G_wGCw'C

Cuts at:      0    2028    2039    2582    2858    3870 
   Size:       2028      11     543     276    1012

  Fragments arranged by size: 

                2028    1012     543     276      11
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     160    3870 
   Size:        160    3710
 
Bsp1286I G_dGCh'C

Cuts at:      0      41      97    1145    1346    1604    1734    1928    2028 
   Size:         41      56    1048     201     258     130     194     100

Cuts at:   2028    2039    2319    2582    2858    3291    3697    3732    3834 
   Size:         11     280     263     276     433     406      35     102

Cuts at:   3834    3870 
   Size:         36

  Fragments arranged by size: 

                1048     433     406     280     276     263     258     201
                 194     130     102     100      56      41      36      35
                  11
 
BspEI T'CCGG_A

Cuts at:      0     470    3870 
   Size:        470    3400
 
BspHI T'CATG_A

Cuts at:      0     101    3161    3870 
   Size:        101    3060     709

  Fragments arranged by size: 

                3060     709     101
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1911    3870 
   Size:       1911    1959
 
BsrBI CCG'CTC

Cuts at:      0     141     588     726    3690    3870 
   Size:        141     447     138    2964     180

  Fragments arranged by size: 

                2964     447     180     141     138
 
BsrDI GCAATG_nn'

Cuts at:      0     312     430    1253    2139    3870 
   Size:        312     118     823     886    1731

  Fragments arranged by size: 

                1731     886     823     312     118
 
BsrFI r'CCGG_y

Cuts at:      0    2634    2675    2879    3870 
   Size:       2634      41     204     991

  Fragments arranged by size: 

                2634     991     204      41
 
BsrGI T'GTAC_A

Cuts at:      0     662    3870 
   Size:        662    3208
 
BssHII G'CGCG_C

Cuts at:      0      44    3870 
   Size:         44    3826
 
BssSI C'ACGA_G

Cuts at:      0    1750    1987    2057    3870 
   Size:       1750     237      70    1813

  Fragments arranged by size: 

                1813    1750     237      70
 
BstEII G'GTnAC_C

Cuts at:      0    3652    3870 
   Size:       3652     218
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1323    2700    3870 
   Size:       1323    1377    1170

  Fragments arranged by size: 

                1377    1323    1170
 
BstYI r'GATC_y

Cuts at:      0     239     989    1328    1373    1547    2774    3086    3870 
   Size:        239     750     339      45     174    1227     312     784

  Fragments arranged by size: 

                1227     784     750     339     312     239     174      45
            
 
Bsu36I CC'TnA_GG

Cuts at:      0    2660    3705    3870 
   Size:       2660    1045     165

  Fragments arranged by size: 

                2660    1045     165
 
BtgI C'CryG_G

Cuts at:      0    1137    1322    1653    2555    3246    3870 
   Size:       1137     185     331     902     691     624

  Fragments arranged by size: 

                1137     902     691     624     331     185
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1674    3870 
   Size:       1674    2196
 
BtsI GCAGTG_nn'

Cuts at:      0    1245    1413    1578    1818    3572    3870 
   Size:       1245     168     165     240    1754     298

  Fragments arranged by size: 

                1754    1245     298     240     168     165
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    2516    2551    3870 
   Size:       2516      35    1319

  Fragments arranged by size: 

                2516    1319      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1879    3870 
   Size:       1879    1991
 
EaeI y'GGCC_r

Cuts at:      0     298     727    3309    3418    3723    3870 
   Size:        298     429    2582     109     305     147

  Fragments arranged by size: 

                2582     429     305     298     147     109
 
EagI C'GGCC_G

Cuts at:      0     298     727    3870 
   Size:        298     429    3143

  Fragments arranged by size: 

                3143     429     298
 
EarI CTCTTCn'nnn_

Cuts at:      0    2534    2686    2894    3163    3870 
   Size:       2534     152     208     269     707

  Fragments arranged by size: 

                2534     707     269     208     152
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     232    1815    3870 
   Size:        232    1583    2055

  Fragments arranged by size: 

                2055    1583     232
 
EcoICRI GAG'CTC

Cuts at:      0    2856    3870 
   Size:       2856    1014
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     184     550     622     796    1017    1510    2302    2584 
   Size:        184     366      72     174     221     493     792     282

Cuts at:   2584    2834    2872    2986    3274    3371    3568    3619    3651 
   Size:        250      38     114     288      97     197      51      32

Cuts at:   3651    3870 
   Size:        219

  Fragments arranged by size: 

                 792     493     366     288     282     250     221     219
                 197     184     174     114      97      72      51      38
                  32
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2323    3520    3870 
   Size:       2323    1197     350

  Fragments arranged by size: 

                2323    1197     350
 
EcoO109I rG'GnC_Cy

Cuts at:      0     285     525     561    1494    1850    2066    2315    2316 
   Size:        285     240      36     933     356     216     249       1

Cuts at:   2316    3153    3287    3594    3830    3831    3870 
   Size:        837     134     307     236       1      39

  Fragments arranged by size: 

                 933     837     356     307     285     249     240     236
                 216     134      39      36       1       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1881    3870 
   Size:       1881    1989
 
HaeII r_GCGC'y

Cuts at:      0      11     197     671    1701    2447    3870 
   Size:         11     186     474    1030     746    1423

  Fragments arranged by size: 

                1423    1030     746     474     186      11
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     499     531    1015    1047    1065    1097    2926    2958 
   Size:        499      32     484      32      18      32    1829      32

Cuts at:   2958    3005    3037    3870 
   Size:         47      32     833

  Fragments arranged by size: 

                1829     833     499     484      47      32      32      32
                  32      32      18
 
HincII GTy'rAC

Cuts at:      0     552    2205    2550    3870 
   Size:        552    1653     345    1320

  Fragments arranged by size: 

                1653    1320     552     345
 
KasI G'GCGC_C

Cuts at:      0       7     193    3870 
   Size:          7     186    3677

  Fragments arranged by size: 

                3677     186       7
 
KpnI G_GTAC'C

Cuts at:      0    2007    3870 
   Size:       2007    1863
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1076    3086    3870 
   Size:       1076    2010     784

  Fragments arranged by size: 

                2010    1076     784
 
MscI TGG'CCA

Cuts at:      0    3420    3870 
   Size:       3420     450
 
MslI CAynn'nnrTG

Cuts at:      0     100    1637    2044    2144    2698    3870 
   Size:        100    1537     407     100     554    1172

  Fragments arranged by size: 

                1537    1172     554     407     100     100
 
MspA1I CmG'CkG

Cuts at:      0     614     645     939    2299    2514    3248    3714    3870 
   Size:        614      31     294    1360     215     734     466     156

  Fragments arranged by size: 

                1360     734     614     466     294     215     156      31
            
 
NaeI GCC'GGC

Cuts at:      0    2636    2881    3870 
   Size:       2636     245     989

  Fragments arranged by size: 

                2636     989     245
 
NarI GG'CG_CC

Cuts at:      0       8     194    3870 
   Size:          8     186    3676

  Fragments arranged by size: 

                3676     186       8
 
NcoI C'CATG_G

Cuts at:      0    1137    1653    3870 
   Size:       1137     516    2217

  Fragments arranged by size: 

                2217    1137     516
 
NdeI CA'TA_TG

Cuts at:      0    2103    3870 
   Size:       2103    1767
 
NgoMIV G'CCGG_C

Cuts at:      0    2634    2879    3870 
   Size:       2634     245     991

  Fragments arranged by size: 

                2634     991     245
 
NspI r_CATG'y

Cuts at:      0     396     540     573    1201    2043    2940    3498    3870 
   Size:        396     144      33     628     842     897     558     372

  Fragments arranged by size: 

                 897     842     628     558     396     372     144      33
            
 
PasI CC'CwG_GG

Cuts at:      0     247    3870 
   Size:        247    3623
 
PciI A'CATG_T

Cuts at:      0     392    2936    3494    3870 
   Size:        392    2544     558     376

  Fragments arranged by size: 

                2544     558     392     376
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2460    3051    3307    3870 
   Size:       2460     591     256     563

  Fragments arranged by size: 

                2460     591     563     256
 
PfoI T'CCnGG_A

Cuts at:      0     881    2948    3870 
   Size:        881    2067     922

  Fragments arranged by size: 

                2067     922     881
 
PmlI CAC'GTG

Cuts at:      0     182    3870 
   Size:        182    3688
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1633    1665    3870 
   Size:       1633      32    2205

  Fragments arranged by size: 

                2205    1633      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     285     525     561    1494    1850    3594    3870 
   Size:        285     240      36     933     356    1744     276

  Fragments arranged by size: 

                1744     933     356     285     276     240      36
 
PshAI GACnn'nnGTC

Cuts at:      0    1682    3659    3847    3870 
   Size:       1682    1977     188      23

  Fragments arranged by size: 

                1977    1682     188      23
 
PspOMI G'GGCC_C

Cuts at:      0      37    2315    3287    3830    3870 
   Size:         37    2278     972     543      40

  Fragments arranged by size: 

                2278     972     543      40      37
 
PspXI vC'TCGA_Gb

Cuts at:      0      85    3870 
   Size:         85    3785
 
PstI C_TGCA'G

Cuts at:      0     455    1252    2759    3020    3194    3870 
   Size:        455     797    1507     261     174     676

  Fragments arranged by size: 

                1507     797     676     455     261     174
 
PvuII CAG'CTG

Cuts at:      0     645     939    2299    2514    3714    3870 
   Size:        645     294    1360     215    1200     156

  Fragments arranged by size: 

                1360    1200     645     294     215     156
 
SacI G_AGCT'C

Cuts at:      0    2858    3870 
   Size:       2858    1012
 
SacII CC_GC'GG

Cuts at:      0    3249    3870 
   Size:       3249     621
 
SanDI GG'GwC_CC

Cuts at:      0     561    1850    3870 
   Size:        561    1289    2020

  Fragments arranged by size: 

                2020    1289     561
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2686    3870 
   Size:       2686    1184
 
ScaI AGT'ACT

Cuts at:      0     708    1714    3870 
   Size:        708    1006    2156

  Fragments arranged by size: 

                2156    1006     708
 
SfcI C'TryA_G

Cuts at:      0     451    1248    2755    3016    3190    3585    3870 
   Size:        451     797    1507     261     174     395     285

  Fragments arranged by size: 

                1507     797     451     395     285     261     174
 
SfoI GGC'GCC

Cuts at:      0       9     195    3870 
   Size:          9     186    3675

  Fragments arranged by size: 

                3675     186       9
 
SmaI CCC'GGG

Cuts at:      0    3733    3870 
   Size:       3733     137
 
SmlI C'TyrA_G

Cuts at:      0      85    1363    3870 
   Size:         85    1278    2507

  Fragments arranged by size: 

                2507    1278      85
 
SphI G_CATG'C

Cuts at:      0    1201    3870 
   Size:       1201    2669
 
StuI AGG'CCT

Cuts at:      0    3217    3545    3870 
   Size:       3217     328     325

  Fragments arranged by size: 

                3217     328     325
 
StyI C'CwwG_G

Cuts at:      0     521    1046    1137    1604    1653    2601    2957    3038 
   Size:        521     525      91     467      49     948     356      81

Cuts at:   3038    3641    3870 
   Size:        603     229

  Fragments arranged by size: 

                 948     603     525     521     467     356     229      91
                  81      49
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     456    1368    2754    3870 
   Size:        456     912    1386    1116

  Fragments arranged by size: 

                1386    1116     912     456
 
TatI w'GTAC_w

Cuts at:      0     662     706    1439    1712    3005    3870 
   Size:        662      44     733     273    1293     865

  Fragments arranged by size: 

                1293     865     733     662     273      44
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    3718    3870 
   Size:       3718     152
 
XhoI C'TCGA_G

Cuts at:      0      85    3870 
   Size:         85    3785
 
XmaI C'CCGG_G

Cuts at:      0    3731    3870 
   Size:       3731     139
 
XmnI GAAnn'nnTTC

Cuts at:      0     148    1648    3870 
   Size:        148    1500    2222

  Fragments arranged by size: 

                2222    1500     148
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI    AflII   AflIII     AlfI     AloI
    AlwNI     ApaI     ApoI     AscI     AvaI    AvrII     BaeI     BanI
    BanII     BbeI     BbsI    BbvCI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstEII    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI    CspCI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI    HaeII    Hin4I
   HincII     KasI     KpnI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NspI     PasI     PciI    PflMI
     PfoI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PstI
    PvuII     SacI    SacII    SanDI     SapI     ScaI     SfcI     SfoI
     SmaI     SmlI     SphI     StuI     StyI    TaqII     TatI     XcmI
     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AclI     AfeI     AgeI     AhdI     AleI    ApaLI     AseI
    AsiSI    BamHI     BcgI    BfrBI    BmgBI     BmtI    BpuEI    BsiWI
     BsmI   BstAPI    BstBI  BstZ17I     ClaI     DraI   DraIII    EcoRI
    EcoRV     FseI     FspI    FspAI  HindIII     HpaI     MfeI     MluI
     NheI     NotI     NruI     NsiI     PacI     PmeI     PsiI     PsrI
     PvuI    RsrII     SalI     SbfI    SexAI     SfiI     SgfI    SgrAI
    SnaBI     SpeI     SrfI     SspI     SwaI     TstI  Tth111I     XbaI
     ZraI
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