Restriction map for pF1KSDA2008
##################################
# NO CUT ENZYMES (   99 enzymes) #
##################################

AccIII      AclI        AcsI        AflII       AhdI        
Alw44I      AocI        Aor51HI     ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     AspEI       
AspI        BbsI        BbvCI       BcgI        BciVI       
BfrI        BglII       BlpI        Bpu1102I    BpuAI       
BsaBI       BseAI       BsiEI       BsiWI       BsmI        
Bsp1407I    BspDI       BspEI       BspLU11I    BsrDI       
BsrGI       BssHII      Bst1107I    BstBI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoRI       EcoRV       EcoT22I     HindIII     
HpaI        MamI        MfeI        MluI        MroI        
MseI        MunI        NdeI        NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PflFI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SbfI        ScaI        SexAI       
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tru9I       Tsp509I     Tth111I     XbaI        
XcmI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AatII         114  GACGTC               GACGT^C
Acc65I       1035  GGTACC               G^GTACC
AgeI         1154  ACCGGT               A^CCGGT
Asp718I      1035  GGTACC               G^GTACC
AvrII         930  CCTAGG               C^CTAGG
BbeI          143  GGCGCC               GGCGC^C
BbrPI        1777  CACGTG               CAC^GTG
BclI            2  TGATCA               T^GATCA
BfaI          931  CTAG                 C^TAG
BlnI          930  CCTAGG               C^CTAGG
BsaAI        1777  CACGTG               [CT]AC^GT[GA]
BsaI           57  GAGACC               GGTCTC(1/5)
BstEII         48  GGTCACC              G^GT[ACGT]ACC
BstPI          48  GGTCACC              G^GT[ACGT]ACC
BstXI        1376  CCAGCAACATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI          879  GACGGAGGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I        48  GGTCACC              G^GT[ACGT]ACC
FbaI            2  TGATCA               T^GATCA
FseI          342  GGCCGGCC             GGCCGG^CC
KasI          143  GGCGCC               G^GCGCC
KpnI         1035  GGTACC               GGTAC^C
KspI          201  CCGCGG               CCGC^GG
MaeI          931  CTAG                 C^TAG
NarI          143  GGCGCC               GG^CGCC
PaeR7I       2008  CTCGAG               C^TCGAG
PinAI        1154  ACCGGT               A^CCGGT
PmaCI        1777  CACGTG               CAC^GTG
PmlI         1777  CACGTG               CAC^GTG
PpuMI         911  GGGTCCT              [GA]G^G[AT]CC[CT]
SacII         201  CCGCGG               CCGC^GG
SalI         1789  GTCGAC               G^TCGAC
SapI         2280  GCTCTTC              GCTCTTC(1/4)
SfiI         2070  GGCCTGGCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI          143  GGCGCC               GGC^GCC
TfiI         1163  GAATC                G^A[AT]TC
XhoI         2008  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AccI         1789  GTCGAC               GT^[AC][GT]AC
             1849  GTAGAC              
AflIII        896  ACGTGT               A^C[GA][CT]GT
             1778  ACGTGT              
AviII         212  TGCGCA               TGC^GCA
             1571  TGCGCA              
BalI          263  TGGCCA               TGG^CCA
             2247  TGGCCA              
BamHI         359  GGATCC               G^GATCC
             1898  GGATCC              
BsaWI        1154  ACCGGT               [AT]^CCGG[AT]
             1235  ACCGGA              
BspHI         758  TCATGA               T^CATGA
             1277  TCATGA              
BssSI        1207  CACGAG               CACGAG(-5/-1)
             1696  CACGAG              
DraIII        476  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
              576  CACCGAGTG           
EarI         1585  CTCTTC               CTCTTC(1/4)
             2281  CTCTTC              
FspI          212  TGCGCA               TGC^GCA
             1571  TGCGCA              
HincII         10  GTCAAC               GT[CT]^[GA]AC
             1789  GTCGAC              
HindII         10  GTCAAC               GT[CT]^[GA]AC
             1789  GTCGAC              
Ksp632I      1585  CTCTTC               CTCTTC(1/4)
             2281  CTCTTC              
MluNI         263  TGGCCA               TGG^CCA
             2247  TGGCCA              
MscI          263  TGGCCA               TGG^CCA
             2247  TGGCCA              
NcoI          984  CCATGG               C^CATGG
             1473  CCATGG              
PflMI         714  CCACCTTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              807  CCAGCGCCTGG         
PvuII        1564  CAGCTG               CAG^CTG
             2286  CAGCTG              
RcaI          758  TCATGA               T^CATGA
             1277  TCATGA              
SacI         1333  GAGCTC               GAGCT^C
             1934  GAGCTC              
SmlI         2008  CTCGAG               C^T[CT][GA]AG
             2176  CTCAAG              
Van91I        714  CCACCTTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              807  CCAGCGCCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  100 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApaI        AspHI       AvaI        
AvaII       BanI        BanII       BbvI        BcnI        
BglI        BmyI        BpmI        BsaHI       BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrBI       BsrFI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstUI       BstYI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NaeI        
NciI        NdeII       NgoMIV      NlaIII      NlaIV       
PleI        PstI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StyI        TaqI        
TseI        Tsp45I      TspRI       TthHB8I     XhoII       
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