Restriction map for pF1KSDA1950
##################################
# NO CUT ENZYMES (  121 enzymes) #
##################################

AatII       AccIII      AclI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp700I     
AspEI       AspI        AvrII       BamHI       BbeI        
BbsI        BbvCI       BciVI       BclI        BfaI        
BfrI        BglII       BlnI        BpuAI       BsaBI       
BseAI       BsiWI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrDI       
BsrGI       BssHII      Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstXI       BstYI       BstZ17I     
ClaI        CpoI        DraI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoO65I     EcoRV       
EcoT14I     EcoT22I     FbaI        FseI        HincII      
HindII      HindIII     HpaI        KasI        KspI        
MaeI        MamI        MfeI        MflI        MluI        
MroI        MseI        MunI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmeI        PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SalI        
ScaI        SfiI        SfoI        SfuI        SgrAI       
SmlI        SnaBI       SpeI        SphI        SspBI       
SspI        StyI        SwaI        TfiI        Tru9I       
Tth111I     XbaI        XcmI        XhoI        XhoII       
XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

Acc65I        941  GGTACC               G^GTACC
AccI           11  GTAGAC               GT^[AC][GT]AC
AcsI          637  GAATTC               [GA]^AATT[CT]
ApoI          637  GAATTC               [GA]^AATT[CT]
Asp718I       941  GGTACC               G^GTACC
AspHI         784  GAGCTC               G[AT]GC[AT]^C
AviII        1388  TGCGCA               TGC^GCA
BalI         1232  TGGCCA               TGG^CCA
BbrPI         774  CACGTG               CAC^GTG
BcgI          182  GCACGGGCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI         1168  GCCTCAGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI         774  CACGTG               [CT]AC^GT[GA]
BsaI           63  GGTCTC               GGTCTC(1/5)
BsaWI         257  ACCGGA               [AT]^CCGG[AT]
BsiEI        1311  CGACCG               CG[GA][CT]^CG
BsiHKAI       784  GAGCTC               G[AT]GC[AT]^C
BspMI        1284  GCAGGT               ACCTGC(4/8)
BsrBI         118  GAGCGG               CCGCTC(-3/-3)
BsrFI         318  GCCGGT               [GA]^CCGG[CT]
BssSI         781  CACGAG               CACGAG(-5/-1)
Cfr10I        318  GCCGGT               [GA]^CCGG[CT]
DpnII         667  GATC                 ^GATC
DraIII        598  CACGAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI           55  GACGCTCTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI         1298  CCGTGG               C^C[GA][CT]GG
EcoRI         637  GAATTC               G^AATTC
FspI         1388  TGCGCA               TGC^GCA
KpnI          941  GGTACC               GGTAC^C
MboI          667  GATC                 ^GATC
MluNI        1232  TGGCCA               TGG^CCA
MscI         1232  TGGCCA               TGG^CCA
MslI         1083  CACCAGTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII         667  GATC                 ^GATC
PflMI         792  CCACCTTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI         774  CACGTG               CAC^GTG
PmlI          774  CACGTG               CAC^GTG
PshAI         880  GACCTCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI          784  GAGCTC               GAGCT^C
Sau3AI        667  GATC                 ^GATC
SbfI          711  CCTGCAGG             CCTGCA^GG
SexAI        1277  ACCTGGT              A^CC[AT]GGT
SmaI           41  CCCGGG               CCC^GGG
Sse8387I      711  CCTGCAGG             CCTGCA^GG
Tsp509I       638  AATT                 ^AATT
Van91I        792  CCACCTTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI          41  CCCGGG               C^CCGGG
XmaI           41  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AcyI          607  GGCGTC               G[GA]^CG[CT]C
             1303  GACGCC              
AfaI          549  GTAC                 GT^AC
              942  GTAC                
BanI          941  GGTACC               G^G[CT][GA]CC
             1081  GGCACC              
BlpI          846  GCTCAGC              GC^T[ACGT]AGC
             1152  GCTGAGC             
BpmI          457  CTCCAG               CTGGAG(16/14)
              564  CTGGAG              
Bpu1102I      846  GCTCAGC              GC^T[ACGT]AGC
             1152  GCTGAGC             
BsaHI         607  GGCGTC               G[GA]^CG[CT]C
             1303  GACGCC              
BsmAI         948  GAGAC                GTCTC(1/5)
               64  GTCTC               
BsrI          194  ACTGG                ACTGG(1/-1)
             1085  CCAGT               
CelII         846  GCTCAGC              GC^T[ACGT]AGC
             1152  GCTGAGC             
HaeII         658  AGCGCC               [GA]GCGC^[CT]
             1039  AGCGCC              
Hin1I         607  GGCGTC               G[GA]^CG[CT]C
             1303  GACGCC              
HphI          572  TCACC                GGTGA(8/7)
             1103  TCACC               
MaeII         775  ACGT                 A^CGT
              982  ACGT                
NspI          912  GCATGT               [GA]CATG^[CT]
             1052  GCATGT              
PpuMI        1274  AGGACCT              [GA]G^G[AT]CC[CT]
             1355  AGGACCC             
RsaI          549  GTAC                 GT^AC
              942  GTAC                
SapI          123  GCTCTTC              GCTCTTC(1/4)
              159  GCTCTTC             


#########################################
# 3 OR MORE CUT ENZYMES (   72 enzymes) #
#########################################

AccII       AciI        AluI        AlwNI       AocI        
ApaI        AvaI        AvaII       BanII       BbvI        
BcnI        BmyI        BsaJI       BseRI       BsgI        
BsiYI       BslI        BsmFI       BsoBI       Bsp1286I    
BssKI       BstF5I      BstNI       BstUI       Bsu36I      
Cac8I       CfoI        Cfr13I      DdeI        DraII       
EaeI        EarI        Eco81I      EcoNI       EcoO109I    
EcoRII      FauI        Fnu4HI      FokI        HaeIII      
HapII       HgaI        HhaI        HinP1I      HinfI       
HpaII       ItaI        Ksp632I     MaeIII      MboII       
MnlI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NlaIII      NlaIV       PleI        
PstI        PvuII       Sau96I      ScrFI       SfaNI       
SfcI        StuI        TaqI        TseI        Tsp45I      
TspRI       TthHB8I     
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