Restriction map for pF1KSDA1769
##################################
# NO CUT ENZYMES (   45 enzymes) #
##################################

AclI        AcsI        AflII       AhdI        ApoI        
AscI        AseI        AsnI        AspEI       BciVI       
BfrI        BsaBI       BsiWI       BsmI        BspHI       
BsrDI       BstBI       DraI        Eam1105I    EcoRI       
EcoRV       HindIII     HpaI        KspI        MamI        
MfeI        MluI        MunI        NheI        NotI        
NruI        NspV        PacI        PmeI        PshBI       
Psp1406I    RcaI        SacII       SalI        SfuI        
SnaBI       SpeI        SspI        SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   24 enzymes) #
###################################

AatII        2533  GACGTC               GACGT^C
Asp700I      4791  GAAGCCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII         705  TGCGCA               TGC^GCA
AvrII         843  CCTAGG               C^CTAGG
BclI         2684  TGATCA               T^GATCA
BglII        1967  AGATCT               A^GATCT
BlnI          843  CCTAGG               C^CTAGG
BspLU11I      668  ACATGT               A^CATGT
Bst1107I     4410  GTATAC               GTA^TAC
BstZ17I      4410  GTATAC               GTA^TAC
EcoT22I      5572  ATGCAT               ATGCA^T
FbaI         2684  TGATCA               T^GATCA
FspI          705  TGCGCA               TGC^GCA
MseI         2888  TTAA                 T^TAA
NdeI         5548  CATATG               CA^TATG
NsiI         5572  ATGCAT               ATGCA^T
PshAI        2374  GACCTGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI         4835  CGATCG               CGAT^CG
ScaI          149  AGTACT               AGT^ACT
SgrAI        4155  CACCGGCG             C[GA]^CCGG[CT]G
SphI         5570  GCATGC               GCATG^C
Tru9I        2888  TTAA                 T^TAA
Tsp509I      2097  AATT                 ^AATT
XmnI         4791  GAAGCCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AccI         3688  GTCTAC               GT^[AC][GT]AC
             4410  GTATAC              
AccIII       2018  TCCGGA               T^CCGGA
             4527  TCCGGA              
AgeI         4091  ACCGGT               A^CCGGT
             5513  ACCGGT              
Aor51HI      3221  AGCGCT               AGC^GCT
             3806  AGCGCT              
BamHI        3703  GGATCC               G^GATCC
             5150  GGATCC              
BfaI          844  CTAG                 C^TAG
             4621  CTAG                
BseAI        2018  TCCGGA               T^CCGGA
             4527  TCCGGA              
Bsp1407I      104  TGTACA               T^GTACA
             5024  TGTACA              
BspDI         289  ATCGAT               AT^CGAT
             3400  ATCGAT              
BspEI        2018  TCCGGA               T^CCGGA
             4527  TCCGGA              
BsrGI         104  TGTACA               T^GTACA
             5024  TGTACA              
BssHII       1659  GCGCGC               G^CGCGC
             3463  GCGCGC              
BstXI        1724  CCATCCCGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             5654  CCAGTGTCCTGG        
ClaI          289  ATCGAT               AT^CGAT
             3400  ATCGAT              
CpoI          110  CGGACCG              CG^G[AT]CCG
             2797  CGGTCCG             
DraIII        214  CACCGCGTG            CAC[ACGT][ACGT][ACGT]^GTG
              478  CACGAGGTG           
Eco47III     3221  AGCGCT               AGC^GCT
             3806  AGCGCT              
FseI         3652  GGCCGGCC             GGCCGG^CC
             4660  GGCCGGCC            
HincII       4396  GTTGAC               GT[CT]^[GA]AC
             5368  GTTGAC              
HindII       4396  GTTGAC               GT[CT]^[GA]AC
             5368  GTTGAC              
MaeI          844  CTAG                 C^TAG
             4621  CTAG                
MroI         2018  TCCGGA               T^CCGGA
             4527  TCCGGA              
PaeR7I       4273  CTCGAG               C^TCGAG
             4327  CTCGAG              
PinAI        4091  ACCGGT               A^CCGGT
             5513  ACCGGT              
RsrII         110  CGGACCG              CG^G[AT]CCG
             2797  CGGTCCG             
SbfI         3190  CCTGCAGG             CCTGCA^GG
             3939  CCTGCAGG            
SexAI        4818  ACCAGGT              A^CC[AT]GGT
             5237  ACCTGGT             
SfiI          417  GGCCCGGGCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             3652  GGCCGGCCGGGCC       
Sse8387I     3190  CCTGCAGG             CCTGCA^GG
             3939  CCTGCAGG            
SspBI         104  TGTACA               T^GTACA
             5024  TGTACA              
TfiI          690  GAATC                G^A[AT]TC
             2880  GATTC               
XhoI         4273  CTCGAG               C^TCGAG
             4327  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  157 enzymes) #
#########################################

Acc65I      AccII       AciI        AcyI        AfaI        
AflIII      AluI        Alw44I      AlwI        AlwNI       
AocI        ApaI        ApaLI       Asp718I     AspHI       
AspI        AvaI        AvaII       BalI        BanI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BbvI        BcgI        BcnI        BglI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaHI       BsaI        BsaJI       BsaWI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrBI       BsrFI       BsrI        BssKI       
BssSI       BstAPI      BstEII      BstF5I      BstNI       
BstPI       BstUI       BstYI       Bsu36I      Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DrdI        DsaI        EaeI        
EagI        EarI        EclXI       Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        KasI        
KpnI        Ksp632I     MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NaeI        NarI        NciI        NcoI        
NdeII       NgoMIV      NlaIII      NlaIV       NspI        
PflFI       PflMI       PleI        PmaCI       PmlI        
PpuMI       PstI        PvuII       RsaI        SacI        
SapI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SfoI        SmaI        SmlI        StuI        
StyI        TaqI        TseI        Tsp45I      TspRI       
Tth111I     TthHB8I     Van91I      XcmI        XhoII       
XmaCI       XmaI        
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