Restriction map for pF1KSDA1769
 (Linear) (Six-base) MAPSORT of: KIAA1769.seq  Check: 5962  from: 1  to: 6240



KIAA1769.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:29  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     931    3050    3273    3864    3933    4875    5320    6240 
   Size:        931    2119     223     591      69     942     445     920

  Fragments arranged by size: 

                2119     942     931     920     591     445     223      69
            
 
AatII G_ACGT'C

Cuts at:      0    2537    6240 
   Size:       2537    3703
 
AccI GT'mk_AC

Cuts at:      0    3689    4411    6240 
   Size:       3689     722    1829

  Fragments arranged by size: 

                3689    1829     722
 
Acc65I G'GTAC_C

Cuts at:      0    1676    3323    3981    4094    5531    6240 
   Size:       1676    1647     658     113    1437     709

  Fragments arranged by size: 

                1676    1647    1437     709     658     113
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1897    2005    2632    3405    4002    4174    4284    5029 
   Size:       1897     108     627     773     597     172     110     745

Cuts at:   5029    5644    6240 
   Size:        615     596

  Fragments arranged by size: 

                1897     773     745     627     615     597     596     172
                 110     108
 
AfeI AGC'GCT

Cuts at:      0    3223    3808    6240 
   Size:       3223     585    2432

  Fragments arranged by size: 

                3223    2432     585
 
AflIII A'CryG_T

Cuts at:      0     668    1919    3296    6240 
   Size:        668    1251    1377    2944

  Fragments arranged by size: 

                2944    1377    1251     668
 
AgeI A'CCGG_T

Cuts at:      0    4091    5513    6240 
   Size:       4091    1422     727

  Fragments arranged by size: 

                4091    1422     727
 
AleI CACnn'nnGTG

Cuts at:      0    2245    2413    2699    4199    6240 
   Size:       2245     168     286    1500    2041

  Fragments arranged by size: 

                2245    2041    1500     286     168
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2489    2717    3455    3979    5098    5155    6240 
   Size:       2489     228     738     524    1119      57    1085

  Fragments arranged by size: 

                2489    1119    1085     738     524     228      57
 
AlwNI CAG_nnn'CTG

Cuts at:      0     309     755     828     933    1115    1468    2168    2525 
   Size:        309     446      73     105     182     353     700     357

Cuts at:   2525    3354    3504    3786    4025    4197    4307    5329    5640 
   Size:        829     150     282     239     172     110    1022     311

Cuts at:   5640    6240 
   Size:        600

  Fragments arranged by size: 

                1022     829     700     600     446     357     353     311
                 309     282     239     182     172     150     110     105
                  73
 
ApaI G_GGCC'C

Cuts at:      0     420    1753    4083    4370    4816    6096    6240 
   Size:        420    1333    2330     287     446    1280     144

  Fragments arranged by size: 

                2330    1333    1280     446     420     287     144
 
ApaLI G'TGCA_C

Cuts at:      0     812     916    2401    2925    4489    6240 
   Size:        812     104    1485     524    1564    1751

  Fragments arranged by size: 

                1751    1564    1485     812     524     104
 
AvaI C'yCGr_G

Cuts at:      0      79     419    1120    1181    1399    1594    1669    1746 
   Size:         79     340     701      61     218     195      75      77

Cuts at:   1746    1840    2697    3245    3914    4083    4273    4327    4363 
   Size:         94     857     548     669     169     190      54      36

Cuts at:   4363    5099    6240 
   Size:        736    1141

  Fragments arranged by size: 

                1141     857     736     701     669     548     340     218
                 195     190     169      94      79      77      75      61
                  54      36
 
AvrII C'CTAG_G

Cuts at:      0     843    6240 
   Size:        843    5397
 
BamHI G'GATC_C

Cuts at:      0    3703    5150    6240 
   Size:       3703    1447    1090

  Fragments arranged by size: 

                3703    1447    1090
 
BanI G'GyrC_C

Cuts at:      0     212     258     352     799    1395    1676    1836    2214 
   Size:        212      46      94     447     596     281     160     378

Cuts at:   2214    2338    2701    2786    2833    3012    3323    3680    3955 
   Size:        124     363      85      47     179     311     357     275

Cuts at:   3955    3981    4008    4094    4246    4549    5531    6240 
   Size:         26      27      86     152     303     982     709

  Fragments arranged by size: 

                 982     709     596     447     378     363     357     311
                 303     281     275     212     179     160     152     124
                  94      86      85      47      46      27      26
 
BanII G_rGCy'C

Cuts at:      0       7     254     420    1312    1496    1670    1753    3379 
   Size:          7     247     166     892     184     174      83    1626

Cuts at:   3379    3410    3544    3991    4083    4370    4816    5098    5222 
   Size:         31     134     447      92     287     446     282     124

Cuts at:   5222    5837    6096    6107    6240 
   Size:        615     259      11     133

  Fragments arranged by size: 

                1626     892     615     447     446     287     282     259
                 247     184     174     166     134     133     124      92
                  83      31      11       7
 
BbeI G_GCGC'C

Cuts at:      0    1840    2342    3684    4250    6240 
   Size:       1840     502    1342     566    1990

  Fragments arranged by size: 

                1990    1840    1342     566     502
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     498    1394    4837    5632    6240 
   Size:        498     896    3443     795     608

  Fragments arranged by size: 

                3443     896     795     608     498
 
BbvCI CC'TCA_GC

Cuts at:      0     829    1454    2539    3992    4540    5039    5786    6240 
   Size:        829     625    1085    1453     548     499     747     454

  Fragments arranged by size: 

                1453    1085     829     747     625     548     499     454
            
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     342     376     768     802    2282    2316    4308    4342 
   Size:        342      34     392      34    1480      34    1992      34

Cuts at:   4342    6240 
   Size:       1898

  Fragments arranged by size: 

                1992    1898    1480     392     342      34      34      34
                  34
 
BclI T'GATC_A

Cuts at:      0    2684    6240 
   Size:       2684    3556
 
BfrBI ATG'CAT

Cuts at:      0    5574    6240 
   Size:       5574     666
 
BglI GCCn_nnn'nGGC

Cuts at:      0     424     885    1124    1411    1845    3374    3659    3893 
   Size:        424     461     239     287     434    1529     285     234

Cuts at:   3893    4233    5672    5920    6240 
   Size:        340    1439     248     320

  Fragments arranged by size: 

                1529    1439     461     434     424     340     320     287
                 285     248     239     234
 
BglII A'GATC_T

Cuts at:      0    1967    6240 
   Size:       1967    4273
 
BlpI GC'TnA_GC

Cuts at:      0     121     907     937    6179    6240 
   Size:        121     786      30    5242      61

  Fragments arranged by size: 

                5242     786     121      61      30
 
Bme1580I G_kGCm'C

Cuts at:      0     420     804     816     920     951    1400    1753    2219 
   Size:        420     384      12     104      31     449     353     466

Cuts at:   2219    2405    2432    2507    2929    3017    4083    4370    4493 
   Size:        186      27      75     422      88    1066     287     123

Cuts at:   4493    4552    4816    6096    6240 
   Size:         59     264    1280     144

  Fragments arranged by size: 

                1280    1066     466     449     422     420     384     353
                 287     264     186     144     123     104      88      75
                  59      31      27      12
 
BmgBI CAC'GTC

Cuts at:      0      63    1392    2451    3333    3531    4104    5352    5630 
   Size:         63    1329    1059     882     198     573    1248     278

Cuts at:   5630    6240 
   Size:        610

  Fragments arranged by size: 

                1329    1248    1059     882     610     573     278     198
                  63
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     130    1002    1221    4452    4666    5050    5648    6240 
   Size:        130     872     219    3231     214     384     598     592

  Fragments arranged by size: 

                3231     872     598     592     384     219     214     130
            
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    3558    4194    5236    5241    6142    6240 
   Size:       3558     636    1042       5     901      98

  Fragments arranged by size: 

                3558    1042     901     636      98       5
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     331    2218    3238    3412    3808    3925    4051    4165 
   Size:        331    1887    1020     174     396     117     126     114

Cuts at:   4165    4279    4729    4807    5211    5794    6240 
   Size:        114     450      78     404     583     446

  Fragments arranged by size: 

                1887    1020     583     450     446     404     396     331
                 174     126     117     114     114      78
 
Bpu10I CC'TnA_GC

Cuts at:      0     253     829    1454    2539    3992    4540    5039    5086 
   Size:        253     576     625    1085    1453     548     499      47

Cuts at:   5086    5786    6240 
   Size:        700     454

  Fragments arranged by size: 

                1453    1085     700     625     576     548     499     454
                 253      47
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2068    2229    2965    3475    6019    6240 
   Size:       2068     161     736     510    2544     221

  Fragments arranged by size: 

                2544    2068     736     510     221     161
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1696    2366    5447    5522    6113    6240 
   Size:       1696     670    3081      75     591     127

  Fragments arranged by size: 

                3081    1696     670     591     127      75
 
BsaAI yAC'GTr

Cuts at:      0     567     645    1922    3299    4038    4409    5706    6240 
   Size:        567      78    1277    1377     739     371    1297     534

  Fragments arranged by size: 

                1377    1297    1277     739     567     534     371      78
            
 
BsaHI Gr'CG_yC

Cuts at:      0     533     679     974    1034    1508    1717    1837    2339 
   Size:        533     146     295      60     474     209     120     502

Cuts at:   2339    2534    2741    3681    3850    4216    4225    4247    4630 
   Size:        195     207     940     169     366       9      22     383

Cuts at:   4630    6240 
   Size:       1610

  Fragments arranged by size: 

                1610     940     533     502     474     383     366     295
                 209     207     195     169     146     120      60      22
                   9
 
BsaWI w'CCGG_w

Cuts at:      0    2018    4091    4527    5147    5513    6240 
   Size:       2018    2073     436     620     366     727

  Fragments arranged by size: 

                2073    2018     727     620     436     366
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1364    1394    1726    1756    3876    3906    6240 
   Size:       1364      30     332      30    2120      30    2334

  Fragments arranged by size: 

                2334    2120    1364     332      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      80     846    1264    1734    2683    2692    4291    4639 
   Size:         80     766     418     470     949       9    1599     348

Cuts at:   4639    5658    5791    6240 
   Size:       1019     133     449

  Fragments arranged by size: 

                1599    1019     949     766     470     449     418     348
                 133      80       9
 
BseYI C'CCAG_C

Cuts at:      0     748     755    1013    1195    1311    1866    2130    3363 
   Size:        748       7     258     182     116     555     264    1233

Cuts at:   3363    3497    3513    3568    3854    3921    4369    4696    4756 
   Size:        134      16      55     286      67     448     327      60

Cuts at:   4756    4923    6240 
   Size:        167    1317

  Fragments arranged by size: 

                1317    1233     748     555     448     327     286     264
                 258     182     167     134     116      67      60      55
                  16       7
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     427     510     745    1834    3010    3028    3064    3457 
   Size:        427      83     235    1089    1176      18      36     393

Cuts at:   3457    3505    3892    3898    3967    4909    5125    5292    5342 
   Size:         48     387       6      69     942     216     167      50

Cuts at:   5342    5437    5728    6240 
   Size:         95     291     512

  Fragments arranged by size: 

                1176    1089     942     512     427     393     387     291
                 235     216     167      95      83      69      50      48
                  36      18       6
 
BsiEI CG_ry'CG

Cuts at:      0     118     171     266     428     765    2289    2709    3237 
   Size:        118      53      95     162     337    1524     420     528

Cuts at:   3237    3658    4838    6240 
   Size:        421    1180    1402

  Fragments arranged by size: 

                1524    1402    1180     528     421     420     337     162
                 118      95      53
 
BsiHKAI G_wGCw'C

Cuts at:      0       7     254     434     816     908     920     986    1496 
   Size:          7     247     180     382      92      12      66     510

Cuts at:   1496    2405    2929    3635    4037    4493    5222    5980    6240 
   Size:        909     524     706     402     456     729     758     260

  Fragments arranged by size: 

                 909     758     729     706     524     510     456     402
                 382     260     247     180      92      66      12       7
            
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1158    1367    3574    3757    6240 
   Size:       1158     209    2207     183    2483

  Fragments arranged by size: 

                2483    2207    1158     209     183
 
Bsp1286I G_dGCh'C

Cuts at:      0       7     254     420     434     804     816     908     920 
   Size:          7     247     166      14     370      12      92      12

Cuts at:    920     951     986    1312    1400    1496    1670    1753    2219 
   Size:         31      35     326      88      96     174      83     466

Cuts at:   2219    2405    2432    2507    2929    3017    3379    3410    3544 
   Size:        186      27      75     422      88     362      31     134

Cuts at:   3544    3635    3991    4037    4083    4370    4493    4552    4816 
   Size:         91     356      46      46     287     123      59     264

Cuts at:   4816    5098    5222    5837    5980    6096    6107    6240 
   Size:        282     124     615     143     116      11     133

  Fragments arranged by size: 

                 615     466     422     370     362     356     326     287
                 282     264     247     186     174     166     143     134
                 133     124     123     116      96      92      91      88
                  88      83      75      59      46      46      35      31
                  31      27      14      12      12      11       7
 
BspEI T'CCGG_A

Cuts at:      0    2018    4527    6240 
   Size:       2018    2509    1713

  Fragments arranged by size: 

                2509    2018    1713
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     375     699     931    1131    1637    1989    3050    3096 
   Size:        375     324     232     200     506     352    1061      46

Cuts at:   3096    3273    3311    3864    3933    4691    4875    5320    6240 
   Size:        177      38     553      69     758     184     445     920

  Fragments arranged by size: 

                1061     920     758     553     506     445     375     352
                 324     232     200     184     177      69      46      38
            
 
BsrBI CCG'CTC

Cuts at:      0     630    1104    1420    1674    1713    4044    4713    5490 
   Size:        630     474     316     254      39    2331     669     777

Cuts at:   5490    5529    5721    6240 
   Size:         39     192     519

  Fragments arranged by size: 

                2331     777     669     630     519     474     316     254
                 192      39      39
 
BsrFI r'CCGG_y

Cuts at:      0     113     261    2027    2078    2284    2514    2704    2718 
   Size:        113     148    1766      51     206     230     190      14

Cuts at:   2718    2783    3653    4091    4156    4466    4661    5288    5513 
   Size:         65     870     438      65     310     195     627     225

Cuts at:   5513    6199    6240 
   Size:        686      41

  Fragments arranged by size: 

                1766     870     686     627     438     310     230     225
                 206     195     190     148     113      65      65      51
                  41      14
 
BsrGI T'GTAC_A

Cuts at:      0     104    5024    6240 
   Size:        104    4920    1216

  Fragments arranged by size: 

                4920    1216     104
 
BssHII G'CGCG_C

Cuts at:      0    1659    3463    6240 
   Size:       1659    1804    2777

  Fragments arranged by size: 

                2777    1804    1659
 
BssSI C'ACGA_G

Cuts at:      0     361     478     511    1891    2896    5536    5716    6190 
   Size:        361     117      33    1380    1005    2640     180     474

Cuts at:   6190    6240 
   Size:         50

  Fragments arranged by size: 

                2640    1380    1005     474     361     180     117      50
                  33
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     438    2793    2924    3354    5329    6240 
   Size:        438    2355     131     430    1975     911

  Fragments arranged by size: 

                2355    1975     911     438     430     131
 
BstEII G'GTnAC_C

Cuts at:      0     225    1551    3156    4641    6240 
   Size:        225    1326    1605    1485    1599

  Fragments arranged by size: 

                1605    1599    1485    1326     225
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1731    5661    6240 
   Size:       1731    3930     579

  Fragments arranged by size: 

                3930    1731     579
 
BstYI r'GATC_y

Cuts at:      0     872    1967    3350    3703    5150    5360    6240 
   Size:        872    1095    1383     353    1447     210     880

  Fragments arranged by size: 

                1447    1383    1095     880     872     353     210
 
BstZ17I GTA'TAC

Cuts at:      0    4412    6240 
   Size:       4412    1828
 
Bsu36I CC'TnA_GG

Cuts at:      0    2156    5125    5641    6240 
   Size:       2156    2969     516     599

  Fragments arranged by size: 

                2969    2156     599     516
 
BtgI C'CryG_G

Cuts at:      0     674     795    1037    1133    1274    1445    1480    2588 
   Size:        674     121     242      96     141     171      35    1108

Cuts at:   2588    2636    2735    3161    3369    3758    4346    4425    4908 
   Size:         48      99     426     208     389     588      79     483

Cuts at:   4908    5387    5738    6240 
   Size:        479     351     502

  Fragments arranged by size: 

                1108     674     588     502     483     479     426     389
                 351     242     208     171     141     121      99      96
                  79      48      35
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1979    4635    5829    5895    6240 
   Size:       1979    2656    1194      66     345

  Fragments arranged by size: 

                2656    1979    1194     345      66
 
BtsI GCAGTG_nn'

Cuts at:      0      27    1385    1683    3022    3721    3933    6240 
   Size:         27    1358     298    1339     699     212    2307

  Fragments arranged by size: 

                2307    1358    1339     699     298     212      27
 
ClaI AT'CG_AT

Cuts at:      0     290    3401    6240 
   Size:        290    3111    2839

  Fragments arranged by size: 

                3111    2839     290
 
DraIII CAC_nnn'GTG

Cuts at:      0     219     483    6240 
   Size:        219     264    5757

  Fragments arranged by size: 

                5757     264     219
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     538    2746    5848    6240 
   Size:        538    2208    3102     392

  Fragments arranged by size: 

                3102    2208     538     392
 
EaeI y'GGCC_r

Cuts at:      0      72     115     410     425     569     762    1244    1271 
   Size:         72      43     295      15     144     193     482      27

Cuts at:   1271    1477    1976    2274    2286    2417    2516    2553    2567 
   Size:        206     499     298      12     131      99      37      14

Cuts at:   2567    2604    2706    2753    3033    3234    3267    3479    3626 
   Size:         37     102      47     280     201      33     212     147

Cuts at:   3626    3655    3755    3811    3894    4151    4349    5384    5700 
   Size:         29     100      56      83     257     198    1035     316

Cuts at:   5700    5921    6201    6240 
   Size:        221     280      39

  Fragments arranged by size: 

                1035     499     482     316     298     295     280     280
                 257     221     212     206     201     198     193     147
                 144     131     102     100      99      83      72      56
                  47      43      39      37      37      33      29      27
                  15      14      12
 
EagI C'GGCC_G

Cuts at:      0     115     425     762    2286    2706    3234    3655    6240 
   Size:        115     310     337    1524     420     528     421    2585

  Fragments arranged by size: 

                2585    1524     528     421     420     337     310     115
            
 
EarI CTCTTCn'nnn_

Cuts at:      0     457    1873    1879    2089    2608    3568    4501    4526 
   Size:        457    1416       6     210     519     960     933      25

Cuts at:   4526    5086    5227    6240 
   Size:        560     141    1013

  Fragments arranged by size: 

                1416    1013     960     933     560     519     457     210
                 141      25       6
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     172     596    2689    4243    6240 
   Size:        172     424    2093    1554    1997

  Fragments arranged by size: 

                2093    1997    1554     424     172
 
EcoICRI GAG'CTC

Cuts at:      0       5     252    1494    5220    6240 
   Size:          5     247    1242    3726    1020

  Fragments arranged by size: 

                3726    1242    1020     247       5
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     331    1897    2005    2218    2632    3238    3405    3412 
   Size:        331    1566     108     213     414     606     167       7

Cuts at:   3412    3808    3925    4002    4051    4165    4174    4279    4284 
   Size:        396     117      77      49     114       9     105       5

Cuts at:   4284    4729    4807    5029    5211    5644    5794    6240 
   Size:        445      78     222     182     433     150     446

  Fragments arranged by size: 

                1566     606     446     445     433     414     396     331
                 222     213     182     167     150     117     114     108
                 105      78      77      49       9       7       5
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     847    1181    1246    1606    2020    2527    3208    5776 
   Size:        847     334      65     360     414     507     681    2568

Cuts at:   5776    6240 
   Size:        464

  Fragments arranged by size: 

                2568     847     681     507     464     414     360     334
                  65
 
EcoO109I rG'GnC_Cy

Cuts at:      0     321     416     840    1058    1125    1239    1403    1750 
   Size:        321      95     424     218      67     114     164     347

Cuts at:   1750    1926    1989    2001    2178    2380    2985    3213    4080 
   Size:        176      63      12     177     202     605     228     867

Cuts at:   4080    4812    4900    4930    5160    5235    5637    5892    6240 
   Size:        732      88      30     230      75     402     255     348

  Fragments arranged by size: 

                 867     732     605     424     402     348     347     321
                 255     230     228     218     202     177     176     164
                 114      95      88      75      67      63      30      12
            
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    3534    5274    6240 
   Size:       3534    1740     966

  Fragments arranged by size: 

                3534    1740     966
 
FseI GG_CCGG'CC

Cuts at:      0    3657    4665    6240 
   Size:       3657    1008    1575

  Fragments arranged by size: 

                3657    1575    1008
 
FspI TGC'GCA

Cuts at:      0     707    6240 
   Size:        707    5533
 
HaeII r_GCGC'y

Cuts at:      0    1173    1840    2342    2474    3225    3364    3684    3810 
   Size:       1173     667     502     132     751     139     320     126

Cuts at:   3810    4250    4458    5964    6240 
   Size:        440     208    1506     276

  Fragments arranged by size: 

                1506    1173     751     667     502     440     320     276
                 208     139     132     126
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1114    1146    1702    1726    1729    1734    1758    1761 
   Size:       1114      32     556      24       3       5      24       3

Cuts at:   1761    4045    4077    4615    4647    5894    5926    6240 
   Size:       2284      32     538      32    1247      32     314

  Fragments arranged by size: 

                2284    1247    1114     556     538     314      32      32
                  32      32      24      24       5       3       3
 
HincII GTy'rAC

Cuts at:      0    4398    5370    6240 
   Size:       4398     972     870

  Fragments arranged by size: 

                4398     972     870
 
KasI G'GCGC_C

Cuts at:      0    1836    2338    3680    4246    6240 
   Size:       1836     502    1342     566    1994

  Fragments arranged by size: 

                1994    1836    1342     566     502
 
KpnI G_GTAC'C

Cuts at:      0    1680    3327    3985    4098    5535    6240 
   Size:       1680    1647     658     113    1437     705

  Fragments arranged by size: 

                1680    1647    1437     705     658     113
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2204    2825    4982    5159    6240 
   Size:       2204     621    2157     177    1081

  Fragments arranged by size: 

                2204    2157    1081     621     177
 
MscI TGG'CCA

Cuts at:      0     412     571    2419    2569    2606    3896    5386    5702 
   Size:        412     159    1848     150      37    1290    1490     316

Cuts at:   5702    6240 
   Size:        538

  Fragments arranged by size: 

                1848    1490    1290     538     412     316     159     150
                  37
 
MslI CAynn'nnrTG

Cuts at:      0     673    2245    2413    2699    2863    4199    4513    6240 
   Size:        673    1572     168     286     164    1336     314    1727

  Fragments arranged by size: 

                1727    1572    1336     673     314     286     168     164
            
 
MspA1I CmG'CkG

Cuts at:      0      68     120     333     524     752    1017    1204    1432 
   Size:         68      52     213     191     228     265     187     228

Cuts at:   1432    1731    2130    2610    2658    3239    3345    3456    3548 
   Size:        299     399     480      48     581     106     111      92

Cuts at:   3548    3624    3783    3858    3882    4239    4304    4638    5466 
   Size:         76     159      75      24     357      65     334     828

Cuts at:   5466    5811    6015    6240 
   Size:        345     204     225

  Fragments arranged by size: 

                 828     581     480     399     357     345     334     299
                 265     228     228     225     213     204     191     187
                 159     111     106      92      76      75      68      65
                  52      48      24
 
NaeI GCC'GGC

Cuts at:      0    2029    2080    2286    2516    2706    2720    2785    3655 
   Size:       2029      51     206     230     190      14      65     870

Cuts at:   3655    4663    6201    6240 
   Size:       1008    1538      39

  Fragments arranged by size: 

                2029    1538    1008     870     230     206     190      65
                  51      39      14
 
NarI GG'CG_CC

Cuts at:      0    1837    2339    3681    4247    6240 
   Size:       1837     502    1342     566    1993

  Fragments arranged by size: 

                1993    1837    1342     566     502
 
NcoI C'CATG_G

Cuts at:      0     674    1133    1274    2588    3369    6240 
   Size:        674     459     141    1314     781    2871

  Fragments arranged by size: 

                2871    1314     781     674     459     141
 
NdeI CA'TA_TG

Cuts at:      0    5549    6240 
   Size:       5549     691
 
NgoMIV G'CCGG_C

Cuts at:      0    2027    2078    2284    2514    2704    2718    2783    3653 
   Size:       2027      51     206     230     190      14      65     870

Cuts at:   3653    4661    6199    6240 
   Size:       1008    1538      41

  Fragments arranged by size: 

                2027    1538    1008     870     230     206     190      65
                  51      41      14
 
NsiI A_TGCA'T

Cuts at:      0    5576    6240 
   Size:       5576     664
 
NspI r_CATG'y

Cuts at:      0     384     672    3174    5574    6240 
   Size:        384     288    2502    2400     666

  Fragments arranged by size: 

                2502    2400     666     384     288
 
PasI CC'CwG_GG

Cuts at:      0     943    2334    2432    2838    5817    6240 
   Size:        943    1391      98     406    2979     423

  Fragments arranged by size: 

                2979    1391     943     423     406      98
 
PciI A'CATG_T

Cuts at:      0     668    6240 
   Size:        668    5572
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     309     755    1020    2168    2862    3504    3645    3727 
   Size:        309     446     265    1148     694     642     141      82

Cuts at:   3727    3903    4509    4767    6029    6240 
   Size:        176     606     258    1262     211

  Fragments arranged by size: 

                1262    1148     694     642     606     446     309     265
                 258     211     176     141      82
 
PfoI T'CCnGG_A

Cuts at:      0      41    2141    3833    3902    4706    5345    6161    6240 
   Size:         41    2100    1692      69     804     639     816      79

  Fragments arranged by size: 

                2100    1692     816     804     639      79      69      41
            
 
PmlI CAC'GTG

Cuts at:      0     645    1922    3299    4038    5706    6240 
   Size:        645    1277    1377     739    1668     534

  Fragments arranged by size: 

                1668    1377    1277     739     645     534
 
PpuMI rG'GwC_Cy

Cuts at:      0     840    1239    1926    1989    2001    2380    4930    5235 
   Size:        840     399     687      63      12     379    2550     305

Cuts at:   5235    6240 
   Size:       1005

  Fragments arranged by size: 

                2550    1005     840     687     399     379     305      63
                  12
 
PshAI GACnn'nnGTC

Cuts at:      0    2378    6240 
   Size:       2378    3862
 
PspOMI G'GGCC_C

Cuts at:      0     416    1749    4079    4366    4812    6092    6240 
   Size:        416    1333    2330     287     446    1280     148

  Fragments arranged by size: 

                2330    1333    1280     446     416     287     148
 
PspXI vC'TCGA_Gb

Cuts at:      0    4273    4327    6240 
   Size:       4273      54    1913

  Fragments arranged by size: 

                4273    1913      54
 
PstI C_TGCA'G

Cuts at:      0     149    1431    1607    2036    2255    3195    3284    3623 
   Size:        149    1282     176     429     219     940      89     339

Cuts at:   3623    3806    3944    3968    4244    4687    5210    6220    6240 
   Size:        183     138      24     276     443     523    1010      20

  Fragments arranged by size: 

                1282    1010     940     523     443     429     339     276
                 219     183     176     149     138      89      24      20
            
 
PvuI CG_AT'CG

Cuts at:      0    4838    6240 
   Size:       4838    1402
 
PvuII CAG'CTG

Cuts at:      0     752    1017    2610    2658    3624    3783    4239    4304 
   Size:        752     265    1593      48     966     159     456      65

Cuts at:   4304    5466    5811    6240 
   Size:       1162     345     429

  Fragments arranged by size: 

                1593    1162     966     752     456     429     345     265
                 159      65      48
 
RsrII CG'GwC_CG

Cuts at:      0     111    2798    6240 
   Size:        111    2687    3442

  Fragments arranged by size: 

                3442    2687     111
 
SacI G_AGCT'C

Cuts at:      0       7     254    1496    5222    6240 
   Size:          7     247    1242    3726    1018

  Fragments arranged by size: 

                3726    1242    1018     247       7
 
SanDI GG'GwC_CC

Cuts at:      0    1926    2380    4930    6240 
   Size:       1926     454    2550    1310

  Fragments arranged by size: 

                2550    1926    1310     454
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2089    2608    3568    5086    5227    6240 
   Size:       2089     519     960    1518     141    1013

  Fragments arranged by size: 

                2089    1518    1013     960     519     141
 
SbfI CC_TGCA'GG

Cuts at:      0    3195    3944    6240 
   Size:       3195     749    2296

  Fragments arranged by size: 

                3195    2296     749
 
ScaI AGT'ACT

Cuts at:      0     151    6240 
   Size:        151    6089
 
SexAI A'CCwGG_T

Cuts at:      0    4818    5237    6240 
   Size:       4818     419    1003

  Fragments arranged by size: 

                4818    1003     419
 
SfcI C'TryA_G

Cuts at:      0     145    1427    1603    2010    2032    2251    3191    3280 
   Size:        145    1282     176     407      22     219     940      89

Cuts at:   3280    3619    3802    3940    3964    4240    4683    5206    6216 
   Size:        339     183     138      24     276     443     523    1010

Cuts at:   6216    6240 
   Size:         24

  Fragments arranged by size: 

                1282    1010     940     523     443     407     339     276
                 219     183     176     145     138      89      24      24
                  22
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     424    3659    6240 
   Size:        424    3235    2581

  Fragments arranged by size: 

                3235    2581     424
 
SfoI GGC'GCC

Cuts at:      0    1838    2340    3682    4248    6240 
   Size:       1838     502    1342     566    1992

  Fragments arranged by size: 

                1992    1838    1342     566     502
 
SgrAI Cr'CCGG_yG

Cuts at:      0    4156    6240 
   Size:       4156    2084
 
SmaI CCC'GGG

Cuts at:      0     421    1401    2699    3247    3916    4365    5101    6240 
   Size:        421     980    1298     548     669     449     736    1139

  Fragments arranged by size: 

                1298    1139     980     736     669     548     449     421
            
 
SmlI C'TyrA_G

Cuts at:      0    2047    2208    2980    3490    4273    4327    6034    6240 
   Size:       2047     161     772     510     783      54    1707     206

  Fragments arranged by size: 

                2047    1707     783     772     510     206     161      54
            
 
SphI G_CATG'C

Cuts at:      0    5574    6240 
   Size:       5574     666
 
SrfI GCCC'GGGC

Cuts at:      0     421    6240 
   Size:        421    5819
 
StuI AGG'CCT

Cuts at:      0     733    3418    5191    5875    6240 
   Size:        733    2685    1773     684     365

  Fragments arranged by size: 

                2685    1773     733     684     365
 
StyI C'CwwG_G

Cuts at:      0     572     674     843    1133    1274    2243    2588    3369 
   Size:        572     102     169     290     141     969     345     781

Cuts at:   3369    5762    6240 
   Size:       2393     478

  Fragments arranged by size: 

                2393     969     781     572     478     345     290     169
                 141     102
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     178    3142    3191    4407    6240 
   Size:        178    2964      49    1216    1833

  Fragments arranged by size: 

                2964    1833    1216     178      49
 
TatI w'GTAC_w

Cuts at:      0     104     149    2201    2873    4943    5024    6240 
   Size:        104      45    2052     672    2070      81    1216

  Fragments arranged by size: 

                2070    2052    1216     672     104      81      45
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1364    1396    3876    3908    6240 
   Size:       1364      32    2480      32    2332

  Fragments arranged by size: 

                2480    2332    1364      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     136    1582    3154    4180    5239    6240 
   Size:        136    1446    1572    1026    1059    1001

  Fragments arranged by size: 

                1572    1446    1059    1026    1001     136
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1971    2269    2391    4653    4704    4764    4965    5235 
   Size:       1971     298     122    2262      51      60     201     270

Cuts at:   5235    5769    6003    6240 
   Size:        534     234     237

  Fragments arranged by size: 

                2262    1971     534     298     270     237     234     201
                 122      60      51
 
XhoI C'TCGA_G

Cuts at:      0    4273    4327    6240 
   Size:       4273      54    1913

  Fragments arranged by size: 

                4273    1913      54
 
XmaI C'CCGG_G

Cuts at:      0     419    1399    2697    3245    3914    4363    5099    6240 
   Size:        419     980    1298     548     669     449     736    1141

  Fragments arranged by size: 

                1298    1141     980     736     669     548     449     419
            
 
XmnI GAAnn'nnTTC

Cuts at:      0    4795    6240 
   Size:       4795    1445
 
ZraI GAC'GTC

Cuts at:      0    2535    6240 
   Size:       2535    3705
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AcuI     AfeI   AflIII     AgeI
     AleI     AlfI    AlwNI     ApaI    ApaLI     AvaI    AvrII    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspMI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I     FalI     FseI     FspI    HaeII    Hin4I   HincII     KasI
     KpnI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NsiI     NspI     PasI     PciI    PflMI     PfoI
     PmlI    PpuMI    PshAI   PspOMI    PspXI     PstI     PvuI    PvuII
    RsrII     SacI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI    SgrAI     SmaI     SmlI     SphI     SrfI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AclI    AflII     AhdI     AloI     ApoI     AscI     AseI    AsiSI
     BaeI    BciVI     BmtI    BsaBI    BsiWI     BsmI    BspHI    BsrDI
    BstBI    CspCI     DraI    EcoRI    EcoRV    FspAI  HindIII     HpaI
     MfeI     MluI     NheI     NotI     NruI     PacI     PmeI     PpiI
     PsiI     PsrI    SacII     SalI     SgfI    SnaBI     SpeI     SspI
     SwaI     XbaI
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