Restriction map for
pF1KSDA1734
(Circular) (Six-base) MAPSORT of: pF1KSDA1734.seq Check: 427 from: 1 to: 7013
pF1KSDA1734 7013 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AatII G_ACGT'C
Cuts at: 632 854 2183 632
Size: 222 1329 5462
Fragments arranged by size:
5462 1329 222
AccI GT'mk_AC
Cuts at: 764 1934 4051 764
Size: 1170 2117 3726
Fragments arranged by size:
3726 2117 1170
Acc65I G'GTAC_C
Cuts at: 503 4029 503
Size: 3526 3487
AclI AA'CG_TT
Cuts at: 6853 6853
Size: 7013
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 811 2973 4838 5270 6017 6285 811
Size: 2162 1865 432 747 268 1539
Fragments arranged by size:
2162 1865 1539 747 432 268
AfeI AGC'GCT
Cuts at: 1180 1180
Size: 7013
AflIII A'CryG_T
Cuts at: 4365 5469 6446 4365
Size: 1104 977 4932
Fragments arranged by size:
4932 1104 977
AgeI A'CCGG_T
Cuts at: 5401 5401
Size: 7013
AhdI GACnn_n'nnGTC
Cuts at: 321 321
Size: 7013
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 715 715
Size: 7013
AlwNI CAG_nnn'CTG
Cuts at: 300 960 1927 2622 3419 3597 3747 4318 5885
Size: 660 967 695 797 178 150 571 1567
Cuts at: 5885 300
Size: 1428
Fragments arranged by size:
1567 1428 967 797 695 660 571 178
150
ApaI G_GGCC'C
Cuts at: 3100 3100
Size: 7013
ApaLI G'TGCA_C
Cuts at: 346 1852 3987 5783 346
Size: 1506 2135 1796 1576
Fragments arranged by size:
2135 1796 1576 1506
ApoI r'AATT_y
Cuts at: 4017 6366 6622 4017
Size: 2349 256 4408
Fragments arranged by size:
4408 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7013
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7013
AvaI C'yCGr_G
Cuts at: 420 572 873 1346 1730 2669 2800 3484 4033
Size: 152 301 473 384 939 131 684 549
Cuts at: 4033 420
Size: 3400
Fragments arranged by size:
3400 939 684 549 473 384 301 152
131
AvrII C'CTAG_G
Cuts at: 1122 1122
Size: 7013
BamHI G'GATC_C
Cuts at: 132 4038 132
Size: 3906 3107
BanI G'GyrC_C
Cuts at: 228 234 503 567 661 805 835 3259 3609
Size: 6 269 64 94 144 30 2424 350
Cuts at: 3609 3736 3807 4029 4443 4691 4726 228
Size: 127 71 222 414 248 35 2515
Fragments arranged by size:
2515 2424 414 350 269 248 222 144
127 94 71 64 35 30 6
BanII G_rGCy'C
Cuts at: 421 1267 1484 2133 2399 2632 3100 3638 3721
Size: 846 217 649 266 233 468 538 83
Cuts at: 3721 4027 5057 421
Size: 306 1030 2377
Fragments arranged by size:
2377 1030 846 649 538 468 306 266
233 217 83
BbeI G_GCGC'C
Cuts at: 232 238 809 839 4447 4695 232
Size: 6 571 30 3608 248 2550
Fragments arranged by size:
3608 2550 571 248 30 6
BbsI GAAGACnn'nnnn_
Cuts at: 1185 1370 2728 2901 1185
Size: 185 1358 173 5297
Fragments arranged by size:
5297 1358 185 173
BbvCI CC'TCA_GC
Cuts at: 1785 1996 3753 1785
Size: 211 1757 5045
Fragments arranged by size:
5045 1757 211
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 606 640 1423 1457 606
Size: 34 783 34 6162
Fragments arranged by size:
6162 783 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4911 5672 7013 4911
Size: 761 1341 4911
Fragments arranged by size:
4911 1341 761
BclI T'GATC_A
Cuts at: 2450 4533 2450
Size: 2083 4930
BglI GCCn_nnn'nGGC
Cuts at: 193 2509 2805 3257 193
Size: 2316 296 452 3949
Fragments arranged by size:
3949 2316 452 296
BglII A'GATC_T
Cuts at: 938 4528 6565 938
Size: 3590 2037 1386
Fragments arranged by size:
3590 2037 1386
BlpI GC'TnA_GC
Cuts at: 982 1160 4125 982
Size: 178 2965 3870
Fragments arranged by size:
3870 2965 178
Bme1580I G_kGCm'C
Cuts at: 350 572 1078 1856 2774 3100 3173 3262 3614
Size: 222 506 778 918 326 73 89 352
Cuts at: 3614 3741 3991 4638 4731 5787 350
Size: 127 250 647 93 1056 1576
Fragments arranged by size:
1576 1056 918 778 647 506 352 326
250 222 127 93 89 73
BmgBI CAC'GTC
Cuts at: 864 1794 2016 864
Size: 930 222 5861
Fragments arranged by size:
5861 930 222
BmrI ACTGGGnnnn_n'
Cuts at: 1332 3809 4225 4306 4641 6785 1332
Size: 2477 416 81 335 2144 1560
Fragments arranged by size:
2477 2144 1560 416 335 81
BmtI G_CTAG'C
Cuts at: 3761 3761
Size: 7013
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2928 5380 2928
Size: 2452 4561
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 149 167 930 1904 2434 2608 2941 2954 3295
Size: 18 763 974 530 174 333 13 341
Cuts at: 3295 3601 149
Size: 306 3561
Fragments arranged by size:
3561 974 763 530 341 333 306 174
18 13
Bpu10I CC'TnA_GC
Cuts at: 1785 1996 3753 1785
Size: 211 1757 5045
Fragments arranged by size:
5045 1757 211
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2581 2701 4189 5068 5560 5858 2581
Size: 120 1488 879 492 298 3736
Fragments arranged by size:
3736 1488 879 492 298 120
BsaI GGTCTCn'nnnn_
Cuts at: 529 883 3781 6717 529
Size: 354 2898 2936 825
Fragments arranged by size:
2936 2898 825 354
BsaAI yAC'GTr
Cuts at: 489 4005 4366 489
Size: 3516 361 3136
Fragments arranged by size:
3516 3136 361
BsaBI GATnn'nnATC
Cuts at: 6 4527 6564 6
Size: 4521 2037 455
Fragments arranged by size:
4521 2037 455
BsaHI Gr'CG_yC
Cuts at: 229 235 515 629 806 836 851 1429 2180
Size: 6 280 114 177 30 15 578 751
Cuts at: 2180 2879 4444 4692 5394 6890 229
Size: 699 1565 248 702 1496 352
Fragments arranged by size:
1565 1496 751 702 699 578 352 280
248 177 114 30 15 6
BsaWI w'CCGG_w
Cuts at: 3848 4202 4723 5401 5675 5822 6233 3848
Size: 354 521 678 274 147 411 4628
Fragments arranged by size:
4628 678 521 411 354 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 238 268 325 355 1816 1846 1877 1907 2188
Size: 30 57 30 1461 30 31 30 281
Cuts at: 2188 2218 2665 2695 238
Size: 30 447 30 4556
Fragments arranged by size:
4556 1461 447 281 57 31 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 415 418 469 1438 1492 1602 2123 2284 2407
Size: 3 51 969 54 110 521 161 123
Cuts at: 2407 2764 2767 415
Size: 357 3 4661
Fragments arranged by size:
4661 969 521 357 161 123 110 54
51 3 3
BseYI C'CCAG_C
Cuts at: 207 753 1197 2237 2345 2833 3162 3464 3639
Size: 546 444 1040 108 488 329 302 175
Cuts at: 3639 4438 5196 5773 6269 207
Size: 799 758 577 496 951
Fragments arranged by size:
1040 951 799 758 577 546 496 488
444 329 302 175 108
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1042 1493 1528 3472 1042
Size: 451 35 1944 4583
Fragments arranged by size:
4583 1944 451 35
BsiEI CG_ry'CG
Cuts at: 104 1968 4555 4601 5809 104
Size: 1864 2587 46 1208 1308
Fragments arranged by size:
2587 1864 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 350 421 430 817 923 983 1856 2381 3320
Size: 71 9 387 106 60 873 525 939
Cuts at: 3320 3991 4027 4805 4995 5787 350
Size: 671 36 778 190 792 1576
Fragments arranged by size:
1576 939 873 792 778 671 525 387
190 106 71 60 36 9
BsmI GAATG_Cn'
Cuts at: 1825 1825
Size: 7013
BsmBI CGTCTCn'nnnn_
Cuts at: 1144 1420 2344 1144
Size: 276 924 5813
Fragments arranged by size:
5813 924 276
Bsp1286I G_dGCh'C
Cuts at: 350 421 430 572 817 923 983 1078 1267
Size: 71 9 142 245 106 60 95 189
Cuts at: 1267 1484 1856 2133 2381 2399 2632 2774 3100
Size: 217 372 277 248 18 233 142 326
Cuts at: 3100 3173 3262 3320 3614 3638 3721 3741 3991
Size: 73 89 58 294 24 83 20 250
Cuts at: 3991 4027 4638 4731 4805 4995 5057 5787 350
Size: 36 611 93 74 190 62 730 1576
Fragments arranged by size:
1576 730 611 372 326 294 277 250
248 245 233 217 190 189 142 142
106 95 93 89 83 74 73 71
62 60 58 36 24 20 18 9
BspEI T'CCGG_A
Cuts at: 6233 6233
Size: 7013
BspHI T'CATG_A
Cuts at: 6189 6189
Size: 7013
BspMI ACCTGCnnnn'nnnn_
Cuts at: 707 1014 1293 1981 4063 4277 4579 4960 707
Size: 307 279 688 2082 214 302 381 2760
Fragments arranged by size:
2760 2082 688 381 307 302 279 214
BsrBI CCG'CTC
Cuts at: 50 163 1643 1832 5305 5359 6843 7008 50
Size: 113 1480 189 3473 54 1484 165 55
Fragments arranged by size:
3473 1484 1480 189 165 113 55 54
BsrDI GCAATG_nn'
Cuts at: 734 2057 4925 734
Size: 1323 2868 2822
Fragments arranged by size:
2868 2822 1323
BsrFI r'CCGG_y
Cuts at: 231 286 2421 3370 5011 5192 5401 231
Size: 55 2135 949 1641 181 209 1843
Fragments arranged by size:
2135 1843 1641 949 209 181 55
BsrGI T'GTAC_A
Cuts at: 2246 2246
Size: 7013
BssSI C'ACGA_G
Cuts at: 451 1486 1653 5284 5642 451
Size: 1035 167 3631 358 1822
Fragments arranged by size:
3631 1822 1035 358 167
BstAPI GCAn_nnn'nTGC
Cuts at: 300 668 1308 4390 300
Size: 368 640 3082 2923
Fragments arranged by size:
3082 2923 640 368
BstBI TT'CG_AA
Cuts at: 110 5374 110
Size: 5264 1749
BstEII G'GTnAC_C
Cuts at: 1605 1770 1605
Size: 165 6848
BstXI CCAn_nnnn'nTGG
Cuts at: 1452 2863 3202 1452
Size: 1411 339 5263
Fragments arranged by size:
5263 1411 339
BstYI r'GATC_y
Cuts at: 1 132 938 2582 4038 4505 4528 4863 5109
Size: 131 806 1644 1456 467 23 335 246
Cuts at: 5109 6121 6207 6219 6565 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1644 1456 1012 806 467 449 346 335
246 131 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1421 2432 3035 3680 1421
Size: 1011 603 645 4754
Fragments arranged by size:
4754 1011 645 603
BtgI C'CryG_G
Cuts at: 125 336 372 411 480 1358 1814 2495 2507
Size: 211 36 39 69 878 456 681 12
Cuts at: 2507 125
Size: 4631
Fragments arranged by size:
4631 878 681 456 211 69 39 36
12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4963 5144 5307 4963
Size: 181 163 6669
Fragments arranged by size:
6669 181 163
BtsI GCAGTG_nn'
Cuts at: 1766 2774 2866 3028 3207 3595 4256 1766
Size: 1008 92 162 179 388 661 4523
Fragments arranged by size:
4523 1008 661 388 179 162 92
DraI TTT'AAA
Cuts at: 4012 4012
Size: 7013
DraIII CAC_nnn'GTG
Cuts at: 1340 3817 1340
Size: 2477 4536
DrdI GACnn_nn'nnGTC
Cuts at: 646 1930 3297 4719 5577 646
Size: 1284 1367 1422 858 2082
Fragments arranged by size:
2082 1422 1367 1284 858
EaeI y'GGCC_r
Cuts at: 1271 1965 2492 2510 2738 3204 3666 4598 4772
Size: 694 527 18 228 466 462 932 174
Cuts at: 4772 5163 5190 6535 1271
Size: 391 27 1345 1749
Fragments arranged by size:
1749 1345 932 694 527 466 462 391
228 174 27 18
EagI C'GGCC_G
Cuts at: 1965 4598 1965
Size: 2633 4380
EarI CTCTTCn'nnn_
Cuts at: 886 1010 1849 2027 2453 3082 3862 3904 5036
Size: 124 839 178 426 629 780 42 1132
Cuts at: 5036 5246 886
Size: 210 2653
Fragments arranged by size:
2653 1132 839 780 629 426 210 178
124 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 145 5221 5531 5677 6823 145
Size: 5076 310 146 1146 335
Fragments arranged by size:
5076 1146 335 310 146
EcoICRI GAG'CTC
Cuts at: 419 4025 419
Size: 3606 3407
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 149 167 811 930 1904 2434 2608 2941 2954
Size: 18 644 119 974 530 174 333 13
Cuts at: 2954 2973 3295 3601 4838 5270 6017 6285 149
Size: 19 322 306 1237 432 747 268 877
Fragments arranged by size:
1237 974 877 747 644 530 432 333
322 306 268 174 119 19 18 13
EcoNI CCTnn'n_nnAGG
Cuts at: 3310 3967 3310
Size: 657 6356
EcoO109I rG'GnC_Cy
Cuts at: 174 696 3096 3097 4152 174
Size: 522 2400 1 1055 3035
Fragments arranged by size:
3035 2400 1055 522 1
EcoRI G'AATT_C
Cuts at: 4017 4017
Size: 7013
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3003 5032 3003
Size: 2029 4984
FspI TGC'GCA
Cuts at: 4794 4794
Size: 7013
HaeII r_GCGC'y
Cuts at: 232 238 300 809 839 1182 1290 2897 3016
Size: 6 62 509 30 343 108 1607 119
Cuts at: 3016 3439 4447 4695 5717 6696 232
Size: 423 1008 248 1022 979 549
Fragments arranged by size:
1607 1022 1008 979 549 509 423 343
248 119 108 62 30 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 238 270 307 339 373 405 566 598 1050
Size: 32 37 32 34 32 161 32 452
Cuts at: 1050 1082 2137 2169 2435 2467 2794 2826 3064
Size: 32 1055 32 266 32 327 32 238
Cuts at: 3064 3096 4855 4885 4887 4917 238
Size: 32 1759 30 2 30 2334
Fragments arranged by size:
2334 1759 1055 452 327 266 238 161
37 34 32 32 32 32 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 633 687 4052 633
Size: 54 3365 3594
Fragments arranged by size:
3594 3365 54
KasI G'GCGC_C
Cuts at: 228 234 805 835 4443 4691 228
Size: 6 571 30 3608 248 2550
Fragments arranged by size:
3608 2550 571 248 30 6
KpnI G_GTAC'C
Cuts at: 507 4033 507
Size: 3526 3487
MluI A'CGCG_T
Cuts at: 6446 6446
Size: 7013
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 824 1859 2668 5684 5868 824
Size: 1035 809 3016 184 1969
Fragments arranged by size:
3016 1969 1035 809 184
MscI TGG'CCA
Cuts at: 1273 2494 2740 4774 1273
Size: 1221 246 2034 3512
Fragments arranged by size:
3512 2034 1221 246
MslI CAynn'nnrTG
Cuts at: 2608 5002 5129 2608
Size: 2394 127 4492
Fragments arranged by size:
4492 2394 127
MspA1I CmG'CkG
Cuts at: 413 482 1164 1503 1649 2306 2345 2473 2619
Size: 69 682 339 146 657 39 128 146
Cuts at: 2619 3468 3643 4124 4438 4798 5811 6056 413
Size: 849 175 481 314 360 1013 245 1370
Fragments arranged by size:
1370 1013 849 682 657 481 360 339
314 245 175 146 146 128 69 39
NaeI GCC'GGC
Cuts at: 233 288 3372 5194 233
Size: 55 3084 1822 2052
Fragments arranged by size:
3084 2052 1822 55
NarI GG'CG_CC
Cuts at: 229 235 806 836 4444 4692 229
Size: 6 571 30 3608 248 2550
Fragments arranged by size:
3608 2550 571 248 30 6
NcoI C'CATG_G
Cuts at: 125 1358 2495 2507 125
Size: 1233 1137 12 4631
Fragments arranged by size:
4631 1233 1137 12
NgoMIV G'CCGG_C
Cuts at: 231 286 3370 5192 231
Size: 55 3084 1822 2052
Fragments arranged by size:
3084 2052 1822 55
NheI G'CTAG_C
Cuts at: 3757 3757
Size: 7013
NspI r_CATG'y
Cuts at: 529 594 3981 4066 4566 5473 529
Size: 65 3387 85 500 907 2069
Fragments arranged by size:
3387 2069 907 500 85 65
PasI CC'CwG_GG
Cuts at: 1254 3714 1254
Size: 2460 4553
PciI A'CATG_T
Cuts at: 5469 5469
Size: 7013
PflMI CCAn_nnn'nTGG
Cuts at: 1359 3747 1359
Size: 2388 4625
PfoI T'CCnGG_A
Cuts at: 2558 2585 2558
Size: 27 6986
PmeI GTTT'AAAC
Cuts at: 4012 4012
Size: 7013
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6152 6184 6152
Size: 32 6981
PpuMI rG'GwC_Cy
Cuts at: 696 696
Size: 7013
PshAI GACnn'nnGTC
Cuts at: 875 875
Size: 7013
PspOMI G'GGCC_C
Cuts at: 3096 3096
Size: 7013
PstI C_TGCA'G
Cuts at: 704 3398 4060 4745 704
Size: 2694 662 685 2972
Fragments arranged by size:
2972 2694 685 662
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7013
PvuII CAG'CTG
Cuts at: 1503 2345 2473 2619 3468 3643 4438 4798 1503
Size: 842 128 146 849 175 795 360 3718
Fragments arranged by size:
3718 849 842 795 360 175 146 128
RsrII CG'GwC_CG
Cuts at: 5208 5208
Size: 7013
SacI G_AGCT'C
Cuts at: 421 4027 421
Size: 3606 3407
SacII CC_GC'GG
Cuts at: 414 483 414
Size: 69 6944
SalI G'TCGA_C
Cuts at: 4050 4050
Size: 7013
SapI GCTCTTCn'nnn_
Cuts at: 5036 5246 5036
Size: 210 6803
SbfI CC_TGCA'GG
Cuts at: 3398 4060 3398
Size: 662 6351
ScaI AGT'ACT
Cuts at: 6530 6530
Size: 7013
SexAI A'CCwGG_T
Cuts at: 317 317
Size: 7013
SfcI C'TryA_G
Cuts at: 33 700 2704 3394 3644 4056 4741 5734 5925
Size: 667 2004 690 250 412 685 993 191
Cuts at: 5925 33
Size: 1121
Fragments arranged by size:
2004 1121 993 690 685 667 412 250
191
SfoI GGC'GCC
Cuts at: 230 236 807 837 4445 4693 230
Size: 6 571 30 3608 248 2550
Fragments arranged by size:
3608 2550 571 248 30 6
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7013
SgrAI Cr'CCGG_yG
Cuts at: 231 231
Size: 7013
SmaI CCC'GGG
Cuts at: 1348 4035 1348
Size: 2687 4326
SmlI C'TyrA_G
Cuts at: 1730 2596 2716 4168 5083 5575 5837 1730
Size: 866 120 1452 915 492 262 2906
Fragments arranged by size:
2906 1452 915 866 492 262 120
SnaBI TAC'GTA
Cuts at: 4005 4005
Size: 7013
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7013
SphI G_CATG'C
Cuts at: 3981 4066 4566 3981
Size: 85 500 6428
Fragments arranged by size:
6428 500 85
StuI AGG'CCT
Cuts at: 3392 3709 3392
Size: 317 6696
StyI C'CwwG_G
Cuts at: 125 1122 1202 1358 2495 2507 2897 3330 3614
Size: 997 80 156 1137 12 390 433 284
Cuts at: 3614 3911 4147 4501 125
Size: 297 236 354 2637
Fragments arranged by size:
2637 1137 997 433 390 354 297 284
236 156 80 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1126 1351 2562 3401 3828 4596 4729 5188 5397
Size: 225 1211 839 427 768 133 459 209
Cuts at: 5397 1126
Size: 2742
Fragments arranged by size:
2742 1211 839 768 459 427 225 209
133
TatI w'GTAC_w
Cuts at: 2246 6528 2246
Size: 4282 2731
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 580 612 1877 1909 2188 2220 3228 3260 6530
Size: 32 1265 32 279 32 1008 32 3270
Cuts at: 6530 6562 580
Size: 32 1031
Fragments arranged by size:
3270 1265 1031 1008 279 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1084 2098 4810 1084
Size: 1014 2712 3287
Fragments arranged by size:
3287 2712 1014
XbaI T'CTAG_A
Cuts at: 4044 4044
Size: 7013
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 922 2409 2583 3465 4508 922
Size: 1487 174 882 1043 3427
Fragments arranged by size:
3427 1487 1043 882 174
XhoI C'TCGA_G
Cuts at: 1730 1730
Size: 7013
XmaI C'CCGG_G
Cuts at: 1346 4033 1346
Size: 2687 4326
XmnI GAAnn'nnTTC
Cuts at: 1987 2986 1987
Size: 999 6014
ZraI GAC'GTC
Cuts at: 630 852 2181 630
Size: 222 1329 5462
Fragments arranged by size:
5462 1329 222
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AflII AleI AloI AscI BaeI BfrBI BsiWI
BssHII BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NotI NruI NsiI PacI PmlI
PsiI PspXI PsrI SanDI SfiI SrfI SspI SwaI