Restriction map for
pF1KSDA1219
(Linear) (Six-base) MAPSORT of: KIAA1219.seq Check: 7409 from: 1 to: 4473
KIAA1219.orf
With 182 enzymes: SgfI *
February 28, 2007 11:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 117 4473
Size: 117 4356
AatII G_ACGT'C
Cuts at: 0 1240 4473
Size: 1240 3233
AccI GT'mk_AC
Cuts at: 0 3378 4473
Size: 3378 1095
Acc65I G'GTAC_C
Cuts at: 0 136 4473
Size: 136 4337
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 128 1993 2155 2705 3095 3164 3376 3722
Size: 128 1865 162 550 390 69 212 346
Cuts at: 3722 4473
Size: 751
Fragments arranged by size:
1865 751 550 390 346 212 162 128
69
AflII C'TTAA_G
Cuts at: 0 2232 2557 4473
Size: 2232 325 1916
Fragments arranged by size:
2232 1916 325
AflIII A'CryG_T
Cuts at: 0 863 4473
Size: 863 3610
AlwNI CAG_nnn'CTG
Cuts at: 0 151 432 450 2254 4473
Size: 151 281 18 1804 2219
Fragments arranged by size:
2219 1804 281 151 18
ApaLI G'TGCA_C
Cuts at: 0 725 2418 4473
Size: 725 1693 2055
Fragments arranged by size:
2055 1693 725
ApoI r'AATT_y
Cuts at: 0 162 919 1510 1798 3298 3568 3872 4205
Size: 162 757 591 288 1500 270 304 333
Cuts at: 4205 4225 4473
Size: 20 248
Fragments arranged by size:
1500 757 591 333 304 288 270 248
162 20
AseI AT'TA_AT
Cuts at: 0 1844 1996 3650 4473
Size: 1844 152 1654 823
Fragments arranged by size:
1844 1654 823 152
AvaI C'yCGr_G
Cuts at: 0 476 4473
Size: 476 3997
BanI G'GyrC_C
Cuts at: 0 136 1154 2002 2309 4473
Size: 136 1018 848 307 2164
Fragments arranged by size:
2164 1018 848 307 136
BanII G_rGCy'C
Cuts at: 0 2195 2261 2348 4278 4473
Size: 2195 66 87 1930 195
Fragments arranged by size:
2195 1930 195 87 66
BbeI G_GCGC'C
Cuts at: 0 2313 4473
Size: 2313 2160
BbsI GAAGACnn'nnnn_
Cuts at: 0 1195 1588 2943 3530 4473
Size: 1195 393 1355 587 943
Fragments arranged by size:
1355 1195 943 587 393
BbvCI CC'TCA_GC
Cuts at: 0 1541 4473
Size: 1541 2932
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 778 812 2262 2296 4473
Size: 778 34 1450 34 2177
Fragments arranged by size:
2177 1450 778 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 983 4473
Size: 983 3490
BclI T'GATC_A
Cuts at: 0 42 4473
Size: 42 4431
BfrBI ATG'CAT
Cuts at: 0 1042 2702 4473
Size: 1042 1660 1771
Fragments arranged by size:
1771 1660 1042
BglII A'GATC_T
Cuts at: 0 3108 4132 4473
Size: 3108 1024 341
Fragments arranged by size:
3108 1024 341
Bme1580I G_kGCm'C
Cuts at: 0 729 2422 2952 4473
Size: 729 1693 530 1521
Fragments arranged by size:
1693 1521 729 530
BmrI ACTGGGnnnn_n'
Cuts at: 0 2298 2593 3013 3256 3632 3947 4473
Size: 2298 295 420 243 376 315 526
Fragments arranged by size:
2298 526 420 376 315 295 243
BmtI G_CTAG'C
Cuts at: 0 1436 3719 4473
Size: 1436 2283 754
Fragments arranged by size:
2283 1436 754
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2237 2367 4473
Size: 2237 130 2106
Fragments arranged by size:
2237 2106 130
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 699 797 1607 2448 2590 2775 4435 4473
Size: 699 98 810 841 142 185 1660 38
Fragments arranged by size:
1660 841 810 699 185 142 98 38
Bpu10I CC'TnA_GC
Cuts at: 0 1541 4473
Size: 1541 2932
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2207 3235 3919 4441 4473
Size: 2207 1028 684 522 32
Fragments arranged by size:
2207 1028 684 522 32
BsaI GGTCTCn'nnnn_
Cuts at: 0 1302 2208 2384 2764 4473
Size: 1302 906 176 380 1709
Fragments arranged by size:
1709 1302 906 380 176
BsaHI Gr'CG_yC
Cuts at: 0 1237 2310 4473
Size: 1237 1073 2163
Fragments arranged by size:
2163 1237 1073
BsaWI w'CCGG_w
Cuts at: 0 1148 2393 2822 4196 4400 4473
Size: 1148 1245 429 1374 204 73
Fragments arranged by size:
1374 1245 1148 429 204 73
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 230 260 4408 4438 4473
Size: 230 30 4148 30 35
Fragments arranged by size:
4148 230 35 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1241 1691 2338 2990 3244 3374 4473
Size: 1241 450 647 652 254 130 1099
Fragments arranged by size:
1241 1099 652 647 450 254 130
BseYI C'CCAG_C
Cuts at: 0 690 894 2254 3188 4473
Size: 690 204 1360 934 1285
Fragments arranged by size:
1360 1285 934 690 204
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 2458 4473
Size: 49 2409 2015
Fragments arranged by size:
2409 2015 49
BsiHKAI G_wGCw'C
Cuts at: 0 729 1080 2348 2422 2528 4278 4473
Size: 729 351 1268 74 106 1750 195
Fragments arranged by size:
1750 1268 729 351 195 106 74
BsmI GAATG_Cn'
Cuts at: 0 956 1042 2273 3116 4473
Size: 956 86 1231 843 1357
Fragments arranged by size:
1357 1231 956 843 86
Bsp1286I G_dGCh'C
Cuts at: 0 729 1080 2195 2261 2348 2422 2528 2952
Size: 729 351 1115 66 87 74 106 424
Cuts at: 2952 4278 4473
Size: 1326 195
Fragments arranged by size:
1326 1115 729 424 351 195 106 87
74 66
BspEI T'CCGG_A
Cuts at: 0 4196 4473
Size: 4196 277
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 117 2226 3372 4473
Size: 117 2109 1146 1101
Fragments arranged by size:
2109 1146 1101 117
BsrBI CCG'CTC
Cuts at: 0 947 4473
Size: 947 3526
BsrDI GCAATG_nn'
Cuts at: 0 313 557 2060 2864 4473
Size: 313 244 1503 804 1609
Fragments arranged by size:
1609 1503 804 313 244
BstXI CCAn_nnnn'nTGG
Cuts at: 0 666 2487 4450 4473
Size: 666 1821 1963 23
Fragments arranged by size:
1963 1821 666 23
BstYI r'GATC_y
Cuts at: 0 1834 2469 3108 3444 3673 3858 4132 4473
Size: 1834 635 639 336 229 185 274 341
Fragments arranged by size:
1834 639 635 341 336 274 229 185
Bsu36I CC'TnA_GG
Cuts at: 0 3977 4473
Size: 3977 496
BtgI C'CryG_G
Cuts at: 0 197 1478 4473
Size: 197 1281 2995
Fragments arranged by size:
2995 1281 197
BtsI GCAGTG_nn'
Cuts at: 0 19 701 712 1291 4473
Size: 19 682 11 579 3182
Fragments arranged by size:
3182 682 579 19 11
DraI TTT'AAA
Cuts at: 0 780 3045 3524 4181 4473
Size: 780 2265 479 657 292
Fragments arranged by size:
2265 780 657 479 292
DraIII CAC_nnn'GTG
Cuts at: 0 2737 4473
Size: 2737 1736
EaeI y'GGCC_r
Cuts at: 0 1288 1679 2446 3064 4473
Size: 1288 391 767 618 1409
Fragments arranged by size:
1409 1288 767 618 391
EarI CTCTTCn'nnn_
Cuts at: 0 1530 1586 1944 2131 3071 3149 3238 4473
Size: 1530 56 358 187 940 78 89 1235
Fragments arranged by size:
1530 1235 940 358 187 89 78 56
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 4351 4473
Size: 4351 122
EcoICRI GAG'CTC
Cuts at: 0 2346 4276 4473
Size: 2346 1930 197
Fragments arranged by size:
2346 1930 197
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 128 699 797 1607 1993 2155 2448 2590
Size: 128 571 98 810 386 162 293 142
Cuts at: 2590 2705 2775 3095 3164 3376 3722 4435 4473
Size: 115 70 320 69 212 346 713 38
Fragments arranged by size:
810 713 571 386 346 320 293 212
162 142 128 115 98 70 69 38
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2362 3939 4473
Size: 2362 1577 534
Fragments arranged by size:
2362 1577 534
EcoO109I rG'GnC_Cy
Cuts at: 0 429 4473
Size: 429 4044
EcoRI G'AATT_C
Cuts at: 0 3298 3872 4473
Size: 3298 574 601
Fragments arranged by size:
3298 601 574
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2960 4473
Size: 2960 1513
HaeII r_GCGC'y
Cuts at: 0 2313 4473
Size: 2313 2160
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1 33 1921 1953 2130 2162 2461 2493
Size: 1 32 1888 32 177 32 299 32
Cuts at: 2493 3933 3965 4473
Size: 1440 32 508
Fragments arranged by size:
1888 1440 508 299 177 32 32 32
32 32 1
HincII GTy'rAC
Cuts at: 0 1320 2382 4392 4473
Size: 1320 1062 2010 81
Fragments arranged by size:
2010 1320 1062 81
HindIII A'AGCT_T
Cuts at: 0 2995 4473
Size: 2995 1478
HpaI GTT'AAC
Cuts at: 0 1320 4473
Size: 1320 3153
KasI G'GCGC_C
Cuts at: 0 2309 4473
Size: 2309 2164
KpnI G_GTAC'C
Cuts at: 0 140 4473
Size: 140 4333
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 66 1843 3018 3598 4431 4473
Size: 66 1777 1175 580 833 42
Fragments arranged by size:
1777 1175 833 580 66 42
MscI TGG'CCA
Cuts at: 0 1681 4473
Size: 1681 2792
MslI CAynn'nnrTG
Cuts at: 0 56 843 2038 2485 4473
Size: 56 787 1195 447 1988
Fragments arranged by size:
1988 1195 787 447 56
MspA1I CmG'CkG
Cuts at: 0 1028 2251 4413 4473
Size: 1028 1223 2162 60
Fragments arranged by size:
2162 1223 1028 60
NarI GG'CG_CC
Cuts at: 0 2310 4473
Size: 2310 2163
NcoI C'CATG_G
Cuts at: 0 197 1478 4473
Size: 197 1281 2995
Fragments arranged by size:
2995 1281 197
NdeI CA'TA_TG
Cuts at: 0 756 4473
Size: 756 3717
NheI G'CTAG_C
Cuts at: 0 1432 3715 4473
Size: 1432 2283 758
Fragments arranged by size:
2283 1432 758
NsiI A_TGCA'T
Cuts at: 0 1044 2704 4473
Size: 1044 1660 1769
Fragments arranged by size:
1769 1660 1044
NspI r_CATG'y
Cuts at: 0 867 1629 2702 3631 4473
Size: 867 762 1073 929 842
Fragments arranged by size:
1073 929 867 842 762
PciI A'CATG_T
Cuts at: 0 863 4473
Size: 863 3610
PflMI CCAn_nnn'nTGG
Cuts at: 0 235 4473
Size: 235 4238
PfoI T'CCnGG_A
Cuts at: 0 1907 4442 4473
Size: 1907 2535 31
Fragments arranged by size:
2535 1907 31
PpuMI rG'GwC_Cy
Cuts at: 0 429 4473
Size: 429 4044
PsiI TTA'TAA
Cuts at: 0 904 4473
Size: 904 3569
PstI C_TGCA'G
Cuts at: 0 515 4468 4473
Size: 515 3953 5
Fragments arranged by size:
3953 515 5
PvuII CAG'CTG
Cuts at: 0 1028 2251 4413 4473
Size: 1028 1223 2162 60
Fragments arranged by size:
2162 1223 1028 60
SacI G_AGCT'C
Cuts at: 0 2348 4278 4473
Size: 2348 1930 195
Fragments arranged by size:
2348 1930 195
SapI GCTCTTCn'nnn_
Cuts at: 0 1530 1944 4473
Size: 1530 414 2529
Fragments arranged by size:
2529 1530 414
ScaI AGT'ACT
Cuts at: 0 1206 2567 4092 4473
Size: 1206 1361 1525 381
Fragments arranged by size:
1525 1361 1206 381
SfcI C'TryA_G
Cuts at: 0 107 367 511 4022 4344 4464 4473
Size: 107 260 144 3511 322 120 9
Fragments arranged by size:
3511 322 260 144 120 107 9
SfoI GGC'GCC
Cuts at: 0 2311 4473
Size: 2311 2162
SmlI C'TyrA_G
Cuts at: 0 2222 2232 2557 3214 3898 4456 4473
Size: 2222 10 325 657 684 558 17
Fragments arranged by size:
2222 684 657 558 325 17 10
SpeI A'CTAG_T
Cuts at: 0 1273 4473
Size: 1273 3200
SspI AAT'ATT
Cuts at: 0 253 1001 2064 3049 4473
Size: 253 748 1063 985 1424
Fragments arranged by size:
1424 1063 985 748 253
StyI C'CwwG_G
Cuts at: 0 197 659 1478 3582 3999 4473
Size: 197 462 819 2104 417 474
Fragments arranged by size:
2104 819 474 462 417 197
SwaI ATTT'AAAT
Cuts at: 0 3045 4473
Size: 3045 1428
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 704 2050 2104 2302 4020 4473
Size: 704 1346 54 198 1718 453
Fragments arranged by size:
1718 1346 704 453 198 54
TatI w'GTAC_w
Cuts at: 0 2 619 1204 2565 2630 4090 4473
Size: 2 617 585 1361 65 1460 383
Fragments arranged by size:
1460 1361 617 585 383 65 2
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 80 112 814 846 1222 1254 4473
Size: 80 32 702 32 376 32 3219
Fragments arranged by size:
3219 702 376 80 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 4387 4473
Size: 4387 86
XbaI T'CTAG_A
Cuts at: 0 1057 2340 4473
Size: 1057 1283 2133
Fragments arranged by size:
2133 1283 1057
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 447 666 1476 4473
Size: 447 219 810 2997
Fragments arranged by size:
2997 810 447 219
XmnI GAAnn'nnTTC
Cuts at: 0 1595 4058 4473
Size: 1595 2463 415
Fragments arranged by size:
2463 1595 415
ZraI GAC'GTC
Cuts at: 0 1238 4473
Size: 1238 3235
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflII AflIII AlwNI
ApaLI ApoI AseI AvaI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglII Bme1580I BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI
BspMI BsrBI BsrDI BstXI BstYI Bsu36I BtgI BtsI
DraI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NsiI NspI PciI PflMI PfoI PpuMI
PsiI PstI PvuII SacI SapI ScaI SfcI SfoI
SmlI SpeI SspI StyI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XmnI ZraI
Enzymes that do not cut:
AclI AfeI AgeI AhdI AleI AlfI AloI ApaI
AscI AsiSI AvrII BaeI BamHI BglI BlpI BmgBI
BsaAI BsaBI BsiEI BsiWI BsmBI BspHI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgZI ClaI
CspCI DrdI EagI EcoRV FseI FspI FspAI MfeI
MluI NaeI NgoMIV NotI NruI PacI PasI PmeI
PmlI PpiI PshAI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SanDI SbfI SexAI SfiI SgfI SgrAI
SmaI SnaBI SphI SrfI StuI XhoI XmaI